LOCUS       RC221568                9474 bp    DNA     circular  SYN 
DEFINITION  TOP2A (Myc-DDK-tagged)-Human topoisomerase (DNA) II alpha 170kDa (TOP2A)
ACCESSION   NM_001067
VERSION     NM_001067.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5631..5722 
                     /label="Myc-DDK"  
     misc_feature    5752..6337 
                     /label="PolyA signal"  
     misc_feature    5809..5828 
                     /label="Restriction Enzyme"  
     misc_feature    6613..7285 
                     /label="ColE1"  
     misc_feature    7715..8509 
                     /label="Kan/Neo"  
     misc_feature    8544..8846 
                     /label="SV40 ori"  
     misc_feature    8932..9409 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5613..5619 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5613 
                     /label="TOP2A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGTGTCA CCATTGCAGC CTGTAAATGA AAATATGCAA GTCAACAAAA 
     1081 TAAAGAAAAA TGAAGATGCT AAGAAAAGAC TGTCTGTTGA AAGAATCTAT CAAAAGAAAA 
     1141 CACAATTGGA ACATATTTTG CTCCGCCCAG ACACCTACAT TGGTTCTGTG GAATTAGTGA 
     1201 CCCAGCAAAT GTGGGTTTAC GATGAAGATG TTGGCATTAA CTATAGGGAA GTCACTTTTG 
     1261 TTCCTGGTTT GTACAAAATC TTTGATGAGA TTCTAGTTAA TGCTGCGGAC AACAAACAAA 
     1321 GGGACCCAAA AATGTCTTGT ATTAGAGTCA CAATTGATCC GGAAAACAAT TTAATTAGTA 
     1381 TATGGAATAA TGGAAAAGGT ATTCCTGTTG TTGAACACAA AGTTGAAAAG ATGTATGTCC 
     1441 CAGCTCTCAT ATTTGGACAG CTCCTAACTT CTAGTAACTA TGATGATGAT GAAAAGAAAG 
     1501 TGACAGGTGG TCGAAATGGC TATGGAGCCA AATTGTGTAA CATATTCAGT ACCAAATTTA 
     1561 CTGTGGAAAC AGCCAGTAGA GAATACAAGA AAATGTTCAA ACAGACATGG ATGGATAATA 
     1621 TGGGAAGAGC TGGTGAGATG GAACTCAAGC CCTTCAATGG AGAAGATTAT ACATGTATCA 
     1681 CCTTTCAGCC TGATTTGTCT AAGTTTAAAA TGCAAAGCCT GGACAAAGAT ATTGTTGCAC 
     1741 TAATGGTCAG AAGAGCATAT GATATTGCTG GATCCACCAA AGATGTCAAA GTCTTTCTTA 
     1801 ATGGAAATAA ACTGCCAGTA AAAGGATTTC GTAGTTATGT GGACATGTAT TTGAAGGACA 
     1861 AGTTGGATGA AACTGGTAAC TCCTTGAAAG TAATACATGA ACAAGTAAAC CACAGGTGGG 
     1921 AAGTGTGTTT AACTATGAGT GAAAAAGGCT TTCAGCAAAT TAGCTTTGTC AACAGCATTG 
     1981 CTACATCCAA GGGTGGCAGA CATGTTGATT ATGTAGCTGA TCAGATTGTG ACTAAACTTG 
     2041 TTGATGTTGT GAAGAAGAAG AACAAGGGTG GTGTTGCAGT AAAAGCACAT CAGGTGAAAA 
     2101 ATCACATGTG GATTTTTGTA AATGCCTTAA TTGAAAACCC AACCTTTGAC TCTCAGACAA 
     2161 AAGAAAACAT GACTTTACAA CCCAAGAGCT TTGGATCAAC ATGCCAATTG AGTGAAAAAT 
     2221 TTATCAAAGC TGCCATTGGC TGTGGTATTG TAGAAAGCAT ACTAAACTGG GTGAAGTTTA 
     2281 AGGCCCAAGT CCAGTTAAAC AAGAAGTGTT CAGCTGTAAA ACATAATAGA ATCAAGGGAA 
     2341 TTCCCAAACT CGATGATGCC AATGATGCAG GGGGCCGAAA CTCCACTGAG TGTACGCTTA 
     2401 TCCTGACTGA GGGAGATTCA GCCAAAACTT TGGCTGTTTC AGGCCTTGGT GTGGTTGGGA 
     2461 GAGACAAATA TGGGGTTTTC CCTCTTAGAG GAAAAATACT CAATGTTCGA GAAGCTTCTC 
     2521 ATAAGCAGAT CATGGAAAAT GCTGAGATTA ACAATATCAT CAAGATTGTG GGTCTTCAGT 
     2581 ACAAGAAAAA CTATGAAGAT GAAGATTCAT TGAAGACGCT TCGTTATGGG AAGATAATGA 
     2641 TTATGACAGA TCAGGACCAA GATGGTTCCC ACATCAAAGG CTTGCTGATT AATTTTATCC 
     2701 ATCACAACTG GCCCTCTCTT CTGCGACATC GTTTTCTGGA GGAATTTATC ACTCCCATTG 
     2761 TAAAGGTATC TAAAAACAAG CAAGAAATGG CATTTTACAG CCTTCCTGAA TTTGAAGAGT 
     2821 GGAAGAGTTC TACTCCAAAT CATAAAAAAT GGAAAGTCAA ATATTACAAA GGTTTGGGCA 
     2881 CCAGCACATC AAAGGAAGCT AAAGAATACT TTGCAGATAT GAAAAGACAT CGTATCCAGT 
     2941 TCAAATATTC TGGTCCTGAA GATGATGCTG CTATCAGCCT GGCCTTTAGC AAAAAACAGA 
     3001 TAGATGATCG AAAGGAATGG TTAACTAATT TCATGGAGGA TAGAAGACAA CGAAAGTTAC 
     3061 TTGGGCTTCC TGAGGATTAC TTGTATGGAC AAACTACCAC ATATCTGACA TATAATGACT 
