LOCUS       RC221742                8634 bp    DNA     circular  SYN 
DEFINITION  REV1 (Myc-DDK-tagged)-Human REV1 homolog (S. cerevisiae) (REV1), transcript variant 1
ACCESSION   NM_016316
VERSION     NM_016316.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4791..4882 
                     /label="Myc-DDK"  
     misc_feature    4912..5497 
                     /label="PolyA signal"  
     misc_feature    4969..4988 
                     /label="Restriction Enzyme"  
     misc_feature    5773..6445 
                     /label="ColE1"  
     misc_feature    6875..7669 
                     /label="Kan/Neo"  
     misc_feature    7704..8006 
                     /label="SV40 ori"  
     misc_feature    8092..8569 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4773..4779 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4773 
                     /label="REV1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGCGAGGT GGATGGAGGA AGCGAGCTGA AAATGATGGC TGGGAAACAT 
     1081 GGGGTGGGTA TATGGCTGCC AAGGTCCAGA AATTGGAGGA ACAGTTTCGA TCAGATGCTG 
     1141 CTATGCAGAA GGATGGGACT TCATCTACAA TTTTTAGTGG AGTTGCCATC TATGTTAATG 
     1201 GATACACAGA TCCTTCCGCT GAGGAATTGA GAAAACTAAT GATGTTGCAT GGAGGTCAAT 
     1261 ACCATGTATA TTATTCCAGA TCTAAAACAA CACATATTAT TGCCACAAAT CTTCCCAATG 
     1321 CCAAAATTAA AGAATTAAAG GGGGAAAAAG TAATTCGACC AGAATGGATT GTGGAAAGCA 
     1381 TCAAAGCTGG ACGACTCCTC TCCTACATTC CATATCAGCT GTACACCAAG CAGTCCAGTG 
     1441 TGCAGAAAGG TCTCAGCTTT AATCCTGTAT GCAGACCTGA GGATCCTCTG CCAGGTCCAA 
     1501 GCAATATAGC CAAACAGCTC AACAACAGGG TAAATCACAT CGTTAAGAAG ATTGAAACGG 
     1561 AAAATGAAGT CAAAGTCAAT GGCATGAACA GTTGGAATGA AGAAGATGAA AATAATGATT 
     1621 TTAGTTTTGT GGATCTGGAG CAGACCTCTC CGGGAAGGAA ACAGAATGGA ATTCCGCATC 
     1681 CCAGAGGGAG CACTGCCATT TTTAATGGAC ACACTCCTAG CTCTAATGGT GCCTTAAAGA 
     1741 CACAGGATTG CTTGGTGCCC ATGGTCAACA GTGTTGCCAG CAGGCTTTCT CCAGCCTTTT 
     1801 CCCAGGAGGA GGATAAGGCT GAGAAGAGCA GCACTGATTT CAGAGACTGC ACTCTGCAGC 
     1861 AGTTGCAGCA AAGCACCAGA AACACAGATG CTTTGCGGAA TCCACACAGA ACTAATTCTT 
     1921 TCTCATTATC ACCTTTGCAC AGTAACACTA AAATCAATGG TGCTCACCAC TCCACTGTTC 
     1981 AGGGGCCTTC AAGCACAAAA AGCACTTCTT CAGTATCTAC GTTTAGCAAG GCAGCACCTT 
     2041 CAGTGCCATC CAAACCTTCA GACTGCAATT TTATTTCAAA CTTCTATTCT CATTCAAGAC 
     2101 TGCATCACAT ATCAATGTGG AAGTGTGAAT TGACTGAGTT TGTCAATACC CTACAAAGAC 
     2161 AAAGTAATGG TATCTTTCCA GGAAGGGAAA AGTTAAAAAA AATGAAAACA GGCAGGTCTG 
     2221 CACTTGTTGT AACTGACACA GGAGATATGT CAGTATTGAA TTCTCCCAGA CATCAGAGCT 
     2281 GTATAATGCA TGTTGATATG GATTGCTTCT TTGTATCAGT GGGTATACGA AATAGACCAG 
     2341 ATCTCAAAGG AAAACCAGTG GCTGTTACAA GTAACAGAGG CACAGGAAGG GCACCTTTAC 
     2401 GTCCTGGCGC TAACCCCCAG CTGGAGTGGC AGTATTACCA GAATAAAATC CTGAAAGGCA 
     2461 AAGCAGCAGA TATACCAGAT TCATCATTGT GGGAGAATCC AGATTCTGCG CAAGCAAATG 
     2521 GAATTGATTC TGTTTTGTCA AGGGCTGAAA TTGCATCTTG TAGTTATGAG GCCAGGCAAC 
     2581 TTGGCATTAA GAACGGAATG TTTTTTGGGC ATGCTAAACA ACTATGTCCT AATCTTCAAG 
     2641 CTGTTCCATA CGATTTTCAT GCATATAAGG AAGTCGCACA AACATTGTAT GAAACATTGG 
     2701 CAAGCTACAC TCATAACATT GAAGCTGTCA GTTGTGATGA AGCGCTGGTA GACATTACCG 
     2761 AAATCCTTGC AGAGACCAAA CTTACTCCTG ATGAATTTGC AAATGCTGTT CGTATGGAAA 
     2821 TCAAAGACCA GACGAAATGT GCTGCCTCTG TTGGAATTGG TTCTAATATT CTCCTGGCTA 
     2881 GAATGGCAAC TAGAAAAGCA AAACCAGATG GGCAGTACCA CCTAAAACCA GAAGAAGTAG 
     2941 ATGATTTTAT CAGAGGCCAG CTAGTGACCA ATCTACCAGG AGTTGGACAT TCAATGGAAT 
     3001 CTAAGTTGGC ATCTTTGGGA ATTAAAACTT GTGGAGACTT GCAGTATATG ACCATGGCAA 
     3061 AACTCCAAAA AGAATTTGGT CCCAAAACAG GTCAGATGCT TTATAGGTTC TGCCGTGGCT 
     3121 TGGATGATAG ACCAGTTCGA ACTGAAAAGG AAAGAAAATC TGTTTCAGCT GAGATCAACT 
     3181 ATGGAATAAG GTTTACTCAG CCAAAAGAGG CAGAAGCTTT TCTTCTGAGT CTTTCAGAAG 
     3241 AAATTCAAAG AAGACTAGAA GCCACTGGCA TGAAGGGTAA ACGTCTAACT CTCAAAATCA 
     3301 TGGTACGAAA GCCTGGGGCT CCTGTAGAAA CTGCAAAATT TGGAGGCCAT GGAATTTGTG 
     3361 ATAACATTGC CAGGACTGTA ACTCTTGACC AGGCAACAGA TAATGCAAAA ATAATTGGAA 
     3421 AGGCGATGCT AAACATGTTT CATACAATGA AACTAAATAT ATCAGATATG AGAGGGGTTG 
     3481 GGATTCACGT GAATCAGTTG GTTCCAACTA ATCTGAACCC TTCCACATGT CCCAGTCGCC 
     3541 CATCAGTTCA GTCAAGCCAC TTTCCTAGTG GGTCATACTC TGTCCGTGAT GTCTTCCAAG 
     3601 TTCAGAAAGC TAAGAAATCC ACCGAAGAGG AGCACAAAGA AGTATTTCGG GCTGCTGTGG 
     3661 ATCTGGAAAT ATCATCTGCT TCTAGAACTT GCACTTTCTT GCCACCTTTT CCTGCACATC 
     3721 TGCCGACCAG TCCTGATACT AACAAGGCTG AGTCTTCAGG GAAATGGAAT GGTCTACATA 
     3781 CTCCTGTCAG TGTGCAGTCG AGACTTAACC TGAGTATAGA GGTCCCGTCA CCTTCCCAGC 
     3841 TGGATCAGTC TGTTTTAGAA GCACTTCCAC CTGATCTCCG GGAACAAGTA GAGCAAGTCT 
     3901 GTGCTGTCCA GCAAGCAGAG TCACATGGCG ACAAAAAGAA AGAACCAGTA AATGGCTGTA 
     3961 ATACAGGAAT TTTGCCACAA CCAGTTGGGA CAGTCTTGTT GCAAATACCA GAACCTCAAG 
