LOCUS       RC220941                7188 bp    DNA     circular  SYN 
DEFINITION  ADAM11 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 11 (ADAM11)
ACCESSION   NM_002390
VERSION     NM_002390.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3345..3436 
                     /label="Myc-DDK"  
     misc_feature    3466..4051 
                     /label="PolyA signal"  
     misc_feature    3523..3542 
                     /label="Restriction Enzyme"  
     misc_feature    4327..4999 
                     /label="ColE1"  
     misc_feature    5429..6223 
                     /label="Kan/Neo"  
     misc_feature    6258..6560 
                     /label="SV40 ori"  
     misc_feature    6646..7123 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3327..3333 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3327 
                     /label="ADAM11"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGCTGCTG CGGCGCTGGG CGTTCGCGGC TCTGCTGCTG TCGCTGCTCC 
     1081 CCACGCCCGG TCTTGGGACC CAAGGTCCTG CTGGAGCTCT GCGATGGGGG GGCTTACCCC 
     1141 AGCTGGGAGG CCCAGGAGCC CCTGAGGTCA CGGAACCCAG CCGTCTGGTT AGGGAGAGCT 
     1201 CCGGGGGAGA GGTCCGAAAG CAGCAGCTGG ACACAAGGGT CCGCCAGGAG CCACCAGGGG 
     1261 GCCCGCCTGT CCATCTGGCC CAGGTGAGTT TCGTCATCCC AGCCTTCAAC TCAAACTTCA 
     1321 CCCTGGACCT GGAGCTGAAC CACCACCTCC TCTCCTCGCA ATACGTGGAG CGCCACTTCA 
     1381 GCCGGGAGGG GACAACCCAG CACAGCACCG GGGCTGGAGA CCACTGCTAC TACCAGGGGA 
     1441 AGCTCCGGGG GAACCCGCAC TCCTTCGCCG CCCTCTCCAC CTGCCAGGGG CTGCATGGGG 
     1501 TCTTCTCTGA TGGGAACTTG ACTTACATCG TGGAGCCCCA AGAGGTGGCT GGACCTTGGG 
     1561 GAGCCCCTCA GGGACCCCTT CCCCACCTCA TTTACCGGAC CCCTCTCCTC CCAGATCCCC 
     1621 TCGGATGCAG GGAACCAGGC TGCCTGTTTG CTGTGCCTGC CCAGTCGGCT CCTCCAAACC 
     1681 GGCCGAGGCT GAGAAGGAAA AGGCAGGTCC GCCGGGGCCA CCCTACAGTG CACAGTGAAA 
     1741 CCAAGTATGT GGAGCTAATT GTGATCAACG ACCACCAGCT GTTCGAGCAG ATGCGACAGT 
     1801 CGGTGGTCCT CACCAGCAAC TTTGCCAAGT CCGTGGTGAA CCTGGCCGAT GTGATATACA 
     1861 AGGAGCAGCT CAACACTCGC ATCGTCCTGG TTGCCATGGA AACATGGGCA GATGGGGACA 
     1921 AGATCCAGGT GCAGGATGAC CTCCTGGAGA CCCTGGCCCG GCTCATGGTC TACCGACGGG 
     1981 AGGGTCTGCC TGAGCCCAGT GATGCCACCC ACCTCTTCTC GGGCAGGACC TTCCAGAGCA 
     2041 CGAGCAGCGG GGCAGCCTAC GTGGGGGGCA TATGCTCCCT GTCCCACGGC GGGGGTGTGA 
     2101 ACGAGTACGG CAACATGGGG GCGATGGCCG TGACCCTTGC CCAGACGCTG GGACAGAACC 
     2161 TGGGCATGAT GTGGAACAAA CACCGGAGCT CGGCAGGGGA CTGCAAGTGT CCAGACATCT 
     2221 GGCTGGGCTG CATCATGGAG GACACTGGGT TCTACCTGCC CCGCAAGTTC TCGCGCTGTA 
     2281 GCATCGACGA GTACAACCAG TTTCTGCAGG AGGGTGGTGG CAGCTGCCTC TTCAACAAGC 
     2341 CCCTCAAGCT CCTGGACCCC CCAGAGTGCG GGAACGGCTT CGTGGAGGCA GGGGAGGAGT 
     2401 GCGACTGCGG CTCGGTGCAG GAGTGCAGCC GCGCAGGTGG CAACTGCTGC AAGAAATGCA 
     2461 CCCTGACTCA CGACGCCATG TGCAGCGACG GGCTCTGCTG TCGCCGCTGC AAGTACGAAC 
     2521 CACGGGGTGT GTCCTGCCGA GAGGCCGTGA ACGAGTGCGA CATCGCGGAG ACCTGCACCG 
     2581 GGGACTCTAG CCAGTGCCCG CCTAACCTGC ACAAGCTGGA CGGTTACTAC TGTGACCATG 
     2641 AGCAGGGCCG CTGCTACGGA GGTCGCTGCA AAACCCGGGA CCGGCAGTGC CAGGTTCTTT 
     2701 GGGGCCATGC GGCTGCTGAT CGCTTCTGCT ACGAGAAGCT GAATGTGGAG GGGACGGAGC 
     2761 GTGGGAGCTG TGGGCGCAAG GGATCTGGCT GGGTCCAGTG CAGTAAGCAG GACGTGCTGT 
     2821 GTGGCTTCCT CCTCTGTGTC AACATCTCTG GAGCTCCTCG GCTAGGGGAC CTGGTGGGAG 
     2881 ACATCAGTAG TGTCACCTTC TACCACCAGG GCAAGGAGCT GGACTGCAGG GGAGGCCACG 
     2941 TGCAGCTGGC GGACGGCTCT GACCTGAGCT ATGTGGAGGA TGGCACAGCC TGCGGGCCTA 
     3001 ACATGTTGTG CCTGGACCAT CGCTGCCTGC CAGCTTCTGC CTTCAACTTC AGCACCTGCC 
     3061 CCGGCAGTGG GGAGCGCCGG ATTTGCTCCC ACCACGGGGT CTGCAGCAAT GAAGGGAAGT 
     3121 GCATCTGTCA GCCAGACTGG ACAGGCAAAG ACTGCAGTAT CCATAACCCC CTGCCCACGT 
     3181 CCCCACCCAC GGGGGAGACG GAGAGATATA AAGGTCCCAG CGGCACCAAC ATCATCATTG 
     3241 GCTCCATTGC TGGGGCTGTC CTGGTTGCAG CCATCGTCCT GGGCGGCACG GGCTGGGGAT 
     3301 TTAAAAACAT TCGCCGAGGA AGGTCCGGAG GGGCCACGCG TACGCGGCCG CTCGAGCAGA 
     3361 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3421 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3481 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3541 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3601 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3661 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3721 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3781 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3841 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     3901 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     3961 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4021 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4081 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4141 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4201 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4261 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4321 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4381 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4441 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4501 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4561 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4621 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4681 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4741 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4801 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     4861 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     4921 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     4981 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5041 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5101 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5161 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5221 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5281 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5341 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5401 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5461 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5521 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5581 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5641 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5701 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5761 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     5821 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     5881 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     5941 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6001 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6061 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6121 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6181 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6241 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6301 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6361 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6421 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6481 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6541 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6601 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6661 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6721 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6781 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     6841 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     6901 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     6961 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7021 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7081 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7141 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//