LOCUS       RC221682                8409 bp    DNA     circular  SYN 
DEFINITION  LARS (Myc-DDK-tagged)-Human leucyl-tRNA synthetase (LARS)
ACCESSION   NM_020117
VERSION     NM_020117.8
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4566..4657 
                     /label="Myc-DDK"  
     misc_feature    4687..5272 
                     /label="PolyA signal"  
     misc_feature    4744..4763 
                     /label="Restriction Enzyme"  
     misc_feature    5548..6220 
                     /label="ColE1"  
     misc_feature    6650..7444 
                     /label="Kan/Neo"  
     misc_feature    7479..7781 
                     /label="SV40 ori"  
     misc_feature    7867..8344 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4548..4554 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4548 
                     /label="LARS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAAAGA AAAGGAACAG CCAAAGTGGA CTTTTTGAAG AAGATTGAGA 
     1081 AAGAAATCCA ACAGAAATGG GATACTGAGA GAGTGTTTGA GGTCAATGCA TCTAATTTAG 
     1141 AGAAACAGAC CAGCAAGGGC AAGTATTTTG TAACCTTCCC ATATCCATAT ATGAATGGAC 
     1201 GCCTTCATTT GGGACACACG TTTTCTTTAT CCAAATGTGA GTTTGCTGTA GGGTACCAGC 
     1261 GATTGAAAGG AAAATGTTGT CTGTTTCCCT TTGGCCTGCA CTGTACTGGA ATGCCTATTA 
     1321 AGGCATGTGC TGATAAGTTG AAAAGAGAAA TAGAGCTGTA TGGTTGCCCC CCTGATTTTC 
     1381 CAGATGAAGA AGAGGAAGAG GAAGAAACCA GTGTTAAAAC AGAAGATATA ATAATTAAGG 
     1441 ATAAAGCTAA AGGAAAAAAG AGTAAAGCTG CTGCTAAAGC TGGATCTTCT AAATACCAGT 
     1501 GGGGCATTAT GAAATCCCTT GGCCTGTCTG ATGAAGAGAT AGTAAAATTT TCTGAAGCAG 
     1561 AACATTGGCT TGATTATTTC CCGCCACTGG CTATTCAGGA TTTAAAAAGA ATGGGTTTGA 
     1621 AGGTAGACTG GCGTCGTTCC TTCATCACCA CTGATGTTAA TCCTTACTAT GATTCATTTG 
     1681 TCAGATGGCA ATTTTTAACA TTAAGAGAAA GAAACAAAAT TAAATTTGGG AAGCGGTATA 
     1741 CAATTTACTC TCCGAAAGAT GGACAGCCTT GCATGGATCA TGATAGACAA ACTGGAGAGG 
     1801 GTGTTGGACC TCAGGAATAT ACTTTACTCA AATTGAAGGC GCTTGAGCCA TACCCATCTA 
     1861 AATTAAGTGG CCTGAAAGGT AAAAATATTT TCTTGGTGGC TGCTACTCTC AGACCTGAGA 
     1921 CCATGTTTGG GCAGACAAAT TGTTGGGTTC GTCCTGATAT GAAGTACATT GGATTTGAGA 
     1981 CGGTGAATGG TGATATATTC ATCTGTACCC AAAAAGCAGC CAGGAATATG TCATACCAGG 
     2041 GCTTTACCAA AGACAATGGC GTGGTGCCTG TTGTTAAGGA ATTAATGGGG GAGGAAATTC 
     2101 TTGGTGCATC ACTTTCTGCA CCTTTAACAT CATACAAGGT GATCTATGTT CTCCCAATGC 
     2161 TAACTATTAA GGAGGATAAA GGCACTGGTG TGGTTACAAG TGTTCCTTCC GACTCCCCTG 
     2221 ATGATATTGC TGCCCTCAGA GACTTGAAGA AAAAGCAAGC CTTACGAGCA AAATATGGAA 
     2281 TTAGAGATGA CATGGTCTTG CCATTTGAGC CGGTGCCAGT CATTGAAATC CCAGGTTTTG 
     2341 GAAATCTTTC TGCTGTAACC ATTTGTGATG AGTTGAAAAT TCAGAGCCAG AATGACCGGG 
     2401 AAAAACTTGC AGAAGCAAAG GAGAAGATAT ATCTAAAAGG ATTTTATGAG GGTATCATGT 
     2461 TGGTGGATGG ATTTAAAGGA CAGAAGGTTC AAGATGTAAA GAAGACTATT CAGAAAAAGA 
     2521 TGATTGACGC TGGAGATGCA CTTATTTACA TGGAACCAGA GAAACAAGTG ATGTCCAGGT 
     2581 CGTCAGATGA ATGTGTTGTG GCTCTGTGTG ACCAGTGGTA CTTGGATTAT GGAGAAGAGA 
     2641 ATTGGAAGAA ACAGACATCT CAGTGCTTGA AGAACCTGGA AACATTCTGT GAGGAGACCA 
     2701 GGAGGAATTT TGAAGCCACC TTAGGTTGGC TACAAGAACA TGCTTGCTCA AGAACTTATG 
     2761 GTCTAGGCAC TCACCTGCCT TGGGATGAGC AGTGGCTGAT TGAATCACTT TCTGACTCCA 
     2821 CTATTTACAT GGCATTTTAC ACAGTTGCAC ACCTATTGCA GGGGGGTAAC TTGCATGGAC 
     2881 AGGCAGAGTC TCCGCTGGGC ATTAGACCGC AACAGATGAC CAAGGAAGTT TGGGATTATG 
     2941 TTTTCTTCAA GGAGGCTCCA TTTCCTAAGA CTCAGATTGC AAAGGAAAAA TTAGATCAGT 
     3001 TAAAGCAGGA GTTTGAATTC TGGTATCCTG TTGATCTTCG CGTCTCTGGC AAGGATCTTG 
     3061 TTCCAAATCA TCTTTCATAT TACCTTTATA ATCATGTGGC TATGTGGCCG GAACAAAGTG 
     3121 ATAAATGGCC TACAGCTGTG AGAGCAAATG GACATCTCCT CCTGAACTCT GAGAAGATGT 
     3181 CAAAATCCAC AGGCAACTTC CTCACTTTGA CCCAAGCTAT TGACAAATTT TCAGCAGATG 
     3241 GAATGCGTTT GGCTCTGGCT GATGCTGGTG ACACTGTAGA AGATGCCAAC TTTGTGGAAG 
     3301 CCATGGCAGA TGCAGGTATT CTCCGTCTGT ACACCTGGGT AGAGTGGGTG AAAGAAATGG 
     3361 TTGCCAACTG GGACAGCCTA AGAAGTGGTC CTGCCAGCAC TTTCAATGAT AGAGTTTTTG 
     3421 CCAGTGAATT GAATGCAGGA ATTATAAAAA CAGATCAAAA CTATGAAAAG ATGATGTTTA 
     3481 AAGAAGCTTT GAAAACAGGG TTTTTTGAGT TTCAGGCCGC AAAAGATAAG TACCGTGAAT 
     3541 TGGCTGTGGA AGGGATGCAC AGAGAACTTG TGTTCCGGTT TATTGAAGTT CAGACACTTC 
     3601 TCCTCGCTCC ATTCTGTCCA CATTTGTGTG AGCACATCTG GACACTCCTG GGAAAGCCTG 
     3661 ACTCAATTAT GAATGCTTCA TGGCCTGTGG CAGGTCCTGT GGATGAAGTT TTAATACACT 
     3721 CCTCACAGTA TCTTATGGAA GTAACACATG ACCTTAGACT ACGACTCAAG AACTATATGA 
     3781 TGCCAGCTAA AGGGAAGAAG ACCGACAAAC AACCCCTGCA GAAGCCCTCA CATTGCACCA 
     3841 TCTATGTGGC AAAGAACTAT CCACCTTGGC AACATACCAC CCTGTCTGTT CTACGTAAAC 
