LOCUS       RC221729                8655 bp    DNA     circular  SYN 
DEFINITION  NEK1 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)-related kinase 1 (NEK1), transcript variant 2
ACCESSION   NM_012224
VERSION     NM_012224.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4812..4903 
                     /label="Myc-DDK"  
     misc_feature    4933..5518 
                     /label="PolyA signal"  
     misc_feature    4990..5009 
                     /label="Restriction Enzyme"  
     misc_feature    5794..6466 
                     /label="ColE1"  
     misc_feature    6896..7690 
                     /label="Kan/Neo"  
     misc_feature    7725..8027 
                     /label="SV40 ori"  
     misc_feature    8113..8590 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4794..4800 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4794 
                     /label="NEK1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAAGTAT GTTAGACTAC AGAAGATTGG AGAAGGTTCA TTTGGAAAAG 
     1081 CCATTCTTGT TAAATCTACA GAAGATGGCA GACAGTATGT TATCAAGGAA ATTAACATCT 
     1141 CAAGAATGTC CAGTAAAGAA AGAGAAGAAT CAAGGAGAGA AGTTGCAGTA TTGGCAAACA 
     1201 TGAAGCATCC AAATATTGTC CAGTATAGAG AATCATTTGA AGAAAATGGC TCTCTCTACA 
     1261 TAGTAATGGA TTACTGTGAG GGAGGGGATC TGTTTAAGCG AATAAATGCT CAGAAAGGCG 
     1321 TTTTGTTTCA AGAGGATCAG ATTTTGGACT GGTTTGTACA GATATGTTTG GCCCTGAAAC 
     1381 ATGTACATGA TAGAAAAATT CTTCATCGAG ACATTAAATC TCAGAACATA TTTTTAACTA 
     1441 AAGATGGAAC AGTACAACTT GGAGATTTTG GAATTGCTAG AGTTCTTAAT AGTACTGTAG 
     1501 AGCTGGCTCG AACTTGCATA GGGACCCCAT ACTACTTGTC ACCTGAAATC TGTGAAAACA 
     1561 AACCTTACAA TAATAAAAGT GACATTTGGG CTCTGGGGTG TGTCCTTTAT GAGCTGTGTA 
     1621 CACTTAAACA TGCTTTTGAA GCTGGCAGTA TGAAAAACCT GGTACTGAAG ATAATATCTG 
     1681 GATCTTTTCC ACCTGTGTCT TTGCATTATT CCTATGATCT CCGCAGTTTG GTGTCTCAGT 
     1741 TATTTAAAAG AAATCCTAGG GATAGACCAT CAGTCAACTC CATATTGGAG AAAGGTTTTA 
     1801 TAGCCAAACG CATTGAAAAG TTTCTCTCTC CTCAGCTTAT TGCAGAAGAA TTTTGTCTAA 
     1861 AAACATTTTC GAAGTTTGGA TCACAGCCTA TACCAGCTAA AAGACCAGCT TCAGGACAAA 
     1921 ACTCGATTTC TGTTATGCCT GCTCAGAAAA TTACAAAGCC TGCCGCTAAA TATGGAATAC 
     1981 CTTTAGCATA TAAGAAATAT GGAGATAAAA AATTACACGA AAAGAAACCA CTGCAAAAAC 
     2041 ATAAACAGGC CCATCAAACT CCAGAGAAGA GAGTGAATAC TGGAGAAGAA AGGAGGAAAA 
     2101 TATCTGAGGA AGCAGCAAGA AAGAGAAGGC TGGAATTTAT TGAAAAAGAA AAGAAACAAA 
     2161 AGGATCAGAT TATTAGTTTA ATGAAGGCTG AACAAATGAA AAGGCAAGAA AAGGAAAGGT 
     2221 TGGAAAGAAT AAATAGGGCC AGGGAACAAG GATGGAGAAA TGTGCTAAGT GCTGGTGGAA 
     2281 GTGGTGAAGT AAAGGCTCCT TTTCTGGGCA GTGGAGGGAC TATAGCTCCA TCATCTTTTT 
     2341 CTTCTCGAGG ACAGTATGAA CATTACCATG CCATTTTTGA CCAAATGCAG CAACAAAGAG 
     2401 CAGAAGATAA TGAAGCTAAA TGGAAAAGAG AAATATATGG TCGAGGTCTT CCAGAAAGAG 
     2461 GAATTCTGCC TGGAGTTCGT CCAGGATTTC CTTATGGGGC TGCAGGTCAT CACCATTTTC 
     2521 CTGATGCTGA TGATATTAGA AAAACTTTGA AAAGATTGAA GGCGGTGTCT AAACAAGCCA 
     2581 ATGCAAACAG GCAAAAAGGG CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC 
     2641 TGCAGCGAAA ACGGGAAGCT ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGTTTATC 
     2701 TGGCAAGACT GAGGCAAATA AGACTACAGA ATTTCAATGA GCGCCAACAG ATTAAAGCCA 
     2761 AACTTCGTGG TGAAAAGAAA GAAGCTAATC ATTCTGAAGG ACAAGAAGGA AGTGAAGAGG 
     2821 CTGACATGAG GCGCAAAAAA ATCGAATCAC TGAAGGCCCA TGCAAATGCA CGTGCTGCTG 
     2881 TACTAAAAGA ACAACTAGAA CGAAAGAGAA AGGAGGCTTA TGAGAGAGAA AAAAAAGTGT 
     2941 GGGAAGAGCA TTTGGTGGCT AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA CCTTTGGGAC 
     3001 AGCATGAAAC AGGTGGCTCT CCATCAAAGC AACAGATGAG ATCTGTTATT TCTGTAACTT 
     3061 CAGCTTTGAA AGAAGTTGGC GTGGACAGTA GTTTAACTGA TACCCGGGAA ACTTCAGAAG 
     3121 AGATGCAAAA GACCAACAAT GCTATTTCAA GTAAGCGAGA AATACTTCGT AGATTAAATG 
     3181 AAAATCTTAA AGCTCAAGAA GATGAAAAAG GAAAGCAGAA TCTCTCTGAT ACTTTTGAGA 
     3241 TAAATGTTCA TGAAGATGCC AAAGAGCATG AAAAAGAAAA ATCAGTTTCA TCTGATCGCA 
     3301 AGAAGTGGGA GGCAGGAGGT CAACTTGTGA TTCCTCTGGA TGAGTTAACA CTAGATACAT 
     3361 CCTTCTCTAC AACTGAAAGA CATACAGTGG GAGAAGTTAT TAAATTAGGT CCTAATGGAT 
     3421 CTCCAAGAAG AGCCTGGGGG AAAAGTCCGA CAGATTCTGT TCTAAAGATA CTTGGAGAAG 
     3481 CTGAACTACA ACTTCAGACA GAACTATTAG AAAATACAAC TATTAGAAGT GAGATTTCTC 
     3541 CCGAAGGGGA AAAGTACAAA CCCTTAATTA CTGGAGAAAA AAAAGTACAA TGTATTTCAC 
     3601 ATGAAATAAA CCCATCAGCT ATTGTTGATT CTCCTGTTGA GACAAAAAGT CCCGAGTTCA 
     3661 GTGAGGCATC TCCACAGATG TCATTGAAAC TGGAAGGAAA TTTAGAAGAA CCTGATGATT 
     3721 TGGAAACAGA AATTCTACAA GAGCCAAGTG GAACAAACAA AGATGAGAGC TTGCCATGCA 
     3781 CTATTACTGA TGTGTGGATT AGTGAGGAAA AAGAAACAAA GGAAACTCAG TCGGCAGATA 
     3841 GGATCACCAT TCAGGAAAAT GAAGTTTCTG AAGATGGAGT CTCGAGTACT GTGGACCAAC 
     3901 TTAGTGACAT TCATATAGAG CCTGGAACCA ATGATTCTCA GCACTCTAAA TGTGATGTAG 
