LOCUS       RC221017                8574 bp    DNA     circular  SYN 
DEFINITION  STAG2 (Myc-DDK-tagged)-Human stromal antigen 2 (STAG2), transcript variant 3
ACCESSION   NM_001042751
VERSION     NM_001042751.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4731..4822 
                     /label="Myc-DDK"  
     misc_feature    4852..5437 
                     /label="PolyA signal"  
     misc_feature    4909..4928 
                     /label="Restriction Enzyme"  
     misc_feature    5713..6385 
                     /label="ColE1"  
     misc_feature    6815..7609 
                     /label="Kan/Neo"  
     misc_feature    7644..7946 
                     /label="SV40 ori"  
     misc_feature    8032..8509 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4713..4719 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4713 
                     /label="STAG2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATAGCAGCT CCAGAAATAC CAACTGATTT TAATCTACTA CAGGAGTCAG 
     1081 AAACACATTT TTCTTCTGAC ACAGATTTTG AAGATATCGA AGGAAAAAAC CAAAAGCAAG 
     1141 GCAAAGGCAA AACTTGTAAA AAAGGCAAAA AGGGCCCAGC AGAAAAGGGC AAAGGTGGAA 
     1201 ATGGAGGAGG AAAACCTCCT TCTGGTCCAA ACCGAATGAA TGGTCATCAC CAACAGAATG 
     1261 GAGTGGAAAA CATGATGTTG TTTGAAGTTG TTAAAATGGG CAAGAGTGCT ATGCAGTCGG 
     1321 TGGTAGATGA TTGGATAGAA TCATACAAGC ATGACCGAGA TATAGCACTT CTTGACCTTA 
     1381 TCAACTTTTT TATTCAGTGT TCAGGCTGTA AAGGAGTTGT CACAGCAGAA ATGTTTAGAC 
     1441 ATATGCAGAA CTCTGAGATA ATTCGAAAAA TGACTGAAGA ATTCGATGAG GATAGTGGAG 
     1501 ATTATCCACT TACCATGGCT GGTCCTCAGT GGAAGAAGTT CAAATCCAGT TTTTGTGAAT 
     1561 TCATTGGCGT GTTAGTACGG CAATGTCAAT ATAGTATCAT ATATGATGAG TATATGATGG 
     1621 ATACAGTCAT TTCACTTCTT ACAGGATTGT CTGACTCACA AGTCAGAGCA TTTCGACATA 
     1681 CAAGCACCCT GGCAGCTATG AAGTTGATGA CAGCTTTGGT GAATGTGGCA CTAAATCTTA 
     1741 GCATTAATAT GGATAATACA CAAAGACAAT ATGAAGCAGA ACGGAATAAA ATGATTGGAA 
     1801 AACGAGCCAA TGAGAGGCTA GAACTCCTGC TACAAAAGCG GAAAGAGCTT CAGGAAAATC 
     1861 AAGATGAAAT AGAAAATATG ATGAATGCAA TATTTAAAGG AGTGTTTGTA CATAGATACC 
     1921 GTGATGCGAT AGCTGAAATT CGAGCTATTT GCATTGAAGA GATTGGCATT TGGATGAAGA 
     1981 TGTATAGTGA TGCCTTTCTT AATGACAGTT ATTTAAAATA TGTTGGTTGG ACTATGCATG 
     2041 ATAAGCAAGG TGAAGTAAGA CTCAAATGTC TTACTGCTCT ACAAGGGCTT TATTATAACA 
     2101 AAGAGCTTAA TTCCAAACTG GAACTTTTTA CCAGTCGGTT CAAGGATAGA ATTGTGTCTA 
     2161 TGACCCTTGA CAAAGAATAT GATGTTGCAG TACAAGCAAT AAAATTACTC ACTCTTGTTT 
     2221 TACAGAGTAG TGAAGAAGTT CTCACTGCAG AAGATTGTGA AAATGTCTAT CATCTGGTTT 
     2281 ATTCAGCTCA CCGGCCAGTA GCAGTAGCAG CTGGAGAATT TCTCTACAAA AAGCTCTTCA 
     2341 GTCGTAGAGA TCCAGAGGAG GATGGAATGA TGAAAAGAAG AGGAAGACAA GGTCCAAATG 
     2401 CCAACCTTGT TAAGACATTG GTTTTTTTCT TTCTAGAAAG TGAGTTACAT GAGCATGCAG 
     2461 CATACCTTGT GGATAGCATG TGGGACTGTG CTACTGAGCT GCTGAAAGAC TGGGAATGTA 
     2521 TGAATAGCTT GTTACTGGAA GAGCCACTTA GTGGAGAGGA AGCACTAACA GATAGGCAAG 
     2581 AGAGTGCTCT GATTGAAATA ATGCTTTGTA CCATTAGACA AGCGGCTGAA TGTCATCCTC 
     2641 CCGTGGGAAG AGGGACAGGA AAAAGGGTGC TTACAGCAAA GGAGAAGAAG ACACAGTTGG 
     2701 ATGATAGGAC AAAAATCACT GAGCTTTTTG CCGTGGCCCT TCCTCAGTTA TTAGCAAAAT 
     2761 ACTCTGTAGA TGCAGAAAAG GTGACTAACT TGTTGCAGTT GCCTCAGTAC TTTGATTTGG 
     2821 AAATATATAC CACTGGACGA TTAGAAAAGC ATTTGGATGC CTTATTGCGA CAGATCCGGA 
     2881 ATATTGTAGA GAAGCACACA GATACAGATG TTTTGGAAGC ATGTTCTAAA ACTTACCATG 
     2941 CACTCTGTAA TGAAGAGTTC ACAATCTTCA ACAGAGTAGA TATTTCAAGA AGTCAACTGA 
     3001 TAGATGAATT GGCAGATAAA TTTAACCGGC TTCTTGAAGA TTTTCTGCAA GAGGGTGAAG 
     3061 AACCTGATGA AGATGATGCA TATCAGGTAT TGTCAACATT GAAGAGGATC ACTGCTTTTC 
     3121 ATAATGCCCA TGACCTTTCA AAGTGGGATT TATTTGCTTG TAATTACAAA CTCTTGAAAA 
     3181 CTGGAATCGA AAATGGAGAC ATGCCTGAGC AGATTGTTAT TCACGCACTG CAGTGTACTC 
     3241 ACTATGTAAT CCTTTGGCAA CTTGCTAAGA TAACTGAAAG CAGCTCTACA AAGGAGGACT 
     3301 TGCTGCGTTT AAAGAAACAA ATGAGAGTAT TTTGTCAGAT ATGTCAACAT TACCTGACCA 
     3361 ACGTGAATAC TACTGTTAAG GAACAGGCCT TCACTATTCT GTGTGATATT TTGATGATCT 
     3421 TCAGCCATCA GATTATGTCA GGAGGGCGTG ACATGTTAGA GCCATTAGTG TATACCCCTG 
     3481 ATTCTTCATT GCAGTCTGAG TTGCTCAGCT TTATTTTGGA TCATGTCTTC ATTGAACAGG 
     3541 ATGATGATAA TAATAGTGCA GATGGTCAGC AAGAGGATGA AGCCAGTAAA ATTGAAGCTC 
     3601 TGCACAAGAG AAGAAATTTA CTTGCAGCAT TTTGTAAGCT AATTGTATAT ACTGTGGTGG 
     3661 AGATGAATAC AGCTGCAGAT ATCTTCAAAC AGTATATGAA GTATTATAAT GACTATGGAG 
     3721 ATATCATCAA AGAAACAATG AGTAAAACAA GGCAGATAGA CAAAATTCAG TGTGCTAAGA 
     3781 CCCTTATTCT CAGTCTGCAA CAGCTTTTTA ATGAAATGAT ACAAGAAAAT GGCTATAATT 
     3841 TTGATAGATC ATCCTCTACA TTTAGTGGCA TAAAAGAACT TGCTCGACGT TTTGCTTTAA 
     3901 CTTTTGGACT TGATCAGTTG AAAACAAGAG AAGCCATTGC CATGCTACAC AAAGATGGCA 
