LOCUS       RC221634                9246 bp    DNA     circular  SYN 
DEFINITION  FANCA (Myc-DDK-tagged)-Human Fanconi anemia, complementation group A (FANCA), transcript variant 1
ACCESSION   NM_000135
VERSION     NM_000135.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5403..5494 
                     /label="Myc-DDK"  
     misc_feature    5524..6109 
                     /label="PolyA signal"  
     misc_feature    5581..5600 
                     /label="Restriction Enzyme"  
     misc_feature    6385..7057 
                     /label="ColE1"  
     misc_feature    7487..8281 
                     /label="Kan/Neo"  
     misc_feature    8316..8618 
                     /label="SV40 ori"  
     misc_feature    8704..9181 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5385..5391 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5385 
                     /label="FANCA"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCGACTCG TGGGTCCCGA ACTCCGCCTC GGGCCAGGAC CCAGGGGGCC 
     1081 GCCGGAGGGC CTGGGCCGAG CTGCTGGCGG GAAGGGTCAA GAGGGAAAAA TATAATCCTG 
     1141 AAAGGGCACA GAAATTAAAG GAATCAGCTG TGCGCCTCCT GCGAAGCCAT CAGGACCTGA 
     1201 ATGCCCTTTT GCTTGAGGTA GAAGGTCCAC TGTGTAAAAA ATTGTCTCTC AGCAAAGTGA 
     1261 TTGACTGTGA CAGTTCTGAG GCCTATGCTA ATCATTCTAG TTCATTTATA GGCTCTGCTT 
     1321 TGCAGGATCA AGCCTCAAGG CTGGGGGTTC CCGTGGGTAT TCTCTCAGCC GGGATGGTTG 
     1381 CCTCTAGCGT GGGACAGATC TGCACGGCTC CAGCGGAGAC CAGTCACCCT GTGCTGCTGA 
     1441 CTGTGGAGCA GAGAAAGAAG CTGTCTTCCC TGTTAGAGTT TGCTCAGTAT TTATTGGCAC 
     1501 ACAGTATGTT CTCCCGTCTT TCCTTCTGTC AAGAATTATG GAAAATACAG AGTTCTTTGT 
     1561 TGCTTGAAGC GGTGTGGCAT CTTCACGTAC AAGGCATTGT GAGCCTGCAA GAGCTGCTGG 
     1621 AAAGCCATCC CGACATGCAT GCTGTGGGAT CGTGGCTCTT CAGGAATCTG TGCTGCCTTT 
     1681 GTGAACAGAT GGAAGCATCC TGCCAGCATG CTGACGTCGC CAGGGCCATG CTTTCTGATT 
     1741 TTGTTCAAAT GTTTGTTTTG AGGGGATTTC AGAAAAACTC AGATCTGAGA AGAACTGTGG 
     1801 AGCCTGAAAA AATGCCGCAG GTCACGGTTG ATGTACTGCA GAGAATGCTG ATTTTTGCAC 
     1861 TTGACGCTTT GGCTGCTGGA GTACAGGAGG AGTCCTCCAC TCACAAGATC GTGAGGTGCT 
     1921 GGTTCGGAGT GTTCAGTGGA CACACGCTTG GCAGTGTAAT TTCCACAGAT CCTCTGAAGA 
     1981 GGTTCTTCAG TCATACCCTG ACTCAGATAC TCACTCACAG CCCTGTGCTG AAAGCATCTG 
     2041 ATGCTGTTCA GATGCAGAGA GAGTGGAGCT TTGCGCGGAC ACACCCTCTG CTCACCTCAC 
     2101 TGTACCGCAG GCTCTTTGTG ATGCTGAGTG CAGAGGAGTT GGTTGGCCAT TTGCAAGAAG 
     2161 TTCTGGAAAC GCAGGAGGTT CACTGGCAGA GAGTGCTCTC CTTTGTGTCT GCCCTGGTTG 
     2221 TCTGCTTTCC AGAAGCGCAG CAGCTGCTTG AAGACTGGGT GGCGCGTTTG ATGGCCCAGG 
     2281 CATTCGAGAG CTGCCAGCTG GACAGCATGG TCACTGCGTT CCTGGTTGTG CGCCAGGCAG 
     2341 CACTGGAGGG CCCCTCTGCG TTCCTGTCAT ATGCAGACTG GTTCAAGGCC TCCTTTGGGA 
     2401 GCACACGAGG CTACCATGGC TGCAGCAAGA AGGCCCTGGT CTTCCTGTTT ACGTTCTTGT 
     2461 CAGAACTCGT GCCTTTTGAG TCTCCCCGGT ACCTGCAGGT GCACATTCTC CACCCACCCC 
     2521 TGGTTCCCGG CAAGTACCGC TCCCTCCTCA CAGACTACAT CTCATTGGCC AAGACACGGC 
     2581 TGGCCGACCT CAAGGTTTCT ATAGAAAACA TGGGACTCTA CGAGGATTTG TCATCAGCTG 
     2641 GGGACATTAC TGAGCCCCAC AGCCAAGCTC TTCAGGATGT TGAAAAGGCC ATCATGGTGT 
     2701 TTGAGCATAC GGGGAACATC CCAGTCACCG TCATGGAGGC CAGCATATTC AGGAGGCCTT 
     2761 ACTACGTGTC CCACTTCCTC CCCGCCCTGC TCACACCTCG AGTGCTCCCC AAAGTCCCTG 
     2821 ACTCCCGTGT GGCGTTTATA GAGTCTCTGA AGAGAGCAGA TAAAATCCCC CCATCTCTGT 
     2881 ACTCCACCTA CTGCCAGGCC TGCTCTGCTG CTGAAGAGAA GCCAGAAGAT GCAGCCCTGG 
     2941 GAGTGAGGGC AGAACCCAAC TCTGCTGAGG AGCCCCTGGG ACAGCTCACA GCTGCACTGG 
