LOCUS       RC221432                8460 bp    DNA     circular  SYN 
DEFINITION  DIAPH3 (Myc-DDK-tagged)-Human diaphanous homolog 3 (Drosophila) (DIAPH3), transcript variant 1
ACCESSION   NM_001042517
VERSION     NM_001042517.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4617..4708 
                     /label="Myc-DDK"  
     misc_feature    4738..5323 
                     /label="PolyA signal"  
     misc_feature    4795..4814 
                     /label="Restriction Enzyme"  
     misc_feature    5599..6271 
                     /label="ColE1"  
     misc_feature    6701..7495 
                     /label="Kan/Neo"  
     misc_feature    7530..7832 
                     /label="SV40 ori"  
     misc_feature    7918..8395 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4599..4605 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4599 
                     /label="DIAPH3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACGGCAC CAGCCGCGGC TGCACCACCC GGCCCAAGGC TCAGCCGCTG 
     1081 GGACTCCCTA CCCTTCCTCA GCCTCTCTCC GCGGCTGCCG GGAAAGCAAG ATGCCGCGCA 
     1141 GGAAGGGCCC CCAACACCCT CCGCCGCCCA GTGGCCCCGA GGAGCCTGGG GAGAAGCGCC 
     1201 CCAAGTTTCA TTTAAATATT AGGACACTGA CGGATGATAT GCTGGACAAA TTTGCCAGCA 
     1261 TAAGAATTCC AGGGAGCAAG AAAGAGAGAC CTCCACTTCC CAACCTGAAG ACTGCATTTG 
     1321 CAAGCAGTGA TTGCTCAGCA GCACCTTTAG AGATGATGGA GAACTTTCCA AAGCCACTGT 
     1381 CAGAGAATGA ACTCTTAGAA CTTTTTGAAA AAATGATGGA AGATATGAAT TTAAATGAAG 
     1441 ATAAAAAGGC ACCATTGCGG GAAAAGGACT TCAGTATCAA AAAAGAAATG GTGATGCAGT 
     1501 ACATTAATAC TGCTTCTAAG ACAGGAAGTC TTAAGAGAAG CCGACAGATC TCACCTCAGG 
     1561 AATTCATTCA TGAGCTGAAA ATGGGGTCTG CAGATGAGAG ACTTGTCACA TGCCTGGAGT 
     1621 CTCTCCGAGT GTCTTTGACC AGCAATCCTG TGAGTTGGGT GGAAAGCTTT GGACATGAAG 
     1681 GGCTTGGATT ATTATTAGAC ATTTTGGAAA AACTGATTAG TGGAAAAATC CAAGAAAAAG 
     1741 TTGTAAAGAA AAATCAACAT AAAGTCATAC AGTGTCTAAA AGCCCTGATG AATACGCAGT 
     1801 ATGGCTTGGA AAGAATTATG AGTGAGGAGA GGAGCCTTTC CTTATTGGCC AAAGCCGTGG 
     1861 ATCCCAGACA CCCCAATATG ATGACAGATG TGGTTAAACT TCTCTCTGCG GTATGCATTG 
     1921 TAGGGGAAGA AAGCATCCTT GAAGAAGTTT TAGAAGCTTT AACTTCAGCT GGTGAAGAAA 
     1981 AAAAAATTGA CAGATTTTTT TGTATTGTGG AAGGCCTCCG GCACAATTCA GTTCAACTGC 
     2041 AAGTAGCTTG TATGCAGCTC ATCAATGCCC TGGTTACATC TCCTGATGAT TTGGATTTCA 
     2101 GGCTTCACAT CAGAAATGAA TTTATGCGTT GTGGATTGAA AGAGATATTG CCAAATTTAA 
     2161 AATGCATTAA GAATGATGGC CTGGATATCC AACTTAAAGT CTTTGATGAG CATAAAGAAG 
     2221 AAGATTTGTT TGAGTTATCC CATCGCCTTG AAGATATTAG AGCTGAACTT GATGAAGCAT 
     2281 ATGATGTTTA CAACATGGTG TGGAGCACAG TTAAAGAAAC TAGAGCAGAG GGATATTTTA 
     2341 TTTCTATTCT TCAGCATCTT TTGCTGATTC GAAATGATTA TTTTATAAGG CAACAATACT 
     2401 TCAAATTAAT TGATGAGTGT GTATCCCAGA TTGTATTGCA TAGAGATGGA ATGGATCCAG 
     2461 ACTTCACATA TCGAAAAAGA CTAGATTTAG ATTTAACCCA GTTTGTAGAC ATTTGCATAG 
     2521 ATCAAGCAAA ACTAGAAGAG TTTGAAGAGA AAGCATCAGA ACTTTACAAG AAATTTGAAA 
     2581 AAGAGTTTAC CGACCACCAA GAAACTCAGG CTGAATTGCA GAAAAAAGAG GCAAAGATTA 
     2641 ATGAGCTTCA AGCAGAGCTA CAAGCTTTTA AGTCTCAGTT TGGTGCCTTG CCAGCTGATT 
     2701 GTAATATTCC TTTGCCTCCC TCTAAAGAAG GTGGAACTGG CCACTCAGCA CTTCCTCCTC 
     2761 CGCCTCCACT GCCTTCTGGT GGAGGGGTGC CGCCTCCACC TCCTCCCCCA CCACCTCCTC 
     2821 CACTTCCAGG AATGCGGATG CCATTCAGTG GTCCTGTGCC TCCACCACCT CCCCTGGGAT 
     2881 TCCTTGGAGG ACAAAATTCT CCTCCTCTAC CAATCCTGCC ATTTGGGTTG AAACCAAAGA 
     2941 AAGAATTTAA ACCTGAAATC AGCATGAGAA GATTGAATTG GTTAAAGATC AGACCTCATG 
     3001 AAATGACTGA AAACTGTTTC TGGATAAAAG TAAATGAAAA TAAGTATGAA AACGTGGATT 
     3061 TGCTTTGTAA ACTTGAGAAT ACATTTTGTT GCCAACAAAA AGAGAGAAGA GAAGAGGAAG 
     3121 ATATTGAAGA GAAGAAATCG ATTAAGAAAA AAATTAAAGA ACTTAAGTTT TTAGATTCTA 
     3181 AAATTGCCCA GAACCTTTCA ATCTTCCTGA GCTCTTTTCG GGTGCCATAT GAGGAAATCA 
     3241 GAATGATGAT ATTGGAAGTA GATGAAACAC GGTTGGCAGA GTCTATGATT CAGAACTTAA 
     3301 TAAAGCATCT TCCTGATCAA GAGCAATTAA ATTCATTGTC TCAGTTCAAG AGTGAATATA 
     3361 GCAACTTATG TGAACCTGAG CAGTTTGTGG TTGTGATGAG CAATGTGAAG AGACTACGGC 
     3421 CACGGCTCAG TGCTATTCTC TTTAAGCTTC AGTTTGAAGA GCAGGTGAAC AACATCAAAC 
     3481 CTGACATCAT GGCTGTCAGT ACTGCCTGCG AAGAGATAAA GAAGAGCAAA AGCTTTAGCA 
     3541 AGTTGCTGGA ACTTGTATTG CTAATGGGAA ACTACATGAA TGCTGGCTCC CGGAATGCTC 
     3601 AAACCTTCGG ATTTAACCTT AGCTCTCTCT GTAAACTAAA GGACACAAAA TCAGCAGATC 
     3661 AGAAAACAAC GCTACTTCAT TTCCTGGTAG AAATATGTGA AGAGAAGTAC CCTGATATAC 
     3721 TGAATTTTGT GGATGATTTG GAACCTTTAG ACAAAGCTAG TAAAGTCTCT GTAGAAACGC 
     3781 TGGAAAAGAA TTTGAGGCAG ATGGGAAGGC AGCTTCAACA GCTTGAGAAG GAATTGGAAA 
     3841 CCTTTCCCCC TCCTGAGGAC TTGCATGACA AGTTTGTGAC AAAGATGTCC AGATTTGTTA 
