LOCUS       RC221769                8466 bp    DNA     circular  SYN 
DEFINITION  IGSF3 (Myc-DDK-tagged)-Human immunoglobulin superfamily, member 3 (IGSF3), transcript variant 2
ACCESSION   NM_001007237
VERSION     NM_001007237.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4623..4714 
                     /label="Myc-DDK"  
     misc_feature    4744..5329 
                     /label="PolyA signal"  
     misc_feature    4801..4820 
                     /label="Restriction Enzyme"  
     misc_feature    5605..6277 
                     /label="ColE1"  
     misc_feature    6707..7501 
                     /label="Kan/Neo"  
     misc_feature    7536..7838 
                     /label="SV40 ori"  
     misc_feature    7924..8401 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4605..4611 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4605 
                     /label="IGSF3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGTGCTTT TTCCCGGTGC TGAGCTGTCT GGCTGTGCTG GGTGTGGTGT 
     1081 CAGCACAGCG GCAGGTCACC GTTCAGGAAG GACCCTTGTA CCGCACGGAG GGCTCCCACA 
     1141 TCACTATCTG GTGCAATGTG AGTGGCTACC AGGGACCTTC TGAGCAGAAT TTCCAGTGGT 
     1201 CCATTTACCT GCCTTCGTCG CCAGAGCGAG AGGTGCAGAT CGTCAGCACC ATGGACTCTT 
     1261 CCTTCCCCTA TGCCATCTAC ACCCAGCGCG TCCGCGGAGG GAAGATCTTC ATAGAAAGAG 
     1321 TCCAGGGGAA CTCAACCCTA TTGCACATCA CAGATCTTCA GGCCCGGGAT GCCGGGGAGT 
     1381 ATGAATGCCA CACACCCAGC ACTGATAAGC AATACTTTGG GAGTTACAGT GCAAAGATGA 
     1441 ACCTAGTGGT GATCCCAGAC TCCCTGCAGA CCACTGCCAT GCCCCAGACT CTGCACAGAG 
     1501 TGGAGCAGGA CCCGCTGGAG CTCACTTGTG AGGTGGCCTC AGAGACCATT CAGCACAGCC 
     1561 ACCTGTCTGT GGCCTGGCTC CGGCAGAAAG TTGGCGAGAA GCCCGTGGAG GTCATCTCCC 
     1621 TGAGCCGAGA TTTCATGCTT CACTCCAGCA GCGAATATGC CCAGAGGCAG AGCCTGGGGG 
     1681 AGGTGCGGCT GGACAAGCTG GGGAGGACCA CCTTCCGCCT CACCATCTTC CACCTGCAGC 
     1741 CTTCTGACCA GGGCGAATTC TACTGCGAGG CCGCCGAGTG GATCCAGGAT CCGGATGGGT 
     1801 CGTGGTATGC TATGACCCGA AAGCGTTCCG AGGGAGCCGT GGTCAACGTC CAGCCAACTG 
     1861 ACAAAGAATT CACTGTTCGG CTGGAGACAG AGAAGCGGCT GCACACGGTG GGCGAGCCGG 
     1921 TGGAGTTCAG ATGCATCCTG GAGGCTCAGA ATGTTCCCGA CCGTTACTTT GCTGTCTCCT 
     1981 GGGCCTTCAA CAGCTCGCTC ATCGCCACCA TGGGTCCTAA CGCTGTGCCT GTCCTCAACA 
     2041 GCGAATTTGC TCACCGGGAA GCCAGGGGAC AGCTTAAGGT GGCCAAAGAG AGCGACAGTG 
     2101 TCTTTGTGCT GAAGATCTAC CACCTCCGCC AGGAAGATAG CGGGAAATAC AACTGCCGGG 
     2161 TGACTGAGCG AGAGAAAACC GTGACCGGGG AATTCATTGA TAAGGAGAGC AAGCGTCCCA 
     2221 AGAACATCCC CATCATAGTC CTCCCCCTCA AGAGCAGCAT CTCCGTGGAG GTGGCCAGCA 
     2281 ATGCCAGCGT CATCCTTGAG GGCGAGGACC TGCGCTTCTC CTGCAGTGTC CGCACGGCAG 
     2341 GCAGGCCGCA GGGTCGCTTC TCTGTCATCT GGCAGCTTGT GGACAGGCAG AACCGCCGCA 
     2401 GCAATATCAT GTGGCTAGAC CGGGATGGCA CCGTGCAGCC AGGCTCGTCC TACTGGGAGC 
     2461 GCAGCAGCTT TGGGGGCGTC CAGATGGAGC AGGTGCAGCC CAACTCGTTC AGCCTGGGCA 
     2521 TCTTCAACAG CAGGAAGGAG GACGAGGGCC AGTATGAATG CCATGTGACT GAATGGGTGC 
     2581 GGGCAGTGGA TGGCGAGTGG CAGATTGTTG GGGAGCGCCG GGCCAGCACT CCCATCTCCA 
     2641 TCACAGCTCT TGAAATGGGC TTCGCAGTCA CAGCCATCTC CCGGACACCG GGGGTGACCT 
     2701 ACAGCGACTC CTTTGACTTG CAGTGTATCA TCAAACCCCA CTACCCTGCC TGGGTCCCCG 
     2761 TGTCGGTGAC ATGGCGGTTC CAGCCGGTGG GCACGGTGGA GTTCCATGAC TTGGTGACCT 
     2821 TCACCCGGGA CGGAGGGGTC CAGTGGGGGG ACAGGTCCTC CAGCTTCCGA ACCCGAACTG 
     2881 CCATCGAGAA GGCTGAGTCC AGCAACAACG TCCGCCTAAG CATCAGCCGA GCCAGTGACA 
     2941 CGGAAGCAGG CAAGTACCAG TGTGTGGCAG AGCTGTGGCG GAAGAACTAC AACAACACCT 
     3001 GGACGCGACT GGCGGAGAGG ACCTCCAACC TGCTGGAGAT CAGGGTGCTG CAGCCAGTGA 
     3061 CAAAGCTGCA GGTGAGCAAA TCGAAGAGGA CCCTCACCCT GGTGGAAAAC AAGCCCATTC 
     3121 AGTTGAACTG CTCAGTCAAG TCTCAGACTA GCCAGAACTC CCACTTTGCG GTGCTCTGGT 
     3181 ATGTCCACAA GCCCTCGGAT GCCGATGGCA AGCTTATCCT GAAGACCACC CACAACTCCG 
     3241 CCTTTGAATA CGGTACTTAC GCCGAGGAGG AGGGCCTGAG AGCCAGGCTC CAGTTTGAGA 
     3301 GGCATGTGTC GGGGGGCCTG TTCAGCCTCA CCGTCCAGAG AGCCGAGGTC AGCGACAGCG 
     3361 GCAGCTACTA CTGCCACGTG GAGGAGTGGC TGCTGAGCCC CAACTACGCC TGGTACAAGC 
     3421 TGGCAGAGGA GGTTTCTGGG CGCACAGAAG TCACTGTGAA ACAGCCAGAC AGCCGCCTGA 
     3481 GGCTCAGCCA AGCCCAGGGG AACCTGTCGG TTCTGGAGAC CCGGCAGGTA CAGCTGGAGT 
     3541 GTGTGGTTCT CAACCGCACC AGCATAACCT CCCAGCTCAT GGTGGAATGG TTTGTATGGA 
     3601 AGCCCAACCA CCCTGAGCGG GAGACTGTGG CCCGCTTGAG CCGTGACGCC ACCTTCCACT 
     3661 ATGGAGAGCA GGCAGCCAAG AACAATCTGA AGGGGCGGCT GCATTTGGAG AGTCCTTCCC 
     3721 CCGGCGTGTA CCGTCTCTTC ATCCAGAACG TGGCTGTGCA GGACAGCGGG ACCTACAGCT 
     3781 GCCATGTGGA GGAGTGGCTG CCCAGCCCCA GTGGCATGTG GTATAAGCGG GCAGAGGACA 
     3841 CCGCTGGGCA GACAGCTCTG ACAGTCATGC GACCAGATGC TTCCCTGCAG GTGGACACAG 
