LOCUS       RC221591                9240 bp    DNA     circular  SYN 
DEFINITION  MLH3 (Myc-DDK-tagged)-Human mutL homolog 3 (E. coli) (MLH3), transcript variant 1
ACCESSION   NM_001040108
VERSION     NM_001040108.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5397..5488 
                     /label="Myc-DDK"  
     misc_feature    5518..6103 
                     /label="PolyA signal"  
     misc_feature    5575..5594 
                     /label="Restriction Enzyme"  
     misc_feature    6379..7051 
                     /label="ColE1"  
     misc_feature    7481..8275 
                     /label="Kan/Neo"  
     misc_feature    8310..8612 
                     /label="SV40 ori"  
     misc_feature    8698..9175 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5379..5385 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5379 
                     /label="MLH3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATCAAGTGC TTGTCAGTTG AAGTACAAGC CAAATTGCGT TCTGGTTTGG 
     1081 CCATAAGCTC CTTGGGCCAA TGTGTTGAGG AACTTGCCCT CAACAGTATT GATGCTGAAG 
     1141 CAAAATGTGT GGCTGTCAGG GTGAATATGG AAACCTTCCA AGTTCAAGTG ATAGACAATG 
     1201 GATTTGGGAT GGGGAGTGAT GATGTAGAGA AAGTGGGAAA TCGTTATTTC ACCAGTAAAT 
     1261 GCCACTCGGT ACAGGACTTG GAGAATCCAA GGTTTTATGG TTTCCGAGGA GAGGCCTTGG 
     1321 CAAATATTGC TGACATGGCC AGTGCTGTGG AAATTTCGTC CAAGAAAAAC AGGACAATGA 
     1381 AAACTTTTGT GAAACTGTTT CAGAGTGGAA AAGCCCTGAA AGCTTGTGAA GCTGATGTGA 
     1441 CTAGAGCAAG CGCTGGGACT ACTGTAACAG TGTATAACCT ATTTTACCAG CTTCCTGTAA 
     1501 GGAGGAAATG CATGGACCCT AGACTGGAGT TTGAGAAGGT TAGGCAGAGA ATAGAAGCTC 
     1561 TCTCACTCAT GCACCCTTCC ATTTCTTTCT CTTTGAGAAA TGATGTTTCT GGTTCCATGG 
     1621 TTCTTCAGCT CCCTAAAACC AAAGACGTAT GTTCCCGATT TTGTCAAATT TATGGATTGG 
     1681 GAAAGTCCCA AAAGCTAAGA GAAATAAGTT TTAAATATAA AGAGTTTGAG CTTAGTGGCT 
     1741 ATATCAGCTC TGAAGCACAT TACAACAAGA ATATGCAGTT TTTGTTTGTG AACAAAAGAC 
     1801 TAGTTTTAAG GACAAAGCTA CATAAACTCA TTGACTTTTT ATTAAGGAAA GAAAGTATTA 
     1861 TATGCAAGCC AAAGAATGGT CCCACCAGTA GGCAAATGAA TTCAAGTCTT CGGCACCGGT 
     1921 CTACCCCAGA ACTCTATGGC ATATATGTAA TTAATGTGCA GTGCCAATTC TGTGAGTATG 
     1981 ATGTGTGCAT GGAGCCAGCC AAAACTCTGA TTGAATTTCA GAACTGGGAC ACTCTCTTGT 
     2041 TTTGCATTCA GGAAGGAGTG AAAATGTTTT TAAAGCAAGA AAAATTATTT GTGGAATTAT 
     2101 CAGGTGAGGA TATTAAGGAA TTTAGTGAAG ATAATGGTTT TAGTTTATTT GATGCTACTC 
     2161 TTCAGAAGCG TGTGACTTCC GATGAGAGGA GCAATTTCCA GGAAGCATGT AATAATATTT 
     2221 TAGATTCCTA TGAGATGTTT AATTTGCAGT CAAAAGCTGT GAAAAGAAAA ACTACTGCAG 
     2281 AAAACGTAAA CACACAGAGT TCTAGGGATT CAGAAGCTAC CAGAAAAAAT ACAAATGATG 
     2341 CATTTTTGTA CATTTATGAA TCAGGTGGTC CAGGCCATAG CAAAATGACA GAGCCATCTT 
     2401 TACAAAACAA AGACAGCTCT TGCTCAGAAT CAAAGATGTT AGAACAAGAG ACAATTGTAG 
     2461 CATCAGAAGC TGGAGAAAAT GAGAAACATA AAAAATCTTT CCTGGAACAT AGCTCTTTAG 
     2521 AAAATCCGTG TGGAACCAGT TTAGAAATGT TTTTAAGCCC TTTTCAGACA CCATGTCACT 
     2581 TTGAGGAGAG TGGGCAGGAT CTAGAAATAT GGAAAGAAAG TACTACTGTT AATGGCATGG 
     2641 CTGCCAACAT CTTGAAAAAT AATAGAATTC AGAATCAACC AAAGAGATTT AAAGATGCTA 
     2701 CTGAAGTGGG ATGCCAGCCT CTGCCTTTTG CAACAACATT ATGGGGAGTA CATAGTGCTC 
     2761 AGACAGAGAA AGAGAAAAAA AAAGAATCTA GCAATTGTGG AAGAAGAAAT GTTTTTAGTT 
     2821 ATGGGCGAGT TAAATTATGT TCCACTGGCT TTATAACTCA TGTAGTACAA AATGAAAAAA 
     2881 CTAAATCAAC TGAAACAGAA CATTCATTTA AAAATTATGT TAGACCTGGT CCCACACGTG 
     2941 CCCAAGAAAC ATTTGGAAAT AGAACACGTC ATTCAGTTGA AACTCCAGAC ATCAAAGATT 
