LOCUS       RC221617                8553 bp    DNA     circular  SYN 
DEFINITION  ADAMTS16 (Myc-DDK-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 16 (ADAMTS16)
ACCESSION   NM_139056
VERSION     NM_139056.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4710..4801 
                     /label="Myc-DDK"  
     misc_feature    4831..5416 
                     /label="PolyA signal"  
     misc_feature    4888..4907 
                     /label="Restriction Enzyme"  
     misc_feature    5692..6364 
                     /label="ColE1"  
     misc_feature    6794..7588 
                     /label="Kan/Neo"  
     misc_feature    7623..7925 
                     /label="SV40 ori"  
     misc_feature    8011..8488 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4692..4698 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4692 
                     /label="ADAMTS16"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGCCCCGC GCGCGCGGAT GGCGGGGCTT GGCGGCGCTG TGGATGCTGT 
     1081 TGGCGCAGGT GGCCGAGCAG GCACCTGCGT GCGCCATGGG ACCCGCAGCG GCAGCGCCTG 
     1141 GGAGCCCGAG CGTCCCGCGT CCTCCTCCAC CCGCGGAGCG GCCGGGCTGG ATGGAAAAGG 
     1201 GCGAATATGA CCTGGTCTCT GCCTACGAGG TTGACCACAG GGGCGATTAC GTGTCCCATG 
     1261 AAATCATGCA CCATCAGCGG CGGAGAAGAG CAGTGCCCGT GTCCGAGGTT GAGTCTCTTC 
     1321 ACCTTCGGCT GAAAGGCTCC AGGCACGACT TCCACATGGA TCTGAGGACT TCCAGCAGCC 
     1381 TAGTGGCTCC TGGCTTTATT GTGCAGACGT TGGGAAAGAC AGGCACTAAG TCTGTGCAGA 
     1441 CTTTACCGCC AGAGGACTTC TGTTTCTATC AAGGCTCTTT GCGATCACAC AGAAACTCCT 
     1501 CAGTGGCCCT TTCAACCTGC CAAGGCTTGT CAGGCATGAT ACGAACAGAA GAGGCAGATT 
     1561 ACTTCCTAAG GCCACTTCCT TCACACCTCT CATGGAAACT CGGCAGAGCT GCCCAAGGCA 
     1621 GCTCGCCATC CCACGTACTG TACAAGAGAT CCACAGAGCC CCATGCTCCT GGGGCCAGTG 
     1681 AGGTCCTGGT GACCTCAAGG ACATGGGAGC TGGCACATCA ACCCCTGCAC AGCAGCGACC 
     1741 TTCGCCTGGG ACTGCCACAA AAGCAGCATT TCTGTGGAAG ACGCAAGAAA TACATGCCCC 
     1801 AGCCTCCCAA GGAAGACCTC TTCATCTTGC CAGATGAGTA TAAGTCTTGC TTACGGCATA 
     1861 AGCGCTCTCT TCTGAGGTCC CATAGAAATG AAGAACTGAA CGTGGAGACC TTGGTGGTGG 
     1921 TCGACAAAAA GATGATGCAA AACCATGGCC ATGAAAATAT CACCACCTAC GTGCTCACGA 
     1981 TACTCAACAT GGTATCTGCT TTATTCAAAG ATGGAACAAT AGGAGGAAAC ATCAACATTG 
     2041 CAATTGTAGG TCTGATTCTT CTAGAAGATG AACAGCCAGG ACTGGTGATA AGTCACCACG 
     2101 CAGACCACAC CTTAAGTAGC TTCTGCCAGT GGCAGTCTGG ATTGATGGGG AAAGATGGGA 
     2161 CTCGTCATGA CCACGCCATC TTACTGACTG GTCTGGATAT ATGTTCCTGG AAGAATGAGC 
     2221 CCTGTGACAC TTTGGGATTT GCACCCATAA GTGGAATGTG TAGTAAATAT CGCAGCTGCA 
     2281 CGATTAATGA AGATACAGGT CTTGGACTGG CCTTCACCAT TGCCCATGAG TCTGGACACA 
     2341 ACTTTGGCAT GATTCATGAT GGAGAAGGGA ACATGTGCAA AAAGTCCGAG GGCAACATCA 
     2401 TGTCCCCTAC ATTGGCAGGA CGCAATGGAG TCTTCTCCTG GTCACCCTGC AGCCGCCAGT 
     2461 ATCTACACAA ATTTCTAAGC ACCGCTCAAG CTATCTGCCT TGCTGATCAG CCAAAGCCTG 
     2521 TGAAGGAATA CAAGTATCCT GAGAAATTGC CAGGAGAATT ATATGATGCA AACACACAGT 
     2581 GCAAGTGGCA GTTCGGAGAG AAAGCCAAGC TCTGCATGCT GGACTTTAAA AAGGACATCT 
     2641 GTAAAGCCCT GTGGTGCCAT CGTATTGGAA GGAAATGTGA GACTAAATTT ATGCCAGCAG 
     2701 CAGAAGGCAC AATTTGTGGG CATGACATGT GGTGCCGGGG AGGACAGTGT GTGAAATATG 
     2761 GTGATGAAGG CCCCAAGCCC ACCCATGGCC ACTGGTCGGA CTGGTCTTCT TGGTCCCCAT 
     2821 GCTCCAGGAC CTGCGGAGGG GGAGTATCTC ATAGGAGTCG CCTCTGCACC AACCCCAAGC 
     2881 CATCGCATGG AGGGAAGTTC TGTGAGGGCT CCACTCGCAC TCTGAAGCTC TGCAACAGTC 
     2941 AGAAATGTCC CCGGGACAGT GTTGACTTCC GTGCTGCTCA GTGTGCCGAG CACAACAGCA 
     3001 GACGATTCAG AGGGCGGCAC TACAAGTGGA AGCCTTACAC TCAAGTAGAA GATCAGGACT 
     3061 TATGCAAACT CTACTGTATC GCAGAAGGAT TTGATTTCTT CTTTTCTTTG TCAAATAAAG 
     3121 TCAAAGATGG GACTCCATGC TCGGAGGATA GCCGTAATGT TTGTATAGAT GGGATATGTG 
     3181 AGAGAGTTGG ATGTGACAAT GTCCTTGGAT CTGATGCTGT TGAAGACGTC TGTGGGGTGT 
     3241 GTAACGGGAA TAACTCAGCC TGCACGATTC ACAGGGGTCT CTACACCAAG CACCACCACA 
     3301 CCAACCAGTA TTATCACATG GTCACCATTC CTTCTGGAGC CCGGAGTATC CGCATCTATG 
     3361 AAATGAACGT CTCTACCTCC TACATTTCTG TGCGCAATGC CCTCAGAAGG TACTACCTGA 
     3421 ATGGGCACTG GACCGTGGAC TGGCCCGGCC GGTACAAATT TTCGGGCACT ACTTTCGACT 
     3481 ACAGACGGTC CTATAATGAG CCCGAGAACT TAATCGCTAC TGGACCAACC AACGAGACAC 
     3541 TGATTGTGGA GCTGCTGTTT CAGGGAAGGA ACCCGGGTGT TGCCTGGGAA TACTCCATGC 
     3601 CTCGCTTGGG GACCGAGAAG CAGCCCCCTG CCCAGCCCAG CTACACTTGG GCCATCGTGC 
     3661 GCTCTGAGTG CTCCGTGTCC TGCGGAGGGG GACAGATGAC CGTGAGAGAG GGCTGCTACA 
     3721 GAGACCTGAA GTTTCAAGTA AATATGTCCT TCTGCAATCC CAAGACACGA CCTGTCACGG 
     3781 GGCTGGTGCC TTGCAAAGTA TCTGCCTGTC CTCCCAGCTG GTCCGTGGGG AACTGGAGTG 
     3841 CCTGCAGTCG GACGTGTGGC GGGGGTGCCC AGAGCCGCCC CGTGCAGTGC ACACGGCGGG 
     3901 TGCACTATGA CTCGGAGCCA GTCCCGGCCA GCCTGTGCCC TCAGCCTGCT CCCTCCAGCA 
