LOCUS       RC221138                8763 bp    DNA     circular  SYN 
DEFINITION  ASTN1 (Myc-DDK-tagged)-Human astrotactin 1 (ASTN1), transcript variant 1
ACCESSION   NM_004319
VERSION     NM_004319.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4920..5011 
                     /label="Myc-DDK"  
     misc_feature    5041..5626 
                     /label="PolyA signal"  
     misc_feature    5098..5117 
                     /label="Restriction Enzyme"  
     misc_feature    5902..6574 
                     /label="ColE1"  
     misc_feature    7004..7798 
                     /label="Kan/Neo"  
     misc_feature    7833..8135 
                     /label="SV40 ori"  
     misc_feature    8221..8698 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4902..4908 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4902 
                     /label="ASTN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTTAGCC GGGCTCTGCG CCCTGCTCGC CTGCTGCTGG GGGCCGGCGG 
     1081 CGGTGCTGGC CACGGCCGCC GGCGACGTGG ATCCATCCAA GGAGCTGGAG TGCAAGCTCA 
     1141 AAAGCATCAC GGTGTCGGCA CTGCCCTTCC TGCGCGAGAA CGACCTGAGC ATCATGCACA 
     1201 GCCCCTCGGC CTCGGAGCCC AAGCTCCTCT TCTCGGTGCG CAACGACTTC CCGGGAGAAA 
     1261 TGGTCGTGGT GGACGACCTG GAGAACACGG AGCTGCCCTA CTTCGTGCTG GAGATCTCAG 
     1321 GGAACACAGA GGATATCCCT TTGGTGCGCT GGAGGCAGCA GTGGCTGGAG AATGGCACTT 
     1381 TGCTTTTTCA CATTCATCAC CAAGATGGTG CCCCAAGCCT TCCTGGACAA GACCCCACTG 
     1441 AAGAACCCCA ACATGAGTCG GCAGAAGAGG AGCTGAGGAT CCTCCACATC TCAGTCATGG 
     1501 GTGGCATGAT CGCTCTGCTG CTGTCCATCT TGTGCCTGGT GATGATCCTG TATACTCGCC 
     1561 GGCGCTGGTG CAAACGCCGC CGGGTCCCGC AGCCCCAGAA GAGTGCCAGT GCTGAGGCAG 
     1621 CCAATGAGAT TCACTACATT CCTTCTGTGC TGATCGGCGG GCACGGACGG GAGAGCCTGC 
     1681 GCAATGCCCG CGTGCAGGGC CACAACTCCA GTGGCACCCT GAGCATCCGG GAGACACCTA 
     1741 TCCTGGACGG CTATGAGTAT GACATCACTG ATCTGCGCCA CCATCTGCAG AGGGAGTGCA 
     1801 TGAACGGAGG GGAGGACTTT GCCAGCCAGG TCACGCGCAC CCTCGACTCC CTGCAGGGCT 
     1861 GCAATGAAAA GTCGGGGATG GACCTCACAC CAGGAAGTGA CAATGCCAAG CTGTCACTGA 
     1921 TGAACAAGTA TAAAGATAAT ATTATAGCCA CTAGCCCTGT GGACTCCAAC CACCAGCAAG 
     1981 CCACCCTTCT CTCTCACACC TCCAGCAGCC AGAGAAAGCG GATCAACAAC AAAGCAAGAG 
     2041 CTGGTTCCGC CTTCTTGAAC CCTGAAGGGG ATTCTGGCAC AGAGGCAGAA AACGACCCCC 
     2101 AGCTGACCTT TTACACGGAT CCTTCAAGGA GCAGGAGGCG TAGTAGAGTG GGTTCTCCCC 
     2161 GAAGTCCTGT GAATAAGACC ACCTTGACCC TGATCAGCAT CACCAGCTGT GTGATTGGCC 
     2221 TCGTGTGCTC CTCTCACGTC AACTGCCCTC TCGTTGTCAA GATCACCCTG CATGTCCCTG 
     2281 AGCACCTGAT TGCTGATGGG AGCCGCTTCA TCTTGCTGGA GGGGAGCCAG CTGGATGCCA 
     2341 GTGACTGGCT GAACCCTGCC CAAGTGGTTC TCTTCTCTCA GCAGAACTCC AGCGGACCCT 
     2401 GGGCCATGGA CCTCTGTGCC CGGCGGCTCC TGGACCCCTG TGAACACCAA TGTGACCCCG 
     2461 AAACTGGGGA ATGCCTCTGC TATGAAGGCT ACATGAAGGA TCCAGTACAT AAGCACCTTT 
     2521 GCATTCGGAA CGAATGGGGG ACAAACCAGG GGCCATGGCC TTACACAATA TTTCAGCGAG 
     2581 GCTTTGACCT GGTTTTGGGA GAGCAGCCCT CTGATAAAAT ATTTAGATTC ACCTACACTC 
     2641 TTGGGGAGGG CATGTGGTTG CCCCTCAGCA AGAGCTTTGT GATTCCACCA GCCGAACTGG 
     2701 CCATCAATCC ATCAGCAAAG TGCAAGACGG ACATGACTGT GATGGAGGAT GCTGTGGAGG 
     2761 TCAGAGAGGA GCTGATGACT TCATCCTCCT TCGACAGCCT GGAGGTTCTC TTAGATTCCT 
     2821 TTGGGCCGGT GCGCGACTGC AGCAAAGATA ACGGGGGCTG CAGTAAGAAT TTCCGCTGTA 
     2881 TTTCAGATCG CAAGCTGGAC TCCACTGGTT GCGTGTGCCC ATCTGGACTC AGTCCCATGA 
     2941 AGGACAGCTC TGGCTGCTAT GACCGCCACA TCGGGGTGGA CTGTTCCGAC GGCTTCAACG 
     3001 GCGGCTGTGA GCAGCTGTGC CTCCAGCAGA TGGCGCCCTT CCCGGACGAC CCCACCTTGT 
     3061 ATAACATCCT CATGTTCTGT GGGTGCATCG AGGACTACAA GCTTGGTGTG GATGGACGCT 
     3121 CTTGCCAACT CATCACGGAG ACCTGTCCAG AGGGAAGTGA CTGTGGGGAA AGCAGGGAGC 
     3181 TTCCCATGAA CCAGACCCTC TTTGGGGAGA TGTTCTTTGG TTACAACAAC CATTCCAAGG 
     3241 AAGTGGCTGC CGGACAGGTG CTGAAAGGAA CATTCAGGCA AAACAACTTT GCTCGTGGTT 
     3301 TAGACCAGCA ACTGCCAGAT GGTCTTGTGG TGGCCACTGT GCCCCTGGAG AATCAATGCC 
     3361 TAGAGGAGAT CTCGGAGCCC ACCCCTGACC CTGACTTCCT GACTGGGATG GTGAACTTCA 
     3421 GTGAAGTGTC TGGGTACCCT GTGCTGCAGC ACTGGAAGGT CCGGTCTGTG ATGTACCACA 
     3481 TCAAACTCAA CCAAGTGGCC ATCTCTCAGG CCCTCAGCAA TGCTCTCCAC TCGCTGGATG 
     3541 GGGCTACATC TCGTGCAGAT TTTGTGGCGC TGTTGGACCA GTTCGGCAAC CATTACATCC 
     3601 AGGAAGCTAT CTACGGCTTT GAGGAGTCCT GTTCTATCTG GTACCCAAAC AAGCAGGTCC 
     3661 AGCGGCGACT CTGGCTGGAG TATGAAGACA TCAGTAAAGG CAACTCCCCA TCAGATGAGT 
     3721 CTGAGGAGCG GGAAAGAGAC CCCAAGGTGC TGACATTCCC AGAATACATC ACCAGCTTGT 
     3781 CAGACTCCGG CACCAAGCAC ATGGCGGCTG GAGTCCGCAT GGAGTGCCAC AGCAAGGGAC 
     3841 GATGCCCCTC GTCCTGCCCC CTGTGTCATG TGACATCCAG CCCTGACACC CCTGCTGAGC 
     3901 CGGTTCTGCT GGAGGTGACC AAAGCAGCCC CCATCTATGA ACTAGTGACC AACAACCAGA 
     3961 CCCAGAGGCT CTTGCAGGAG GCTACCATGA GCTCTCTCTG GTGCTCAGGG ACTGGAGATG 
     4021 TCATCGAGGA CTGGTGTCGA TGTGACTCCA CTGCTTTTGG AGCTGATGGA CTCCCCACCT 