     3001 TAGCCAGCAC TTTAAGTAAA GAATCTGGTC AATTGCCCAA CAAAAAAAAT TGCAGAACGA 
     3061 ATATAAGTTA TGGGCTAGAG AATGAACCTA CAGCAACTTA TACAATGTTT TCTGCTTTTC 
     3121 AGGAAGGTAG CAAAAAATCA CAAACAGATT GCATATTATC TGATACATCC CCCTCTTTCC 
     3181 CCTGGTATAG ACACGTTTCC AATGATAGTA GGAAAACAGA TAAATTAATT GGTTTCTCCA 
     3241 AACCAATCGT CCGTAAGAAG CTAAGCTTGA GTTCACAGCT AGGATCTTTA GAGAAGTTTA 
     3301 AGAGGCAATA TGGGAAGGTT GAAAATCCTC TGGATACAGA AGTAGAGGAA AGTAATGGAG 
     3361 TCACTACCAA TCTCAGTCTT CAAGTTGAAC CTGACATTCT GCTGAAGGAC AAGAACCGCT 
     3421 TAGAGAACTC TGATGTTTGT AAAATCACTA CTATGGAGCA TAGTGATTCA GATAGTAGTT 
     3481 GTCAACCAGC AAGCCACATC CTTAACTCAG AGAAGTTTCC ATTCTCCAAG GATGAAGATT 
     3541 GTTTAGAACA ACAGATGCCT AGTTTGAGAG AAAGTCCTAT GACCCTGAAG GAGTTATCTC 
     3601 TCTTTAATAG AAAACCTTTG GACCTTGAGA AGTCATCTGA ATCACTAGCC TCTAAATTAT 
     3661 CCAGACTGAA GGGTTCCGAA AGAGAAACTC AAACAATGGG GATGATGAGT CGTTTTAATG 
     3721 AACTTCCAAA TTCAGATTCC AGTAGGAAAG ACAGCAAGTT GTGCAGTGTG TTAACACAAG 
     3781 ATTTTTGTAT GTTATTTAAC AACAAGCATG AAAAAACAGA GAATGGTGTC ATCCCAACAT 
     3841 CAGATTCTGC CACACAGGAT AATTCCTTTA ATAAAAATAG TAAAACACAT TCTAACAGCA 
     3901 ATACAACAGA GAACTGTGTG ATATCAGAAA CTCCTTTGGT ATTGCCCTAT AATAATTCTA 
     3961 AAGTTACCGG TAAAGATTCA GATGTTCTTA TCAGAGCCTC AGAACAACAG ATAGGAAGTC 
     4021 TTGACTCTCC CAGTGGAATG TTAATGAATC CGGTAGAAGA TGCCACAGGT GACCAAAATG 
     4081 GAATTTGTTT TCAGAGTGAG GAATCTAAAG CAAGAGCTTG TTCTGAAACT GAAGAGTCAA 
     4141 ACACGTGTTG TTCAGATTGG CAGCGGCATT TCGATGTAGC CCTGGGAAGA ATGGTTTATG 
     4201 TCAACAAAAT GACTGGACTC AGCACATTCA TTGCCCCAAC TGAGGACATT CAGGCTGCTT 
     4261 GTACTAAAGA CCTGACAACT GTGGCTGTGG ATGTTGTACT TGAGAATGGG TCTCAGTACA 
     4321 GGTGTCAACC TTTTAGAAGC GACCTTGTTC TTCCTTTCCT TCCGAGAGCT CGAGCAGAGA 
     4381 GGACTGTGAT GAGACAGGAT AACAGAGATA CTGTGGATGA TACTGTTAGT AGCGAATCGC 
     4441 TTCAGTCTTT GTTCTCAGAA TGGGACAATC CAGTATTTGC CCGTTATCCA GAGGTTGCTG 
     4501 TTGATGTAAG CAGTGGCCAG GCTGAGAGCT TAGCAGTTAA AATTCACAAC ATCTTGTATC 
     4561 CCTATCGTTT CACCAAAGGA ATGATTCATT CAATGCAGGT TCTCCAGCAA GTAGATAACA 
     4621 AGTTTATTGC CTGTTTGATG AGCACTAAGA CTGAAGAGAA TGGCGAGGCA GGTGGGAACC 
     4681 TGCTCGTGCT GGTGGATCAG CACGCTGCCC ATGAGCGTAT ACGTCTGGAG CAGCTTATCA 
     4741 TTGATTCCTA CGAGAAGCAA CAGGCACAAG GCTCTGGTCG GAAAAAATTA CTGTCTTCTA 
     4801 CTCTAATTCC TCCGCTAGAG ATAACAGTGA CAGAGGAACA AAGGAGACTC TTATGGTGTT 
     4861 ACCACAAAAA TCTGGAAGAT CTGGGCCTTG AATTTGTATT TCCAGACACT AGTGATTCTC 
     4921 TGGTCCTTGT GGGAAAAGTA CCACTATGTT TTGTGGAAAG AGAAGCCAAT GAACTTCGGA 
     4981 GAGGAAGATC TACTGTGACC AAGAGTATTG TGGAGGAATT TATCCGAGAA CAACTGGAGC 
     5041 TACTCCAGAC CACCGGAGGC ATCCAAGGGA CATTGCCACT GACTGTCCAG AAGGTGTTGG 
     5101 CATCCCAAGC CTGCCATGGG GCCATTAAGT TTAATGATGG CCTGAGCTTA CAGGAAAGTT 
     5161 GCCGCCTTAT TGAAGCTCTG TCCTCATGCC AGCTGCCATT CCAGTGTGCT CACGGGAGAC 
     5221 CTTCTATGCT GCCGTTAGCT GACATAGACC ACTTGGAACA GGAAAAACAG ATTAAACCCA 
     5281 ACCTCACTAA ACTTCGCAAA ATGGCCCAGG CCTGGCGTCT CTTTGGAAAA GCAGAGTGTG 
     5341 ATACAAGGCA GAGCCTGCAG CAATCCATGC CTCCCTGTGA GCCACCAACG CGTACGCGGC 
     5401 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5461 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5521 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5581 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5641 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5701 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5761 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5821 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5881 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5941 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     6001 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     6061 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     6121 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     6181 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     6241 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     6301 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     6361 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6421 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6481 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6541 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6601 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6661 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6721 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6781 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6841 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6901 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6961 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     7021 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     7081 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     7141 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     7201 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     7261 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     7321 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     7381 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7441 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7501 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7561 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7621 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7681 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7741 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7801 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7861 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7921 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7981 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     8041 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     8101 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     8161 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     8221 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     8281 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     8341 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     8401 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8461 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8521 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8581 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8641 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8701 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8761 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8821 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8881 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8941 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     9001 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     9061 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     9121 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     9181 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//