LOCUS       RC220992                7920 bp    DNA     circular  SYN 
DEFINITION  RTN3 (Myc-DDK-tagged)-Human reticulon 3 (RTN3), transcript variant 2
ACCESSION   NM_201428
VERSION     NM_201428.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4077..4168 
                     /label="Myc-DDK"  
     misc_feature    4198..4783 
                     /label="PolyA signal"  
     misc_feature    4255..4274 
                     /label="Restriction Enzyme"  
     misc_feature    5059..5731 
                     /label="ColE1"  
     misc_feature    6161..6955 
                     /label="Kan/Neo"  
     misc_feature    6990..7292 
                     /label="SV40 ori"  
     misc_feature    7378..7855 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4059..4065 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4059 
                     /label="RTN3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGCCG TCGGCGGCCA CTCAGTCCCA TTCCATCTCC TCGTCGTCCT 
     1081 TCGGAGCCGA GCCGTCCGCG CCCGGCGGCG GCGGGAGCCC AGGAGCCTGC CCCGCCCTGG 
     1141 GGACGAAGAG CTGCAGCTCC TCCTGTGCGG AGGGATTGAG CTCTCTTTGC TCTGATGAGC 
     1201 CATCTTCAGA AATTATGACT TCTTCCTTTC TTTCATCTTC TGAAATACAT AACACTGGCC 
     1261 TTACAATACT ACATGGAGAA AAAAGCCATG TGTTAGGGAG CCAGCCTATT TTAGCCAAAG 
     1321 AAGGAAAAGA CCACTTGGAT CTTCTAGATA TGAAAAAGAT GGAAAAGCCT CAGGGGACCA 
     1381 GCAACAACGT ATCAGACTCT TCAGTTTCTC TTGCAGCAGG AGTTCATTGT GACCGTCCTT 
     1441 CTATTCCAGC CAGTTTCCCA GAGCATCCTG CTTTTCTCTC AAAGAAAATT GGTCAAGTGG 
     1501 AAGAGCAAAT AGATAAAGAG ACCAAGAACC CAAATGGGGT ATCAAGTAGG GAGGCTAAAA 
     1561 CTGCATTGGA TGCTGATGAC AGATTCACTT TGCTGACAGC CCAGAAACCA CCTACTGAGT 
     1621 ACTCTAAGGT AGAAGGCATT TATACATATT CTTTGTCTCC ATCCAAAGTT TCAGGAGATG 
     1681 ATGTTATTGA AAAGGATTCC CCTGAATCAC CATTTGAAGT AATTATTGAC AAAGCAGCAT 
     1741 TTGACAAAGA ATTTAAAGAC TCATATAAGG AGAGCACAGA TGATTTTGGT AGCTGGTCTG 
     1801 TGCACACTGA TAAAGAATCA TCCGAAGACA TTTCAGAGAC TAATGACAAG CTTTTTCCAC 
     1861 TGAGAAATAA AGAGGCAGGA CGTTACCCAA TGTCTGCATT GCTCAGTAGG CAGTTTTCAC 
     1921 ACACAAATGC AGCACTGGAA GAGGTGTCCA GATGCGTGAA TGATATGCAT AACTTTACTA 
     1981 ACGAAATACT GACTTGGGAT CTGGTTCCCC AAGTGAAACA ACAGACCGAT AAATCTTCTG 
     2041 ACTGCATCAC AAAAACTACA GGACTTGACA TGAGTGAATA TAATTCAGAA ATTCCAGTTG 
     2101 TAAATCTTAA AACTAGCACT CATCAGAAAA CTCCTGTATG TTCTATTGAT GGGAGCACTC 
     2161 CCATCACTAA ATCAACAGGT GATTGGGCAG AAGCATCTCT CCAGCAAGAA AATGCTATTA 
     2221 CTGGAAAACC TGTACCTGAC TCTTTGAATT CCACAAAAGA ATTCAGTATC AAAGGTGTGC 
     2281 AAGGCAATAT GCAGAAACAG GATGACACAC TTGCAGAATT ACCTGGATCT CCACCTGAGA 
     2341 AATGTGACTC TTTGGGTTCT GGAGTGGCCA CAGTGAAAGT GGTTTTACCT GATGACCACC 
     2401 TGAAAGATGA AATGGACTGG CAGAGCTCTG CATTGGGAGA AATCACAGAA GCTGATAGTT 
     2461 CTGGTGAGTC TGATGACACA GTAATAGAGG ACATCACAGC AGATACATCA TTTGAAAATA 
     2521 ACAAAATTCA GGCTGAAAAA CCTGTTTCCA TTCCAAGTGC TGTTGTAAAA ACAGGTGAAA 
     2581 GAGAAATCAA AGAGATTCCC AGTTGTGAGA GAGAAGAAAA AACATCTAAA AACTTTGAAG 
     2641 AATTGGTCAG TGACTCTGAG CTGCATCAAG ATCAGCCTGA TATTCTTGGA AGGAGTCCAG 
     2701 CTAGTGAGGC AGCATGTTCA AAAGTACCCG ATACGAATGT CTCCTTAGAA GATGTGAGTG 
     2761 AAGTTGCTCC TGAAAAGCCT ATTACTACTG AGAACCCCAA ACTTCCTTCA ACAGTGTCTC 
     2821 CAAATGTTTT TAATGAGACA GAATTCTCAT TAAATGTGAC AACATCTGCC TATTTGGAGT 
     2881 CATTACATGG GAAAAATGTT AAACATATAG ATGATTCCTC CCCAGAGGAC CTGATAGCAG 
     2941 CCTTTACAGA AACCAGAGAT AAAGGAATAG TAGATAGTGA AAGAAATGCT TTTAAAGCAA 
     3001 TATCAGAGAA GATGACAGAC TTTAAAACAA CTCCTCCTGT AGAAGTCTTA CATGAAAATG 
     3061 AGTCCGGTGG TTCTGAAATT AAAGACATTG GAAGCAAATA CAGTGAACAA AGCAAAGAAA 
     3121 CAAATGGAAG TGAGCCTCTA GGTGTTTTCC CTACCCAAGG TACTCCAGTA GCATCTCTTG 
     3181 ACTTAGAACA AGAACAGCTC ACAATTAAGG CTCTTAAAGA ATTAGGTGAA AGACAGGTTG 
     3241 AGAAGTCAAC TTCTGCACAG CGTGACGCAG AATTGCCTTC TGAAGAAGTA CTGAAGCAAA 
     3301 CTTTCACATT TGCTCCAGAA TCTTGGCCAC AGAGATCATA TGACATCCTA GAACGTAATG 
     3361 TCAAGAATGG ATCTGATCTT GGGATTTCCC AGAAGCCCAT CACTATCAGA GAAACTACTA 
     3421 GGGTAGATGC TGTTTCCAGC CTTAGCAAGA CTGAATTGGT AAAAAAGCAT GTCCTAGCAA 
     3481 GACTTCTGAC AGACTTCTCA GTGCACGATC TGATTTTCTG GAGAGATGTG AAGAAGACTG 
     3541 GGTTTGTCTT TGGCACCACG CTGATCATGC TGCTTTCCCT GGCAGCTTTC AGTGTCATCA 
     3601 GTGTGGTTTC TTACCTCATC CTGGCTCTTC TCTCTGTCAC CATCAGCTTC AGGATCTACA 