     3001 GAGAGCTGAG AGCCTCCATG ACAGACCCCA GCCAGCGTGA TGTTATATCG GCACAGGTGG 
     3061 CAGTGATTTC TGAAAGACTG AGGGCTGTCC TGGGCCACAA TGAGGATGAC AGCAGCGTTG 
     3121 AGATATCAAA GATTCAGCTC AGCATCAACA CGCCGAGACT GGAGCCACGG GAACACATGG 
     3181 CTGTGGACCT CCTGCTGACG TCTTTCTGTC AGAACCTGAT GGCTGCCTCC AGTGTCGCTC 
     3241 CCCCGGAGAG GCAGGGTCCC TGGGCTGCCC TCTTCGTGAG GACCATGTGT GGACGTGTGC 
     3301 TCCCTGCAGT GCTCACCCGG CTCTGCCAGC TGCTCCGTCA CCAGGGCCCG AGCCTGAGTG 
     3361 CCCCACATGT GCTGGGGTTG GCTGCCCTGG CCGTGCACCT GGGTGAGTCC AGGTCTGCGC 
     3421 TCCCAGAGGT GGATGTGGGT CCTCCTGCAC CTGGTGCTGG CCTTCCTGTC CCTGCGCTCT 
     3481 TTGACAGCCT CCTGACCTGT AGGACGAGGG ATTCCTTGTT CTTCTGCCTG AAATTTTGTA 
     3541 CAGCAGCAAT TTCTTACTCT CTCTGCAAGT TTTCTTCCCA GTCACGAGAT ACTTTGTGCA 
     3601 GCTGCTTATC TCCAGGCCTT ATTAAAAAGT TTCAGTTCCT CATGTTCAGA TTGTTCTCAG 
     3661 AGGCCCGACA GCCTCTTTCT GAGGAGGACG TAGCCAGCCT TTCCTGGAGA CCCTTGCACC 
     3721 TTCCTTCTGC AGACTGGCAG AGAGCTGCCC TCTCTCTCTG GACACACAGA ACCTTCCGAG 
     3781 AGGTGTTGAA AGAGGAAGAT GTTCACTTAA CTTACCAAGA CTGGTTACAC CTGGAGCTGG 
     3841 AAATTCAACC TGAAGCTGAT GCTCTTTCAG ATACTGAACG GCAGGACTTC CACCAGTGGG 
     3901 CGATCCATGA GCACTTTCTC CCTGAGTCCT CGGCTTCAGG GGGCTGTGAC GGAGACCTGC 
     3961 AGGCTGCGTG TACCATTCTT GTCAACGCAC TGATGGATTT CCACCAAAGC TCAAGGAGTT 
     4021 ATGACCACTC AGAAAATTCT GATTTGGTCT TTGGTGGCCG CACAGGAAAT GAGGATATTA 
     4081 TTTCCAGATT GCAGGAGATG GTAGCTGACC TGGAGCTGCA GCAAGACCTC ATAGTGCCTC 
     4141 TCGGCCACAC CCCTTCCCAG GAGCACTTCC TCTTTGAGAT TTTCCGCAGA CGGCTCCAGG 
     4201 CTCTGACAAG CGGGTGGAGC GTGGCTGCCA GCCTTCAGAG ACAGAGGGAG CTGCTAATGT 
     4261 ACAAACGGAT CCTCCTCCGC CTGCCTTCGT CTGTCCTCTG CGGCAGCAGC TTCCAGGCAG 
     4321 AACAGCCCAT CACTGCCAGA TGCGAGCAGT TCTTCCACTT GGTCAACTCT GAGATGAGAA 
     4381 ACTTCTGCTC CCACGGAGGT GCCCTGACAC AGGACATCAC TGCCCACTTC TTCAGGGGCC 
     4441 TCCTGAACGC CTGTCTGCGG AGCAGAGACC CCTCCCTGAT GGTCGACTTC ATACTGGCCA 
     4501 AGTGCCAGAC GAAATGCCCC TTAATTTTGA CCTCTGCTCT GGTGTGGTGG CCGAGCCTGG 
     4561 AGCCTGTGCT GCTCTGCCGG TGGAGGAGAC ACTGCCAGAG CCCGCTGCCC CGGGAACTGC 
     4621 AGAAGCTACA AGAAGGCCGG CAGTTTGCCA GCGATTTCCT CTCCCCTGAG GCTGCCTCCC 
     4681 CAGCACCCAA CCCGGACTGG CTCTCAGCTG CTGCACTGCA CTTTGCGATT CAACAAGTCA 
     4741 GGGAAGAAAA CATCAGGAAG CAGCTAAAGA AGCTGGACTG CGAGAGAGAG GAGCTATTGG 
     4801 TTTTCCTTTT CTTCTTCTCC TTGATGGGCC TGCTGTCGTC ACATCTGACC TCAAATAGCA 
     4861 CCACAGACCT GCCAAAGGCT TTCCACGTTT GTGCAGCAAT CCTCGAGTGT TTAGAGAAGA 
     4921 GGAAGATATC CTGGCTGGCA CTCTTTCAGT TGACAGAGAG TGACCTCAGG CTGGGGCGGC 
     4981 TCCTCCTCCG TGTGGCCCCG GATCAGCACA CCAGGCTGCT GCCTTTCGCT TTTTACAGTC 
     5041 TTCTCTCCTA CTTCCATGAA GACGCGGCCA TCAGGGAAGA GGCCTTCCTG CATGTTGCTG 
     5101 TGGACATGTA CTTGAAGCTG GTCCAGCTCT TCGTGGCTGG GGATACAAGC ACAGTTTCAC 
     5161 CTCCAGCTGG CAGGAGCCTG GAGCTCAAGG GTCAGGGCAA CCCCGTGGAA CTGATAACAA 
     5221 AAGCTCGTCT TTTTCTGCTG CAGTTAATAC CTCGGTGCCC GAAAAAGAGC TTCTCACACG 
     5281 TGGCAGAGCT GCTGGCTGAT CGTGGGGACT GCGACCCAGA GGTGAGCGCC GCCCTCCAGA 
     5341 GCAGACAGCA GGCTGCCCCT GACGCTGACC TGTCCCAGGA GCCTCATCTC TTCACGCGTA 
     5401 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5461 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5521 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5581 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5641 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5701 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5761 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5821 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5881 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5941 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     6001 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     6061 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     6121 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     6181 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     6241 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     6301 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6361 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6421 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6481 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6541 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6601 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6661 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6721 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6781 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6841 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6901 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6961 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     7021 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     7081 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     7141 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     7201 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     7261 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     7321 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7381 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7441 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7501 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7561 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7621 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7681 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7741 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7801 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7861 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7921 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7981 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     8041 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     8101 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     8161 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     8221 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     8281 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     8341 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8401 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8461 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8521 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8581 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8641 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8701 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8761 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8821 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8881 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8941 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     9001 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     9061 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     9121 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     9181 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     9241 AATTTT
//