LOCUS       RC220918                6855 bp    DNA     circular  SYN 
DEFINITION  AGPS (Myc-DDK-tagged)-Human alkylglycerone phosphate synthase (AGPS)
ACCESSION   NM_003659
VERSION     NM_003659.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3012..3103 
                     /label="Myc-DDK"  
     misc_feature    3133..3718 
                     /label="PolyA signal"  
     misc_feature    3190..3209 
                     /label="Restriction Enzyme"  
     misc_feature    3994..4666 
                     /label="ColE1"  
     misc_feature    5096..5890 
                     /label="Kan/Neo"  
     misc_feature    5925..6227 
                     /label="SV40 ori"  
     misc_feature    6313..6790 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2994..3000 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2994 
                     /label="AGPS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGGCG GCGGCTGCAG CGGGTGGGAC TGGCTTGGGC GCGGGCGCGA 
     1081 GCTACGGGTC TGCAGCGGAC CGGGACCGGG ACCCGGACCC GGACCGCGCC GGGCGGAGGC 
     1141 TGCGGGTTCT CTCTGGCCAT CTGCTGGGCC GGCCCCGGGA GGCTCTGAGT ACCAATGAGT 
     1201 GCAAAGCGCG GAGAGCCGCG TCGGCGGCCA CGGCAGCGCC CACGGCCACT CCCGCCGCGC 
     1261 AGGAGTCGGG CACCATCCCA AAGAAGCGGC AAGAAGTTAT GAAATGGAAT GGATGGGGAT 
     1321 ATAATGATTC TAAATTCATC TTCAATAAGA AGGGCCAAAT TGAATTGACT GGGAAAAGGT 
     1381 ACCCTCTTAG TGGCATGGGT TTACCAACAT TTAAAGAATG GATCCAAAAT ACCCTTGGAG 
     1441 TAAATGTGGA GCATAAAACT ACCTCTAAAG CATCCTTAAA TCCTAGTGAT ACACCTCCTT 
     1501 CTGTTGTAAA TGAAGATTTT CTTCATGACC TTAAAGAAAC TAATATTTCA TATTCACAAG 
     1561 AGGCAGATGA TCGAGTATTT AGAGCTCATG GTCATTGTCT TCATGAGATA TTTTTGCTCA 
     1621 GGGAAGGAAT GTTTGAGCGA ATTCCTGATA TAGTTTTATG GCCAACATGC CATGATGATG 
     1681 TAGTTAAGAT TGTGAATCTA GCTTGCAAAT ATAATCTTTG TATCATACCA ATTGGTGGAG 
     1741 GAACAAGTGT TTCATATGGC CTGATGTGTC CTGCAGATGA GACAAGAACA ATTATTTCTT 
     1801 TGGACACTTC ACAAATGAAT CGAATTCTCT GGGTTGATGA GAACAATTTG ACAGCTCATG 
     1861 TAGAGGCTGG CATAACAGGA CAAGAGTTGG AAAGACAGCT TAAAGAAAGT GGTTATTGTA 
     1921 CAGGTCATGA ACCAGATTCC CTGGAGTTCA GTACTGTAGG AGGATGGGTA TCTACTCGCG 
     1981 CATCAGGCAT GAAGAAGAAT ATCTATGGCA ATATCGAGGA CCTGGTGGTT CATATAAAAA 
     2041 TGGTAACACC TAGAGGTATA ATAGAAAAAA GCTGTCAAGG ACCTCGTATG TCAACAGGCC 
     2101 CTGATATCCA TCACTTCATC ATGGGATCTG AAGGAACTCT TGGTGTAATA ACAGAAGCTA 
     2161 CAATAAAAAT CAGACCAGTC CCTGAATACC AAAAGTATGG CTCAGTAGCT TTCCCTAATT 
     2221 TTGAACAAGG AGTAGCCTGT TTAAGAGAAA TTGCAAAACA GAGATGTGCT CCGGCATCTA 
     2281 TTCGCCTCAT GGACAACAAG CAGTTTCAGT TTGGTCATGC TCTTAAACCT CAGGTTTCCT 
     2341 CTATTTTTAC ATCATTTTTG GACGGATTAA AAAAGTTTTA TATTACAAAG TTTAAAGGAT 
     2401 TTGACCCAAA TCAGCTAAGT GTAGCCACAT TACTGTTTGA GGGGGATCGT GAGAAGGTTC 
     2461 TTCAACATGA AAAACAAGTG TATGATATTG CTGCAAAATT TGGTGGGTTG GCAGCTGGAG 
     2521 AAGATAATGG ACAGAGAGGT TATTTGCTGA CCTATGTTAT TGCATACATT CGAGACTTGG 
     2581 CTTTGGAATA CTATGTATTA GGAGAATCTT TTGAGACTTC TGCTCCTTGG GACAGGGTGG 
     2641 TAGATCTCTG TAGAAATGTA AAAGAAAGAA TAACAAGGGA ATGCAAAGAG AAGGGTGTTC 
     2701 AGTTTGCTCC TTTTTCTACA TGCAGGGTGA CGCAGACTTA CGATGCAGGT GCTTGTATCT 
     2761 ACTTCTATTT TGCCTTTAAC TACAGGGGAA TTAGTGACCC ACTGACCGTA TTTGAACAAA 
     2821 CTGAGGCAGC TGCTAGAGAA GAAATCCTTG CTAATGGAGG GAGCCTGTCA CATCACCATG 
     2881 GAGTGGGCAA GTTACGGAAG CAATGGCTAA AGGAAAGTAT CTCTGATGTC GGCTTTGGGA 
     2941 TGCTGAAGTC TGTCAAGGAA TATGTGGACC CCAATAACAT CTTTGGAAAC AGAAACCTTT 
     3001 TAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3061 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3121 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3181 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3241 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3301 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3361 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     3421 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     3481 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     3541 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     3601 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     3661 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     3721 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     3781 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     3841 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     3901 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     3961 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4021 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4081 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4141 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4201 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4261 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     4321 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     4381 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     4441 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     4501 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     4561 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     4621 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     4681 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     4741 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     4801 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     4861 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     4921 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     4981 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5041 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5101 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5161 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5221 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     5281 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     5341 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     5401 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     5461 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     5521 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     5581 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     5641 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     5701 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     5761 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     5821 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     5881 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     5941 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6001 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6061 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6121 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6181 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6241 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     6301 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     6361 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     6421 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     6481 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     6541 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     6601 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     6661 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     6721 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     6781 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     6841 TTTAACGCGA ATTTT
//