LOCUS       RC221309L1                9045 bp    DNA     circular  SYN 
DEFINITION  PRKD3 (Myc-DDK-tagged)-Human protein kinase D3 (PRKD3)
ACCESSION   NM_005813
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7336..7516 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8244..8903 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7675..8243 
                     /label="ColE1"  
     misc_feature    6211..6231 
                     /label="LR50 primer"  
     misc_feature    6008..6079 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6220..6811 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5990..5996 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5990 
                     /label="PRKD3"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTCTGCAAAT AATTCCCCTC CATCAGCCCA 
     3361 GAAGTCTGTA TTACCCACAG CTATTCCTGC TGTGCTTCCA GCTGCTTCTC CGTGTTCAAG 
     3421 TCCTAAGACG GGACTCTCTG CCCGACTCTC TAATGGAAGC TTCAGTGCAC CATCACTCAC 
     3481 CAACTCCAGA GGCTCAGTGC ATACAGTTTC ATTTCTACTG CAAATTGGCC TCACACGGGA 
     3541 GAGTGTTACC ATTGAAGCCC AGGAACTGTC TTTATCTGCT GTCAAGGATC TTGTGTGCTC 
     3601 CATAGTTTAT CAAAAGTTTC CAGAGTGTGG ATTCTTTGGC ATGTATGACA AAATTCTTCT 
     3661 CTTTCGCCAT GACATGAACT CAGAAAACAT TTTGCAGCTG ATTACCTCAG CAGATGAAAT 
     3721 ACATGAAGGA GACCTAGTGG AAGTGGTTCT TTCAGCTTTA GCCACAGTAG AAGACTTCCA 
     3781 GATTCGTCCA CATACTCTCT ATGTACATTC TTACAAAGCT CCTACTTTCT GTGATTACTG 
     3841 TGGTGAGATG CTCTGGGGAT TGGTACGTCA AGGACTGAAA TGTGAAGGCT GTGGATTAAA 
     3901 TTACCATAAA CGATGTGCCT TCAAGATTCC AAATAACTGT AGTGGAGTAA GAAAGAGACG 
     3961 TCTGTCAAAT GTATCTTTAC CAGGACCCGG CCTCTCAGTT CCAAGACCCC TACAGCCTGA 
     4021 ATATGTAGCC CTTCCCAGTG AAGAGTCACA TGTCCACCAG GAACCAAGTA AGAGAATTCC 
     4081 TTCTTGGAGT GGTCGCCCAA TCTGGATGGA AAAGATGGTA ATGTGCAGAG TGAAAGTTCC 
     4141 ACACACATTT GCTGTTCACT CTTACACCCG TCCCACGATA TGTCAGTACT GCAAGCGGTT 
     4201 ACTGAAAGGC CTCTTTCGCC AAGGAATGCA GTGTAAAGAT TGCAAATTCA ACTGCCATAA 
     4261 ATGCTGTGCA TCAAAAGTAC CAAGAGACTG CCTTGGAGAG GTTACTTTCA ATGGAGAACC 
     4321 TTCCAGTCTG GGAACAGATA CAGATATACC AATGGATATT GACAATAATG ACATAAATAG 
     4381 TGATAGTAGT CGGGGTTTGG ATGACACAGA AGAGCCATCA CCCCCAGAAG ATAAGATGTT 
     4441 CTTCTTGGAC CCATCTGATC TCGATGTGGA AAGAGATGAA GAAGCCGTTA AAACAATCAG 
     4501 TCCATCAACA AGCAATAATA TTCCGCTCAT GAGGGTTGTA CAATCCATCA AGCACACAAA 
     4561 GAGGAAGAGC AGCACAATGG TGAAGGAAGG GTGGATGGTC CATTACACCA GCAGGGATAA 
     4621 CCTGAGAAAG AGGCATTATT GGAGACTTGA CAGCAAATGT CTAACATTAT TTCAGAATGA 
     4681 ATCTGGATCA AAGTATTATA AGGAAATTCC ACTTTCAGAA ATTCTCCGCA TATCTTCACC 
     4741 ACGAGATTTC ACAAACATTT CACAAGGCAG CAATCCACAC TGTTTTGAAA TCATTACTGA 
     4801 TACTATGGTA TACTTCGTTG GTGAGAACAA TGGGGACAGC TCTCATAATC CTGTTCTTGC 
     4861 TGCCACTGGA GTTGGACTTG ATGTAGCACA GAGCTGGGAA AAAGCAATTC GCCAAGCCCT 
     4921 CATGCCTGTT ACTCCTCAAG CAAGTGTTTG CACTTCTCCA GGGCAAGGGA AAGATCACAA 
     4981 AGATTTGTCT ACAAGTATCT CTGTATCTAA TTGTCAGATT CAGGAGAATG TGGATATCAG 
     5041 TACTGTTTAC CAGATCTTTG CAGATGAGGT GCTTGGTTCA GGCCAGTTTG GCATCGTTTA 
     5101 TGGAGGAAAA CATAGAAAGA CTGGGAGGGA TGTGGCTATT AAAGTAATTG ATAAGATGAG 
     5161 ATTCCCCACA AAACAAGAAA GTCAACTCCG TAATGAAGTG GCTATTTTAC AGAATTTGCA 
     5221 CCATCCTGGG ATTGTAAACC TGGAATGTAT GTTTGAAACC CCAGAACGAG TCTTTGTAGT 
     5281 AATGGAAAAG CTGCATGGAG ATATGTTGGA AATGATTCTA TCCAGTGAGA AAAGTCGGCT 
     5341 TCCAGAACGA ATTACTAAAT TCATGGTCAC ACAGATACTT GTTGCTTTGA GGAATCTGCA 
     5401 TTTTAAGAAT ATTGTGCACT GTGATTTAAA GCCAGAAAAT GTGCTGCTTG CATCAGCAGA 
     5461 GCCATTTCCT CAGGTGAAGC TGTGTGACTT TGGATTTGCA CGCATCATTG GTGAAAAGTC 
     5521 ATTCAGGAGA TCTGTGGTAG GAACTCCAGC ATACTTAGCC CCTGAAGTTC TCCGGAGCAA 
     5581 AGGTTACAAC CGTTCCCTAG ATATGTGGTC AGTGGGAGTT ATCATCTATG TGAGCCTCAG 
     5641 TGGCACATTT CCTTTTAATG AGGATGAAGA TATAAATGAC CAAATCCAAA ATGCTGCATT 
     5701 TATGTACCCA CCAAATCCAT GGAGAGAAAT TTCTGGTGAA GCAATTGATC TGATAAACAA 
     5761 TCTGCTTCAA GTGAAGATGA GAAAACGTTA CAGTGTTGAC AAATCTCTTA GTCATCCCTG 
