LOCUS       RC221743                8337 bp    DNA     circular  SYN 
DEFINITION  ITGAM (Myc-DDK-tagged)-Human integrin, alpha M (complement component 3 receptor 3 subunit) (ITGAM), transcript variant 2
ACCESSION   NM_000632
VERSION     NM_000632.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4494..4585 
                     /label="Myc-DDK"  
     misc_feature    4615..5200 
                     /label="PolyA signal"  
     misc_feature    4672..4691 
                     /label="Restriction Enzyme"  
     misc_feature    5476..6148 
                     /label="ColE1"  
     misc_feature    6578..7372 
                     /label="Kan/Neo"  
     misc_feature    7407..7709 
                     /label="SV40 ori"  
     misc_feature    7795..8272 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4476..4482 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4476 
                     /label="ITGAM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCTCAGA GTCCTTCTGT TAACAGCCTT GACCTTATGT CATGGGTTCA 
     1081 ACTTGGACAC TGAAAACGCA ATGACCTTCC AAGAGAACGC AAGGGGCTTC GGGCAGAGCG 
     1141 TGGTCCAGCT TCAGGGATCC AGGGTGGTGG TTGGAGCCCC CCAGGAGATA GTGGCTGCCA 
     1201 ACCAAAGGGG CAGCCTCTAC CAGTGCGACT ACAGCACAGG CTCATGCGAG CCCATCCGCC 
     1261 TGCAGGTCCC CGTGGAGGCC GTGAACATGT CCCTGGGCCT GTCCCTGGCA GCCACCACCA 
     1321 GCCCCCCTCA GCTGCTGGCC TGTGGTCCCA CCGTGCACCA GACTTGCAGT GAGAACACGT 
     1381 ATGTGAAAGG GCTCTGCTTC CTGTTTGGAT CCAACCTACG GCAGCAGCCC CAGAAGTTCC 
     1441 CAGAGGCCCT CCGAGGGTGT CCTCAAGAGG ATAGTGACAT TGCCTTCTTG ATTGATGGCT 
     1501 CTGGTAGCAT CATCCCACAT GACTTTCGGC GGATGAAGGA GTTTGTCTCA ACTGTGATGG 
     1561 AGCAATTAAA AAAGTCCAAA ACCTTGTTCT CTTTGATGCA GTACTCTGAA GAATTCCGGA 
     1621 TTCACTTTAC CTTCAAAGAG TTCCAGAACA ACCCTAACCC AAGATCACTG GTGAAGCCAA 
     1681 TAACGCAGCT GCTTGGGCGG ACACACACGG CCACGGGCAT CCGCAAAGTG GTACGAGAGC 
     1741 TGTTTAACAT CACCAACGGA GCCCGAAAGA ATGCCTTTAA GATCCTAGTT GTCATCACGG 
     1801 ATGGAGAAAA GTTTGGCGAT CCCTTGGGAT ATGAGGATGT CATCCCTGAG GCAGACAGAG 
     1861 AGGGAGTCAT TCGCTACGTC ATTGGGGTGG GAGATGCCTT CCGCAGTGAG AAATCCCGCC 
     1921 AAGAGCTTAA TACCATCGCA TCCAAGCCGC CTCGTGATCA CGTGTTCCAG GTGAATAACT 
     1981 TTGAGGCTCT GAAGACCATT CAGAACCAGC TTCGGGAGAA GATCTTTGCG ATCGAGGGTA 
     2041 CTCAGACAGG AAGTAGCAGC TCCTTTGAGC ATGAGATGTC TCAGGAAGGC TTCAGCGCTG 
     2101 CCATCACCTC TAATGGCCCC TTGCTGAGCA CTGTGGGGAG CTATGACTGG GCTGGTGGAG 
     2161 TCTTTCTATA TACATCAAAG GAGAAAAGCA CCTTCATCAA CATGACCAGA GTGGATTCAG 
     2221 ACATGAATGA TGCTTACTTG GGTTATGCTG CCGCCATCAT CTTACGGAAC CGGGTGCAAA 
     2281 GCCTGGTTCT GGGGGCACCT CGATATCAGC ACATCGGCCT GGTAGCGATG TTCAGGCAGA 
     2341 ACACTGGCAT GTGGGAGTCC AACGCTAATG TCAAGGGCAC CCAGATCGGC GCCTACTTCG 
     2401 GGGCCTCCCT CTGCTCCGTG GACGTGGACA GCAACGGCAG CACCGACCTG GTCCTCATCG 
     2461 GGGCCCCCCA TTACTACGAG CAGACCCGAG GGGGCCAGGT GTCCGTGTGC CCCTTGCCCA 
     2521 GGGGGAGGGC TCGGTGGCAG TGTGATGCTG TTCTCTACGG GGAGCAGGGC CAACCCTGGG 
     2581 GCCGCTTTGG GGCAGCCCTA ACAGTGCTGG GGGACGTAAA TGGGGACAAG CTGACGGACG 
     2641 TGGCCATTGG GGCCCCAGGA GAGGAGGACA ACCGGGGTGC TGTTTACCTG TTTCACGGAA 
     2701 CCTCAGGATC TGGCATCAGC CCCTCCCATA GCCAGCGGAT AGCAGGCTCC AAGCTCTCTC 
     2761 CCAGGCTCCA GTATTTTGGT CAGTCACTGA GTGGGGGCCA GGACCTCACA ATGGATGGAC 
     2821 TGGTAGACCT GACTGTAGGA GCCCAGGGGC ACGTGCTGCT GCTCAGGTCC CAGCCAGTAC 
     2881 TGAGAGTCAA GGCAATCATG GAGTTCAATC CCAGGGAAGT GGCAAGGAAT GTATTTGAGT 
     2941 GTAATGATCA GGTGGTGAAA GGCAAGGAAG CCGGAGAGGT CAGAGTCTGC CTCCATGTCC 
     3001 AGAAGAGCAC ACGGGATCGG CTAAGAGAAG GACAGATCCA GAGTGTTGTG ACTTATGACC 
     3061 TGGCTCTGGA CTCCGGCCGC CCACATTCCC GCGCCGTCTT CAATGAGACA AAGAACAGCA 
     3121 CACGCAGACA GACACAGGTC TTGGGGCTGA CCCAGACTTG TGAGACCCTG AAACTACAGT 
     3181 TGCCGAATTG CATCGAGGAC CCAGTGAGCC CCATTGTGCT GCGCCTGAAC TTCTCTCTGG 
     3241 TGGGAACGCC ATTGTCTGCT TTCGGGAACC TCCGGCCAGT GCTGGCGGAG GATGCTCAGA 
     3301 GACTCTTCAC AGCCTTGTTT CCCTTTGAGA AGAATTGTGG CAATGACAAC ATCTGCCAGG 
     3361 ATGACCTCAG CATCACCTTC AGTTTCATGA GCCTGGACTG CCTCGTGGTG GGTGGGCCCC 
     3421 GGGAGTTCAA CGTGACAGTG ACTGTGAGAA ATGATGGTGA GGACTCCTAC AGGACACAGG 
     3481 TCACCTTCTT CTTCCCGCTT GACCTGTCCT ACCGGAAGGT GTCCACGCTC CAGAACCAGC 
     3541 GCTCACAGCG ATCCTGGCGC CTGGCCTGTG AGTCTGCCTC CTCCACCGAA GTGTCTGGGG 
     3601 CCTTGAAGAG CACCAGCTGC AGCATAAACC ACCCCATCTT CCCGGAAAAC TCAGAGGTCA 
     3661 CCTTTAATAT CACGTTTGAT GTAGACTCTA AGGCTTCCCT TGGAAACAAA CTGCTCCTCA 
     3721 AGGCCAATGT GACCAGTGAG AACAACATGC CCAGAACCAA CAAAACCGAA TTCCAACTGG 
     3781 AGCTGCCGGT GAAATATGCT GTCTACATGG TGGTCACCAG CCATGGGGTC TCCACTAAAT 
