LOCUS       RC221685                8103 bp    DNA     circular  SYN 
DEFINITION  SEMA5A (Myc-DDK-tagged)-Human sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A)
ACCESSION   NM_003966
VERSION     NM_003966.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4260..4351 
                     /label="Myc-DDK"  
     misc_feature    4381..4966 
                     /label="PolyA signal"  
     misc_feature    4438..4457 
                     /label="Restriction Enzyme"  
     misc_feature    5242..5914 
                     /label="ColE1"  
     misc_feature    6344..7138 
                     /label="Kan/Neo"  
     misc_feature    7173..7475 
                     /label="SV40 ori"  
     misc_feature    7561..8038 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4242..4248 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4242 
                     /label="SEMA5A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGGAACC TGTGTTATAG CATGGCTGTT CTCAAGCCTG GGGCTGTGGA 
     1081 GACTCGCCCA CCCAGAGGCC CAGGGTACGA CTCAGTGCCA GAGAACCGAG CATCCAGTCA 
     1141 TCTCCTATAA AGAAATTGGC CCCTGGTTAC GGGAGTTCAG AGCGAAGAAT GCTGCGGATT 
     1201 TCTCGCAGTT AACATTTGAC CCAGGACAGA AAGAACTTGT TGTAGGAGCA AGAAACTACC 
     1261 TCTTCAGGTT ACAGCTTGAG GATCTGTCTC TTATCCAGGC TGTGGAATGG GAGTGTGATG 
     1321 AAGCTACCAA AAAGGCCTGT TACAGCAAAG GCAAATCAAA GGAGGAATGT CAGAACTACA 
     1381 TCCGGGTGCT TCTGGTGGGT GGCGACCGGT TATTCACCTG TGGGACCAAT GCATTCACGC 
     1441 CTGTCTGCAC CAACCGCTCG TTGAGCAACC TGGCTGAGAT CCATGATCAG ATCAGTGGCA 
     1501 TGGCCCGCTG TCCCTACAGT CCCCAGCACA ATTCCACAGC GCTCCTCACA GCTGGTGGGG 
     1561 AGCTCTATGC TGCTACAGCC ATGGATTTTC CAGGACGTGA TCCTGCCATT TACCGAAGCC 
     1621 TAGGCATTTT ACCTCCTCTC CGCACGGCGC AGTACAACTC CAAATGGCTC AATGAGCCAA 
     1681 ACTTTGTGTC ATCTTATGAC ATCGGAAATT TTACCTACTT CTTTTTCCGA GAAAATGCAG 
     1741 TAGAGCATGA CTGTGGGAAA ACAGTGTTCT CCAGAGCTGC CCGGGTGTGC AAGAACGATA 
     1801 TTGGTGGGCG CTTCCTGCTG GAAGACACCT GGACCACATT CATGAAGGCT CGCCTGAACT 
     1861 GCTCCCGTCC TGGGGAAGTC CCCTTTTACT ACAACGAATT GCAGAGTACT TTCTTCCTGC 
     1921 CTGAGCTGGA TTTGATCTAT GGCATCTTTA CCACCAATGT GAACAGCATT GCGGCCTCAG 
     1981 CTGTGTGCGT CTTCAACCTG AGCGCCATCG CGCAGGCCTT CTCTGGGCCC TTCAAGTACC 
     2041 AAGAAAACTC GCGCTCGGCC TGGCTACCGT ATCCCAACCC AAACCCCCAC TTCCAGTGTG 
     2101 GCACCGTGGA CCAGGGCCTG TACGTGAACC TGACCGAGAG AAATCTGCAG GATGCTCAGA 
     2161 AGTTCATTCT GGTGCATGAG GTGGTACAGC CAGTGACCAC AGTGCCCTCC TTCATGGAGG 
     2221 ACAATAGCCG CTTTTCCCAC GTGGCAGTCG ACGTGGTGCA GGGCAGAGAA GCGCTCGTCC 
     2281 ACATCATCTA TTTGGCCACA GATTACGGAA CCATTAAGAA AGTGCGGGTA CCCCTGAATC 
     2341 AGACCTCAAG CAGCTGTTTG CTGGAAGAGA TTGAGCTCTT CCCTGAGAGG CGGAGGGAGC 
     2401 CCATCAGGAG CCTGCAGATC CTGCACAGCC AGAGTGTCCT GTTCGTGGGG CTGCGGGAGC 
     2461 ACGTGGTCAA GATCCCCCTG AAGAGGTGCC AGTTCTACCG CACACGCAGC ACCTGCATTG 
     2521 GGGCCCAGGA CCCTTACTGT GGCTGGGATG TGGTAATGAA GAAATGCACA AGCCTGGAGG 
     2581 AGAGCCTGAG CATGACGCAG TGGGAACAGA GCATCTCTGC GTGTCCGACC AGGAATCTCA 
     2641 CCGTGGATGG GCACTTTGGT GTGTGGTCTC CGTGGACGCC TTGCACGCAC ACAGATGGCA 
     2701 GCGCCGTGGG ATCCTGCCTC TGTCGAACCC GCTCCTGCGA CAGCCCGGCC CCGCAGTGTG 
     2761 GTGGCTGGCA GTGCGAGGGC CCTGGCATGG AGATCGCCAA CTGTTCCAGG AACGGAGGCT 
     2821 GGACTCCCTG GACCTCGTGG TCTCCCTGCA GCACTACCTG TGGGATCGGC TTCCAGGTGC 
     2881 GGCAGCGCTC CTGCAGCAAC CCCACTCCCA GGCACGGGGG CCGGGTGTGC GTGGGACAGA 
     2941 ACCGCGAGGA AAGATACTGC AATGAACATT TGCTATGTCC CCCACACATG TTCTGGACAG 
     3001 GCTGGGGTCC TTGGGAACGG TGCACAGCCC AATGCGGGGG TGGCATTCAA GCTCGCCGCA 
     3061 GGATCTGTGA GAATGGGCCT GACTGTGCAG GCTGCAATGT GGAGTACCAG TCTTGCAACA 
     3121 CCAACCCGTG TCCTGAGCTG AAGAAGACCA CGCCCTGGAC ACCCTGGACA CCTGTCAACA 
     3181 TCTCTGACAA CGGCGACCAC TATGAGCAAC GATTCCGATA CACATGCAAA GCCCGCCTGG 
     3241 CTGATCCGAA TTTGCTGGAA GTGGGAAGAC AGAGAATCGA AATGCGGTAC TGTTCTAGCG 
     3301 ACGGCACCAG TGGCTGCTCC ACAGATGGGC TTTCTGGGGA TTTCCTGCGT GCTGGGAGAT 
     3361 ACTCTGCCCA CACGGTCAAC GGGGCTTGGT CAGCCTGGAC GTCGTGGTCA CAGTGCAGCC 
     3421 GTGACTGCAG CAGGGGCATT CGGAACCGGA AGCGTGTTTG CAACAACCCC GAACCCAAGT 
     3481 ATGGGGGAAT GCCTTGCCTT GGCCCATCTC TGGAATACCA GGAATGCAAC ACTTTGCCCT 
     3541 GCCCAGTGGA TGGCGTGTGG TCTTGCTGGT CCCCCTGGAC AAAATGTTCA GCAACATGCG 
     3601 GCGGTGGACA CTATATGAGG ACCCGCTCTT GCTCCAATCC AGCCCCGGCC TATGGAGGGG 
     3661 ACATCTGCCT GGGGCTGCAC ACAGAAGAGG CACTCTGCAA CACGCAGCCC TGCCCAGAGA 