     4021 AATCGAACAG TGACGCAGGA ATAAATTTAA TAGCCCTTCC AGCATTTTCA CAGGTGGACC 
     4081 CTGAGGTATT TGCTGCCCTT CCTGCTGAAC TTCAGAGGGA GCTGAAAGCA GCGTATGATC 
     4141 AAAGACAAAG GCAGGGCGAG AACAGCACTC ACCAGCAGTC AGCCAGCGCA TCTGTGCCAA 
     4201 AGAATCCTTT ACTTCATCTA AAGGCAGCAG TGAAAGAAAA GAAAAGAAAC AAGAAGAAAA 
     4261 AAACCATTGG TTCACCAAAA AGGATTCAGA GTCCTTTGAA TAACAAGCTG CTTAACAGTC 
     4321 CTGCAAAAAC TCTGCCAGGG GCCTGTGGCA GTCCCCAGAA GTTAATTGAT GGGTTTCTAA 
     4381 AACATGAAGG ACCTCCTGCA GAGAAACCCC TGGAAGAACT CTCTGCTTCT ACTTCAGGTG 
     4441 TGCCAGGCCT TTCTAGTTTG CAGTCTGACC CAGCTGGCTG TGTGAGACCT CCAGCACCCA 
     4501 ATCTAGCTGG AGCTGTTGAA TTCAATGATG TGAAGACCTT GCTCAGAGAA TGGATAACTA 
     4561 CAATTTCAGA TCCAATGGAA GAAGACATTC TCCAAGTTGT GAAATACTGT ACTGATCTAA 
     4621 TAGAAGAAAA AGATTTGGAA AAACTGGATC TAGTTATAAA ATACATGAAA AGGCTGATGC 
     4681 AGCAATCGGT GGAATCGGTT TGGAATATGG CATTTGACTT TATTCTTGAC AATGTCCAGG 
     4741 TGGTTTTACA ACAAACTTAT GGAAGCACAT TAAAAGTTAC AACGCGTACG CGGCCGCTCG 
     4801 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4861 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4921 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4981 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5041 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5101 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5161 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5221 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5281 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5341 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5401 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5461 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5521 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5581 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5641 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5701 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5761 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5821 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5881 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5941 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6001 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6061 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6121 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6181 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6241 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6301 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6361 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6421 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6481 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6541 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6601 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6661 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6721 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6781 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6841 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6901 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6961 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7021 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7081 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7141 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7201 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7261 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7321 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7381 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7441 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7501 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7561 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7621 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7681 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7741 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7801 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7861 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7921 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7981 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8041 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8101 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8161 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8221 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8281 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8341 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8401 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8461 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8521 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8581 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//