LOCUS       RC221118                8181 bp    DNA     circular  SYN 
DEFINITION  SORBS2 (Myc-DDK-tagged)-Human sorbin and SH3 domain containing 2 (SORBS2), transcript variant 2
ACCESSION   NM_021069
VERSION     NM_021069.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4338..4429 
                     /label="Myc-DDK"  
     misc_feature    4459..5044 
                     /label="PolyA signal"  
     misc_feature    4516..4535 
                     /label="Restriction Enzyme"  
     misc_feature    5320..5992 
                     /label="ColE1"  
     misc_feature    6422..7216 
                     /label="Kan/Neo"  
     misc_feature    7251..7553 
                     /label="SV40 ori"  
     misc_feature    7639..8116 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4320..4326 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4320 
                     /label="SORBS2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTACTAT CAGAGGCCGT TTTCCCCCTC GGCATATTCT CTCCCAGCCT 
     1081 CACTCAACTC CAGCATTGTC ATGCAGCACG GCACATCCCT CGATTCCACA GACACATATC 
     1141 CCCAGCATGC GCAGTCTCTG GATGGCACCA CCAGCAGCTC TATCCCCCTG TACCGATCCT 
     1201 CAGAGGAAGA GAAGAGAGTG ACAGTCATCA AAGCCCCGCA TTACCCAGGG ATCGGGCCCG 
     1261 TGGATGAATC CGGAATCCCC ACAGCAATTA GAACGACAGT CGACCGGCCC AAGGACTGGT 
     1321 ACAAGACGAT GTTTAAGCAA ATTCACATGG TGCACAAGCC GGATGATGAC ACAGACATGT 
     1381 ATAATACTCC TTATACATAC AATGCAGGTC TGTACAACCC ACCCTACAGT GCTCAGTCAC 
     1441 ACCCTGCTGC AAAGACCCAA ACCTACAGAC CTCTTTCCAA AAGCCACTCC GACAACAGCC 
     1501 CCAATGCCTT TAAGGATGCG TCCTCCCCAG TGCCTCCCCC ACATGTTCCA CCTCCAGTCC 
     1561 CGCCGCTTCG ACCAAGAGAT CGGTCTTCAA CAGAAAAGCA TGACTGGGAT CCTCCAGACA 
     1621 GAAAAGTGGA CACAAGAAAA TTTCGGTCTG AGCCAAGGAG TATTTTTGAA TATGAACCTG 
     1681 GCAAGTCATC AATTCTTCAG CATGAAAGAC CAGCCTCCTT GTATCAGTCC TCTATAGACA 
     1741 GAAGCCTGGA AAGACCCATG AGTTCTGCAA GCATGGCCAG TGACTTCAGG AAGCGGAGGA 
     1801 AGAGCGAGCC TGCAGTGGGT CCACCACGGG GCTTGGGAGA TCAAAGTGCG AGCAGGACTA 
     1861 GCCCAGGCCG AGTGGACCTC CCAGGATCAA GCACCACTCT TACAAAGTCT TTCACTAGCT 
     1921 CTTCTCCTTC TTCCCCATCA AGAGCAAAAG GTGGGGATGA TAGCAAAATA TGTCCATCCC 
     1981 TTTGCAGTTA CTCAGGGCTC AACGGCAACC CCTCCAGTGA GTTAGATTAC TGTAGCACTT 
     2041 ACAGACAGCA CTTGGATGTC CCTCGGGACT CACCAAGAGC CATTAGTTTC AAGAACGGCT 
     2101 GGCAAATGGC CCGGCAAAAC GCAGAAATCT GGAGCAGCAC GGAAGAAACC GTCTCTCCCA 
     2161 AAATCAAATC CCGGAGCTGT GACGATCTCC TAAACGACGA CTGCGATAGC TTCCCGGACC 
     2221 CGAAAGTCAA GTCGGAAAGC ATGGGCTCCC TGTTATGTGA GGAGGACTCC AAGGAGAGCT 
     2281 GCCCCATGGC GTGGGGGTCC CCCTACGTCC CGGAGGTTCG CAGTAACGGC AGATCAAGGA 
     2341 TCAGACACAG GTCAGCCCGC AACGCCCCGG GGTTCCTGAA GATGTACAAG AAAATGCACC 
     2401 GCATCAACCG CAAGGACCTG ATGAACTCCG AGGTGATCTG CTCCGTGAAG TCCAGGATTC 
     2461 TGCAGTACGA GAGCGAGCAG CAGCACAAGG ACCTGCTGCG CGCCTGGAGC CAGTGCTCCA 
     2521 CGGAGGAGGT GCCCCGGGAC ATGGTGCCCA CACGCATCTC CGAATTCGAA AAGCTGATCC 
     2581 AGAAGTCCAA ATCCATGCCA AATTTAGGGG ATGACATGCT GTCTCCCGTA ACCCTAGAAC 
     2641 CACCGCAAAA TGGCCTATGT CCCAAGAGGA GGTTTTCCAT TGAGTATTTG CTGGAGGAAG 
     2701 AAAATCAAAG CGGCCCCCCC GCTCGGGGCC GGCGAGGCTG CCAGTCTAAC GCCCTGGTGC 
     2761 CCATTCACAT TGAAGTCACC AGCGATGAGC AGCCCCGAGC ACACGTGGAG TTTTCCGACA 
     2821 GCGACCAGGA CGGGGTTGTG TCCGACCACA GTGACTACAT TCACCTAGAG GGGTCATCCT 
     2881 TCTGCAGTGA AAGTGACTTT GATCACTTTT CCTTCACATC CTCCGAAAGC TTTTACGGAT 
     2941 CCAGCCACCA CCACCACCAT CACCACCACC ACCACCACCG CCACCTCATC AGCTCCTGCA 
     3001 AAGGCAGGTG CCCGGCCTCC TACACTCGAT TTACCACAAT GTTAAAACAC GAAAGAGCCA 
     3061 GACACGAAAA CACCGAGGAG CCCAGAAGGC AAGAAATGGA CCCTGGCCTT TCTAAACTTG 
     3121 CTTTTCTAGT CAGTCCTGTG CCTTTCCGGA GGAAAAAAAA TTCGGCTCCT AAGAAACAGA 
     3181 CTGAAAAGGC AAAATGTAAA GCATCTGTGT TTGAGGCTCT GGACTCTGCC CTTAAAGACA 
     3241 TCTGTGACCA AATTAAAGCT GAAAAGAAAA GGGGGAGCTT GCCGGACAAC AGCATCCTGC 
     3301 ACCGCCTCAT CAGTGAGCTG CTGCCAGATG TTCCCGAGAG GAACTCATCC CTGAGAGCGC 
     3361 TGAGGAGGAG CCCCCTGCAC CAGCCTCTCC ACCCACTGCC TCCCGATGGT GCTATTCATT 
     3421 GTCCACCCTA CCAGAATGAC TGCGGGAGAA TGCCCCGCAG TGCCTCTTTC CAAGACGTGG 
     3481 ACACAGCCAA CAGCAGCTGC CACCACCAAG ACCGTGGCGG TGCACTCCAA GACCGTGAGT 
     3541 CCCCTAGAAG TTACTCATCC ACTTTGACTG ACATGGGGAG AAGTGCACCA AGGGAAAGAA 
     3601 GAGGAACTCC AGAAAAAGAG AAATTGCCTG CAAAAGCTGT TTATGATTTT AAGGCTCAGA 
     3661 CATCTAAGGA GTTGTCATTT AAGAAAGGAG ATACTGTCTA CATCCTCAGG AAAATTGATC 
     3721 AAAATTGGTA TGAGGGAGAA CACCACGGGA GAGTGGGCAT CTTCCCGATC TCATACGTAG 
