LOCUS       RC221101                8586 bp    DNA     circular  SYN 
DEFINITION  SMC1B (Myc-DDK-tagged)-Human structural maintenance of chromosomes 1B (SMC1B)
ACCESSION   NM_148674
VERSION     NM_148674.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4743..4834 
                     /label="Myc-DDK"  
     misc_feature    4864..5449 
                     /label="PolyA signal"  
     misc_feature    4921..4940 
                     /label="Restriction Enzyme"  
     misc_feature    5725..6397 
                     /label="ColE1"  
     misc_feature    6827..7621 
                     /label="Kan/Neo"  
     misc_feature    7656..7958 
                     /label="SV40 ori"  
     misc_feature    8044..8521 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4725..4731 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4725 
                     /label="SMC1B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCACCTG GAGCTGCTGC TTGTGGAAAA TTTCAAGTCG TGGCGGGGCC 
     1081 GCCAGGTCAT TGGCCCCTTC CGGAGGTTCA CCTGCATCAT CGGCCCCAAC GGCTCTGGAA 
     1141 AATCTAATGT AATGGATGCA CTTAGTTTTG TAATGGGAGA GAAAATAGCT AATTTAAGAG 
     1201 TGAAAAATAT TCAAGAACTC ATTCATGGAG CACATATTGG AAAACCTATT TCTTCTTCTG 
     1261 CAAGTGTAAA AATTATATAT GTGGAGGAAA GTGGCGAAGA GAAAACATTT GCAAGGATTA 
     1321 TCCGAGGGGG ATGCTCAGAA TTTCGCTTTA ATGATAATCT TGTGAGTCGT TCTGTTTACA 
     1381 TTGCAGAGTT GGAAAAGATA GGCATAATAG TCAAAGCACA AAATTGTTTG GTTTTTCAGG 
     1441 GAACTGTAGA GTCAATTTCA GTGAAGAAAC CCAAAGAAAG GACCCAGTTT TTTGAGGAAA 
     1501 TCAGCACTTC AGGAGAGCTT ATAGGAGAAT ATGAAGAAAA GAAAAGAAAG TTACAAAAAG 
     1561 CCGAAGAGGA TGCACAGTTT AACTTTAATA AGAAAAAAAA TATAGCGGCA GAGCGCAGAC 
     1621 AAGCAAAATT AGAGAAGGAA GAGGCAGAAC GTTACCAGAG TCTCCTTGAA GAACTGAAAA 
     1681 TGAACAAGAT ACAACTGCAG CTTTTTCAAC TATACCATAA TGAGAAAAAG ATTCATCTCC 
     1741 TGAACACCAA GTTAGAGCAT GTGAATAGGG ATTTGAGTGT CAAAAGAGAG TCTTTGTCTC 
     1801 ATCATGAAAA CATAGTTAAA GCCAGGAAAA AGGAACATGG AATGCTAACT AGACAACTAC 
     1861 AACAAACAGA AAAAGAATTA AAATCGGTTG AAACCCTTTT AAATCAGAAG AGGCCTCAGT 
     1921 ACATTAAAGC CAAAGAAAAC ACTTCTCACC ACCTTAAGAA ATTAGATGTG GCTAAGAAAT 
     1981 CAATAAAGGA CAGCGAAAAA CAATGTTCTA AACAGGAAGA TGATATAAAA GCCCTGGAGA 
     2041 CAGAGCTGGC TGATTTAGAT GCTGCATGGA GAAGTTTTGA AAAGCAGATT GAGGAAGAAA 
     2101 TTTTACATAA AAAGCGAGAC ATTGAACTGG AAGCCAGTCA GCTGGATCGT TATAAAGAAC 
     2161 TTAAGGAACA AGTAAGAAAG AAAGTAGCTA CAATGACTCA ACAACTGGAA AAACTGCAGT 
     2221 GGGAACAGAA GACAGATGAA GAAAGACTGG CATTTGAAAA GAGGAGGCAT GGAGAAGTTC 
     2281 AGGGAAATCT AAAACAAATA AAAGAACAAA TAGAAGATCA TAAAAAACGA ATAGAGAAGT 
     2341 TAGAGGAGTA TACAAAGACA TGCATGGATT GCTTGAAAGA GAAAAAACAG CAAGAGGAAA 
     2401 CCCTAGTGGA TGAAATTGAA AAAACAAAAT CAAGAATGTC TGAATTTAAT GAAGAATTGA 
     2461 ATCTTATTAG AAGTGAATTG CAGAATGCTG GGATTGATAC CCATGAGGGA AAACGTCAGC 
     2521 AAAAGAGAGC AGAGGTTCTG GAACACCTTA AAAGACTGTA CCCAGATTCT GTGTTTGGAA 
     2581 GACTATTTGA CCTGTGTCAT CCTATTCATA AGAAATACCA GCTGGCTGTT ACTAAGGTTT 
     2641 TTGGCCGGTT CATCACTGCC ATTGTTGTAG CCTCTGAAAA GGTAGCAAAA GATTGTATTC 
     2701 GATTTCTGAA GGAGGAAAGA GCTGAACCTG AGACATTCCT CGCTCTAGAT TACCTTGATA 
     2761 TCAAGCCAAT CAATGAAAGA CTAAGGGAGC TTAAAGGCTG TAAAATGGTG ATTGATGTCA 
     2821 TAAAGACTCA GTTTCCTCAG CTGAAGAAAG TGATTCAGTT TGTGTGTGGA AATGGTCTTG 
     2881 TTTGTGAGAC TATGGAAGAA GCAAGGCATA TTGCACTCAG TGGACCTGAA AGACAGAAAA 
     2941 CAGTAGCTCT TGATGGAACA TTATTTTTAA AATCTGGAGT GATCTCTGGA GGGTCAAGTG 
     3001 ACTTAAAATA CAAGGCTAGA TGCTGGGATG AGAAAGAGTT AAAGAATCTA AGAGACAGAC 
     3061 GAAGCCAGAA AATCCAAGAG CTAAAGGGTT TAATGAAGAC ACTCCGCAAA GAAACAGATT 
     3121 TGAAACAAAT ACAGACCCTG ATACAGGGAA CTCAAACACG ACTCAAATAT TCACAAAATG 
     3181 AACTAGAGAT GATTAAGAAG AAGCACCTTG TTGCTTTTTA CCAGGAACAA TCTCAGTTAC 
     3241 AAAGTGAACT ACTAAATATT GAGTCTCAAT GTATTATGTT GAGTGAAGGA ATCAAGGAAC 
     3301 GACAACGAAG AATTAAAGAA TTTCAAGAAA AGATAGATAA GGTAGAAGAC GATATCTTCC 
     3361 AACACTTCTG TGAAGAAATT GGCGTGGAAA ATATTCGTGA ATTTGAGAAC AAACATGTTA 
     3421 AACGGCAACA AGAAATTGAT CAAAAAAGAT TAGAATTTGA AAAACAAAAA ACTCGGCTTA 
     3481 ATGTTCAACT TGAGTATAGT CGCAGTCACC TTAAGAAGAA ACTGAATAAG ATCAACACAT 
     3541 TAAAAGAAAC TATCCAGAAA GGTAGTGAAG ATATTGATCA CCTAAAGAAG GCTGAAGAAA 
     3601 ACTGTCTGCA GACAGTGAAT GAACTCATGG CAAAGCAGCA GCAACTTAAG GACATACGTG 
     3661 TCACTCAGAA CTCCAGTGCC GAGAAAGTTC AAACTCAAAT TGAAGAGGAA CGGAAGAAGT 
     3721 TTCTGGCTGT TGATAGGGAA GTGGGGAAAT TGCAAAAAGA AGTTGTAAGT ATTCAAACTT 
     3781 CTCTGGAACA GAAACGATTA GAGAAGCATA ACTTGCTGCT TGATTGCAAA GTGCAAGACA 
     3841 TTGAGATAAT CCTTTTGTCG GGGTCACTGG ATGACATCAT TGAAGTGGAG ATGGGAACTG 
     3901 AAGCAGAAAG TACCCAGGCA ACAATTGATA TCTATGAAAA AGAAGAAGCC TTTGAAATAG 