     3901 ACTTTGAGGC CAATAACGGA AAACTGCCTG ACAACAAAGT CATTGCTAGT GAACTAGGCA 
     3961 GTATGCCAGA ACTGAAGAAA TACATGAAGA AAGTCATGCC ATTTGTTGCC ATGATTAAGG 
     4021 AAAATCTGGA GAAGATGGGG CCTCGTATTC TGGATTTGCA ATTAGAATTT GATGAAAAGG 
     4081 CTGTGCTTAT GGAGAATATA GTCTATCTGA CTAATTCGCT TGAGCTAGAA CACATAGAAG 
     4141 TCAAGTTTGC CTCCGAAGCA GAAGATAAAA TCAGGGAAGA CTGCTGTCCT GGGAAACCAC 
     4201 TTAATGTTTT TAGAATAGAA CCTGGTGTGT CCGTTTCTCT GGTGAATCCC CAGCCATCCA 
     4261 ATGGCCACTT CTCAACCAAA ATTGAAATCA AGCAAGGAGA TAACTGTGAT TCCATAATCA 
     4321 GGCGTTTAAT GAAAATGAAT CGAGGAATTA AAGACCTTTC CAAAGTGAAA CTGATGAGAT 
     4381 TTGATGATCC ACTGTTGGGG CCTCGACGAG TTCCTGTCCT GGGAAAGGAG TACACCGAGA 
     4441 AGACCCCCAT TTCTGAGCAT GCTGTTTTCA ATGTGGACCT CATGAGCAAG AAAATTCATC 
     4501 TGACTGAGAA TGGGATAAGG GTGGATATTG GCGATACAAT AATCTATCTG GTTCATACGC 
     4561 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4621 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4681 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4741 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4801 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4861 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4921 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4981 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5041 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5101 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5161 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5221 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5281 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5341 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5401 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5461 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5521 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5581 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5641 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5701 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5761 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5821 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5881 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5941 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6001 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6061 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6121 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6181 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6241 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6301 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6361 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6421 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6481 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6541 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6601 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6661 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6721 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6781 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6841 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6901 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6961 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7021 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7081 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7141 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7201 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7261 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7321 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7381 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7441 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7501 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7561 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7621 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7681 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7741 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7801 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7861 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7921 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7981 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8041 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8101 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8161 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8221 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8281 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8341 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8401 GCGAATTTT
//