LOCUS       RC221710                8610 bp    DNA     circular  SYN 
DEFINITION  ATP2B2 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 2 (ATP2B2), transcript variant 1
ACCESSION   NM_001001331
VERSION     NM_001001331.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4767..4858 
                     /label="Myc-DDK"  
     misc_feature    4888..5473 
                     /label="PolyA signal"  
     misc_feature    4945..4964 
                     /label="Restriction Enzyme"  
     misc_feature    5749..6421 
                     /label="ColE1"  
     misc_feature    6851..7645 
                     /label="Kan/Neo"  
     misc_feature    7680..7982 
                     /label="SV40 ori"  
     misc_feature    8068..8545 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4749..4755 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4749 
                     /label="ATP2B2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTGACATG ACCAACAGCG ACTTTTACTC CAAAAACCAA AGAAATGAGT 
     1081 CGAGCCATGG GGGCGAGTTC GGGTGCACAA TGGAGGAGCT CCGCTCCCTC ATGGAGCTGC 
     1141 GGGGCACTGA GGCTGTGGTC AAGATCAAGG AGACTTATGG GGACACCGAA GCCATCTGCC 
     1201 GGCGCCTCAA AACCTCACCT GTTGAAGGTT TGCCGGGCAC CGCTCCAGAC CTGGAAAAGA 
     1261 GAAAGCAAAT TTTTGGGCAA AACTTTATAC CTCCAAAGAA GCCAAAAACC TTCCTGCAGC 
     1321 TCGTGTGGGA GGCGCTGCAG GACGTGACGC TCATCATCCT GGAGATTGCC GCCATCATCT 
     1381 CCCTGGGGCT GTCCTTCTAC CACCCGCCCG GCGAGGGCAA CGAAGGATGT GCGACGGCCC 
     1441 AGGGTGGGGC AGAGGATGAA GGAGAGGCAG AGGCAGGTTG GATCGAGGGG GCCGCCATTC 
     1501 TCCTCTCAGT TATCTGTGTG GTCCTGGTCA CGGCCTTCAA TGACTGGAGC AAAGAGAAAC 
     1561 AGTTCCGGGG CCTGCAGAGC CGCATCGAGC AGGAACAGAA ATTTACCGTG GTCCGGGCTG 
     1621 GCCAGGTGGT CCAGATCCCT GTGGCTGAGA TCGTGGTTGG GGACATAGCC CAGGTCAAAT 
     1681 ATGGTGACCT CCTCCCTGCC GACGGCCTCT TCATCCAGGG CAATGACCTC AAGATTGATG 
     1741 AAAGCTCCCT AACTGGAGAG TCTGACCAGG TGCGCAAGTC CGTGGACAAG GACCCCATGC 
     1801 TGCTGTCAGG AACCCACGTG ATGGAGGGCT CAGGACGGAT GTTGGTGACT GCTGTGGGTG 
     1861 TGAACTCTCA GACTGGCATC ATCTTTACCC TCCTGGGGGC TGGTGGTGAA GAGGAAGAGA 
     1921 AGAAAGACAA AAAAGGTGTG AAGAAGGGGG ATGGCCTTCA GCTACCAGCA GCAGACGGTG 
     1981 CGGCAGCTTC AAATGCTGCA GATAGTGCGA ATGCCAGCCT AGTCAATGGT AAAATGCAGG 
     2041 ATGGCAATGT GGACGCCAGC CAGAGCAAAG CCAAACAACA GGACGGGGCA GCCGCCATGG 
     2101 AGATGCAGCC CCTCAAGAGT GCCGAGGGCG GCGACGCTGA CGACAGGAAG AAGGCCAGCA 
     2161 TGCACAAGAA GGAGAAGTCC GTGCTGCAGG GCAAGCTCAC CAAGCTGGCT GTGCAGATCG 
     2221 GGAAGGCGGG CTTGGTGATG TCAGCCATCA CGGTGATCAT CCTGGTGCTC TACTTCACTG 
     2281 TGGACACCTT CGTGGTCAAC AAGAAGCCGT GGCTGCCTGA GTGCACGCCC GTCTACGTGC 
     2341 AGTACTTTGT CAAGTTCTTC ATCATTGGCG TGACGGTGCT GGTGGTCGCC GTGCCCGAGG 
     2401 GGCTCCCTCT GGCCGTCACC ATCTCGTTGG CCTATTCGGT GAAGAAAATG ATGAAGGACA 
     2461 ACAACCTGGT ACGCCACCTG GATGCCTGTG AGACCATGGG CAATGCCACA GCCATCTGCT 
     2521 CAGACAAGAC AGGCACGCTG ACCACCAATC GCATGACAGT GGTACAGGCC TATGTCGGCG 
     2581 ACGTCCACTA TAAAGAGATC CCCGACCCCA GCTCCATCAA CACCAAGACC ATGGAGCTGC 
     2641 TGATCAATGC CATCGCCATC AACAGCGCCT ACACCACCAA GATTCTGCCC CCAGAGAAGG 
     2701 AGGGCGCCCT GCCTCGGCAG GTGGGCAACA AGACGGAGTG CGGCCTGCTG GGCTTCGTGC 
     2761 TGGACCTGAA GCAGGACTAC GAGCCCGTGC GCAGCCAGAT GCCAGAGGAG AAGTTGTACA 
     2821 AAGTGTACAC CTTCAACTCC GTGCGCAAGT CCATGAGCAC TGTCATCAAG CTGCCCGACG 
     2881 AGAGCTTCCG CATGTACAGC AAGGGGGCTT CTGAGATCGT GCTCAAGAAG TGCTGCAAAA 
     2941 TCCTCAATGG GGCGGGAGAG CCTCGTGTCT TCCGGCCCCG CGACCGGGAC GAGATGGTAA 
     3001 AGAAGGTGAT TGAGCCCATG GCTTGCGATG GGCTCCGCAC TATCTGCGTG GCCTACCGCG 
     3061 ACTTCCCCAG CAGCCCGGAG CCGGACTGGG ACAATGAGAA TGACATCCTC AACGAACTCA 
     3121 CCTGCATCTG CGTGGTGGGC ATCGAGGACC CGGTGCGGCC AGAGGTCCCA GAAGCCATCC 
     3181 GCAAGTGCCA GCGGGCAGGC ATCACGGTCC GCATGGTCAC TGGCGACAAT ATCAACACGG 
     3241 CTCGGGCCAT CGCCATCAAG TGTGGCATCA TCCATCCTGG GGAGGACTTT CTGTGCCTCG 
     3301 AGGGCAAGGA GTTCAACAGG AGGATCCGCA ACGAGAAGGG GGAGATTGAG CAGGAGCGAA 
     3361 TTGACAAGAT CTGGCCAAAG CTGCGGGTGC TGGCTCGCTC CTCCCCAACG GACAAGCATA 
     3421 CCCTGGTTAA AGGCATCATC GACAGCACAC ACACTGAGCA GCGGCAGGTG GTGGCCGTGA 
     3481 CGGGGGACGG GACCAACGAC GGGCCTGCAC TCAAGAAGGC CGACGTGGGC TTCGCCATGG 
     3541 GCATCGCAGG CACTGACGTG GCCAAGGAGG CCTCAGACAT CATCCTGACA GACGACAATT 
     3601 TCAGCAGCAT CGTCAAGGCA GTGATGTGGG GCCGCAACGT CTATGACAGC ATCTCCAAAT 
     3661 TCTTGCAGTT CCAGCTCACC GTCAACGTGG TGGCCGTGAT TGTGGCCTTC ACAGGCGCCT 
     3721 GCATCACGCA GGACTCCCCT CTGAAGGCCG TGCAGATGCT CTGGGTGAAC CTCATCATGG 
     3781 ACACGTTTGC CTCGCTGGCA CTGGCCACTG AGCCGCCCAC GGAGACCCTG CTGCTGAGGA 
     3841 AGCCGTACGG CCGCAACAAG CCGCTCATCT CCAGGACCAT GATGAAGAAC ATCCTGGGCC 
     3901 ATGCTGTCTA CCAGCTTGCC CTCATCTTCA CCCTGCTCTT TGTTGGCGAG AAGATGTTCC 
     3961 AGATCGACAG CGGGAGGAAC GCGCCCCTGC ATTCGCCACC CTCAGAACAT TACACCATCA 
