LOCUS       RC221231                8334 bp    DNA     circular  SYN 
DEFINITION  NRD1 (Myc-DDK-tagged)-Human nardilysin (N-arginine dibasic convertase) (NRD1), transcript variant 2
ACCESSION   NM_001101662
VERSION     NM_001101662.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4491..4582 
                     /label="Myc-DDK"  
     misc_feature    4612..5197 
                     /label="PolyA signal"  
     misc_feature    4669..4688 
                     /label="Restriction Enzyme"  
     misc_feature    5473..6145 
                     /label="ColE1"  
     misc_feature    6575..7369 
                     /label="Kan/Neo"  
     misc_feature    7404..7706 
                     /label="SV40 ori"  
     misc_feature    7792..8269 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4473..4479 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4473 
                     /label="NRDC"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGAGGAGA GTCACTGTTG CTGCAGTCTG TGCCACCCGG AGGAAGTTGT 
     1081 GTGAGGCCGG GCGGGAGCTC GCGGCGCTCT GGGGAATCGA AACGCGGGGT CGGTGCGAAG 
     1141 ACTCTGCTGC TGCCAGACCC TTTCCTATTC TGGCCATGCC TGGAAGGAAC AAGGCGAAGT 
     1201 CTACCTGCAG CTGCCCTGAC CTGCAGCCCA ATGGACAGGA TCTGGGCGAG AACAGCCGGG 
     1261 TTGCCCGTCT AGGAGCGGAT GAATCTGAGG AAGAGGGACG GAGGGGGTCT CTCAGTAATG 
     1321 CTGGGGACCC TGAGATCGTC AAGTCTCCCA GCGACCCCAA GCAATACCGA TACATCAAAT 
     1381 TACAGAATGG CTTGCAGGCA CTTCTGATTT CAGACCTAAG TAATATGGAA GGTAAAACAG 
     1441 GAAATACAAC AGATGATGAA GAAGAAGAGG AGGTGGAGGA AGAAGAAGAA GATGATGATG 
     1501 AAGATTCTGG AGCTGAAATA GAAGATGACG ATGAAGAGGG TTTTGATGAT GAAGATGAGT 
     1561 TTGATGATGA ACATGATGAT GATCTTGATA CTGAGGATAA TGAATTGGAA GAATTAGAAG 
     1621 AGAGAGCAGA AGCTAGAAAA AAAACTACTG AAAAACAGTC TGCAGCGGCT CTTTGTGTTG 
     1681 GAGTTGGGAG TTTCGCTGAT CCAGATGACC TGCCGGGGCT GGCACACTTT TTGGAGCACA 
     1741 TGGTATTCAT GGGTAGTTTG AAATATCCAG ATGAGAATGG ATTTGATGCC TTCCTGAAGA 
     1801 AGCATGGGGG TAGTGATAAT GCCTCAACTG ATTGTGAACG CACTGTCTTT CAGTTTGATG 
     1861 TCCAGAGGAA GTACTTCAAG GAAGCTCTTG ATAGATGGGC GCAGTTCTTC ATCCACCCAC 
     1921 TAATGATCAG AGATGCAATT GACCGTGAAG TTGAAGCTGT TGATAGTGAA TATCAACTTG 
     1981 CAAGGCCTTC TGATGCAAAC AGAAAGGAAA TGTTGTTTGG AAGCCTTGCT AGACCTGGAC 
     2041 ATCCTATGGG AAAATTTTTT TGGGGAAATG CTGAGACGCT CAAGCATGAG CCAAGAAAGA 
     2101 ATAATATTGA TACACATGCT AGATTGAGAG AATTCTGGAT GCGTTACTAC TCTTCTCATT 
     2161 ACATGACTTT AGTGGTTCAA TCCAAAGAAA CACTGGATAC TTTGGAAAAG TGGGTGACTG 
     2221 AAATCTTCTC TCAGATACCA AACAATGGGT TACCCAGACC AAACTTTGGC CATTTAACGG 
     2281 ATCCATTTGA CACACCAGCA TTTAACAAAC TTTATAGAGT TGTTCCAATC AGAAAAATTC 
     2341 ATGCTCTGAC CATCACATGG GCACTTCCTC CTCAACAGCA ACATTACAGG GTGAAGCCAC 
     2401 TTCATTATAT ATCCTGGCTG GTTGGACATG AAGGCAAAGG CAGCATTCTT TCTTTCCTTA 
     2461 GGAAAAAATG CTGGGCTCTT GCACTGTTTG GTGGAAATGG TGAGACAGGA TTTGAGCAAA 
     2521 ATTCTACTTA TTCAGTGTTC AGCATTTCTA TTACATTGAC TGATGAGGGT TATGAACATT 
     2581 TTTATGAGGT TGCTTACACT GTCTTTCAGT ATTTAAAAAT GCTGCAGAAG CTAGGCCCAG 
     2641 AAAAAAGAAT TTTTGAAGAG ATTCGGAAAA TTGAGGATAA TGAATTTCAT TACCAAGAAC 
     2701 AGACAGATCC AGTTGAGTAT GTGGAAAACA TGTGTGAGAA CATGCAGCTG TACCCATTGC 
     2761 AGGACATTCT CACTGGAGAT CAGCTTCTTT TTGAATACAA GCCAGAAGTC ATTGGTGAAG 
     2821 CCTTGAATCA GCTAGTTCCT CAAAAAGCAA ATCTTGTTTT ACTGTCTGGT GCTAATGAGG 
     2881 GAAAATGTGA CCTCAAGGAG AAATGGTTTG GAACTCAATA TAGTATAGAA GATATTGAAA 
     2941 ACTCTTGGGC TGAACTGTGG AATAGTAATT TCGAATTAAA TCCAGATCTT CATCTTCCAG 
     3001 CTGAAAACAA GTACATAGCC ACGGACTTTA CGTTGAAGGC TTTCGATTGC CCGGAAACAG 
     3061 AATACCCAGT TAAAATTGTG AATACTCCAC AAGGTTGCCT GTGGTATAAG AAAGACAACA 
     3121 AATTCAAAAT CCCCAAAGCA TATATACGTT TCCATCTAAT TTCACCGTTG ATACAGAAAT 
     3181 CTGCAGCAAA TGTGGTCCTC TTTGATATCT TTGTCAATAT CCTTACGCAT AACCTTGCGG 
     3241 AACCAGCTTA TGAAGCAGAT GTGGCACAGC TGGAGTATAA ACTGGTAGCT GGAGAACATG 
     3301 GTTTAATTAT TCGAGTGAAA GGATTTAACC ACAAACTACC TCTACTGTTT CAGCTCATTA 
     3361 TTGACTACTT AGCTGAGTTC AATTCCACAC CAGCTGTCTT TACAATGATA ACTGAGCAGT 
     3421 TGAAGAAGAC CTACTTTAAC ATCCTCATCA AGCCCGAGAC TTTGGCCAAA GATGTACGGC 
     3481 TTTTAATCTT GGAATATGCC CGTTGGTCTA TGATTGACAA GTACCAGGCT TTGATGGACG 
     3541 GCCTTTCCCT TGAGTCTCTG CTGAGCTTCG TCAAAGAATT CAAATCCCAG CTCTTTGTGG 
     3601 AGGGCCTGGT ACAAGGGAAT GTCACAAGCA CAGAATCTAT GGATTTCCTG AAATATGTTG 
     3661 TTGACAAACT AAACTTCAAG CCTCTGGAGC AGGAGATGCC TGTGCAGTTC CAGGTGGTAG 
     3721 AGCTGCCCAG TGGCCACCAT CTATGCAAAG TGAAAGCTCT GAACAAGGGT GATGCCAACT 
     3781 CTGAAGTCAC TGTGTACTAC CAGTCAGGTA CCAGGAGTCT AAGAGAATAT ACGCTTATGG 
