LOCUS       RC221241                6933 bp    DNA     circular  SYN 
DEFINITION  FCHSD2 (Myc-DDK-tagged)-Human FCH and double SH3 domains 2 (FCHSD2)
ACCESSION   NM_014824
VERSION     NM_014824.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3090..3181 
                     /label="Myc-DDK"  
     misc_feature    3211..3796 
                     /label="PolyA signal"  
     misc_feature    3268..3287 
                     /label="Restriction Enzyme"  
     misc_feature    4072..4744 
                     /label="ColE1"  
     misc_feature    5174..5968 
                     /label="Kan/Neo"  
     misc_feature    6003..6305 
                     /label="SV40 ori"  
     misc_feature    6391..6868 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3072..3078 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3072 
                     /label="FCHSD2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGAAGTTG GCTAGTCAAT ACCTGAAGAG AGATTGGCCT GGAGTAAAAG 
     1081 CTGATGATCG GAATGATTAC AGGAGCATGT ATCCCGTTTG GAAATCTTTT CTCGAGGGAA 
     1141 CAATGCAGGT AGCCCAGTCT CGGATGAATA TATGTGAAAA CTATAAAAAC TTCATTTCTG 
     1201 AGCCTGCAAG GACAGTGAGA AGCTTAAAAG AACAGCAACT AAAAAGGTGT GTGGACCAGT 
     1261 TGACAAAGAT CCAAACTGAA TTACAAGAGA CAGTGAAAGA TTTAGCTAAA GGCAAAAAGA 
     1321 AATACTTTGA GACTGAACAG ATGGCTCATG CAGTACGAGA GAAAGCTGAC ATCGAGGCAA 
     1381 AATCTAAACT TAGTCTTTTT CAATCAAGAA TCAGTTTACA GAAGGCAAGT GTAAAGTTAA 
     1441 AAGCCCGGCG ATCTGAGTGT AATTCCAAAG CTACCCACGC AAGGAATGAT TATCTTCTTA 
     1501 CCCTAGCGGC AGCAAATGCA CATCAGGATC GCTACTATCA AACAGATTTA GTTAACATTA 
     1561 TGAAGGCTCT TGATGGAAAT GTGTATGATC ATCTCAAGGA TTATTTAATA GCCTTCAGCC 
     1621 GGACTGAGCT AGAAACATGC CAAGCTGTGC AGAACACATT CCAGTTTTTA TTAGAAAACT 
     1681 CCAGCAAGGT GGTCCGGGAC TACAATCTTC AGCTGTTTTT GCAAGAAAAC GCTGTATTTC 
     1741 ACAAACCCCA GCCCTTCCAG TTCCAGCCTT GTGACAGTGA TACTAGCCGA CAGTTAGAAT 
     1801 CAGAAACTGG GACCACAGAG GAGCACAGTC TAAATAAGGA AGCTCGAAAA TGGGCCACAC 
     1861 GTGTGGCACG TGAGCATAAA AACATTGTTC ACCAACAACG GGTTCTAAAT GATCTGGAGT 
     1921 GTCATGGAGC TGCTGTATCA GAACAAAGCC GAGCAGAGCT AGAACAGAAA ATAGATGAAG 
     1981 CTAGAGAAAA TATTCGTAAA GCAGAGATAA TTAAATTGAA AGCTGAAGCC CGGTTGGACC 
     2041 TGCTAAAGCA GATTGGTGTT TCTGTGGACA CATGGCTAAA GAGTGCCATG AACCAAGTAA 
     2101 TGGAAGAACT GGAAAATGAG CGATGGGCCC GCCCTCCTGC AGTGACCAGT AATGGCACTT 
     2161 TACACTCGCT TAATGCAGAT ACCGAAAGAG AAGAAGGCGA AGAGTTTGAA GATAACATGG 
     2221 ATGTTTTCGA TGACAGCAGT TCCAGCCCTT CTGGCACCTT AAGAAATTAT CCACTCACCT 
     2281 GCAAAGTTGT TTATTCCTAC AAGGCTTCTC AACCAGATGA GTTGACCATT GAGGAACATG 
     2341 AGGTGTTAGA AGTGATTGAA GATGGAGATA TGGAAGACTG GGTAAAGGCT CGAAATAAAG 
     2401 TTGGCCAAGT GGGTTATGTG CCAGAAAAGT ACCTACAGTT TCCCACCTCG AACAGCCTCC 
     2461 TGAGCATGCT GCAGTCCCTG GCCGCTTTGG ACAGTCGGTC ACACACGTCC AGCAATTCCA 
     2521 CGGAAGCAGA ACTCGTTTCA GGCAGCCTCA ACGGAGATGC CAGTGTATGT TTTGTGAAAG 
     2581 CACTTTATGA TTATGAGGGC CAGACAGATG ATGAGTTATC TTTTCCTGAG GGAGCAATAA 
     2641 TCCGTATCTT GAACAAAGAA AACCAAGATG ATGATGGCTT CTGGGAAGGG GAATTCAATG 
     2701 GGCGTATTGG AGTTTTCCCA TCGGTGCTAG TGGAAGAACT TTCAGCCTCA GAAAATGGTG 
     2761 ACACTCCATG GATGAGAGAG ATTCAGATCT CTCCTTCCCC CAAGCCACAC GCCTCCCTGC 
     2821 CTCCACTGCC GTTGTACGAC CAGCCTCCCA GCAGCCCCTA CCCCAGCCCA GATAAGAGGA 
     2881 GCTCCCTGTA CTTTCCCCGG TCTCCTTCAG CAAACGAAAA AAGCCTTCAT GCTGAGTCAC 
     2941 CAGGATTCTC ACAGGCATCA AGGCATACTC CTGAGACCTC ATATGGCAAA CTGCGACCTG 
     3001 TCCGGGCAGC TCCCCCTCCA CCTACACAGA ATCACCGAAG GCCAGCAGAG AAGATTGAAG 
     3061 ATGTGGAAAT CACACTGGTG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3121 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3181 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3241 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3301 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3361 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3421 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3481 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3541 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3601 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     3661 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     3721 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     3781 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     3841 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     3901 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     3961 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4021 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4081 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4141 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4201 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4261 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4321 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4381 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4441 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4501 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4561 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4621 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     4681 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     4741 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     4801 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     4861 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     4921 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     4981 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5041 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5101 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5161 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5221 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5281 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5341 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5401 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5461 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5521 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5581 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     5641 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     5701 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     5761 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     5821 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     5881 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     5941 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6001 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6061 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6121 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6181 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6241 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6301 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6361 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6421 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6481 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6541 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6601 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     6661 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     6721 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     6781 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     6841 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     6901 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//