     3661 AGTCCGTCAT CCAAGCTGTA CAGAAGTCAG AAGAAGGCCA TCCATTCAAA GCCTACCTGG 
     3721 ACGTAGACAT TACTCTGTCC TCAGAAGCTT TCCATAATTA CATGAATGCT GCCATGGTGC 
     3781 ACATCAACAG GGCCCTGAAA CTCATTATTC GTCTCTTTCT GGTAGAAGAT CTGGTTGACT 
     3841 CCTTGAAGCT GGCTGTCTTC ATGTGGCTGA TGACCTATGT TGGTGCTGTT TTTAACGGAA 
     3901 TCACCCTTCT AATTCTTGCT GAACTGCTCA TTTTCAGTGT CCCGATTGTC TATGAGAAGT 
     3961 ACAAGACCCA GATTGATCAC TATGTTGGCA TCGCCCGAGA TCAGACCAAG TCAATTGTTG 
     4021 AAAAGATCCA AGCAAAACTC CCTGGAATCG CCAAAAAAAA GGCAGAAACG CGTACGCGGC 
     4081 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4141 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4201 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4261 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4321 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4381 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4441 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4501 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4561 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4621 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4681 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4741 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4801 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4861 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4921 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4981 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5041 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5101 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5161 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5221 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5281 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5341 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5401 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5461 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5521 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5581 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5641 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5701 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5761 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5821 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5881 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5941 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6001 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6061 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6121 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6181 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6241 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6301 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6361 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6421 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6481 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6541 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6601 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6661 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6721 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6781 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6841 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6901 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6961 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7021 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7081 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7141 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7201 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7261 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7321 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7381 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7441 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7501 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7561 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7621 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7681 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7741 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7801 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7861 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//