LOCUS       RC221408                7419 bp    DNA     circular  SYN 
DEFINITION  CLOCK (Myc-DDK-tagged)-Human clock homolog (mouse) (CLOCK)
ACCESSION   NM_004898
VERSION     NM_004898.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3576..3667 
                     /label="Myc-DDK"  
     misc_feature    3697..4282 
                     /label="PolyA signal"  
     misc_feature    3754..3773 
                     /label="Restriction Enzyme"  
     misc_feature    4558..5230 
                     /label="ColE1"  
     misc_feature    5660..6454 
                     /label="Kan/Neo"  
     misc_feature    6489..6791 
                     /label="SV40 ori"  
     misc_feature    6877..7354 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3558..3564 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3558 
                     /label="CLOCK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGTTTACC GTAAGCTGTA GTAAAATGAG CTCGATTGTT GACAGAGATG 
     1081 ACAGTAGTAT TTTTGATGGG TTGGTGGAAG AAGATGACAA GGACAAAGCG AAAAGAGTAT 
     1141 CTAGAAACAA ATCTGAAAAG AAACGTAGAG ATCAATTTAA TGTTCTCATT AAAGAACTGG 
     1201 GATCCATGCT TCCTGGTAAT GCTAGAAAGA TGGACAAATC TACTGTTCTG CAGAAAAGCA 
     1261 TTGATTTTTT ACGAAAACAT AAAGAAATCA CTGCACAGTC AGATGCTAGT GAAATTCGAC 
     1321 AGGACTGGAA ACCTACATTC CTTAGTAATG AAGAGTTTAC ACAATTAATG TTAGAGGCTC 
     1381 TTGATGGTTT TTTTTTAGCA ATCATGACAG ATGGAAGCAT AATATATGTG TCTGAGAGTG 
     1441 TAACTTCATT ACTTGAACAT TTACCATCTG ATCTTGTGGA TCAAAGTATA TTTAATTTTA 
     1501 TCCCAGAAGG GGAACATTCA GAGGTTTATA AAATACTCTC TACTCATCTG CTGGAAAGTG 
     1561 ATTCATTAAC CCCAGAATAT TTAAAATCAA AAAATCAGTT AGAATTCTGT TGTCACATGC 
     1621 TGCGAGGAAC AATAGACCCA AAGGAGCCAT CTACCTATGA ATATGTAAAA TTTATAGGAA 
     1681 ATTTCAAATC TTTAAACAGT GTATCCTCTT CAGCACACAA TGGTTTTGAA GGAACTATAC 
     1741 AACGCACACA TAGGCCATCT TATGAAGATA GAGTTTGTTT TGTAGCTACT GTCAGGTTAG 
     1801 CTACACCTCA GTTCATCAAG GAAATGTGCA CTGTTGAAGA ACCCAATGAA GAGTTTACAT 
     1861 CTAGACATAG TTTAGAATGG AAGTTTCTGT TTCTAGATCA CAGGGCACCA CCCATAATAG 
     1921 GGTATTTGCC ATTTGAAGTT CTGGGAACAT CAGGCTATGA TTACTATCAT GTGGATGACC 
     1981 TAGAAAATTT GGCAAAATGT CATGAGCACT TAATGCAATA TGGGAAAGGC AAATCATGTT 
     2041 ATTATAGGTT CCTGACTAAG GGGCAACAGT GGATTTGGCT TCAGACTCAT TATTATATCA 
     2101 CTTACCATCA GTGGAATTCA AGGCCAGAGT TTATTGTTTG TACTCACACT GTAGTAAGTT 
     2161 ATGCAGAAGT TAGGGCTGAA AGACGACGAG AACTTGGCAT TGAAGAGTCT CTTCCTGAGA 
     2221 CAGCTGCTGA CAAAAGCCAA GATTCTGGGT CAGATAATCG TATAAACACA GTCAGTCTCA 
     2281 AGGAAGCATT GGAAAGGTTT GATCACAGCC CAACCCCTTC TGCCTCTTCT CGGAGTTCAA 
     2341 GAAAATCATC TCACACGGCC GTCTCAGACC CTTCCTCAAC ACCAACCAAG ATCCCGACGG 
     2401 ATACGAGCAC TCCACCCAGG CAGCATTTAC CAGCTCATGA GAAGATGGTG CAAAGAAGGT 
     2461 CATCATTTAG TAGTCAGTCC ATAAATTCCC AGTCTGTTGG TTCATCATTA ACACAGCCAG 
     2521 TGATGTCTCA AGCTACAAAT TTACCAATTC CACAAGGCAT GTCCCAGTTT CAGTTTTCAG 
     2581 CTCAATTAGG AGCCATGCAA CATCTGAAAG ACCAATTGGA ACAACGGACA CGCATGATAG 
     2641 AAGCAAATAT TCATCGGCAA CAAGAAGAAC TAAGAAAAAT TCAAGAACAA CTTCAGATGG 
     2701 TCCATGGTCA GGGGCTGCAG ATGTTTTTGC AACAATCAAA TCCTGGGTTG AATTTTGGTT 
     2761 CCGTTCAACT TTCTTCTGGA AATTCATCTA ACATCCAGCA ACTTGCACCT ATAAATATGC 
     2821 AAGGCCAAGT TGTTCCTACT AACCAGATTC AAAGTGGAAT GAATACTGGA CACATTGGCA 
     2881 CAACTCAGCA CATGATACAA CAACAGACTT TACAGAGTAC ATCAACTCAG AGTCAACAAA 
     2941 ATGTACTGAG TGGGCACAGT CAGCAAACAT CTCTACCCAG TCAGACACAG AGCACTCTTA 
     3001 CAGCCCCACT GTATAACACT ATGGTGATTT CTCAGCCTGC AGCCGGAAGC ATGGTCCAGA 
     3061 TTCCATCTAG TATGCCACAA AACAGCACCC AGAGTGCTGC AGTAACTACA TTCACTCAGG 
     3121 ACAGGCAGAT AAGATTTTCT CAAGGTCAAC AACTTGTGAC CAAATTAGTG ACTGCTCCTG 
     3181 TAGCTTGTGG GGCAGTCATG GTACCTAGTA CTATGCTTAT GGGCCAGGTG GTGACTGCAT 
     3241 ATCCTACTTT TGCTACACAA CAGCAACAGT CACAGACATT GTCAGTAACG CAGCAGCAGC 
     3301 AGCAGCAGAG CTCCCAGGAG CAGCAGCTCA CTTCAGTTCA GCAACCATCT CAGGCTCAGC 
     3361 TGACCCAGCC ACCGCAACAA TTTTTACAGA CTTCTAGGTT GCTCCATGGG AATCCCTCAA 
     3421 CTCAACTCAT TCTCTCTGCT GCATTTCCTC TACAACAGAG CACCTTCCCT CAGTCACATC 
     3481 ACCAGCAACA TCAGTCTCAG CAACAGCAGC AACTCAGCCG GCACAGGACT GACAGCTTGC 
     3541 CCGACCCTTC CAAGGTTCAA CCACAGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3601 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3661 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3721 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3781 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3841 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3901 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     3961 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4021 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4081 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4141 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4201 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4261 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4321 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4381 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4441 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4501 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4561 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4621 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4681 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4741 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4801 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4861 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     4921 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     4981 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5041 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5101 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5161 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5221 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5281 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5341 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5401 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5461 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5521 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5581 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5641 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5701 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5761 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     5821 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     5881 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     5941 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6001 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6061 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6121 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6181 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6241 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6301 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6361 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6421 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6481 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6541 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6601 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6661 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6721 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6781 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6841 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6901 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     6961 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7021 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7081 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7141 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7201 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7261 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7321 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7381 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//