LOCUS       RC221824                6633 bp    DNA     circular  SYN 
DEFINITION  C8A (Myc-DDK-tagged)-Human complement component 8, alpha polypeptide (C8A)
ACCESSION   NM_000562
VERSION     NM_000562.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2790..2881 
                     /label="Myc-DDK"  
     misc_feature    2911..3496 
                     /label="PolyA signal"  
     misc_feature    2968..2987 
                     /label="Restriction Enzyme"  
     misc_feature    3772..4444 
                     /label="ColE1"  
     misc_feature    4874..5668 
                     /label="Kan/Neo"  
     misc_feature    5703..6005 
                     /label="SV40 ori"  
     misc_feature    6091..6568 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2772..2778 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2772 
                     /label="C8A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTGCTGTT GTTTTCTTCA TCTTGTCTTT GATGACTTGT CAGCCTGGGG 
     1081 TAACTGCACA GGAGAAGGTG AACCAGAGAG TAAGACGGGC AGCTACACCC GCAGCAGTTA 
     1141 CCTGCCAGCT GAGCAACTGG TCAGAGTGGA CAGATTGCTT TCCGTGCCAG GACAAAAAGT 
     1201 ACCGACACCG GAGCCTCTTG CAGCCAAACA AGTTTGGGGG AACCATCTGC AGTGGTGACA 
     1261 TCTGGGATCA AGCCAGCTGC TCCAGTTCTA CAACTTGTGT AAGGAAAGCA CAGTGTGGAC 
     1321 AGGATTTCCA GTGTAAGGAG ACAGGTCGCT GCCTGAAACG CCACCTTGTG TGTAATGGAG 
     1381 ACCAGGACTG CCTTGATGGC TCTGATGAGG ACGACTGTGA AGATGTCAGG GCCATTGACG 
     1441 AAGACTGCAG CCAGTATGAA CCAATTCCAG GATCACAGAA GGCAGCCTTG GGGTACAATA 
     1501 TCCTGACCCA GGAAGATGCT CAGAGTGTGT ACGATGCCAG TTATTATGGG GGCCAGTGTG 
     1561 AGACGGTATA CAATGGGGAA TGGAGGGAGC TTCGATATGA CTCCACCTGT GAACGTCTCT 
     1621 ACTATGGAGA TGATGAGAAA TACTTTCGGA AACCCTACAA CTTTCTGAAG TACCACTTTG 
     1681 AAGCCCTGGC AGATACTGGA ATCTCCTCAG AGTTTTATGA TAATGCAAAT GACCTTCTTT 
     1741 CCAAAGTTAA AAAAGACAAG TCTGACTCAT TTGGAGTGAC CATCGGCATA GGCCCAGCCG 
     1801 GCAGCCCTTT ATTGGTGGGT GTAGGTGTAT CCCACTCACA AGACACTTCA TTCTTGAACG 
     1861 AATTAAACAA GTATAATGAG AAGAAATTCA TTTTCACAAG AATCTTCACA AAGGTGCAGA 
     1921 CTGCACATTT TAAGATGAGG AAGGATGACA TTATGCTGGA TGAAGGAATG CTGCAGTCAT 
     1981 TAATGGAGCT TCCAGATCAG TACAATTATG GCATGTATGC CAAGTTCATC AATGACTATG 
     2041 GCACCCATTA CATCACATCT GGATCCATGG GTGGCATTTA TGAATATATC CTGGTGATTG 
     2101 ACAAAGCAAA AATGGAATCC CTTGGTATTA CCAGCAGAGA TATCACGACA TGTTTTGGAG 
     2161 GCTCCTTGGG CATTCAATAT GAAGACAAAA TAAATGTTGG TGGAGGTTTA TCAGGAGACC 
     2221 ATTGTAAAAA ATTTGGAGGT GGCAAAACTG AAAGGGCCAG GAAGGCCATG GCTGTGGAAG 
     2281 ACATTATTTC TCGGGTGCGA GGTGGCAGTT CTGGCTGGAG CGGTGGCTTG GCACAGAACA 
     2341 GGAGCACCAT TACATACCGT TCCTGGGGGA GGTCATTAAA GTATAATCCT GTTGTTATCG 
     2401 ATTTTGAGAT GCAGCCTATC CACGAGGTGC TGCGGCACAC AAGCCTGGGG CCTCTGGAGG 
     2461 CCAAGCGCCA GAACCTGCGC CGCGCCTTGG ACCAGTATCT GATGGAATTC AATGCCTGCC 
     2521 GATGTGGGCC TTGCTTCAAC AATGGGGTGC CCATCCTCGA GGGCACCAGC TGCAGGTGCC 
     2581 AGTGCCGCCT GGGTAGCTTG GGTGCTGCCT GTGAGCAAAC ACAGACAGAA GGAGCCAAAG 
     2641 CAGATGGGAG CTGGAGTTGC TGGAGCTCCT GGTCTGTATG CAGAGCAGGC ATCCAGGAAA 
     2701 GGAGAAGAGA GTGTGACAAT CCAGCACCTC AGAATGGAGG GGCCTCGTGT CCAGGGCGGA 
     2761 AAGTACAGAC GCAGGCTTGC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     2821 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     2881 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     2941 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3001 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3061 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3121 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3181 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3241 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3301 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     3361 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     3421 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     3481 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     3541 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     3601 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     3661 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     3721 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     3781 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     3841 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     3901 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     3961 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4021 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4081 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4141 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4201 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4261 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4321 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     4381 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     4441 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     4501 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     4561 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     4621 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     4681 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     4741 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     4801 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     4861 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     4921 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     4981 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5041 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5101 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5161 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5221 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5281 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     5341 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     5401 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     5461 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     5521 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     5581 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     5641 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     5701 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     5761 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     5821 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     5881 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     5941 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6001 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6061 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6121 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6181 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6241 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6301 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     6361 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     6421 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     6481 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     6541 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     6601 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//