LOCUS       RC221039                8481 bp    DNA     circular  SYN 
DEFINITION  JAG2 (Myc-DDK-tagged)-Human jagged 2 (JAG2), transcript variant 2
ACCESSION   NM_145159
VERSION     NM_145159.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4638..4729 
                     /label="Myc-DDK"  
     misc_feature    4759..5344 
                     /label="PolyA signal"  
     misc_feature    4816..4835 
                     /label="Restriction Enzyme"  
     misc_feature    5620..6292 
                     /label="ColE1"  
     misc_feature    6722..7516 
                     /label="Kan/Neo"  
     misc_feature    7551..7853 
                     /label="SV40 ori"  
     misc_feature    7939..8416 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4620..4626 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4620 
                     /label="JAG2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGGCGCAG GGCCGGGGGC GCCTTCCCCG GCGGCTGCTG CTGCTGCTGG 
     1081 CGCTCTGGGT GCAGGCGGCG CGGCCCATGG GCTATTTCGA GCTGCAGCTG AGCGCGCTGC 
     1141 GGAACGTGAA CGGGGAGCTG CTGAGCGGCG CCTGCTGTGA CGGCGACGGC CGGACAACGC 
     1201 GCGCGGGGGG CTGCGGCCAC GACGAGTGCG ACACGTACGT GCGCGTGTGC CTTAAGGAGT 
     1261 ACCAGGCCAA GGTGACGCCC ACGGGGCCCT GCAGCTACGG CCACGGCGCC ACGCCCGTGC 
     1321 TGGGCGGCAA CTCCTTCTAC CTGCCGCCGG CGGGCGCTGC GGGGGACCGA GCGCGGGCGC 
     1381 GGGCCCGGGC CGGCGGCGAC CAGGACCCGG GCCTCGTCGT CATCCCCTTC CAGTTCGCCT 
     1441 GGCCGCGCTC CTTTACCCTC ATCGTGGAGG CCTGGGACTG GGACAACGAT ACCACCCCGA 
     1501 ATGAGGAGCT GCTGATCGAG CGAGTGTCGC ATGCCGGCAT GATCAACCCG GAGGACCGCT 
     1561 GGAAGAGCCT GCACTTCAGC GGCCACGTGG CGCACCTGGA GCTGCAGATC CGCGTGCGCT 
     1621 GCGACGAGAA CTACTACAGC GCCACTTGCA ACAAGTTCTG CCGGCCCCGC AACGACTTTT 
     1681 TCGGCCACTA CACCTGCGAC CAGTACGGCA ACAAGGCCTG CATGGACGGC TGGATGGGCA 
     1741 AGGAGTGCAA GGAAGCTGTG TGTAAACAAG GGTGTAATTT GCTCCACGGG GGATGCACCG 
     1801 TGCCTGGGGA GTGCAGGTGC AGCTACGGCT GGCAAGGGAG GTTCTGCGAT GAGTGTGTCC 
     1861 CCTACCCCGG CTGCGTGCAT GGCAGTTGTG TGGAGCCCTG GCAGTGCAAC TGTGAGACCA 
     1921 ACTGGGGCGG CCTGCTCTGT GACAAAGACC TGAACTACTG TGGCAGCCAC CACCCCTGCA 
     1981 CCAACGGAGG CACGTGCATC AACGCCGAGC CTGACCAGTA CCGCTGCACC TGCCCTGACG 
     2041 GCTACTCGGG CAGGAACTGT GAGAAGGCTG AGCACGCCTG CACCTCCAAC CCGTGTGCCA 
     2101 ACGGGGGCTC TTGCCATGAG GTGCCGTCCG GCTTCGAATG CCACTGCCCA TCGGGCTGGA 
     2161 GCGGGCCCAC CTGTGCCCTT GACATCGATG AGTGTGCTTC GAACCCGTGT GCGGCCGGTG 
     2221 GCACCTGTGT GGACCAGGTG GACGGCTTTG AGTGCATCTG CCCCGAGCAG TGGGTGGGGG 
     2281 CCACCTGCCA GCTGGACGTC AACGACTGTC GCGGGCAGTG TCAGCATGGG GGCACCTGCA 
     2341 AGGACCTGGT GAACGGGTAC CAGTGTGTGT GCCCACGGGG CTTCGGAGGC CGGCATTGCG 
     2401 AGCTGGAACG AGACGAGTGT GCCAGCAGCC CCTGCCACAG CGGCGGCCTC TGCGAGGACC 
     2461 TGGCCGACGG CTTCCACTGC CACTGCCCCC AGGGCTTCTC CGGGCCTCTC TGTGAGGTGG 
     2521 ATGTCGACCT TTGTGAGCCA AGCCCCTGCC GGAACGGCGC TCGCTGCTAT AACCTGGAGG 
     2581 GTGACTATTA CTGCGCCTGC CCTGATGACT TTGGTGGCAA GAACTGCTCC GTGCCCCGCG 
     2641 AGCCGTGCCC TGGCGGGGCC TGCAGAGTGA TCGATGGCTG CGGGTCAGAC GCGGGGCCTG 
     2701 GGATGCCTGG CACAGCAGCC TCCGGCGTGT GTGGCCCCCA TGGACGCTGC GTCAGCCAGC 
     2761 CAGGGGGCAA CTTTTCCTGC ATCTGTGACA GTGGCTTTAC TGGCACCTAC TGCCATGAGA 
     2821 ACATTGACGA CTGCCTGGGC CAGCCCTGCC GCAATGGGGG CACATGCATC GATGAGGTGG 
     2881 ACGCCTTCCG CTGCTTCTGC CCCAGCGGCT GGGAGGGCGA GCTCTGCGAC ACCAATCCCA 
     2941 ACGACTGCCT TCCCGATCCC TGCCACAGCC GCGGCCGCTG CTACGACCTG GTCAATGACT 
     3001 TCTACTGTGC GTGCGACGAC GGCTGGAAGG GCAAGACCTG CCACTCACGC GAGTTCCAGT 
     3061 GCGATGCCTA CACCTGCAGC AACGGTGGCA CCTGCTACGA CAGCGGCGAC ACCTTCCGCT 
     3121 GCGCCTGCCC CCCCGGCTGG AAGGGCAGCA CCTGCGCCGT CGCCAAGAAC AGCAGCTGCC 
     3181 TGCCCAACCC CTGTGTGAAT GGTGGCACCT GCGTGGGCAG CGGGGCCTCC TTCTCCTGCA 
     3241 TCTGCCGGGA CGGCTGGGAG GGTCGTACTT GCACTCACAA TACCAACGAC TGCAACCCTC 
     3301 TGCCTTGCTA CAATGGTGGC ATCTGTGTTG ACGGCGTCAA CTGGTTCCGC TGCGAGTGTG 
     3361 CACCTGGCTT CGCGGGGCCT GACTGCCGCA TCAACATCGA CGAGTGCCAG TCCTCGCCCT 
     3421 GTGCCTACGG GGCCACGTGT GTGGATGAGA TCAACGGGTA TCGCTGTAGC TGCCCACCCG 
     3481 GCCGAGCCGG CCCCCGGTGC CAGGAAGTGA TCGGGTTCGG GAGATCCTGC TGGTCCCGGG 
     3541 GCACTCCGTT CCCACACGGA AGCTCCTGGG TGGAAGACTG CAACAGCTGC CGCTGCCTGG 
     3601 ATGGCCGCCG TGACTGCAGC AAGGTGTGGT GCGGATGGAA GCCTTGTCTG CTGGCCGGCC 
     3661 AGCCCGAGGC CCTGAGCGCC CAGTGCCCAC TGGGGCAAAG GTGCCTGGAG AAGGCCCCAG 
     3721 GCCAGTGTCT GCGACCACCC TGTGAGGCCT GGGGGGAGTG CGGCGCAGAA GAGCCACCGA 
     3781 GCACCCCCTG CCTGCCACGC TCCGGCCACC TGGACAATAA CTGTGCCCGC CTCACCTTGC 
     3841 ATTTCAACCG TGACCACGTG CCCCAGGGCA CCACGGTGGG CGCCATTTGC TCCGGGATCC 
     3901 GCTCCCTGCC AGCCACAAGG GCTGTGGCAC GGGACCGCCT GCTGGTGTTG CTTTGCGACC 