     3961 TAGAATTTGC TTTTAAAGAG CCTAATCCGC AAGGGGAGAG CCATCCACCT TTAAATTTGG 
     4021 CATTTCTTGA TATTCTGAGT GAATTTTCTT CTAAACTACT TCGACAAGAC AAAAGAACAG 
     4081 TGTATGTTTA CTTGGAAAAG TTCATGACCT TTCAGATGTC ACTCCGAAGA GAGGATGTGT 
     4141 GGCTTCCACT GATGTCTTAC CGAAATTCTT TGCTAGCTGG TGGTGATGAT GACACCATGT 
     4201 CAGTCATTAG TGGAATCAGC AGCCGGGGGT CAACAGTACG GAGTAAAAAA TCAAAACCAT 
     4261 CTACAGGAAA ACGGAAAGTG GTTGAGGGCA TGCAGCTTTC ACTCACTGAA GAAAGTAGTA 
     4321 GTAGTGACAG TATGTGGTTA AGCAGAGAAC AAACACTGCA CACCCCTGTT ATGATGCAGA 
     4381 CACCACAACT CACCTCCACT ATTATGAGAG AGCCCAAAAG ATTACGGCCT GAGGATAGCT 
     4441 TCATGAGTGT TTATCCAATG CAGACTGAAC ATCATCAAAC ACCTCTTGAT TATAATCGGC 
     4501 GTGGCACAAG CCTAATGGAA GATGATGAAG AGCCAATTGT GGAAGATGTT ATGATGTCCT 
     4561 CAGAAGGGAG GATTGAGGAT CTTAATGAGG GAATGGATTT TGACACCATG GATATAGATT 
     4621 TGCCACCATC AAAGAACAGA CGAGAGAGAA CAGAACTGAA GCCTGATTTC TTTGATCCAG 
     4681 CTTCAATTAT GGATGAATCA GTTCTTGGAG TGTCAATGTT TACGCGTACG CGGCCGCTCG 
     4741 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4801 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4861 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4921 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4981 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5041 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5101 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5161 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5221 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5281 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5341 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5401 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5461 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5521 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5581 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5641 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5701 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5761 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5821 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5881 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5941 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6001 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6061 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6121 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6181 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6241 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6301 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6361 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6421 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6481 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6541 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6601 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6661 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6721 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6781 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6841 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6901 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6961 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7021 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7081 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7141 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7201 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7261 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7321 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7381 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7441 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7501 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7561 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7621 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7681 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7741 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7801 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7861 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7921 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7981 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8041 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8101 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8161 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8221 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8281 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8341 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8401 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8461 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8521 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//