LOCUS       RC221887                7953 bp    DNA     circular  SYN 
DEFINITION  PSD (Myc-DDK-tagged)-Human pleckstrin and Sec7 domain containing (PSD)
ACCESSION   NM_002779
VERSION     NM_002779.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4110..4201 
                     /label="Myc-DDK"  
     misc_feature    4231..4816 
                     /label="PolyA signal"  
     misc_feature    4288..4307 
                     /label="Restriction Enzyme"  
     misc_feature    5092..5764 
                     /label="ColE1"  
     misc_feature    6194..6988 
                     /label="Kan/Neo"  
     misc_feature    7023..7325 
                     /label="SV40 ori"  
     misc_feature    7411..7888 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4092..4098 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4092 
                     /label="PSD"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCAGGGT GCCATGCGCT TCTGCTCGGA AGGCGACTGT GCCATCTCCC 
     1081 CACCACGATG CCCACGCCGC TGGCTCCCCG AAGGCCCGGT GCCCCAGAGC CCCCCAGCCA 
     1141 GCATGTATGG CAGCACAGGC TCCTTGCTAC GGCGAGTGGC AGGTCCAGGT CCTCGAGGCC 
     1201 GGGAACTGGG ACGTGTGACA GCACCCTGTA CACCTCTGCG TGGCCCCCCC TCACCCCGTG 
     1261 TTGCTCCCTC ACCCTGGGCA CCCTCTTCAC CCACTGGGCA GCCCCCACCC GGGGCCCAGA 
     1321 GCTCTGTGGT CATCTTCCGC TTTGTGGAGA AGGCCAGTGT GAGGCCACTG AATGGGCTAC 
     1381 CTGCTCCAGG GGGCTTGAGT CGGAGCTGGG ATCTGGGTGG GGTTTCTCCT CCCAGGCCCA 
     1441 CCCCAGCCCT TGGGCCTGGC TCCAACCGGA AGTTACGGCT GGAAGCATCC ACATCAGACC 
     1501 CACTCCCCGC CAGAGGAGGC TCGGCCCTAC CTGGCAGCCG GAACCTTGTA CATGGGCCGC 
     1561 CAGCCCCACC CCAGGTTGGA GCAGATGGCC TTTACTCCTC TCTCCCCAAT GGGCTGGGGG 
     1621 GCCCCCCTGA GCGCCTGGCC ACACTCTTCG GAGGACCTGC TGACACTGGA TTCCTGAACC 
     1681 AGGGGGATAC CTGGTCCTCC CCCCGGGAAG TCTCCTCTCA TGCCCAGAGA ATCGCTAGAG 
     1741 CCAAATGGGA ATTCTTCTAT GGCTCCTTGG ACCCCCCCAG CTCAGGTGCT AAGCCCCCAG 
     1801 AGCAGGCCCC CCCATCTCCA CCTGGGGTGG GCTCAAGGCA GGGCTCTGGG GTGGCTGTGG 
     1861 GGCGAGCAGC CAAGTACTCC GAGACAGACC TGGACACGGT GCCCCTGAGG TGCTACCGCG 
     1921 AGACTGACAT CGATGAGGTG CTGGCTGAGC GGGAGGAGGC CGACTCGGCC ATCGAAAGTC 
     1981 AGCCCAGCTC TGAGGGCCCA CCAGGCACTG CCTACCCACC TGCCCCACGG CCCGGCCCAC 
     2041 TCCCTGGCCC TCATCCCAGC CTCGGCAGTG GCAATGAGGA TGAGGACGAC GATGAGGCAG 
     2101 GTGGGGAAGA AGATGTGGAC GACGAGGTGT TTGAGGCCTC TGAAGGGGCC CGGCCAGGGA 
     2161 GCCGGATGCC TCTCAAGTCA CCTGTGCCCT TTCTACCTGG GACGAGCCCC TCGGCTGATG 
     2221 GGCCTGACTC TTTCAGTTGT GTGTTCGAAG CCATCCTGGA GTCACACCGG GCCAAAGGCA 
     2281 CCTCCTATAC CAGCCTCGCC TCGCTGGAGG CCTTGGCCTC ACCTGGCCCA ACCCAGAGCC 
     2341 CCTTCTTCAC CTTTGAGCTG CCTCCCCAAC CCCCTGCACC CCGGCCCGAC CCACCAGCTC 
     2401 CCGCCCCACT TGCCCCTCTT GAACCGGATT CTGGTACCAG CTCTGCTGCT GATGGTCCTT 
     2461 GGACACAGAG AGGGGAGGAG GAGGAGGCAG AGGCCAGAGC CAAGCTGGCC CCAGGGAGGG 
     2521 AGCCCCCTAG TCCCTGCCAC TCAGAGGACA GCCTTGGGCT GGGGGCAGCA CCCCTTGGCA 
     2581 GCGAACCACC CCTGAGCCAG CTGGTGTCCG ACTCAGACTC AGAGCTGGAC AGCACAGAGC 
     2641 GGCTGGCCCT GGGAAGCACA GACACCTTGT CCAATGGGCA GAAAGCGGAC CTGGAGGCTG 
     2701 CGCAGCGCCT GGCCAAGAGG CTGTACCGAC TAGATGGCTT CAGGAAGGCC GATGTGGCCC 
     2761 GGCACCTGGG CAAGAACAAT GACTTCAGCA AACTGGTGGC TGGGGAGTAC CTCAAGTTCT 
     2821 TTGTCTTCAC GGGCATGACT CTGGACCAAG CTCTCAGGGT GTTTCTGAAG GAGCTGGCCT 
     2881 TAATGGGTGA GACCCAGGAA CGAGAGCGCG TGCTGGCCCA CTTCTCCCAG CGATACTTCC 
     2941 AGTGCAATCC TGAAGCCCTG TCCTCAGAGG ACGGCGCCCA CACGCTGACC TGTGCGCTCA 
     3001 TGCTGCTCAA CACGGATCTC CACGGCCATA ACATCGGGAA GCGCATGACC TGCGGGGACT 
     3061 TCATCGGGAA CCTGGAGGGC CTCAATGATG GCGGCGACTT CCCTAGGGAG CTGCTCAAGG 
     3121 CCTTGTACAG CTCCATCAAG AATGAGAAGC TGCAGTGGGC CATAGACGAG GAGGAGCTGA 
     3181 GACGCTCTCT GTCTGAGTTG GCCGACCCCA ACCCCAAGGT CATCAAGCGG ATCAGCGGGG 
     3241 GCAGTGGCAG TGGCTCCAGC CCTTTCCTGG ACCTGACTCC CGAGCCTGGG GCTGCCGTCT 
     3301 ACAAGCACGG GGCCCTGGTG CGAAAGGTGC ACGCAGACCC TGACTGCAGG AAGACACCTC 
     3361 GGGGCAAGCG GGGCTGGAAG AGCTTCCACG GGATCCTCAA GGGCATGATC CTCTACCTGC 
     3421 AGAAGGAGGA GTACAAGCCT GGGAAGGCCC TTTCAGAGAC GGAGCTCAAG AATGCCATCA 
     3481 GCATCCACCA TGCCCTGGCC ACTCGTGCCA GTGACTACAG CAAGAGGCCC CACGTCTTCT 
     3541 ACCTGCGCAC AGCTGACTGG CGGGTCTTCC TCTTCCAGGC CCCGAGCCTG GAGCAGATGC 
     3601 AGTCCTGGAT CACTCGCATC AATGTAGTAG CCGCTATGTT CTCTGCGCCC CCCTTCCCAG 
