LOCUS       RC221880                9060 bp    DNA     circular  SYN 
DEFINITION  SBNO1 (Myc-DDK-tagged)-Human strawberry notch homolog 1 (Drosophila) (SBNO1), transcript variant 2
ACCESSION   NM_018183
VERSION     NM_018183.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5217..5308 
                     /label="Myc-DDK"  
     misc_feature    5338..5923 
                     /label="PolyA signal"  
     misc_feature    5395..5414 
                     /label="Restriction Enzyme"  
     misc_feature    6199..6871 
                     /label="ColE1"  
     misc_feature    7301..8095 
                     /label="Kan/Neo"  
     misc_feature    8130..8432 
                     /label="SV40 ori"  
     misc_feature    8518..8995 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5199..5205 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5199 
                     /label="SBNO1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGAGCCA GGGCAAGATT TACTGCTTGC TGCTTTGAGT GAGAGTGGAA 
     1081 TTAGTCCGAA TGACCTCTTT GATATTGATG GTGGAGATGC AGGGCTTGCA ACTCCAATGC 
     1141 CTACCCCGTC AGTTCAGCAG TCAGTGCCAC TTAGTGCATT AGAACTAGGT TTGGAGACCG 
     1201 AAGCAGCAGT TCCTGTTAAA CAAGAACCAG AGACTGTACC TACTCCAGCA CTATTAAATG 
     1261 TGAGGCAGCA GCCTCCATCT ACTACAACAT TTGTGCTGAA TCAAATAAAT CATCTTCCAC 
     1321 CCTTGGGATC TACAATTGTA ATGACTAAAA CACCACCTGT AACAACCAAC AGGCAAACCA 
     1381 TCACTTTAAC TAAGTTTATC CAGACTACTG CAAGCACACG CCCGTCAGTC TCAGCACCAA 
     1441 CAGTACGAAA TGCCATGACC TCTGCACCTT CAAAAGACCA AGTTCAGCTT AAAGATCTAC 
     1501 TGAAAAATAA TAGTCTTAAT GAACTGATGA AACTAAAGCC ACCTGCTAAT ATTGCTCAGC 
     1561 CAGTAGCAAC AGCAGCTACT GATGTAAGCA ATGGTACAGT AAAGAAAGAG TCTTCTAATA 
     1621 AAGAAGGAGC TAGAATGTGG ATAAACGACA TGAAGATGAG GAGTTTTTCC CCAACCATGA 
     1681 AGGTTCCTGT TGTAAAAGAA GATGATGAAC CAGAGGAAGA AGATGAAGAA GAAATGGGTC 
     1741 ATGCAGAAAC CTATGCAGAA TACATGCCAA TAAAATTAAA AATTGGCCTA CGTCATCCAG 
     1801 ATGCTGTAGT GGAAACCAGC TCTTTATCCA GTGTTACTCC TCCTGATGTT TGGTACAAAA 
     1861 CATCCATTTC TGAGGAAACC ATTGATAATG GCTGGTTATC AGCATTGCAG CTTGAGGCAA 
     1921 TTACATATGC AGCCCAGCAA CATGAAACTT TCCTACCTAA TGGAGATCGT GCTGGCTTCT 
     1981 TAATAGGTGA TGGTGCCGGT GTAGGAAAAG GAAGGACGAT AGCAGGAATC ATCTATGAAA 
     2041 ATTATTTGTT GAGTAGAAAA CGAGCATTGT GGTTTAGTGT TTCAAATGAC TTAAAGTATG 
     2101 ATGCTGAAAG AGATTTAAGG GATATTGGAG CAAAAAACAT TTTGGTTCAT TCGTTAAATA 
     2161 AGTTTAAATA CGGAAAAATT TCTTCCAAAC ATAATGGGAG TGTGAAAAAG GGTGTTATTT 
     2221 TTGCTACTTA CTCTTCACTT ATTGGTGAAA GCCAGTCTGG CGGCAAGTAT AAAACTAGGT 
     2281 TAAAACAACT TCTGCATTGG TGCGGTGATG ACTTCGATGG AGTGATAGTG TTTGATGAGT 
     2341 GTCATAAAGC CAAAAACTTA TGTCCTGTTG GTTCTTCAAA GCCAACCAAG ACAGGCTTAG 
     2401 CAGTTTTAGA GCTTCAGAAC AAATTGCCAA AAGCCAGAGT TGTTTATGCT AGTGCAACTG 
     2461 GTGCTTCTGA ACCACGCAAC ATGGCCTATA TGAACCGTCT TGGCATATGG GGTGAGGGTA 
     2521 CTCCATTTAG AGAATTCAGT GATTTTATTC AAGCAGTAGA ACGGAGAGGA GTTGGTGCCA 
     2581 TGGAAATAGT TGCTATGGAT ATGAAGCTTA GAGGAATGTA CATTGCTCGA CAACTGAGCT 
     2641 TTACTGGAGT GACCTTCAAA ATTGAGGAAG TTCTTCTTTC TCAGAGCTAC GTTAAAATGT 
     2701 ATAACAAAGC TGTCAAGCTG TGGGTCATCG CCAGAGAGCG GTTTCAGCAA GCTGCAGATC 
     2761 TGATTGATGC TGAGCAACGA ATGAAGAAGT CCATGTGGGG TCAGTTCTGG TCTGCTCACC 
     2821 AGAGGTTCTT CAAATACTTA TGCATAGCAT CCAAAGTTAA AAGGGTTGTG CAACTAGCTC 
     2881 GAGAGGAAAT CAAGAATGGA AAATGTGTTG TAATTGGTCT GCAGTCTACA GGAGAAGCTA 
