LOCUS       RC220966                8523 bp    DNA     circular  SYN 
DEFINITION  IGSF3 (Myc-DDK-tagged)-Human immunoglobulin superfamily, member 3 (IGSF3), transcript variant 1
ACCESSION   NM_001542
VERSION     NM_001542.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4680..4771 
                     /label="Myc-DDK"  
     misc_feature    4801..5386 
                     /label="PolyA signal"  
     misc_feature    4858..4877 
                     /label="Restriction Enzyme"  
     misc_feature    5662..6334 
                     /label="ColE1"  
     misc_feature    6764..7558 
                     /label="Kan/Neo"  
     misc_feature    7593..7895 
                     /label="SV40 ori"  
     misc_feature    7981..8458 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4662..4668 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4662 
                     /label="IGSF3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGTGCTTT TTCCCGGTGC TGAGCTGTCT GGCTGTGCTG GGTGTGGTGT 
     1081 CAGCACAGCG GCAGGTCACC GTTCAGGAAG GACCCTTGTA CCGCACGGAG GGCTCCCACA 
     1141 TCACTATCTG GTGCAATGTG AGTGGCTACC AGGGACCTTC TGAGCAGAAT TTCCAGTGGT 
     1201 CCATTTACCT GCCTTCGTCG CCAGAGCGAG AGGTGCAGAT CGTCAGCACC ATGGACTCTT 
     1261 CCTTCCCCTA TGCCATCTAC ACCCAGCGCG TCCGCGGAGG GAAGATCTTC ATAGAAAGAG 
     1321 TCCAGGGGAA CTCAACCCTA TTGCACATCA CAGATCTTCA GGCCCGGGAT GCCGGGGAGT 
     1381 ATGAATGCCA CACACCCAGC ACTGATAAGC AATACTTTGG GAGTTACAGT GCAAAGATGA 
     1441 ACCTAGTGGT GATCCCAGAC TCCCTGCAGA CCACTGCCAT GCCCCAGACT CTGCACAGAG 
     1501 TGGAGCAGGA CCCGCTGGAG CTCACTTGTG AGGTGGCCTC AGAGACCATT CAGCACAGCC 
     1561 ACCTGTCTGT GGCCTGGCTC CGGCAGAAAG TTGGCGAGAA GCCCGTGGAG GTCATCTCCC 
     1621 TGAGCCGAGA TTTCATGCTT CACTCCAGCA GCGAATATGC CCAGAGGCAG AGCCTGGGGG 
     1681 AGGTGCGGCT GGACAAGCTG GGGAGGACCA CCTTCCGCCT CACCATCTTC CACCTGCAGC 
     1741 CTTCTGACCA GGGCGAATTC TACTGCGAGG CCGCCGAGTG GATCCAGGAT CCGGATGGGT 
     1801 CGTGGTATGC TATGACCCGA AAGCGTTCCG AGGGAGCCGT GGTCAACGTC CAGCCAACTG 
     1861 ACAAAGAATT CACTGTTCGG CTGGAGACAG AGAAGCGGCT GCACACGGTG GGCGAGCCGG 
     1921 TGGAGTTCAG ATGCATCCTG GAGGCTCAGA ATGTTCCCGA CCGTTACTTT GCTGTCTCCT 
     1981 GGGCCTTCAA CAGCTCGCTC ATCGCCACCA TGGGTCCTAA CGCTGTGCCT GTCCTCAACA 
     2041 GCGAATTTGC TCACCGGGAA GCCAGGGGAC AGCTTAAGGT GGCCAAAGAG AGCGACAGTG 
     2101 TCTTTGTGCT GAAGATCTAC CACCTCCGCC AGGAAGATAG CGGGAAATAC AACTGCCGGG 
     2161 TGACTGAGCG AGAGAAAACC GTGACCGGGG AATTCATTGA TAAGGAGAGC AAGCGTCCCA 
     2221 AGAACATCCC CATCATAGTC CTCCCCCTCA CAGATAACTG GGTAGTTAAA GTCCCCCAGC 
     2281 ACCACCAGCT CCTGTCCCAA GGCCACTTGG AGAGCAGCAT CTCCGTGGAG GTGGCCAGCA 
     2341 ATGCCAGCGT CATCCTTGAG GGCGAGGACC TGCGCTTCTC CTGCAGTGTC CGCACGGCAG 
     2401 GCAGGCCGCA GGGTCGCTTC TCTGTCATCT GGCAGCTTGT GGACAGGCAG AACCGCCGCA 
     2461 GCAATATCAT GTGGCTAGAC CGGGATGGCA CCGTGCAGCC AGGCTCGTCC TACTGGGAGC 
     2521 GCAGCAGCTT TGGGGGCGTC CAGATGGAGC AGGTGCAGCC CAACTCGTTC AGCCTGGGCA 
     2581 TCTTCAACAG CAGGAAGGAG GACGAGGGCC AGTATGAATG CCATGTGACT GAATGGGTGC 
     2641 GGGCAGTGGA TGGCGAGTGG CAGATTGTTG GGGAGCGCCG GGCCAGCACT CCCATCTCCA 
     2701 TCACAGCTCT TGAAATGGGC TTCGCAGTCA CAGCCATCTC CCGGACACCG GGGGTGACCT 
     2761 ACAGCGACTC CTTTGACTTG CAGTGTATCA TCAAACCCCA CTACCCTGCC TGGGTCCCCG 
     2821 TGTCGGTGAC ATGGCGGTTC CAGCCGGTGG GCACGGTGGA GTTCCATGAC TTGGTGACCT 
     2881 TCACCCGGGA CGGAGGGGTC CAGTGGGGGG ACAGGTCCTC CAGCTTCCGA ACCCGAACTG 
     2941 CCATCGAGAA GGCTGAGTCC AGCAACAACG TCCGCCTAAG CATCAGCCGA GCCAGTGACA 
     3001 CGGAAGCAGG CAAGTACCAG TGTGTGGCAG AGCTGTGGCG GAAGAACTAC AACAACACCT 
     3061 GGACGCGACT GGCGGAGAGG ACCTCCAACC TGCTGGAGAT CAGGGTGCTG CAGCCAGTGA 
     3121 CAAAGCTGCA GGTGAGCAAA TCGAAGAGGA CCCTCACCCT GGTGGAAAAC AAGCCCATTC 
     3181 AGTTGAACTG CTCAGTCAAG TCTCAGACTA GCCAGAACTC CCACTTTGCG GTGCTCTGGT 
     3241 ATGTCCACAA GCCCTCGGAT GCCGATGGCA AGCTTATCCT GAAGACCACC CACAACTCCG 
     3301 CCTTTGAATA CGGTACTTAC GCCGAGGAGG AGGGCCTGAG AGCCAGGCTC CAGTTTGAGA 
     3361 GGCATGTGTC GGGGGGCCTG TTCAGCCTCA CCGTCCAGAG AGCCGAGGTC AGCGACAGCG 
     3421 GCAGCTACTA CTGCCACGTG GAGGAGTGGC TGCTGAGCCC CAACTACGCC TGGTACAAGC 
     3481 TGGCAGAGGA GGTTTCTGGG CGCACAGAAG TCACTGTGAA ACAGCCAGAC AGCCGCCTGA 
     3541 GGCTCAGCCA AGCCCAGGGG AACCTGTCGG TTCTGGAGAC CCGGCAGGTA CAGCTGGAGT 
     3601 GTGTGGTTCT CAACCGCACC AGCATAACCT CCCAGCTCAT GGTGGAATGG TTTGTATGGA 
     3661 AGCCCAACCA CCCTGAGCGG GAGACTGTGG CCCGCTTGAG CCGTGACGCC ACCTTCCACT 
     3721 ATGGAGAGCA GGCAGCCAAG AACAATCTGA AGGGGCGGCT GCATTTGGAG AGTCCTTCCC 
     3781 CCGGCGTGTA CCGTCTCTTC ATCCAGAACG TGGCTGTGCA GGACAGCGGG ACCTACAGCT 
     3841 GCCATGTGGA GGAGTGGCTG CCCAGCCCCA GTGGCATGTG GTATAAGCGG GCAGAGGACA 
     3901 CCGCTGGGCA GACAGCTCTG ACAGTCATGC GACCAGATGC TTCCCTGCAG GTGGACACAG 
