LOCUS       RC221370                9168 bp    DNA     circular  SYN 
DEFINITION  ATP10D (Myc-DDK-tagged)-Human ATPase, class V, type 10D (ATP10D)
ACCESSION   NM_020453
VERSION     NM_020453.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5323..5414 
                     /label="Myc-DDK"  
     misc_feature    5444..6029 
                     /label="PolyA signal"  
     misc_feature    5501..5520 
                     /label="Restriction Enzyme"  
     misc_feature    6305..6977 
                     /label="ColE1"  
     misc_feature    7407..8201 
                     /label="Kan/Neo"  
     misc_feature    8236..8538 
                     /label="SV40 ori"  
     misc_feature    8624..9101 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5298..5305 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5298 
                     /label="ATP10D"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTGAGGCT CTCCAATGGG CCAGATATCA CTGGCGACGG CTGATCAGAG 
     1081 GTGCAACCAG GGATGATGAT TCAGGGCCAT ACAACTATTC CTCGTTGCTC GCCTGTGGGC 
     1141 GCAAGTCCTC TCAGACCCCT AAACTGTCAG GAAGGCACCG GATTGTTGTT CCCCACATCC 
     1201 AGCCCTTCAA GGATGAGTAT GAGAAGTTCT CCGGAGCCTA TGTGAACAAT CGAATACGAA 
     1261 CAACAAAGTA CACACTTCTG AATTTTGTGC CAAGAAATTT ATTTGAACAA TTTCACAGAG 
     1321 CTGCCAATTT ATATTTCCTG TTCCTAGTTG TCCTGAACTG GGTACCTTTG GTAGAAGCCT 
     1381 TCCAAAAGGA AATCACCATG TTGCCTCTGG TGGTGGTCCT TACAATTATC GCAATTAAAG 
     1441 ATGGCCTGGA AGGTTATCGG AAATACAAAA TTGACAAACA GATCAATAAT TTAATAACTA 
     1501 AAGTTTATAG TAGGAAAGAG AAAAAATACA TTGACCGATG CTGGAAAGAC GTTACTGTTG 
     1561 GGGACTTTAT TCGCCTCTCC TGCAACGAGG TCATCCCTGC AGACATGGTA CTACTCTTTT 
     1621 CCACTGATCC AGATGGAATC TGTCACATTG AGACTTCTGG TCTTGATGGA GAGAGCAATT 
     1681 TAAAACAGAG GCAGGTGGTT CGGGGATATG CAGAACAGGA CTCTGAAGTT GATCCTGAGA 
     1741 AGTTTTCCAG TAGGATAGAA TGTGAAAGCC CAAACAATGA CCTCAGCAGA TTCCGAGGCT 
     1801 TCCTAGAACA TTCCAACAAA GAACGCGTGG GTCTCAGTAA AGAAAATTTG TTGCTTAGAG 
     1861 GATGCACCAT TAGAAACACA GAGGCTGTTG TGGGCATTGT GGTTTATGCA GGCCATGAAA 
     1921 CCAAAGCAAT GCTGAACAAC AGTGGGCCAC GGTATAAGCG CAGCAAATTA GAAAGAAGAG 
     1981 CAAACACAGA TGTCCTCTGG TGTGTCATGC TTCTGGTCAT AATGTGCTTA ACTGGCGCAG 
     2041 TAGGTCATGG AATCTGGCTG AGCAGGTATG AAAAGATGCA TTTTTTCAAT GTTCCCGAGC 
     2101 CTGATGGACA TATCATATCA CCACTGTTGG CAGGATTTTA TATGTTTTGG ACCATGATCA 
     2161 TTTTGTTACA GGTCTTGATT CCTATTTCTC TCTATGTTTC CATCGAAATT GTGAAGCTTG 
     2221 GACAAATATA TTTCATTCAA AGTGATGTGG ATTTCTACAA TGAAAAAATG GATTCTATTG 
     2281 TTCAGTGCCG AGCCCTGAAC ATCGCCGAGG ATCTGGGACA GATTCAGTAC CTCTTTTCCG 
     2341 ATAAGACAGG AACCCTCACT GAGAATAAGA TGGTTTTTCG AAGATGTAGT GTGGCAGGAT 
     2401 TTGATTACTG CCATGAAGAA AATGCCAGGA GGTTGGAGTC CTATCAGGAA GCTGTCTCTG 
     2461 AAGATGAAGA TTTTATAGAC ACAGTCAGTG GTTCCCTCAG CAATATGGCA AAACCGAGAG 
     2521 CCCCCAGCTG CAGGACAGTT CATAATGGGC CTTTGGGAAA TAAGCCCTCA AATCATCTTG 
     2581 CTGGGAGCTC TTTTACTCTA GGAAGTGGAG AAGGAGCCAG TGAAGTGCCT CATTCCAGAC 
     2641 AGGCTGCTTT CAGTAGCCCC ATTGAAACAG ACGTGGTACC AGACACCAGG CTTTTAGACA 
     2701 AATTTAGTCA GATTACACCT CGGCTCTTTA TGCCACTAGA TGAGACCATC CAAAATCCAC 
     2761 CAATGGAAAC TTTGTACATT ATCGACTTTT TCATTGCATT GGCAATTTGC AACACAGTAG 
     2821 TGGTTTCTGC TCCTAACCAA CCCCGACAAA AGATCAGACA CCCTTCACTG GGGGGGTTGC 
     2881 CCATTAAGTC TTTGGAAGAG ATTAAAAGTC TTTTCCAGAG ATGGTCTGTC CGAAGATCAA 
     2941 GTTCTCCATC GCTTAACAGT GGGAAAGAGC CATCTTCTGG AGTTCCAAAC GCCTTTGTGA 