     2941 GAACATTAGA AGCTTTGGAA GAGGGCGGGG GAGAATTGAA TGATTTTGTT TCAACTGCCA 
     3001 AAGGTGTGTT GCAGTCACTC ATTGAAAAAC ATTTTCCTGC TCCAGACAGG AAAAAACTTT 
     3061 ATAGTTTACT AGGAATCGAT TTGACAGCTC CAAGTAACAA CAGTTCGCCA AGAGATAGTC 
     3121 CTTGTAAAGA AAATAAAATA AAGAAGCGGA AAGGTGAAGA AATAACTCGA GAAGCCAAAA 
     3181 AAGCACGAAA AGTAGGTGGC CTTACTGGTA GCAGTTCTGA CGACAGTGGA AGTGAATCTG 
     3241 ATGCCTCTGA TAATGAAGAA AGTGACTATG AGAGCTCTAA AAACATGAGT TCTGGAGATG 
     3301 ATGACGATTT CAACCCATTT TTAGATGAGT CTAATGAGGA TGATGAAAAT GATCCCTGGT 
     3361 TAATTAGAAA AGACCACAAG AAAAACAAAG AGAAAAAAAA GAAGAAAAGT ATAGATCCAG 
     3421 ATTCTATTCA AAGTGCCTTA TTAGCATCAG GTCTTGGATC AAAACGACCT AGTTTTTCAT 
     3481 CTACACCAGT TATCTCACCT GCTCCTAACA GTACACCAGC TAACAGTAAC ACCAACAGTA 
     3541 ACAGTAGCCT TATAACAAGT CAGGATGCTG TGGAAAGGGC TCAGCAGATG AAGAAAGACC 
     3601 TGCTTGATAA GCTAGAAAAA TTAGCTGAAG ACCTCCCCCC TAATACCCTG GATGAACTTA 
     3661 TCGATGAACT TGGTGGCCCT GAGAACGTTG CTGAGATGAC TGGCCGCAAG GGACGGGTTG 
     3721 TGAGCAATGA TGATGGAAGC ATATCTTATG AGTCAAGATC TGAACTTGAT GTGCCTGTGG 
     3781 AAATACTAAA CATCACAGAA AAACAACGAT TTATGGATGG AGATAAGAAT ATTGCTATCA 
     3841 TCTCAGAAGC TGCCAGCTCG GGTATTTCAT TACAAGCAGA TAGGAGAGCT AAAAATCAAA 
     3901 GGCGAAGAGT TCATATGACT TTAGAATTAC CTTGGAGCGC TGATAGAGCA ATTCAACAGT 
     3961 TCGGACGTAC TCATAGATCA AACCAAGTTA CTGCTCCTGA GTATGTCTTT CTGATATCTG 
     4021 AACTGGCAGG AGAACAAAGA TTTGCATCTA TTGTTGCTAA AAGACTTGAG AGTTTGGGGG 
     4081 CACTTACACA TGGCGATAGA AGGGCAACAG AATCTAGAGA TCTGAGCAGG TTCAACTTTG 
     4141 ATAATAAGTA TGGAAGAAAT GCTTTAGAAA TTGTCATGAA ATCCATTGTA AACTTGGATT 
     4201 CTCCTATGGT ATCACCACCT CCAGACTATC CTGGAGAATT TTTTAAAGAT GTTCGACAAG 
     4261 GACTGATAGG CGTTGGCCTG ATAAATGTAG AAGATCGCTC GGGAATTCTT ACTCTCGATA 
     4321 AAGATTATAA CAACATAGGA AAATTCTTAA ATAGAATTTT AGGCATGGAG GTGCATCAGC 
     4381 AGAATGCGTT ATTTCAGTAT TTTGCGGACA CACTTACTGC AGTTGTTCAA AATGCCAAAA 
     4441 AAAATGGAAG ATATGATATG GGAATCTTAG ATCTTGGTTC TGGAGATGAA AAAGTGCGGA 
     4501 AAAGTGATGT TAAAAAGTTT CTGACTCCAG GATATTCAAC CTCTGGCCAC GTAGAATTAT 
     4561 ACACAATTAG TGTAGAGAGG GGAATGTCAT GGGAGGAAGC TACCAAGATT TGGGCTGAGC 
     4621 TGACAGGACC AGACGATGGC TTTTACTTGT CATTGCAAAT AAGGAACAAC AAGAAAACTG 
     4681 CCATCTTAGT TAAAGAAGTG AATCCTAAAA AGAAACTTTT CTTAGTTTAT CGACCAAATA 
     4741 CTGGGAAGCA GCTCAAATTA GAAATTTATG CTGATCTAAA AAAGAAATAT AAGAAGGTCG 
     4801 TCTCAGATGA TGCCCTGATG CACTGGTTAG ATCAGTATAA TTCATCTGCA GATACTTGTA 
     4861 CTCATGCTTA TTGGCGCGGC AATTGCAAAA AAGCAAGCTT GGGGCTAGTT TGTGAAATAG 
     4921 GTCTTCGTTG CCGTACATAT TATGTATTAT GTGGTTCAGT GCTGAGTGTC TGGACAAAAG 
     4981 TTGAGGGTGT TCTAGCATCT GTCAGTGGCA CAAACGTGAA GATGCAGATC GTGCGGCTAA 
     5041 GAACGGAAGA TGGGCAACGG ATTGTAGGTT TGATCATTCC GGCAAATTGT GTGTCTCCTC 
     5101 TTGTAAATCT CCTATCAACT TCAGACCAGT CTCAACAGCT TGCGGTCCAA CAGAAACAGC 
     5161 TATGGCAACA GCATCACCCT CAGAGCATCA CCAACTTGAG CAACGCAACG CGTACGCGGC 
     5221 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5281 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5341 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5401 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5461 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5521 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5581 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5641 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5701 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5761 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5821 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5881 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5941 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     6001 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     6061 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     6121 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     6181 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6241 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6301 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6361 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6421 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6481 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6541 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6601 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6661 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6721 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6781 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6841 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6901 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6961 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     7021 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     7081 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     7141 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     7201 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7261 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7321 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7381 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7441 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7501 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7561 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7621 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7681 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7741 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7801 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7861 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7921 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7981 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     8041 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     8101 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     8161 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     8221 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8281 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8341 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8401 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8461 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8521 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8581 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8641 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8701 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8761 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8821 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8881 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8941 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     9001 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//