     3121 TCATCAACAA GGAACTTATC TTGTTCTCAA ATTCTGATAA CGAGAGATCT ATCCCTTCTA 
     3181 TGGTGGATGG TTTGAAACCA GGTCAGAGAA AGGTTTTGTT TACTTGCTTC AAACGGAATG 
     3241 ACAAGCGAGA AGTAAAGGTT GCCCAATTAG CTGGATCAGT GGCTGAAATG TCTTCTTATC 
     3301 ATCATGGTGA GATGTCACTA ATGATGACCA TTATCAATTT GGCTCAGAAT TTTGTGGGTA 
     3361 GCAATAATCT AAACCTCTTG CAGCCCATTG GTCAGTTTGG TACCAGGCTA CATGGTGGCA 
     3421 AGGATTCTGC TAGTCCACGA TACATCTTTA CAATGCTCAG CTCTTTGGCT CGATTGTTAT 
     3481 TTCCACCAAA AGATGATCAC ACGTTGAAGT TTTTATATGA TGACAACCAG CGTGTTGAGC 
     3541 CTGAATGGTA CATTCCTATT ATTCCCATGG TGCTGATAAA TGGTGCTGAA GGAATCGGTA 
     3601 CTGGGTGGTC CTGCAAAATC CCCAACTTTG ATGTGCGTGA AATTGTAAAT AACATCAGGC 
     3661 GTTTGATGGA TGGAGAAGAA CCTTTGCCAA TGCTTCCAAG TTACAAGAAC TTCAAGGGTA 
     3721 CTATTGAAGA ACTGGCTCCA AATCAATATG TGATTAGTGG TGAAGTAGCT ATTCTTAATT 
     3781 CTACAACCAT TGAAATCTCA GAGCTTCCCG TCAGAACATG GACCCAGACA TACAAAGAAC 
     3841 AAGTTCTAGA ACCCATGTTG AATGGCACCG AGAAGACACC TCCTCTCATA ACAGACTATA 
     3901 GGGAATACCA TACAGATACC ACTGTGAAAT TTGTTGTGAA GATGACTGAA GAAAAACTGG 
     3961 CAGAGGCAGA GAGAGTTGGA CTACACAAAG TCTTCAAACT CCAAACTAGT CTCACATGCA 
     4021 ACTCTATGGT GCTTTTTGAC CACGTAGGCT GTTTAAAGAA ATATGACACG GTGTTGGATA 
     4081 TTCTAAGAGA CTTTTTTGAA CTCAGACTTA AATATTATGG ATTAAGAAAA GAATGGCTCC 
     4141 TAGGAATGCT TGGTGCTGAA TCTGCTAAAC TGAATAATCA GGCTCGCTTT ATCTTAGAGA 
     4201 AAATAGATGG CAAAATAATC ATTGAAAATA AGCCTAAGAA AGAATTAATT AAAGTTCTGA 
     4261 TTCAGAGGGG ATATGATTCG GATCCTGTGA AGGCCTGGAA AGAAGCCCAG CAAAAGGTTC 
     4321 CAGATGAAGA AGAAAATGAA GAGAGTGACA ACGAAAAGGA AACTGAAAAG AGTGACTCCG 
     4381 TAACAGATTC TGGACCAACC TTCAACTATC TTCTTGATAT GCCCCTTTGG TATTTAACCA 
     4441 AGGAAAAGAA AGATGAACTC TGCAGGCTAA GAAATGAAAA AGAACAAGAG CTGGACACAT 
     4501 TAAAAAGAAA GAGTCCATCA GATTTGTGGA AAGAAGACTT GGCTACATTT ATTGAAGAAT 
     4561 TGGAGGCTGT TGAAGCCAAG GAAAAACAAG ATGAACAAGT CGGACTTCCT GGGAAAGGGG 
     4621 GGAAGGCCAA GGGGAAAAAA ACACAAATGG CTGAAGTTTT GCCTTCTCCG CGTGGTCAAA 
     4681 GAGTCATTCC ACGAATAACC ATAGAAATGA AAGCAGAGGC AGAAAAGAAA AATAAAAAGA 
     4741 AAATTAAGAA TGAAAATACT GAAGGAAGCC CTCAAGAAGA TGGTGTGGAA CTAGAAGGCC 
     4801 TAAAACAAAG ATTAGAAAAG AAACAGAAAA GAGAACCAGG TACAAAGACA AAGAAACAAA 
     4861 CTACATTGGC ATTTAAGCCA ATCAAAAAAG GAAAGAAGAG AAATCCCTGG TCTGATTCAG 
     4921 AATCAGATAG GAGCAGTGAC GAAAGTAATT TTGATGTCCC TCCACGAGAA ACAGAGCCAC 
     4981 GGAGAGCAGC AACAAAAACA AAATTCACAA TGGATTTGGA TTCAGATGAA GATTTCTCAG 
     5041 ATTTTGATGA AAAAACTGAT GATGAAGATT TTGTCCCATC AGATGCTAGT CCACCTAAGA 
     5101 CCAAAACTTC CCCAAAACTT AGTAACAAAG AACTGAAACC ACAGAAAAGT GTCGTGTCAG 
     5161 ACCTTGAAGC TGATGATGTT AAGGGCAGTG TACCACTGTC TTCAAGCCCT CCTGCTACAC 
     5221 ATTTCCCAGA TGAAACTGAA ATTACAAACC CAGTTCCTAA AAAGAATGTG ACAGTGAAGA 
     5281 AGACAGCAGC AAAAAGTCAG TCTTCCACCT CCACTACCGG TGCCAAAAAA AGGGCTGCCC 
     5341 CAAAAGGAAC TAAAAGGGAT CCAGCTTTGA ATTCTGGTGT CTCTCAAAAG CCTGATCCTG 
     5401 CCAAAACCAA GAATCGCCGC AAAAGGAAGC CATCCACTTC TGATGATTCT GACTCTAATT 
     5461 TTGAGAAAAT TGTTTCGAAA GCAGTCACAA GCAAGAAATC CAAGGGGGAG AGTGATGACT 
     5521 TCCATATGGA CTTTGACTCA GCTGTGGCTC CTCGGGCAAA ATCTGTACGG GCAAAGAAAC 
     5581 CTATAAAGTA CCTGGAAGAG TCAGATGAAG ATGATCTGTT TACGCGTACG CGGCCGCTCG 
     5641 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5701 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5761 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5821 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5881 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5941 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     6001 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     6061 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     6121 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     6181 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     6241 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     6301 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     6361 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     6421 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     6481 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     6541 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6601 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6661 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6721 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6781 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6841 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6901 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6961 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     7021 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     7081 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     7141 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     7201 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     7261 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     7321 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     7381 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     7441 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     7501 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     7561 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7621 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7681 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7741 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7801 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7861 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7921 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7981 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     8041 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     8101 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     8161 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     8221 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     8281 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     8341 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     8401 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     8461 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     8521 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     8581 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8641 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8701 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8761 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8821 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8881 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8941 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     9001 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     9061 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     9121 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     9181 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     9241 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     9301 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     9361 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     9421 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//