LOCUS       RC221259                9228 bp    DNA     circular  SYN 
DEFINITION  PTPRU (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, U (PTPRU), transcript variant 3
ACCESSION   NM_005704
VERSION     NM_005704.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5383..5474 
                     /label="Myc-DDK"  
     misc_feature    5504..6089 
                     /label="PolyA signal"  
     misc_feature    5561..5580 
                     /label="Restriction Enzyme"  
     misc_feature    6365..7037 
                     /label="ColE1"  
     misc_feature    7467..8261 
                     /label="Kan/Neo"  
     misc_feature    8296..8598 
                     /label="SV40 ori"  
     misc_feature    8684..9161 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5358..5365 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5358 
                     /label="PTPRU"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCGTGCC CAGGCGCTCG TGCTGGCACT CACCTTCCAG CTCTGCGCGC 
     1081 CGGAGACCGA GACTCCGGCA GCTGGCTGCA CCTTCGAGGA GGCAAGTGAC CCAGCAGTGC 
     1141 CCTGCGAGTA CAGCCAGGCC CAGTACGATG ACTTCCAGTG GGAGCAAGTG CGAATCCACC 
     1201 CTGGCACCCG GGCACCTGCG GACCTGCCCC ACGGCTCCTA CTTGATGGTC AACACTTCCC 
     1261 AGCATGCCCC AGGCCAGCGA GCCCATGTCA TCTTCCAGAG CCTGAGCGAG AATGATACCC 
     1321 ACTGTGTGCA GTTCAGCTAC TTCCTGTACA GCCGGGACGG GCACAGCCCG GGCACCCTGG 
     1381 GCGTCTACGT GCGCGTTAAT GGGGGCCCCC TGGGCAGTGC TGTGTGGAAT ATGACTGGAT 
     1441 CCCACGGCCG TCAGTGGCAC CAGGCTGAGC TGGCTGTCAG CACTTTCTGG CCCAATGAAT 
     1501 ATCAGGTGCT GTTTGAGGCC CTCATCTCCC CAGACCGCAG GGGCTACATG GGCCTAGATG 
     1561 ACATCCTGCT TCTCAGCTAC CCCTGCGCAA AGGCCCCACA CTTCTCCCGC CTGGGCGACG 
     1621 TGGAGGTCAA CGCGGGCCAG AACGCGTCGT TCCAGTGCAT GGCCGCGGGC AGAGCGGCCG 
     1681 AGGCCGAACG CTTCCTCTTG CAACGGCAGA GCGGGGCGCT GGTGCCGGCG GCGGGCGTGC 
     1741 GGCACATCAG CCACCGGCGC TTCCTGGCCA CTTTCCCGCT GGCTGCCGTG AGCCGCGCCG 
     1801 AGCAGGACCT GTACCGCTGT GTGTCCCAGG CCCCGCGCGG CGCGGGCGTC TCTAACTTCG 
     1861 CGGAGCTCAT CGTCAAGGAG CCCCCAACTC CCATCGCGCC CCCACAGCTG CTGCGTGCTG 
     1921 GCCCCACCTA CCTCATCATC CAGCTCAACA CCAACTCCAT CATTGGCGAC GGGCCGATCG 
     1981 TGCGCAAGGA GATTGAGTAC CGCATGGCGC GCGGGCCCTG GGCTGAGGTG CACGCCGTCA 
     2041 GCCTGCAGAC CTACAAGCTG TGGCACCTCG ACCCCGACAC AGAGTATGAG ATCAGCGTGC 
     2101 TGCTCACGCG TCCCGGAGAC GGCGGCACTG GCCGCCCTGG GCCACCCCTC ATCAGCCGCA 
     2161 CCAAATGCGC AGAGCCCATG AGGGCCCCCA AAGGCCTGGC TTTTGCTGAG ATCCAGGCCC 
     2221 GTCAGCTGAC CCTGCAGTGG GAACCACTGG GCTACAACGT GACGCGTTGC CACACCTATA 
     2281 CTGTGTCGCT GTGCTATCAC TACACCCTGG GCAGCAGCCA CAACCAGACC ATCCGAGAGT 
     2341 GTGTGAAGAC AGAGCAAGGT GTCAGCCGCT ACACCATCAA GAACCTGCTG CCCTATCGGA 
     2401 ACGTTCACGT GAGGCTTGTC CTCACTAACC CTGAGGGGCG CAAAGAGGGC AAGGAGGTCA 
     2461 CTTTCCAGAC GGATGAGGAT GTGCCCAGTG GGATTGCAGC CGAGTCCCTG ACCTTCACTC 
     2521 CACTGGAGGA CATGATCTTC CTCAAGTGGG AGGAGCCCCA GGAGCCCAAT GGTCTCATCA 
     2581 CCCAGTATGA GATCAGCTAC CAGAGCATCG AGTCATCAGA CCCGGCAGTG AACGTGCCAG 
     2641 GCCCACGACG TACCATCTCC AAGCTCCGCA ATGAGACCTA CCATGTCTTC TCCAACCTGC 
     2701 ACCCAGGCAC CACCTACCTG TTCTCCGTGC GGGCCCGCAC AGGCAAAGGC TTCGGCCAGG 
     2761 CGGCACTCAC TGAGATAACC ACTAACATCT CTGCTCCCAG CTTTGATTAT GCCGACATGC 
     2821 CGTCACCCCT GGGCGAGTCT GAGAACACCA TCACCGTGCT GCTGAGGCCG GCACAGGGCC 
     2881 GCGGTGCGCC CATCAGTGTG TACCAGGTGA TTGTGGAGGA GGAGCGGGCG CGGAGGCTGC 
     2941 GGCGGGAGCC AGGTGGACAG GACTGCTTCC CAGTGCCATT GACCTTCGAG GCGGCGCTGG 