     3781 AGAAACTCAC ACCTCCTGAG AAAGCACAGC CTGCAAGACC ACCTCCGCCA GCCCAGCCCG 
     3841 GAGAAATCGG AGAAGCTATA GCCAAATACA ACTTCAACGC AGACACAAAT GTGGAGCTGT 
     3901 CACTGAGAAA GGGAGATAGA GTTATTCTTC TTAAAAGAGT TGATCAAAAC TGGTATGAAG 
     3961 GTAAAATCCC AGGAACCAAC AGACAAGGCA TCTTCCCTGT TTCCTATGTG GAGGTCGTCA 
     4021 AGAAGAACAC AAAAGGTGCT GAGGACTACC CTGACCCTCC AATACCCCAC AGCTATTCTA 
     4081 GTGATAGGAT TCACAGCTTG AGCTCAAATA AGCCACAGCG TCCTGTGTTT ACTCATGAAA 
     4141 ATATTCAAGG TGGGGGGGAA CCGTTTCAGG CTCTGTATAA CTATACTCCC AGGAATGAAG 
     4201 ATGAGCTGGA GCTCAGAGAA AGTGATGTCA TTGATGTCAT GGAAAAGTGT GATGACGGCT 
     4261 GGTTTGTGGG GACCTCAAGA AGAACCAAAT TCTTTGGTAC TTTCCCCGGA AACTACGTCA 
     4321 AGAGGCTGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4381 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4441 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4501 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4561 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4621 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4681 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4741 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4801 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4861 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4921 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4981 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5041 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5101 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5161 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5221 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5281 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5341 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5401 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5461 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5521 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5581 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5641 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5701 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5761 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5821 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5881 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5941 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6001 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6061 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6121 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6181 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6241 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6301 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6361 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6421 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6481 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6541 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6601 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6661 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6721 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6781 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6841 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6901 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6961 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7021 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7081 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7141 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7201 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7261 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7321 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7381 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7441 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7501 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7561 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7621 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7681 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7741 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7801 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7861 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7921 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7981 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8041 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8101 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8161 CAAAAATTTA ACGCGAATTT T
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