     3961 GGCAGGCCTG CAACTCTCAG AGCTGCCCAC CTGCATGGAG CGCCGGGCCC TGGGCAGAGT 
     4021 GCTCACACAC CTGTGGGAAG GGGTGGAGGA AGCGGGCAGT GGCCTGTAAG AGCACCAACC 
     4081 CCTCGGCCAG AGCGCAGCTG CTGCCCGACG CTGTCTGCAC CTCCGAGCCC AAGCCCAGGA 
     4141 TGCATGAAGC CTGTCTGCTT CAGCGCTGCC ACAAGCCCAA GAAGCTGCAG TGGCTGGTGT 
     4201 CCGCCTGGTC CCAGTGCTCT GTGACATGTG AAAGAGGAAC ACAGAAAAGA TTCTTAAAAT 
     4261 GTGCTGAAAA GTATGTTTCT GGAAAGTATC GAGAGCTGGC CTCAAAGAAG TGCTCACATT 
     4321 TGCCGAAGCC CAGCCTGGAG CTGGAACGTG CCTGCGCCCC GCTTCCATGC CCCAGGCACC 
     4381 CCCCATTTGC TGCTGCGGGA CCCTCGAGGG GCAGCTGGTT TGCCTCACCC TGGTCTCAGT 
     4441 GCACGGCCAG CTGTGGGGGA GGCGTTCAGA CGAGGTCCGT GCAGTGCCTG GCTGGGGGCC 
     4501 GGCCGGCCTC AGGCTGCCTC CTGCACCAGA AGCCTTCGGC CTCCCTGGCC TGCAACACTC 
     4561 ACTTCTGCCC CATTGCAGAG AAGAAAGATG CCTTCTGCAA AGACTACTTC CACTGGTGCT 
     4621 ACCTGGTACC CCAGCACGGG ATGTGCAGCC ACAAGTTCTA CGGCAAGCAG TGCTGCAAGA 
     4681 CTTGCTCTAA GTCCAACTTG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4741 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4801 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4861 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4921 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4981 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5041 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5101 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5161 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5221 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5281 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5341 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5401 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5461 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5521 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5581 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5641 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5701 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5761 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5821 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5881 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5941 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6001 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6061 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6121 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6181 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6241 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6301 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6361 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6421 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6481 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6541 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6601 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6661 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6721 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6781 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6841 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6901 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6961 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7021 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7081 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7141 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7201 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7261 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7321 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7381 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7441 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7501 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7561 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7621 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7681 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7741 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7801 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7861 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7921 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7981 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8041 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8101 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8161 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8221 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8281 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8341 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8401 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8461 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8521 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//