     3841 ATCTCAACTT CACGGCCTCA GAGAATACCA GTCGGGTCAT GCAGCATCAA TATCAGGTCA 
     3901 GCAACCTGGG GCAGAGGAGC CTCCCCATCA GCCTGGTGTT CTTGGTGCCC GTCCGGCTGA 
     3961 ACCAGACTGT CATATGGGAC CGCCCCCAGG TCACCTTCTC CGAGAACCTC TCGAGTACGT 
     4021 GCCACACCAA GGAGCGCTTG CCCTCTCACT CCGACTTTCT GGCTGAGCTT CGGAAGGCCC 
     4081 CCGTGGTGAA CTGCTCCATC GCTGTCTGCC AGAGAATCCA GTGTGACATC CCGTTCTTTG 
     4141 GCATCCAGGA AGAATTCAAT GCTACCCTCA AAGGCAACCT CTCGTTTGAC TGGTACATCA 
     4201 AGACCTCGCA TAACCACCTC CTGATCGTGA GCACAGCTGA GATCTTGTTT AACGATTCCG 
     4261 TGTTCACCCT GCTGCCGGGA CAGGGGGCGT TTGTGAGGTC CCAGACGGAG ACCAAAGTGG 
     4321 AGCCGTTCGA GGTCCCCAAC CCCCTGCCGC TCATCGTGGG CAGCTCTGTC GGGGGACTGC 
     4381 TGCTCCTGGC CCTCATCACC GCCGCGCTGT ACAAGCTCGG CTTCTTCAAG CGGCAATACA 
     4441 AGGACATGAT GAGTGAAGGG GGTCCCCCGG GGGCCGAACC CCAGACGCGT ACGCGGCCGC 
     4501 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4561 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4621 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4681 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4741 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4801 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4861 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4921 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4981 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5041 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5101 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5161 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5221 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5281 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5341 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5401 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5461 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5521 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5581 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5641 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5701 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5761 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5821 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5881 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5941 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6001 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6061 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6121 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6181 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6241 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6301 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6361 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6421 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6481 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6541 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6601 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6661 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6721 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6781 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6841 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6901 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6961 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7021 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7081 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7141 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7201 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7261 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7321 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7381 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7441 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7501 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7561 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7621 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7681 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7741 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7801 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7861 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7921 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7981 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8041 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8101 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8161 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8221 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8281 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//