     3961 ACTACAGCTC TCTAAAAGAG GATTTGAAGG CTCTACAGTC TGATCAAGAA ATCGAGGCCC 
     4021 ACCTTAGGCT CTTATTGCAG CAAGTAGCAT CCCAGGAAGA TATCTTACTG AAAACAGCAG 
     4081 CCCCAAACCT ACGAGCACTG GAGAACTTAA AGACTGTCAG AGACAAGTTT CAAGAGTCCA 
     4141 CAGATGCTTT TGAGGCCAGC AGAAAGGAAG CCAGACTGTG TAGGCAAGAG TTCGAGCAAG 
     4201 TGAAAAAAAG GAGATACGAT CTTTTCACCC AGTGTTTTGA GCATGTCTCA ATCTCAATTG 
     4261 ATCAAATCTA CAAGAAGCTC TGCAGAAACA ACAGCGCCCA AGCATTTCTT AGCCCAGAGA 
     4321 ACCCTGAAGA ACCTTACTTG GAGGGAATTA GCTATAACTG TGTGGCCCCA GGCAAACGGT 
     4381 TTATGCCAAT GGACAATTTG TCAGGGGGAG AAAAGTGTGT GGCAGCCTTG GCTCTCCTGT 
     4441 TTGCTGTGCA CAGTTTTCGT CCTGCCCCAT TCTTTGTTTT AGATGAAGTG GATGCAGCCC 
     4501 TAGACAATAC TAACATAGGC AAAGTGTCAA GTTACATCAA AGAGCAAACT CAAGACCAGT 
     4561 TTCAGATGAT AGTCATCTCC CTAAAAGAAG AGTTCTATTC CAGAGCCGAC GCGCTGATCG 
     4621 GCATCTATCC TGAGTACGAT GACTGCATGT TCAGCCGAGT TTTGACCCTA GATCTTTCTC 
     4681 AGTATCCAGA CACTGAAGGC CAAGAAAGCA GCAAGAGACA CGGAGAGTCC CGCACGCGTA 
     4741 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4801 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4861 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4921 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4981 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5041 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5101 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5161 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5221 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5281 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5341 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5401 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5461 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5521 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5581 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5641 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5701 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5761 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5821 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5881 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5941 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6001 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6061 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6121 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6181 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6241 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6301 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6361 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6421 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6481 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6541 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6601 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6661 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6721 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6781 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6841 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6901 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6961 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7021 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7081 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7141 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7201 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7261 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7321 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7381 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7441 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7501 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7561 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7621 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7681 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7741 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7801 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7861 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7921 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7981 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8041 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8101 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8161 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8221 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8281 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8341 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8401 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8461 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8521 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8581 AATTTT
//