LOCUS       RC221215                9198 bp    DNA     circular  SYN 
DEFINITION  FHOD3 (Myc-DDK-tagged)-Human formin homology 2 domain containing 3 (FHOD3)
ACCESSION   NM_025135
VERSION     NM_025135.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5355..5446 
                     /label="Myc-DDK"  
     misc_feature    5476..6061 
                     /label="PolyA signal"  
     misc_feature    5533..5552 
                     /label="Restriction Enzyme"  
     misc_feature    6337..7009 
                     /label="ColE1"  
     misc_feature    7439..8233 
                     /label="Kan/Neo"  
     misc_feature    8268..8570 
                     /label="SV40 ori"  
     misc_feature    8656..9133 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5337..5343 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5337 
                     /label="FHOD3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCACGCTG GCTTGCCGGG TGCAGTTCTT GGACGACACG GACCCTTTCA 
     1081 ACAGCACCAA CTTCCCCGAG CCCAGCCGGC CGCCGCTGTT CACGTTCCGC GAGGACCTCG 
     1141 CGCTCGGCAC CCAGCTGGCG GGGGTCCATA GGCTGCTGCA GGCGCCGCAC AAGCTGGATG 
     1201 ACTGTACTCT GCAGCTCTCT CACAATGGCG CCTACCTGGA TTTGGAGGCC ACCCTGGCAG 
     1261 AGCAGCGGGA TGAGTTGGAA GGCTTCCAGG ATGACGCCGG GCGGGGCAAG AAGCACAGCA 
     1321 TCATCCTAAG GACGCAGCTG TCTGTGAGGG TCCATGCCTG CATCGAAAAA CTATACAACT 
     1381 CCAGCGGACG AGATTTGAGA AGGGCCCTCT TCTCCCTGAA GCAGATATTT CAGGATGACA 
     1441 AGGATTTGGT GCATGAATTT GTAGTGGCTG AAGGTCTGAC ATGTTTGATC AAGGTGGGAG 
     1501 CTGAGGCTGA TCAGAACTAT CAGAACTACA TCTTAAGGGC TTTGGGCCAG ATTATGTTGT 
     1561 ATGTGGATGG AATGAATGGA GTAATAAACC GCAATGAAAC CATTCAGTGG CTGTACACTC 
     1621 TCATTGGGTC AAAGTTCCGC CTGGTGGTGA AGACAGCCCT GAAGCTGCTG CTCGTCTTTG 
     1681 TAGAGTACTC GGAGTCCAAC GCACCTCTCC TAATTCAGGC TGTCACTGCT GTTGACACGA 
     1741 AAAGAGGGGT CAAACCTTGG TCAAATATCA TGGAAATCCT GGAGGAAAAA GATGGAGTTG 
     1801 ATACGGAGCT ACTGGTTTAT GCAATGACTT TGGTGAACAA GACGTTATCA GGACTACCAG 
     1861 ACCAAGACAC CTTCTACGAC GTCGTGGACT GCCTGGAGGA GCTGGGCATT GCTGCTGTGT 
     1921 CCCAGAGGCA CTTGAACAAG AAAGGGACTG ACCTGGACTT AGTGGAGCAA CTCAACATTT 
     1981 ATGAGGTGGC GCTCAGGCAC GAGGATGGCG ATGAGACCAC GGAGCCACCC CCCAGTGGGT 
     2041 GCCGGGACCG GAGGAGGGCC AGCGTGTGTT CCAGTGGCGG AGGCGAGCAC CGGGGCCTGG 
     2101 ACCGCAGAAG GAGCCGCAGG CACTCGGTGC AGAGCATCAA GAGCACCCTG TCGGCCCCCA 
     2161 CCAGTCCCTG CTCCCAGTCA GCTCCCAGCT TCAAGCCCAA CCAAGTGCGA GATCTGCGTG 
     2221 AAAAATACAG CAATTTTGGC AATAACTCTT ATCACTCCTC AAGACCCTCA TCTGGATCCA 
     2281 GTGTGCCCAC CACCCCCACA TCATCCGTCT CACCCCCACA GGAGGCCAGG TTGGAAAGGT 
     2341 CATCACCGAG TGGTCTTCTC ACATCATCCT TCAGGCAGCA CCAAGAGTCA CTGGCAGCAG 
     2401 AGAGAGAGAG GCGGCGGCAG GAGAGAGAAG AAAGGTTGCA GAGAATAGAG CGGGAAGAAA 
     2461 GAAACAAATT CAGCCGAGAT TATTTAGACA AAAGAGAGGA GCAAAGGCAA GCAAGAGAAG 
     2521 AAAGATACAA ATACTTGGAA CAGTTGGCAG CTGAGGAGCA CGAGAAGGAG CTGAGAAGCC 
     2581 GGAGTGTGAG CCGGGGCAGA GCCGACCTCT CCTTGGACCT GACCTCGCCA GCAGCCCCAG 
     2641 CCTGCCTGGC TCCTCTGAGC CATAGCCCCT CATCTTCAGA CTCTCAAGAG GCTCTCACGG 
     2701 TGTCTGCCTC CTCCCCAGGA ACCCCTCACC ATCCCCAAGC AAGTGCCGGG GATCCTGAAC 
     2761 CCGAATCAGA GGCAGAACCG GAAGCAGAGG CAGGGGCGGG GCAGGTTGCT GATGAAGCTG 
     2821 GCCAGGACAT AGCCTCTGCC CACGAGGGTG CAGAGACTGA AGTGGAGCAG GCACTAGAGC 
     2881 AAGAGCCGGA AGAAAGAGCC TCCCTCAGTG AAAAAGAGAG GCAGAACGAG GGGGTGAACG 
     2941 AGAGGGACAA CTGCTCTGCC TCCAGCGTCT CGTCCTCCAG CAGCACGTTG GAGAGGGAGG 