     3961 GGGCGTCCTC GGGGGCCAGT GCTGTGGAGG TGGCCGTGTC CTTCAGCCCT GCCAGGGACC 
     4021 TGCCTGACAG CAGCCTGATC CAGGGCGCGG CCCACGCCAT CGTGGCCGCC ATCACCCAGC 
     4081 GGGGGAACAG CTCACTGCTC CTGGCTGTCA CCGAGGTCAA GGTGGAGACG GTTGTTACGG 
     4141 GCGGCTCTTC CACAGGTCTG CTGGTGCCTG TGCTGTGTGG TGCCTTCAGC GTGCTGTGGC 
     4201 TGGCGTGCGT GGTCCTGTGC GTGTGGTGGA CACGCAAGCG CAGGAAAGAG CGGGAGAGGA 
     4261 GCCGGCTGCC GCGGGAGGAG AGCGCCAACA ACCAGTGGGC CCCGCTCAAC CCCATCCGCA 
     4321 ACCCCATTGA GCGGCCGGGG GGCCACAAGG ACGTGCTCTA CCAGTGCAAG AACTTCACGC 
     4381 CGCCGCCGCG CAGGGCGGAC GAGGCGCTGC CCGGGCCGGC CGGCCACGCG GCCGTCAGGG 
     4441 AGGATGAGGA GGACGAGGAT CTGGGCCGCG GTGAGGAGGA CTCCCTGGAG GCGGAGAAGT 
     4501 TCCTCTCACA CAAATTCACC AAAGATCCTG GCCGCTCGCC GGGGAGGCCG GCCCACTGGG 
     4561 CCTCAGGCCC CAAAGTGGAC AACCGCGCGG TCAGGAGCAT CAATGAGGCC CGCTACGCCG 
     4621 GCAAGGAGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4681 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4741 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4801 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4861 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4921 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4981 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5041 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5101 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5161 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5221 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5281 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5341 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5401 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5461 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5521 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5581 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5641 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5701 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5761 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5821 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5881 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5941 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6001 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6061 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6121 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6181 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6241 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6301 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6361 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6421 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6481 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6541 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6601 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6661 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6721 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6781 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6841 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6901 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6961 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7021 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7081 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7141 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7201 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7261 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7321 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7381 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7441 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7501 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7561 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7621 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7681 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7741 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7801 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7861 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7921 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7981 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8041 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8101 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8161 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8221 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8281 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8341 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8401 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8461 CAAAAATTTA ACGCGAATTT T
//