LOCUS       RC221094                8328 bp    DNA     circular  SYN 
DEFINITION  KCNU1 (Myc-DDK-tagged)-Human potassium channel, subfamily U, member 1 (KCNU1)
ACCESSION   NM_001031836
VERSION     NM_001031836.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4485..4576 
                     /label="Myc-DDK"  
     misc_feature    4606..5191 
                     /label="PolyA signal"  
     misc_feature    4663..4682 
                     /label="Restriction Enzyme"  
     misc_feature    5467..6139 
                     /label="ColE1"  
     misc_feature    6569..7363 
                     /label="Kan/Neo"  
     misc_feature    7398..7700 
                     /label="SV40 ori"  
     misc_feature    7786..8263 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4467..4473 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4467 
                     /label="KCNU1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTCAGACT AAGCTACGAA ATGAAACTTG GGAAGACTTG CCAAAAATGT 
     1081 CCTGCACAAC TGAGATCCAA GCAGCATTCA TTCTCTCTTC CTTTGTGACC TTCTTCAGTG 
     1141 GACTCATCAT CCTGTTGATC TTCAGGCTGA TCTGGAGATC TGTTAAAAAA TGGCAAATCA 
     1201 TCAAGGGAAC AGGAATTATC TTGGAACTGT TCACATCAGG TACCATCGCT AGGAGCCATG 
     1261 TAAGAAGCCT CCACTTCCAG GGACAATTTC GTGATCATAT AGAAATGTTG CTTTCAGCCC 
     1321 AGACCTTTGT GGGGCAAGTG TTGGTGATCC TTGTCTTTGT ACTAAGCATT GGGTCTCTTA 
     1381 TAATCTATTT CATCAATTCT GCTGACCCTG TTGGAAGCTG TTCATCATAT GAAGACAAAA 
     1441 CCATTCCTAT TGATTTGGTT TTCAATGCTT TCTTTAGTTT CTATTTTGGA TTGAGGTTTA 
     1501 TGGCAGCTGA TGACAAGATC AAGTTCTGGC TGGAGATGAA TTCAATCGTA GACATCTTTA 
     1561 CCATCCCACC AACCTTTATT TCTTATTATT TGAAGAGCAA TTGGCTAGGT TTAAGGTTCC 
     1621 TAAGAGCCTT GCGCCTGCTA GAACTCCCTC AAATCTTGCA AATTCTACGA GCCATCAAGA 
     1681 CCAGTAACTC AGTGAAGTTT TCCAAACTGC TGTCAATAAT TCTCAGTACC TGGTTCACAG 
     1741 CTGCGGGATT CATTCACCTG GTGGAAAATT CTGGTGATCC CTGGCTCAAA GGTAGAAATT 
     1801 CACAGAATAT ATCATATTTT GAGTCAATTT ACCTGGTCAT GGCAACAACG TCAACCGTTG 
     1861 GATTTGGAGA TGTGGTAGCC AAGACATCCT TAGGACGGAC CTTCATCATG TTCTTCACAC 
     1921 TGGGGAGTTT GATATTATTT GCGAACTATA TACCTGAAAT GGTGGAACTG TTTGCTAACA 
     1981 AGAGGAAATA CACCAGTTCC TATGAAGCAC TCAAAGGAAA GAAGTTTATT GTGGTCTGTG 
     2041 GAAACATCAC TGTGGACAGT GTGACCGCTT TCCTGAGGAA TTTCCTCCGC GACAAGTCAG 
     2101 GAGAGATCAA CACTGAAATT GTTTTCCTGG GAGAAACCCC TCCTTCTTTG GAACTTGAAA 
     2161 CCATATTTAA ATGCTACTTG GCCTACACAA CGTTCATTTC TGGATCTGCA ATGAAGTGGG 
     2221 AGGATCTGAG GCGAGTTGCG GTGGAATCTG CAGAGGCATG CCTGATTATA GCCAATCCTT 
     2281 TGTGCAGTGA TTCCCATGCT GAAGATATTT CCAACATTAT GAGGGTGCTC TCTATCAAGA 
     2341 ACTATGATTC TACCACCAGA ATCATCATAC AGATACTGCA ATCCCATAAC AAGGTTTATC 
     2401 TGCCAAAGAT TCCCAGCTGG AACTGGGACA CCGGAGACAA CATCATCTGC TTTGCTGAAT 
     2461 TAAAACTTGG ATTTATCGCC CAAGGCTGTT TGGTGCCAGG CTTGTGTACC TTCCTAACAT 
     2521 CTCTATTTGT GGAGCAAAAC AAAAAGGTTA TGCCTAAACA GACCTGGAAG AAACACTTCT 
     2581 TGAATAGCAT GAAAAACAAA ATTCTGACCC AACGTCTCTC TGATGACTTT GCTGGAATGA 
     2641 GCTTTCCTGA AGTTGCCCGG CTCTGCTTTC TGAAGATGCA CCTCCTGTTG ATAGCCATCG 
     2701 AATACAAGTC CCTCTTTACG GATGGTTTCT GTGGTCTGAT ACTAAATCCA CCTCCACAAG 
     2761 TGAGGATACG TAAGAACACA TTAGGGTTCT TTATTGCTGA AACTCCAAAG GACGTCAGAA 
     2821 GAGCCTTGTT TTACTGTTCA GTCTGTCATG ATGATGTGTT CATTCCTGAG CTAATTACAA 
     2881 ACTGTGGCTG CAAAAGCAGA AGCCGGCAGC ACATCACAGT GCCATCGGTA AAGAGAATGA 
     2941 AAAAATGTCT GAAGGGAATC TCCTCTCGTA TATCAGGGCA GGATTCTCCG CCAAGGGTAT 
     3001 CTGCAAGCAC TTCGAGCATA TCAAACTTCA CCACCAGGAC TCTTCAACAT GATGTAGAAC 
     3061 AAGATTCTGA CCAGCTTGAT AGCAGTGGGA TGTTTCACTG GTGCAAACCA ACCTCTTTGG 
     3121 ACAAGGTGAC TCTGAAACGA ACTGGCAAGT CAAAGTATAA GTTTCGGAAC CATATTGTAG 
     3181 CATGTGTATT TGGAGATGCC CACTCAGCCC CGATGGGGCT TCGGAACTTT GTAATGCCCT 
     3241 TGAGAGCCAG CAACTATACC AGGAAGGAGC TGAAGGACAT AGTGTTCATT GGGTCTCTGG 
     3301 ACTATCTACA GAGAGAATGG CGATTTCTCT GGAATTTTCC CCAGATATAC ATTCTGCCTG 
     3361 GATGTGCACT TTATTCTGGA GACCTCCATG CGGCCAACAT AGAGCAATGC TCCATGTGTG 
     3421 CTGTCTTGTC CCCCCCACCC CAGCCATCAA GCAACCAGAC TTTGGTAGAC ACAGAAGCCA 
     3481 TCATGGCAAC CCTCACCATC GGATCCTTGC AAATTGACTC CTCCTCTGAC CCGTCACCCT 
     3541 CAGTGTCAGA GGAGACTCCA GGTTACACAA ATGGACATAA TGAGAAATCA AACTGCCGAA 
     3601 AAGTCCCTAT CCTTACTGAA CTGAAAAATC CTTCCAACAT TCACTTTATT GAACAGCTTG 
     3661 GTGGACTGGA AGGGTCCCTC CAAGAAACAA ATCTGCATCT CAGCACTGCC TTTTCTACGG 
     3721 GCACTGTTTT TTCCGGCAGC TTCTTGGATT CTCTGCTGGC CACGGCCTTC TACAATTATC 
     3781 ATGTCCTGGA ATTGCTTCAG ATGCTGGTGA CAGGAGGAGT AAGTTCTCAG CTGGAACAAC 