     3661 CTGCTGTTAG CTCCCAAAAG AAGTTCAGCC GCCCTCTCCT GCCCAGCGCT GCCACCCGCC 
     3721 TCTCCCAGGA GGAGCAGGTG CGGACCCACG AGGCCAAGCT GAAGGCCATG GCAAGTGAGC 
     3781 TGCGGGAGCA CCGGGCCGCC CAGCTGGGCA AGAAGGGCCG GGGCAAGGAG GCTGAAGAGC 
     3841 AGCGGCAGAA GGAGGCCTAC CTGGAGTTTG AGAAATCCCG CTACAGCACC TATGCAGCGC 
     3901 TGCTTCGGGT CAAGCTGAAG GCAGGCAGTG AGGAGCTGGA TGCAGTGGAG GCAGCACTGG 
     3961 CCCAGGCCGG GAGCACAGAG GATGGACTCC CTCCTTCTCA CTCCAGTCCC TCCCTGCAGC 
     4021 CCAAACCCTC CAGCCAGCCC CGGGCTCAGC GTCACAGCTC AGAGCCTCGG CCAGGGGCAG 
     4081 GCAGTGGGCG GCGGAAGCCC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4141 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4201 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4261 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4321 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4381 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4441 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4501 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4561 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4621 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4681 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4741 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4801 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4861 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4921 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4981 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5041 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5101 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5161 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5221 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5281 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5341 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5401 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5461 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5521 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5581 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5641 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5701 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5761 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5821 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5881 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5941 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6001 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6061 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6121 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6181 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6241 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6301 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6361 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6421 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6481 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6541 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6601 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6661 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6721 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6781 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6841 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6901 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6961 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7021 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7081 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7141 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7201 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7261 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7321 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7381 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7441 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7501 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7561 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7621 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7681 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7741 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7801 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7861 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7921 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//