     3721 GCTGGTCGGA GTGGTCGGAC TGGTCTGAGT GTGAAGCCTC TGGCGTCCAA GTCCGCGCCC 
     3781 GCCAGTGCAT CCTCCTGTTC CCCATGGGCA GCCAGTGCTC CGGGAACACC ACGGAGAGCC 
     3841 GGCCGTGTGT GTTTGACTCT AATTTCATCC CAGAAGTATC TGTGGCAAGA TCCAGTAGCG 
     3901 TAGAAGAGAA AAGGTGTGGA GAGTTCAACA TGTTCCACAT GATCGCCGTG GGGCTGAGCA 
     3961 GCTCCATCCT CGGCTGCCTC CTCACCCTGC TCGTCTATAC TTACTGCCAG CGGTACCAGC 
     4021 AGCAATCCCA CGATGCGACT GTCATCCACC CCGTCTCACC TGCCCCCCTT AATACCAGCA 
     4081 TAACCAACCA CATCAACAAA CTGGACAAGT ACGACTCGGT GGAGGCCATC AAGGCATTTA 
     4141 ACAAAAACAA CTTGATCCTA GAGGAAAGAA ACAAATACTT CAACCCACAT CTCACTGGGA 
     4201 AGACCTATTC TAATGCCTAC TTTACAGATC TCAATAATTA TGATGAGTAC ACGCGTACGC 
     4261 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4321 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4381 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4441 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4501 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4561 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4621 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4681 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4741 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4801 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4861 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4921 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4981 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5041 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5101 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5161 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5221 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5281 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5341 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5401 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5461 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5521 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5581 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5641 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5701 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5761 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5821 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5881 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5941 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6001 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6061 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6121 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6181 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6241 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6301 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6361 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6421 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6481 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6541 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6601 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6661 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6721 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6781 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6841 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6901 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6961 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7021 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7081 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7141 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7201 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7261 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7321 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7381 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7441 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7501 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7561 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7621 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7681 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7741 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7801 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7861 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7921 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7981 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8041 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8101 TTT
//