LOCUS       RC221770L1                9528 bp    DNA     circular  SYN 
DEFINITION  MECOM (Myc-DDK-tagged)-Human MDS1 and EVI1 complex locus (MECOM), transcript variant 2
ACCESSION   NM_005241
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7819..7999 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8727..9386 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    8158..8726 
                     /label="ColE1"  
     misc_feature    6694..6714 
                     /label="LR50 primer"  
     misc_feature    6491..6562 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6703..7294 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6473..6479 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6473 
                     /label="MECOM"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAAGAGCGAA GACTATCCCC ATGAAACTAT 
     3361 GGCGCCGGAT ATCCACGAAG AACGGCAATA TCGCTGCGAA GACTGTGACC AGCTCTTTGA 
     3421 ATCTAAGGCT GAACTAGCAG ATCACCAAAA GTTTCCATGC AGTACTCCTC ACTCAGCATT 
     3481 TTCAATGGTT GAAGAGGACT TTCAGCAAAA ACTCGAAAGC GAGAATGATC TCCAAGAGAT 
     3541 ACACACGATC CAGGAGTGTA AGGAATGTGA CCAAGTTTTT CCTGATTTGC AAAGCCTGGA 
     3601 GAAACACATG CTGTCACATA CTGAAGAGAG GGAATACAAG TGTGATCAGT GTCCCAAGGC 
     3661 ATTTAACTGG AAGTCCAATT TAATTCGCCA CCAGATGTCA CATGACAGTG GAAAGCACTA 
     3721 TGAATGTGAA AACTGTGCCA AGGTTTTCAC GGACCCTAGC AACCTTCAGC GGCACATTCG 
     3781 CTCTCAGCAT GTCGGTGCCC GGGCCCATGC ATGCCCGGAG TGTGGCAAAA CGTTTGCCAC 
     3841 TTCGTCGGGC CTCAAACAAC ACAAGCACAT CCACAGCAGT GTGAAGCCCT TTATCTGTGA 
     3901 GGTCTGCCAT AAATCCTATA CTCAGTTTTC AAACCTTTGC CGTCATAAGC GCATGCATGC 
     3961 TGATTGCAGA ACCCAAATCA AGTGCAAAGA CTGTGGACAA ATGTTCAGCA CTACGTCTTC 
     4021 CTTAAATAAA CACAGGAGGT TTTGTGAGGG CAAGAACCAT TTTGCGGCAG GTGGATTTTT 
     4081 TGGCCAAGGC ATTTCACTTC CTGGAACCCC AGCTATGGAT AAAACGTCCA TGGTTAATAT 
     4141 GAGTCATGCC AACCCGGGCC TTGCTGACTA TTTTGGCGCC AATAGGCATC CTGCTGGTCT 
     4201 TACCTTTCCA ACAGCTCCTG GATTTTCTTT TAGCTTCCCT GGTCTGTTTC CTTCCGGCTT 
     4261 GTACCACAGG CCTCCTTTGA TACCTGCTAG TTCTCCTGTT AAAGGACTAT CAAGTACTGA 
     4321 ACAGACAAAC AAAAGTCAAA GTCCCCTCAT GACACATCCT CAGATACTGC CAGCTACACA 
     4381 GGATATTTTG AAGGCACTAT CTAAACACCC ATCTGTAGGG GACAATAAGC CAGTGGAGCT 
     4441 CCAGCCCGAG AGGTCCTCTG AAGAGAGGCC CTTTGAGAAA ATCAGTGACC AGTCAGAGAG 
     4501 TAGTGACCTT GATGATGTCA GTACACCAAG TGGCAGTGAC CTGGAAACAA CCTCGGGCTC 
     4561 TGATCTGGAA AGTGACATTG AAAGTGATAA AGAGAAATTT AAAGAAAATG GTAAAATGTT 
     4621 CAAAGACAAA GTAAGCCCTC TTCAGAATCT GGCTTCAATA AATAATAAGA AAGAATACAG 
     4681 CAATCATTCC ATTTTCTCAC CATCTTTAGA GGAGCAGACT GCGGTGTCAG GAGCTGTGAA 
     4741 TGATTCTATA AAGGCTATTG CTTCTATTGC TGAAAAATAC TTTGGTTCAA CAGGACTGGT 
     4801 GGGGCTGCAA GACAAAAAAG TTGGAGCTTT ACCTTACCCT TCCATGTTTC CCCTCCCATT 
     4861 TTTTCCAGCA TTCTCTCAAT CAATGTACCC ATTTCCTGAT AGAGACTTGA GATCGTTACC 
     4921 TTTGAAAATG GAACCCCAAT CACCAGGTGA AGTAAAGAAA CTGCAGAAGG GCAGCTCTGA 
     4981 GTCCCCCTTT GATCTCACCA CTAAGCGAAA GGATGAGAAG CCCTTGACTC CAGTCCCCTC 
     5041 CAAGCCTCCA GTGACACCTG CCACAAGCCA AGACCAGCCC CTGGATCTAA GTATGGGCAG 
     5101 TAGGAGTAGA GCCAGTGGGA CAAAGCTGAC TGAGCCTCGA AAAAACCACG TGTTTGGGGG 
     5161 AAAAAAAGGA AGCAACGTCG AATCAAGACC TGCTTCAGAT GGTTCCTTGC AGCATGCAAG 
     5221 ACCCACTCCT TTCTTTATGG ACCCTATTTA CAGAGTAGAG AAAAGAAAAC TAACTGACCC 
     5281 ACTTGAAGCT TTAAAAGAGA AATACTTGAG GCCTTCTCCA GGATTCTTGT TTCACCCACA 
     5341 ATTCCAACTG CCTGATCAGA GAACTTGGAT GTCAGCTATT GAAAACATGG CAGAAAAGCT 
     5401 AGAGAGCTTC AGTGCCCTGA AACCTGAGGC CAGTGAGCTC TTACAGTCAG TGCCCTCTAT 
     5461 GTTCAACTTC AGGGCGCCTC CCAATGCCCT GCCAGAGAAC CTTCTGCGGA AGGGAAAGGA 
     5521 GCGCTATACC TGCAGATACT GTGGCAAGAT TTTTCCAAGG TCTGCAAACC TAACACGGCA 
     5581 CTTGAGAACC CACACAGGAG AGCAGCCTTA CAGATGCAAA TACTGTGACA GATCATTTAG 
     5641 CATATCTTCT AACTTGCAAA GGCATGTTCG CAACATCCAC AATAAAGAGA AGCCATTTAA 
     5701 GTGTCACTTA TGTGATAGGT GTTTTGGTCA ACAAACCAAT TTAGACAGAC ACCTAAAGAA 
     5761 ACATGAGAAT GGGAACATGT CCGGTACAGC AACATCGTCG CCTCATTCTG AACTGGAAAG 
     5821 TACAGGTGCG ATTCTGGATG ACAAAGAAGA TGCTTACTTC ACAGAAATTC GAAATTTCAT 
     5881 TGGGAACAGC AACCATGGCA GCCAATCTCC CAGGAATGTG GAGGAGAGAA TGAATGGCAG 
     5941 TCATTTTAAA GATGAAAAGG CTTTGGTGAC CAGTCAAAAT TCAGACTTGC TGGATGATGA 