     3001 GCAGACTCCC TCTCTTTAGT CGAATGAAAC CAGCTTCACC TGTGGAGGAA GAGGTCTCCC 
     3061 AGGTGTGTGA GAGCCCCCAG TGCTCCAGTA GCTCAGCTTG CTGCACAGAA ACAGAGAAAC 
     3121 AACACGGTGA TGCAGGCCTC CTGAATGGCA AGGCAGAGTC CCTCCCTGGA CAGCCATTGG 
     3181 CCTGCAACCT GTGTTATGAG GCCGAGAGCC CAGACGAAGC GGCCTTAGTG TATGCCGCCA 
     3241 GGGCTTACCA ATGCACTTTA CGGTCTCGGA CACCAGAGCA GGTCATGGTG GACTTTGCTG 
     3301 CTTTGGGACC ATTAACATTT CAACTCCTAC ACATCCTGCC CTTTGACTCA GTAAGAAAAA 
     3361 GAATGTCTGT TGTGGTCCGA CACCCTCTTT CCAATCAAGT TGTGGTGTAT ACGAAAGGCG 
     3421 CTGATTCTGT GATCATGGAG TTACTGTCGG TGGCTTCCCC AGATGGAGCA AGTCTGGAGA 
     3481 AACAACAGAT GATAGTAAGG GAGAAAACCC AGAAGCACTT GGATGACTAT GCCAAACAAG 
     3541 GCCTTCGTAC TTTATGTATA GCAAAGAAGG TCATGAGTGA CACTGAATAT GCAGAGTGGC 
     3601 TGAGGAATCA TTTTTTAGCT GAAACCAGCA TTGACAACAG GGAAGAATTA CTACTTGAAT 
     3661 CTGCCATGAG GTTGGAGAAC AAACTTACAT TACTTGGTGC TACTGGCATT GAAGACCGTC 
     3721 TGCAGGAGGG AGTCCCTGAA TCTATAGAAG CTCTTCACAA AGCGGGCATC AAGATCTGGA 
     3781 TGCTGACAGG GGACAAGCAG GAGACAGCTG TCAACATAGC TTATGCATGC AAACTACTGG 
     3841 AGCCAGATGA CAAGCTTTTT ATCCTCAATA CCCAAAGTAA AGATGCCTGT GGGATGCTGA 
     3901 TGAGCACAAT TTTGAAAGAA CTTCAGAAGA AAACTCAAGC CCTGCCAGAG CAAGTGTCAT 
     3961 TAAGTGAAGA TTTACTTCAG CCTCCTGTCC CCCGGGACTC AGGGTTACGA GCTGGACTCA 
     4021 TTATCACTGG GAGGACCCTG GAGTTTGCCC TGCAAGAAAG TCTGCAAAAG CAGTTCCTGG 
     4081 AACTGACATC TTGGTGTCAA GCTGTGGTCT GCTGCCGAGC CACACCGCTG CAGAAAAGTG 
     4141 AAGTGGTGAA ATTGGTCCGC AGCCATCTCC AGGTGATGAC CCTTGCTATT GGTGATGGTG 
     4201 CCAATGATGT TAGCATGATA CAAGTGGCAG ACATTGGGAT AGGGGTCTCA GGTCAAGAAG 
     4261 GCATGCAGGC TGTGATGGCC AGTGACTTTG CCGTTTATCA GTTCAAACAT CTCAGCAAGC 
     4321 TCCTTCTTGT CCATGGACAC TGGTGTTATA CACGGCTTTC CAACATGATT CTCTATTTTT 
     4381 TCTATAAGAA TGTGGCCTAT GTGAACCTCC TTTTCTGGTA CCAGTTCTTT TGTGGATTTT 
     4441 CAGGGACATC CATGACTGAT TACTGGGTTT TGATCTTCTT CAACCTCCTC TTCACATCTG 
     4501 CCCCTCCTGT CATTTATGGT GTTTTGGAGA AAGATGTGTC TGCAGAGACC CTCATGCAAC 
     4561 TGCCTGAACT TTACAGAAGT GGTCAGAAAT CAGAGGCATA CTTACCCCAT ACCTTCTGGA 
     4621 TCACCTTATT GGATGCTTTT TATCAAAGCC TGGTCTGCTT CTTTGTGCCT TATTTTACCT 
     4681 ACCAGGGCTC AGATACTGAC ATCTTTGCAT TTGGAAACCC CCTGAACACA GCCGCTCTGT 
     4741 TCATCGTTCT CCTCCATCTG GTCATTGAAA GCAAGAGTTT GACTTGGATT CACTTGCTGG 
     4801 TCATCATTGG TAGCATCTTG TCTTATTTTT TATTTGCCAT AGTTTTTGGA GCCATGTGTG 
     4861 TAACTTGCAA CCCACCATCC AACCCTTACT GGATTATGCA GGAGCACATG CTGGATCCAG 
     4921 TATTCTACTT AGTTTGTATC CTCACGACGT CCATTGCTCT TCTGCCCAGG TTTGTATACA 
     4981 GAGTTCTTCA GGGATCCCTG TTTCCATCTC CAATTCTGAG AGCTAAGCAC TTTGACAGAC 
     5041 TAACTCCAGA GGAGAGGACT AAAGCTCTCA AGAAGTGGAG AGGGGCTGGA AAGATGAATC 
     5101 AAGTGACATC AAAGTATGCT AACCAATCAG CTGGCAAGTC AGGAAGAAGA CCCATGCCTG 
     5161 GCCCTTCTGC TGTATTTGCA ATGAAGTCAG CAAGTTCCTG TGCTATTGAG CAAGGAAACT 
     5221 TATCTCTGTG TGAGACTGCT TTAGATCAAG GCTACTCTGA AACTAAGGCC TTTGAGATGG 
     5281 CTGGACCCTC CAAAGGTAAA GAAAGCAGCG GACCGACGCG TACGCGGCCG CTCGAGCAGA 
     5341 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5401 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5461 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5521 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5581 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5641 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5701 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5761 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5821 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5881 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5941 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     6001 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     6061 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     6121 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     6181 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     6241 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     6301 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6361 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6421 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6481 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6541 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6601 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6661 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6721 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6781 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6841 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6901 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6961 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     7021 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     7081 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     7141 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     7201 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     7261 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     7321 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7381 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7441 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7501 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7561 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7621 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7681 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7741 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7801 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7861 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7921 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7981 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     8041 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     8101 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     8161 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     8221 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     8281 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     8341 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8401 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8461 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8521 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8581 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8641 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8701 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8761 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8821 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8881 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8941 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     9001 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     9061 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     9121 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//