     3841 AGCTGCTTGT GATGCACATG GAAGAACCTT GTTTTGACTT CCTTCGAACC AAGCAGACCC 
     3901 TTGGGTACCA TGTCTACCCT ACCTGTAGGA ACACATCCGG GATTCTAGGA TTTTCTGTCA 
     3961 CTGTGGGGAC TCAGGCAACC AAATACAATT CTGAAGTTGT TGATAAGAAG ATAGAAGAGT 
     4021 TTCTTTCTAG CTTTGAGGAG AAGATTGAGA ACCTCACTGA AGAGGCATTC AACACCCAGG 
     4081 TCACAGCTCT CATCAAGCTG AAGGAGTGTG AGGATACCCA CCTTGGGGAG GAGGTGGATA 
     4141 GGAACTGGAA TGAAGTGGTT ACACAGCAGT ACCTCTTTGA CCGCCTTGCC CACGAGATTG 
     4201 AAGCACTGAA GTCATTCTCA AAATCAGACC TGGTCAACTG GTTCAAGGCC CATAGAGGGC 
     4261 CAGGAAGTAA AATGCTCAGC GTTCATGTTG TTGGATATGG GAAGTATGAA CTGGAAGAGG 
     4321 ATGGTACCCC TTCTAGTGAG GATTCAAATT CTTCTTGTGA AGTGATGCAG CTGACCTACC 
     4381 TGCCAACCTC TCCTCTGCTG GCAGATTGTA TCATCCCCAT TACTGATATC AGGGCTTTCA 
     4441 CAACAACACT CAACCTTCTC CCCTACCATA AAATAGTCAA AACGCGTACG CGGCCGCTCG 
     4501 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4561 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4621 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4681 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4741 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4801 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4861 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4921 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4981 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5041 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5101 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5161 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5221 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5281 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5341 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5401 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5461 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5521 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5581 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5641 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5701 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5761 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5821 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5881 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5941 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6001 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6061 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6121 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6181 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6241 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6301 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6361 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6421 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6481 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6541 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6601 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6661 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6721 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6781 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6841 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6901 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6961 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7021 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7081 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7141 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7201 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7261 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7321 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7381 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7441 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7501 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7561 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7621 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7681 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7741 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7801 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7861 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7921 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7981 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8041 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8101 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8161 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8221 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8281 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//