     6001 AGAAGTTGAA GATGAGGTGT TGTTAGATGA GGAGGATGAA GACAATGATA TTACTGGAAA 
     6061 AACAGGAAAG GAACCAGTGA CAAGTAATTT ACATGAAGGA AACCCTGAGG ATGACTATGA 
     6121 AGAAACCAGT GCCCTGGAGA TGAGTTGCAA GACATCCCCA GTGAGGTATA AAGAGGAAGA 
     6181 ATATAAAAGT GGACTTTCTG CTCTAGATCA TATAAGGCAC TTCACAGATA GCCTCAAAAT 
     6241 GAGGAAAATG GAAGATAATC AATATTCTGA AGCTGAGCTG TCTTCTTTTA GTACTTCCCA 
     6301 TGTGCCAGAG GAACTTAAGC AGCCGTTACA CAGAAAGTCC AAATCGCAGG CATATGCTAT 
     6361 GATGCTGTCA CTGTCTGACA AGGAGTCCCT CCATTCTACA TCCCACAGTT CTTCCAACGT 
     6421 GTGGCACAGT ATGGCCAGGG CTGCGGCGGA ATCCAGTGCT ATCCAGTCCA TAAGCCACGT 
     6481 AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA 
     6541 TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCTGTACAA 
     6601 GTAGGATTCG TCGAGGGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATACAT 
     6661 GTTTAAGGGT TCCGGTTCCA CTAGGTACAA TTCGATATCA AGCTTATCGA TAATCAACCT 
     6721 CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG 
     6781 CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC 
     6841 ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT 
     6901 GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC 
     6961 ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG 
     7021 GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT 
     7081 GACAATTCCG TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT 
     7141 GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG 
     7201 GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC 
     7261 CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC ATCGATACCG TCGACCTCGA 
     7321 TCGAGACCTA GAAAAACATG GAGCAATCAC AAGTAGCAAT ACAGCAGCTA CCAATGCTGA 
     7381 TTGTGCCTGG CTAGAAGCAC AAGAGGAGGA GGAGGTGGGT TTTCCAGTCA CACCTCAGGT 
     7441 ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA 
     7501 GGGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAGACAA GATATCCTTG ATCTGTGGAT 
     7561 CTACCACACA CAAGGCTACT TCCCTGATTG GCAGAACTAC ACACCAGGGC CAGGGATCAG 
     7621 ATATCCACTG ACCTTTGGAT GGTGCTACAA GCTAGTACCA GTTGAGCAAG AGAAGGTAGA 
     7681 AGAAGCCAAT GAAGGAGAGA ACACCCGCTT GTTACACCCT GTGAGCCTGC ATGGGATGGA 
     7741 TGACCCGGAG AGAGAAGTAT TAGAGTGGAG GTTTGACAGC CGCCTAGCAT TTCATCACAT 
     7801 GGCCCGAGAG CTGCATCCGG ACTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG 
     7861 GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT 
     7921 GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC 
     7981 CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     8041 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     8101 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     8161 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     8221 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     8281 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     8341 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     8401 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     8461 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     8521 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     8581 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     8641 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     8701 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGATTACG CCCCGCCCTG CCACTCATCG 
     8761 CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA AACGGCATGA 
     8821 TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG 
     8881 GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA 
     8941 CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT AGGGAAATAG 
     9001 GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA 
     9061 TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG 
     9121 TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC CATACGGAAC 
     9181 TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC 
     9241 TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT CTGGTTATAG 
     9301 GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA 
     9361 TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATACTCT TCCTTTTTCA ATATTATTGA 
     9421 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     9481 AAACAAATAG GGGTCCCGCG CACATTTCCC CGAAAAGTGC CACCTGAC
//