     4021 TCTTCAACAC CTTCGTCATG ATGCAGCTCT TCAACGAGAT CAACGCCCGC AAGATCCACG 
     4081 GCGAGCGCAA TGTCTTTGAC GGCATCTTCC GGAACCCCAT CTTCTGCACC ATCGTGCTGG 
     4141 GCACCTTTGC CATCCAGATA GTGATCGTGC AGTTTGGAGG GAAGCCATTC AGCTGCTCTC 
     4201 CACTGCAGCT GGACCAGTGG ATGTGGTGCA TATTCATTGG GTTAGGAGAG CTCGTTTGGG 
     4261 GCCAGGTCAT CGCCACCATC CCGACCAGCA GACTCAAGTT CCTCAAGGAG GCAGGCAGGC 
     4321 TCACACAGAA GGAGGAGATC CCGGAGGAGG AGCTCAACGA GGACGTGGAG GAGATCGACC 
     4381 ACGCGGAGCG GGAGCTGCGG CGGGGCCAGA TCCTGTGGTT CCGAGGCCTG AATCGGATCC 
     4441 AGACACAGAT CCGCGTCGTG AAGGCGTTCC GTAGCTCTCT CTATGAAGGT TTAGAAAAGC 
     4501 CTGAATCTCG AACCTCCATC CATAACTTCA TGGCTCATCC TGAATTCCGG ATCGAAGATT 
     4561 CCCAGCCCCA CATCCCCCTC ATTGATGACA CCGACCTGGA AGAAGATGCC GCGCTCAAGC 
     4621 AGAACTCGAG CCCGCCGTCA TCCCTCAACA AGAACAACAG CGCCATCGAC AGTGGGATCA 
     4681 ACCTGACGAC CGACACAAGC AAATCAGCTA CCTCTTCAAG TCCAGGGAGC CCCATCCACA 
     4741 GCCTGGAGAC GTCGCTTACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4801 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4861 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4921 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4981 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5041 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5101 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5161 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5221 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5281 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5341 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5401 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5461 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5521 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5581 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5641 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5701 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5761 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5821 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5881 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5941 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6001 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6061 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6121 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6181 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6241 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6301 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6361 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6421 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6481 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6541 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6601 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6661 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6721 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6781 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6841 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6901 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6961 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7021 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7081 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7141 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7201 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7261 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7321 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7381 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7441 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7501 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7561 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7621 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7681 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7741 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7801 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7861 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7921 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7981 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8041 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8101 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8161 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8221 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8281 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8341 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8401 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8461 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8521 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8581 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//