     3001 CCCGAGGCCT GGTGCACTAC TTCGGGGCCG AACTGGCGGC CAGCAGTCTA CCTGAGGCCA 
     3061 TGCCCTTTAC CGTGGGTGAC AACCAGACCT ACCGAGGCTT CTGGAACCCA CCACTTGAGC 
     3121 CTAGGAAGGC CTATCTCATC TACTTCCAGG CAGCAAGCCA CCTGAAGGGG GAGACCCGGC 
     3181 TGAATTGCAT CCGCATTGCC AGGAAAGCTG CCTGCAAGGA AAGCAAGCGG CCCCTGGAGG 
     3241 TGTCCCAGAG ATCGGAGGAG ATGGGGCTTA TCCTGGGCAT CTGTGCAGGG GGGCTTGCTG 
     3301 TCCTCATCCT TCTCCTGGGT GCCATCATTG TCATCATCCG CAAAGGGAGA GACCACTATG 
     3361 CCTACTCCTA CTACCCGAAG CCGGTGAACA TGACCAAGGC CACCGTCAAC TACCGCCAGG 
     3421 AGAAGACACA CATGATGAGC GCCGTGGACC GCAGCTTCAC AGACCAGAGC ACCCTGCAGG 
     3481 AGGACGAGCG GCTGGGCCTG TCCTTCATGG ACACCCATGG CTACAGCACC CGGGGAGACC 
     3541 AGCGCAGCGG TGGGGTCACT GAGGCCAGCA GCCTCCTGGG GGGCTCCCCG AGGCGTCCCT 
     3601 GTGGCCGGAA GGGCTCCCCA TACCACACGG GGCAGCTGCA CCCTGCGGTG CGTGTCGCAG 
     3661 ACCTTCTGCA GCACATCAAC CAGATGAAGA CGGCCGAGGG TTACGGCTTC AAGCAGGAGT 
     3721 ATGAGAGCTT CTTTGAAGGC TGGGACGCCA CAAAGAAGAA AGACAAGGTC AAGGGCAGCC 
     3781 GGCAGGAGCC AATGCCTGCC TATGATCGGC ACCGAGTGAA ACTGCACCCG ATGCTGGGAG 
     3841 ACCCCAATGC CGACTACATT AATGCCAACT ACATAGATGG TTACCACAGG TCAAACCACT 
     3901 TCATAGCCAC TCAAGGGCCG AAGCCTGAGA TGGTCTATGA CTTCTGGCGT ATGGTGTGGC 
     3961 AGGAGCACTG TTCCAGCATC GTCATGATCA CCAAGCTGGT CGAGGTGGGC AGGGTGAAAT 
     4021 GCTCACGGTA CTGGCCGGAG GACTCAGACA CCTACGGGGA CATCAAGATT ATGCTGGTGA 
     4081 AGACAGAGAC CCTGGCTGAG TATGTCGTGC GCACTTTTGC CCTGGAGCGG AGAGGCTACT 
     4141 CTGCCCGGCA CGAGGTCCGC CAGTTCCACT TCACAGCGTG GCCAGAGCAT GGCGTCCCCT 
     4201 ACCATGCCAC GGGGCTGCTG GCTTTCATCC GGCGCGTGAA GGCCTCCACC CCACCTGATG 
     4261 CCGGGCCCAT TGTCATCCAC TGCAGCGCGG GCACCGGCCG CACAGGTTGC TATATCGTCC 
     4321 TGGATGTGAT GCTGGACATG GCAGAGTGTG AGGGCGTCGT GGACATTTAC AACTGTGTGA 
     4381 AGACTCTCTG CTCCCGGCGT GTCAACATGA TCCAGACTGA GGAGCAGTAC ATCTTCATTC 
     4441 ATGATGCAAT CCTGGAGGCC TGCCTGTGTG GGGAGACCAC CATCCCTGTC AGTGAGTTCA 
     4501 AGGCCACCTA CAAGGAGATG ATCCGCATTG ATCCTCAGAG TAATTCCTCC CAGCTGCGGG 
     4561 AAGAGTTCCA GACGCTGAAC TCGGTCACCC CGCCGCTGGA CGTGGAGGAG TGCAGCATCG 
     4621 CCCTGTTGCC CCGGAACCGC GACAAGAACC GCAGCATGGA CGTCCTGCCG CCCGACCGCT 
     4681 GCCTGCCCTT CCTCATCTCC ACTGATGGGG ACTCCAACAA CTACATTAAT GCAGCCCTGA 
     4741 CTGACAGCTA CACACGGAGT GCGGCCTTCA TCGTGACCCT GCACCCGCTG CAGAGCACCA 
     4801 CGCCCGACTT CTGGCGGCTG GTCTACGATT ACGGGTGCAC CTCCATCGTC ATGCTCAACC 
     4861 AGCTGAACCA GTCCAACTCC GCCTGGCCCT GCCTGCAGTA CTGGCCAGAG CCAGGCCGGC 
     4921 AGCAATATGG CCTCATGGAG GTGGAGTTTA TGTCGGGCAC AGCTGATGAA GACTTAGTGG 
     4981 CTCGAGTCTT CCGGGTGCAG AACATCTCTC GGTTGCAGGA GGGGCACCTG CTGGTGCGGC 
     5041 ACTTCCAGTT CCTGCGCTGG TCTGCATACC GGGACACACC TGACTCCAAG AAGGCCTTCT 
     5101 TGCACCTGCT GGCTGAGGTG GACAAGTGGC AGGCCGAGAG TGGGGATGGG CGCACCATCG 
     5161 TGCACTGCCT AAACGGGGGA GGACGCAGCG GCACCTTCTG CGCCTGCGCC ACGGTCCTGG 
     5221 AGATGATCCG CTGCCACAAC TTGGTGGACG TTTTCTTTGC TGCCAAAACC CTCCGGAACT 
     5281 ACAAACCCAA CATGGTGGAG ACCATGGATC AGTACCACTT TTGCTACGAT GTGGCCCTGG 
     5341 AGTACTTGGA GGGGCTGGAG TCAAGAAGCG GACCGACGCG TACGCGGCCG CTCGAGCAGA 
     5401 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5461 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5521 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5581 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5641 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5701 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5761 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5821 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5881 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5941 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     6001 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     6061 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     6121 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     6181 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     6241 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     6301 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     6361 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6421 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6481 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6541 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6601 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6661 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6721 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6781 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6841 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6901 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6961 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     7021 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     7081 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     7141 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     7201 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     7261 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     7321 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     7381 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7441 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7501 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7561 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7621 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7681 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7741 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7801 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7861 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7921 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7981 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     8041 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     8101 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     8161 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     8221 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     8281 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     8341 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     8401 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8461 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8521 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8581 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8641 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8701 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8761 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8821 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8881 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8941 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     9001 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     9061 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     9121 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     9181 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//