     4081 GTGCGCCTCT CCCACAGCCT GTGCTGAGAC TCTCCACGGT TCACGAGCCC AGCAGCACTC 
     4141 TTGTGGTCCT GGAGTGGGAA CACTCAGAGC CACCAATCGG GGTGCAGATT GTAGATTACC 
     4201 TCCTCCGTCA AGAGAAAGTC ACTGACAGGA TGGACCACTC CAAAGTGGAG ACAGAAACAG 
     4261 TGCTGAGCTT TGTGGACGAC ATCATCTCTG GAGCAAAGTC TCCTTGTGCA ATGCCATCTC 
     4321 AGGTGCCGGA CAAGCAGCTC ACCACCATCT CTCTGATCAT ACGATGCCTG GAACCTGACA 
     4381 CCATTTACAT GTTCACGCTG TGGGGAGTGG ACAACACAGG ACGGCGCTCC AGGCCAAGCG 
     4441 ACGTGATCGT GAAGACCCCA TGCCCCGTGG TGGATGATGT CAAGGCTCAA GAAATAGCAG 
     4501 ACAAGATCTA CAATCTCTTC AATGGCTACA CTAGTGGGAA GGAGCAGCAG ACCGCCTACA 
     4561 ACACCCTCCT GGATCTGGGT TCCCCCACCT TACACCGGGT CCTCTACCAC TATAACCAGC 
     4621 ACTATGAGAG TTTTGGGGAA TTCACCTGGC GATGTGAGGA TGAGTTAGGT CCCAGGAAAG 
     4681 CTGGTCTCAT CCTTTCCCAG CTTGGGGACC TCAGCAGTTG GTGCAATGGA CTCCTTCAGG 
     4741 AACCCAAGAT AAGCTTGCGG CGCAGCTCAC TCAAGTACCT GGGGTGCCGC TACAGCGAGA 
     4801 TCAAACCCTA CGGACTTGAC TGGGCGGAGC TCAGCCGGGA CCTCAGGAAG ACGTGTGAGG 
     4861 AGCAGACCCT GAGTATCCCC TACAACGACT ATGGGGACAG CAAAGAGATC ACGCGTACGC 
     4921 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4981 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5041 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5101 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5161 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5221 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5281 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5341 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5401 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5461 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5521 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5581 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5641 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5701 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5761 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5821 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5881 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5941 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6001 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6061 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6121 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6181 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6241 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6301 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6361 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6421 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6481 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6541 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6601 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6661 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6721 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6781 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6841 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6901 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6961 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7021 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7081 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7141 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7201 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7261 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7321 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7381 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7441 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7501 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7561 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7621 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7681 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7741 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7801 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7861 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7921 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7981 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8041 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8101 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8161 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8221 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8281 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8341 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8401 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8461 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8521 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8581 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8641 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8701 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8761 TTT
//