     3901 TGGTCCCCAA TGCCACGGTC TCTGAGAAGG CAGCTTTCCA GCTGGACTGT AGCATCGTGT 
     3961 CCCGCTCCAG CCAGGACTCC CGCTTCGCTG TGGCCTGGTA TTCCCTGAGG ACTAAAGCTG 
     4021 GGGGGAAAAG GAGCAGCCCT GGCCTGGAAG AACAGGAAGA GGAAAGGGAG GAGGAGGAGG 
     4081 AGGAGGAGGA GGACGACGAC GACGACGACC CAACAGAGCG GACGGCCCTG CTGAGCGTGG 
     4141 GCCCAGATGC TGTCTTTGGC CCAGAGGGCA GTCCTTGGGA GGGCAGGCTT CGCTTCCAGA 
     4201 GGCTCTCCCC GGTGCTCTAC CGGCTCACAG TGCTGCAGGC AAGCCCCCAA GATACAGGCA 
     4261 ATTACTCCTG CCATGTGGAG GAGTGGCTGC CCAGCCCTCA GAAGGAATGG TACCGGCTGA 
     4321 CGGAGGAGGA GTCAGCCCCC ATCGGCATCC GTGTTCTAGA TACAAGTCCC ACCCTCCAGT 
     4381 CCATCATCTG CTCCAACGAC GCACTCTTCT ACTTCGTCTT CTTCTACCCT TTCCCCATCT 
     4441 TTGGCATTCT TATCATCACC ATCCTTCTGG TGCGTTTCAA GAGCCGGAAC TCCAGCAAGA 
     4501 ACTCTGATGG GAAGAATGGG GTGCCTCTGC TGTGGATCAA AGAGCCACAC CTCAACTACT 
     4561 CCCCTACTTG CCTGGAGCCC CCTGTTCTCA GTATCCATCC AGGGGCCATA GACACGCGTA 
     4621 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4681 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4741 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4801 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4861 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4921 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4981 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5041 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5101 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5161 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5221 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5281 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5341 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5401 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5461 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5521 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5581 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5641 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5701 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5761 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5821 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5881 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5941 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6001 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6061 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6121 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6181 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6241 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6301 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6361 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6421 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6481 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6541 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6601 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6661 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6721 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6781 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6841 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6901 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6961 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7021 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7081 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7141 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7201 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7261 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7321 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7381 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7441 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7501 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7561 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7621 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7681 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7741 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7801 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7861 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7921 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7981 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8041 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8101 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8161 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8221 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8281 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8341 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8401 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8461 AATTTT
//