     5821 GCTACAGGAC TATCAGACTT GGCTTGACCT TAGAGAATTT GAAACTCGCA TTGGAGAACG 
     5881 TTACATTACA CATGAAAGTG ATGATGCTCG CTGGGAAATA CATGCATACA CACATAACCT 
     5941 TGTATACCCA AAGCACTTCA TTATGGCTCC TAATCCAGAT GATATGGAAG AAGATCCTAC 
     6001 GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT 
     6061 CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC TGTACAAGTA 
     6121 GGATTCGTCG AGGGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATACATGTT 
     6181 TAAGGGTTCC GGTTCCACTA GGTACAATTC GATATCAAGC TTATCGATAA TCAACCTCTG 
     6241 GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA 
     6301 TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT 
     6361 TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC 
     6421 AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT 
     6481 GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG 
     6541 GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC 
     6601 AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC 
     6661 ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC 
     6721 CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT 
     6781 CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCATC GATACCGTCG ACCTCGATCG 
     6841 AGACCTAGAA AAACATGGAG CAATCACAAG TAGCAATACA GCAGCTACCA ATGCTGATTG 
     6901 TGCCTGGCTA GAAGCACAAG AGGAGGAGGA GGTGGGTTTT CCAGTCACAC CTCAGGTACC 
     6961 TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG 
     7021 GGGACTGGAA GGGCTAATTC ACTCCCAACG AAGACAAGAT ATCCTTGATC TGTGGATCTA 
     7081 CCACACACAA GGCTACTTCC CTGATTGGCA GAACTACACA CCAGGGCCAG GGATCAGATA 
     7141 TCCACTGACC TTTGGATGGT GCTACAAGCT AGTACCAGTT GAGCAAGAGA AGGTAGAAGA 
     7201 AGCCAATGAA GGAGAGAACA CCCGCTTGTT ACACCCTGTG AGCCTGCATG GGATGGATGA 
     7261 CCCGGAGAGA GAAGTATTAG AGTGGAGGTT TGACAGCCGC CTAGCATTTC ATCACATGGC 
     7321 CCGAGAGCTG CATCCGGACT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA 
     7381 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT 
     7441 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT 
     7501 TTAGTCAGTG TGGAAAATCT CTAGCAGCAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     7561 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     7621 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     7681 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     7741 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     7801 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     7861 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     7921 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     7981 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     8041 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     8101 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     8161 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     8221 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GATTACGCCC CGCCCTGCCA CTCATCGCAG 
     8281 TACTGTTGTA ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAAAC GGCATGATGA 
     8341 ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG TATAATATTT GCCCATGGTG 
     8401 AAAACGGGGG CGAAGAAGTT GTCCATATTG GCCACGTTTA AATCAAAACT GGTGAAACTC 
     8461 ACCCAGGGAT TGGCTGAGAC GAAAAACATA TTCTCAATAA ACCCTTTAGG GAAATAGGCC 
     8521 AGGTTTTCAC CGTAACACGC CACATCTTGC GAATATATGT GTAGAAACTG CCGGAAATCG 
     8581 TCGTGGTATT CACTCCAGAG CGATGAAAAC GTTTCAGTTT GCTCATGGAA AACGGTGTAA 
     8641 CAAGGGTGAA CACTATCCCA TATCACCAGC TCACCGTCTT TCATTGCCAT ACGGAACTCC 
     8701 GGATGAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG CCGGATAAAA CTTGTGCTTA 
     8761 TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA ATATCCAGCT GAACGGTCTG GTTATAGGTA 
     8821 CATTGAGCAA CTGACTGAAA TGCCTCAAAA TGTTCTTTAC GATGCCATTG GGATATATCA 
     8881 ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC ATACTCTTCC TTTTTCAATA TTATTGAAGC 
     8941 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     9001 CAAATAGGGG TCCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGAC
//