     3961 TGGTCCCCAA TGCCACGGTC TCTGAGAAGG CAGCTTTCCA GCTGGACTGT AGCATCGTGT 
     4021 CCCGCTCCAG CCAGGACTCC CGCTTCGCTG TGGCCTGGTA TTCCCTGAGG ACTAAAGCTG 
     4081 GGGGGAAAAG GAGCAGCCCT GGCCTGGAAG AACAGGAAGA GGAAAGGGAG GAGGAGGAGG 
     4141 AGGAGGACGA CGACGACGAC GACGACCCAA CAGAGCGGAC GGCCCTGCTG AGCGTGGGCC 
     4201 CAGATGCTGT CTTTGGCCCA GAGGGCAGTC CTTGGGAGGG CAGGCTTCGC TTCCAGAGGC 
     4261 TCTCCCCGGT GCTCTACCGG CTCACAGTGC TGCAGGCAAG CCCCCAAGAT ACAGGCAATT 
     4321 ACTCCTGCCA TGTGGAGGAG TGGCTGCCCA GCCCTCAGAA GGAATGGTAC CGGCTGACGG 
     4381 AGGAGGAGTC AGCCCCCATC GGCATCCGTG TTCTAGATAC AAGTCCCACC CTCCAGTCCA 
     4441 TCATCTGCTC CAACGACGCA CTCTTCTACT TCGTCTTCTT CTACCCTTTC CCCATCTTTG 
     4501 GCATTCTTAT CATCACCATC CTTCTGGTGC GTTTCAAGAG CCGGAACTCC AGCAAGAACT 
     4561 CTGATGGGAA GAATGGGGTG CCTCTGCTGT GGATCAAAGA GCCACACCTC AACTACTCCC 
     4621 CTACTTGCCT GGAGCCCCCT GTTCTCAGTA TCCATCCAGG GGCCATAGAC ACGCGTACGC 
     4681 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4741 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4801 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4861 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4921 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4981 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5041 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5101 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5161 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5221 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5281 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5341 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5401 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5461 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5521 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5581 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5641 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5701 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5761 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5821 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5881 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5941 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6001 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6061 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6121 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6181 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6241 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6301 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6361 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6421 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6481 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6541 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6601 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6661 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6721 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6781 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6841 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6901 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6961 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7021 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7081 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7141 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7201 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7261 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7321 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7381 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7441 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7501 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7561 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7621 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7681 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7741 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7801 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7861 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7921 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7981 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8041 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8101 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8161 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8221 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8281 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8341 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8401 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8461 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8521 TTT
//