     3961 ATAAGTCTGT GCAACCGGAA CCATTTTTCC ATAAGGTGGT TCATTCTGAA CACTTGAACT 
     4021 TAGTCCCTCA AGTTCAATCA GTTCAGTGTT CACCAGAAGA ATCCTTTGCA TTTCGATCTC 
     4081 ACTCGCATTT ACCACCAAAA AATAAAAACA AGAATTCCTT GCTGATTGGA CTTTCAACTG 
     4141 GTCTGTTTGA TGCAAACAAC CCAAAGATGT TAAGGACATG TTCACTTCCA GATCTCTCAA 
     4201 AGCTGTTCAG AACCCTTATG GATGTTCCCA CCGTAGGAGA TGTTCGTCAA GACAATCTTG 
     4261 AAATAGATGA AATTGAAGAT GAAAACATTA AAGAAGGACC TTCTGATTCT GAAGACATTG 
     4321 TGTTTGAAGA AACTGACACA GATTTACAAG AGCTGCAGGC CTCGATGGAA CAGTTACTTA 
     4381 GGGAACAACC TGGTGAAGAA TACAGTGAAG AAGAAGAGTC AGTCTTGAAG AACAGTGATG 
     4441 TGGAGCCAAC TGCAAATGGG ACAGATGTGG CAGATGAAGA TGACAATCCC AGCAGTGAAA 
     4501 GTGCCCTGAA CGAAGAATGG CACTCAGATA ACAGTGATGG TGAAATTGCT AGTGAATGTG 
     4561 AATGCGATAG TGTCTTTAAC CATTTAGAGG AACTGAGACT TCATCTGGAG CAGGAAATGG 
     4621 GCTTTGAAAA ATTCTTTGAG GTTTATGAGA AAATAAAGGC TATTCATGAA GATGAAGATG 
     4681 AAAATATTGA AATTTGTTCA AAAATAGTTC AAAATATTTT GGGAAATGAA CATCAGCATC 
     4741 TTTATGCCAA GATTCTTCAT TTAGTCATGG CAGATGGAGC CTACCAAGAA GATAATGATG 
     4801 AAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4861 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4921 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4981 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5041 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5101 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5161 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5221 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5281 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5341 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5401 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5461 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5521 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5581 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5641 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5701 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5761 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5821 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5881 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5941 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6001 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6061 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6121 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6181 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6241 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6301 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6361 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6421 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6481 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6541 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6601 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6661 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6721 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6781 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6841 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6901 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6961 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7021 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7081 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7141 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7201 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7261 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7321 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7381 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7441 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7501 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7561 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7621 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7681 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7741 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7801 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7861 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7921 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7981 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8041 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8101 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8161 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8221 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8281 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8341 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8401 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8461 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8521 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8581 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8641 TTTAACGCGA ATTTT
//