     3841 ATTTAGATAA GGATAAAGTC TATGGTGTGG CAGATAGCTG CACGTCGCTC TTGTCTGGAA 
     3901 GAAACCGGTG TAAGCTGGGG CTTCTGTCCT TACACGAAAC CATTTTATCA GACGTTAATC 
     3961 CAAGAAACAC CTTTGGACAA CTGTTCTGTG GCTCATTAGA TCTTTTTGGA ATCCTGTGTG 
     4021 TTGGCTTATA CCGAATAATT GATGAAGAGG AGCTCAACCC AGAAAACAAA AGGTTTGTGA 
     4081 TCACCCGGCC AGCCAATGAG TTCAAGCTGC TGCCTTCAGA TCTTGTGTTT TGTGCCATAC 
     4141 CCTTCAGCAC TGCTTGTTAT AAAAGGAATG AAGAGTTCTC ATTGCAAAAG TCATATGAAA 
     4201 TTGTAAATAA AGCATCACAG ACAACAGAGA CACATTCAGA CACAAATTGT CCTCCCACCA 
     4261 TTGATTCAGT TACTGAGACA TTGTATTCAC CAGTCTATTC TTACCAGCCG AGAACTAACT 
     4321 CCCTCTCTTT TCCTAAGCAA ATAGCATGGA ATCAGAGTAG AACAAACAGT ATTATATCAT 
     4381 CTCAGATACC TTTAGGTGAC AATGCAAAAG AAAATGAAAG GAAAACTTCA GATGAGGTTT 
     4441 ATGATGAGGA TCCCTTTGCA TATTCAGAGC CACTAACGCG TACGCGGCCG CTCGAGCAGA 
     4501 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4561 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4621 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4681 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4741 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4801 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4861 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4921 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4981 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5041 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5101 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5161 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5221 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5281 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5341 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5401 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5461 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5521 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5581 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5641 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5701 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5761 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5821 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5881 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5941 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6001 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6061 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6121 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6181 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6241 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6301 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6361 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6421 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6481 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6541 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6601 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6661 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6721 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6781 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6841 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6901 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6961 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7021 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7081 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7141 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7201 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7261 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7321 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7381 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7441 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7501 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7561 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7621 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7681 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7741 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7801 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7861 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7921 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7981 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8041 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8101 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8161 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8221 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8281 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//