     3901 TCAGTGCAAA AGAACAATAT GAGACACTTT CGAAGTTACA CGAAAACATG GAAAAGTTAT 
     3961 ACCAGAGTAT AATAGGATAC TATGCCATTG ATGTGAAGAA GGTGTCTGTG GAAGACTTTC 
     4021 TTACTGACCT GAATAACTTC AGAACCACAT TCATGCAAGC AATAAAGGAG AATATCAAAA 
     4081 AAAGAGAAGC AGAGGAAAAA GAAAAACGTG TCAGAATAGC TAAAGAATTA GCAGAGCGAG 
     4141 AAAGACTCGA ACGCCAACAA AAGAAAAAGC GTTTATTAGA AATGAAGACT GAGGGTGATG 
     4201 AGACAGGAGT GATGGATAAT CTGCTGGAGG CCTTGCAGTC CGGGGCTGCC TTCCGCGACA 
     4261 GAAGAAAAAG GACACCGATG CCAAAAGATG TTCGGCAGAG TCTCAGTCCA ATGTCTCAGA 
     4321 GGCCTGTTCT GAAAGTTTGT AACCATGAAA ATCAGAAAGT GCAGTTAACA GAAGGGTCAC 
     4381 GTTCACACTA CAATATCAAT TGCAACTCAA CAAGGACTCC AGTCGCCAAG GAGCTTAATT 
     4441 ATAATCTAGA CACTCATACG TCTACTGGGA GGATCAAGGC AGCTGAGAAG AAGGAAGCGT 
     4501 GTAATGTAGA AAGCAACAGA AAAAAGGAAA CGGAACTTCT TGGCTCTTTT TCTAAAAATG 
     4561 AATCAGTTCC CGAAGTTGAA GCCCTGCTGG CAAGATTACG AGCTTTAACG CGTACGCGGC 
     4621 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4681 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4741 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4801 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4861 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4921 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4981 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5041 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5101 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5161 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5221 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5281 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5341 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5401 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5461 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5521 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5581 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5641 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5701 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5761 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5821 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5881 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5941 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6001 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6061 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6121 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6181 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6241 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6301 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6361 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6421 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6481 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6541 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6601 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6661 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6721 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6781 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6841 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6901 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6961 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7021 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7081 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7141 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7201 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7261 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7321 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7381 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7441 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7501 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7561 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7621 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7681 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7741 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7801 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7861 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7921 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7981 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8041 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8101 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8161 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8221 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8281 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8341 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8401 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//