LOCUS       RC221527                7950 bp    DNA     circular  SYN 
DEFINITION  TMEM132A (Myc-DDK-tagged)-Human transmembrane protein 132A (TMEM132A), transcript variant 2
ACCESSION   NM_178031
VERSION     NM_178031.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4107..4198 
                     /label="Myc-DDK"  
     misc_feature    4228..4813 
                     /label="PolyA signal"  
     misc_feature    4285..4304 
                     /label="Restriction Enzyme"  
     misc_feature    5089..5761 
                     /label="ColE1"  
     misc_feature    6191..6985 
                     /label="Kan/Neo"  
     misc_feature    7020..7322 
                     /label="SV40 ori"  
     misc_feature    7408..7885 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4089..4095 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4089 
                     /label="TMEM132A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGCGCGCGG ATGGCCGGTC GCACAACAGC GGCCCCTCGG GGGCCCTACG 
     1081 GCCCCTGGCT CTGCCTCCTG GTGGCCCTCG CCCTGGACGT CGTGAGAGTG GACTGTGGCC 
     1141 AGGCTCCCCT GGACCCTGTC TACCTGCCGG CAGCCCTGGA GCTCCTAGAC GCCCCTGAAC 
     1201 ACTTCCGTGT GCAGCAGGTG GGCCACTACC CACCTGCCAA CTCCTCTCTG AGCTCCCGAT 
     1261 CTGAGACCTT TCTGCTCCTA CAGCCCTGGC CCAGGGCCCA GCCACTTCTC CGGGCCTCCT 
     1321 ACCCACCTTT TGCCACTCAG CAGGTGGTCC CCCCTCGAGT CACTGAGCCC CACCAACGGC 
     1381 CAGTCCCATG GGACGTGCGG GCCGTTTCAG TGGAAGCGGC TGTGACTCCA GCAGAGCCCT 
     1441 ACGCCCGGGT TCTCTTCCAC CTCAAAGGGC AGGATTGGCC ACCAGGGTCT GGCAGCCTGC 
     1501 CCTGTGCCCG GCTCCATGCC ACACACCCTG CAGGCACTGC TCACCAAGCC TGCCGCTTCC 
     1561 AGCCATCCCT GGGCGCCTGC GTGGTGGAGC TGGAGCTTCC CTCGCACTGG TTCTCACAGG 
     1621 CCTCCACCAC ACGGGCCGAG CTGGCCTACA CGCTTGAGCC TGCAGCTGAG GGCCCTGGGG 
     1681 GCTGTGGCTC CGGCGAGGAG AACGACCCTG GGGAGCAGGC CCTCCCAGTG GGGGGTGTGG 
     1741 AGCTGCGCCC AGCAGACCCC CCGCAGTACC AGGAGGTACC TCTGGACGAG GCTGTGACTC 
     1801 TGCGGGTGCC TGACATGCCA GTGCGGCCCG GCCAGCTCTT TAGTGCTACC CTCCTGCTTC 
     1861 GGCACAACTT CACAGCCAGC CTCCTGACCC TGCGGATCAA GGTGAAGAAG GGGCTGCATG 
     1921 TGACAGCCGC CCGCCCAGCC CAGCCCACAC TCTGGACTGC CAAGCTAGAC CGCTTCAAGG 
     1981 GCTCCAGGCA CCACACCACC CTCATCACCT GCCACCGTGC TGGGCTCACA GAGCCAGATT 
     2041 CCAGTCCCCT TGAACTGTCT GAGTTCCTAT GGGTGGACTT TGTGGTGGAG AATAGCACTG 
     2101 GTGGGGGCGT AGCGGTCACT CGCCCCGTCA CGTGGCAGCT GGAGTACCCA GGCCAGGCCC 
     2161 CTGAAGCAGA GAAGGACAAA ATGGTGTGGG AAATCCTGGT GTCTGAGCGG GACATCAGAG 
     2221 CCCTTATCCC ACTGGCCAAG GCTGAGGAGC TGGTGAATAC AGCACCACTG ACTGGAGTGC 
     2281 CCCAGCATGT CCCCGTGCGC CTTGTCACTG TGGACGGCGG GGGGGCCTTG GTGGAGGTGA 
     2341 CAGAGCATGT CGGCTGCGAG TCTGCCAACA CACAGGTCCT GCAGGTGTCT GAGGCCTGTG 
     2401 ATGCCGTGTT CGTGGCTGGC AAGGAGAGCC GGGGCGCCCG GGGGGTGCGA GTGGACTTCT 
     2461 GGTGGCGCCG GCTCCGCGCC TCGCTGCGGC TGACCGTGTG GGCCCCCCTG CTACCGCTGC 
     2521 GTATCGAGCT CACCGACACC ACCCTCGAGC AGGTCCGCGG CTGGAGGGTA CCTGGCCCTG 
     2581 CTGAAGGGCC TGCGGAACCC GCTGCAGAGG CGTCGGATGA GGCCGAGCGG CGCGCCCGTG 
     2641 GCTGCCACCT GCAGTACCAG CGGGCCGGTG TGCGCTTCCT CGCCCCCTTC GCGGCCCACC 
     2701 CGCTGGACGG CGGCCGCCGC CTCACGCACC TGCTTGGCCC CGACTGGCTG CTAGACGTGT 
     2761 CCCACCTCGT GGCGCCACAC GCCCGCGTGC TGGACTCGCG TGTAGCCTCT CTGGAGGGTG 
     2821 GCCGTGTCGT GGTGGGCCGG GAGCCCGGTG TCACCTCCAT TGAGGTGCGT TCCCCACTGT 
     2881 CTGACTCCAT CCTGGGGGAG CAGGCGCTGG CTGTGACGGA CGACAAGGTC TCAGTGCTGG 
     2941 AGCTGAGGGT GCAGCCAGTG ATGGGCATCT CGCTGACCTT GAGCCGGGGC ACTGCCCACC 
     3001 CCGGGGAGGT CACAGCTACG TGCTGGGCAC AGTCAGCCCT TCCCGCCCCA AAGCAGGAGG 
     3061 TGGCCCTCTC CCTATGGCTG TCCTTCTCTG ATCACACTGT GGCCCCAGCT GAGCTCTACG 
     3121 ACCGCCGTGA CCTGGGACTG TCCGTCTCAG CCGAGGAGCC TGGTGCCATC CTGCCAGCTG 
     3181 AGGAGCAGGG TGCCCAGCTC GGGGTGGTGG TGAGTGGGGC AGGCGCCGAG GGGCTGCCGC 
     3241 TGCATGTGGC TCTGCACCCG CCCGAGCCCT GCCGCCGGGG CCGCCACCGT GTGCCTCTGG 
     3301 CCTCTGGCAC CGCCTGGCTG GGGCTGCCCC CTGCCTCCAC TCCAGCCCCT GCTCTCCCAT 
     3361 CCAGCCCTGC TTGGAGCCCA CCAGCCACAG AAGCCACCAT GGGTGGTAAA CGGCAGGTGG 
     3421 CAGGCAGTGT CGGGGGCAAC ACAGGTGTGA GGGGCAAGTT TGAGCGGGCA GAGGAGGAGG 
     3481 CCAGGAAGGA GGAGACCGAA GCCAGGGAGG AGGAGGAGGA AGAGGAGGAG GAGATGGTCC 
     3541 CTGCCCCTCA GCATGTCACT GAGCTAGAGC TGGGCATGTA CGCCCTGCTG GGAGTCTTCT 
     3601 GCGTGGCCAT CTTCATCTTC TTGGTCAATG GTGTGGTCTT CGTCCTGCGC TATCAGCGCA 