     3001 AGAAGGAGGA CAAGCTCTCC AGGGACAGGA CAACTGGTTT GTGGCCCGCA GGTGTCCAGG 
     3061 ATGCAGGTGT AAATGGACAG TGTGGCGACA TCCTCACCAA CAAACGGTTC ATGCTTGACA 
     3121 TGCTGTATGC CCATAACAGG AAGTCTCCGG ATGATGAGGA GAAGGGGGAT GGGGAGGCTG 
     3181 GGAGGACCCA GCAGGAGGCA GAGGCGGTAG CCAGCCTTGC TACCAGGATA TCCACCCTGC 
     3241 AGGCCAACTC TCAGACCCAG GATGAGAGTG TCAGGAGGGT GGATGTCGGC TGTTTGGACA 
     3301 ATCGGGGCAG TGTGAAAGCA TTTGCTGAGA AATTCAACAG TGGGGACCTG GGGAGAGGTT 
     3361 CCATCTCCCC TGATGCTGAG CCCAATGACA AGGTCCCAGA AACAGCGCCG GTGCAGCCGA 
     3421 AGACAGAGTC TGATTACATC TGGGACCAGC TCATGGCCAA TCCAAGAGAG CTCAGAATCC 
     3481 AAGACATGGA TTTCACTGAC CTGGGGGAGG AGGATGACAT TGATGTCCTA GATGTGGACC 
     3541 TGGGTCACAG GGAGGCCCCT GGGCCACCTC CCCCACCCCC ACCCACCTTT CTGGGTTTGC 
     3601 CGCCCCCACC CCCTCCGCCC CTGTTGGACA GCATTCCTCC CCCTCCTGTC CCTGGTAATT 
     3661 TATTGGTTCC TCCTCCTCCA GTGTTCAACG CTCCTCAGGG CTTAGGGTGG TCCCAGGTAC 
     3721 CCAGGGGTCA GCCCACATTC ACTAAGAAAA AGAAGACCAT CCGTTTGTTC TGGAATGAAG 
     3781 TTCGGCCTTT TGACTGGCCA TGTAAAAACA ACCGACGCTG CAGAGAATTC CTGTGGTCAA 
     3841 AACTGGAACC CATTAAGGTG GACACTTCCA GACTGGAGCA CCTGTTTGAG TCTAAATCCA 
     3901 AGGAACTGTC TGTCTCAAAG AAAACTGCTG CAGATGGAAA AAGGCAAGAG ATCATTGTTC 
     3961 TGGATTCCAA GAGGAGTAAC GCCATCAATA TTGGTCTGAC GGTGCTGCCC CCTCCAAGGA 
     4021 CGATTAAGAT CGCCATTTTG AATTTTGATG AGTATGCCTT AAACAAAGAA GGAATCGAGA 
     4081 AAATTCTAAC GATGATTCCC ACCGATGAGG AGAAGCAGAA AATCCAGGAA GCTCAGCTGG 
     4141 CCAACCCTGA AATCCCCCTG GGCAGTGCAG AGCAGTTCCT CCTCACCCTG TCCTCCATCA 
     4201 GCGAGCTCTC TGCACGACTT CACCTCTGGG CATTCAAAAT GGATTATGAA ACTACAGAAA 
     4261 AGGAAGTAGC AGAACCACTC CTGGACCTGA AGGAAGGAAT AGACCAGTTG GAGAACAATA 
     4321 AAACCTTGGG CTTTATCCTG TCTACTCTCT TAGCCATTGG GAACTTTCTA AATGGAACTA 
     4381 ATGCCAAAGC GTTTGAGTTA AGCTACCTCG AGAAGGTTCC AGAAGTCAAA GACACAGTGC 
     4441 ACAAGCAGTC GCTTCTCCAC CATGTGTGCA CCATGGTGGT AGAAAACTTC CCAGACAGCT 
     4501 CCGATCTGTA CTCGGAGATC GGGGCCATCA CCAGGTCAGC CAAGGTTGAC TTTGATCAAC 
     4561 TTCAGGATAA TTTATGTCAG ATGGAGAGAA GATGCAAAGC TTCATGGGAT CACCTCAAGG 
     4621 CAATTGCAAA ACATGAAATG AAACCAGTTT TAAAACAACG GATGTCAGAG TTCCTGAAAG 
     4681 ACTGTGCAGA GCGAATTATA ATTTTAAAGA TTGTCCATAG AAGGATAATC AACAGATTCC 
     4741 ACTCCTTTTT ACTCTTTATG GGCCATCCAC CTTATGCAAT TCGGGAAGTG AACATAAACA 
     4801 AATTCTGCAG GATTATTAGT GAATTTGCAC TAGAGTATCG CACAACCAGG GAAAGGGTTT 
     4861 TGCAGCAGAA ACAGAAACGG GCCAACCACA GAGAGAGAAA TAAGACCAGA GGGAAGATGA 
     4921 TCACCGATTC TGGCAAGTTC TCCGGCAGTT CTCCGGCGCC CCCAAGCCAG CCGCAGGGTC 
     4981 TGAGCTATGC GGAGGACGCG GCTGAGCACG AGAACATGAA GGCTGTGCTG AAAACCTCGT 
     5041 CCCCCTCCGT GGAGGACGCC ACCCCCGCGC TGGGCGTCCG CACACGCAGC CGAGCAAGCC 
     5101 GAGGATCCAC TAGTTCCTGG ACTATGGGAA CTGATGACTC GCCCAATGTC ACAGATGATG 
     5161 CAGCTGATGA GATCATGGAC CGCATCGTCA AGTCAGCCAC CCAAGTGCCC AGTCAGCGAG 
     5221 TGGTGCCGAG GGAGAGGAAA CGATCCCGGG CCAACCGGAA ATCTTTGCGA AGAACCCTGA 
     5281 AGAGCGGCCT GACCCCAGAA GAAGCCAGAG CCCTGGGCTT GGTTGGCACC TCGGAGTTGC 
     5341 AGCTGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     5401 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     5461 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     5521 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5581 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5641 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5701 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5761 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5821 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5881 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5941 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     6001 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     6061 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     6121 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     6181 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     6241 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     6301 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     6361 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     6421 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     6481 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6541 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6601 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6661 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6721 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6781 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6841 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6901 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6961 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     7021 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     7081 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     7141 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     7201 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     7261 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     7321 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     7381 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     7441 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     7501 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7561 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7621 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7681 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7741 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7801 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7861 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7921 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7981 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     8041 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     8101 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     8161 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     8221 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     8281 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     8341 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     8401 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     8461 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8521 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8581 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8641 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8701 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8761 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8821 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8881 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8941 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     9001 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     9061 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     9121 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     9181 AAATTTAACG CGAATTTT
//