     3661 AAGAACCTCC CGACAGTGCC ACTGACCCCA CCTCCCCCCA GCCCCACAAC TGGGTCTGGC 
     3721 TGGGCACTGA CCAGGAGGAA CTGAGCCGCC AGCTGGACCG GCAGTCCCCT GGCCCGCCCA 
     3781 AGGGGGAGGG GAGCTGCCCC TGTGAGAGTG GGGGAGGAGG GGAGGCCCCT ACCCTGGCCC 
     3841 CTGGCCCTCC TGGGGGCACC ACCAGCTCCT CAAGCACCCT GGCCCGAAAG GAGGCTGGGG 
     3901 GGCGGCGGAA GCGAGTAGAG TTTGTGACAT TTGCGCCAGC CCCTCCAGCC CAGTCACCTG 
     3961 AGGAGCCTGT AGGGGCCCCT GCTGTGCAGT CCATCCTTGT GGCAGGCGAG GAGGACATCC 
     4021 GCTGGGTGTG TGAGGACATG GGGCTGAAGG ACCCTGAGGA GCTTCGCAAC TACATGGAGA 
     4081 GGATCCGGGG CAGCTCCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4141 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4201 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4261 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4321 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4381 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4441 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4501 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4561 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4621 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4681 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4741 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4801 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4861 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4921 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4981 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5041 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5101 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5161 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5221 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5281 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5341 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5401 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5461 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5521 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5581 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5641 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5701 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5761 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5821 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5881 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5941 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6001 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6061 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6121 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6181 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6241 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6301 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6361 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6421 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6481 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6541 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6601 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6661 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6721 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6781 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6841 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6901 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6961 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7021 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7081 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7141 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7201 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7261 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7321 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7381 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7441 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7501 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7561 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7621 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7681 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7741 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7801 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7861 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7921 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//