LOCUS       RC220894                9468 bp    DNA     circular  SYN 
DEFINITION  SLIT2 (Myc-DDK-tagged)-Human slit homolog 2 (Drosophila) (SLIT2)
ACCESSION   NM_004787
VERSION     NM_004787.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5625..5716 
                     /label="Myc-DDK"  
     misc_feature    5746..6331 
                     /label="PolyA signal"  
     misc_feature    5803..5822 
                     /label="Restriction Enzyme"  
     misc_feature    6607..7279 
                     /label="ColE1"  
     misc_feature    7709..8503 
                     /label="Kan/Neo"  
     misc_feature    8538..8840 
                     /label="SV40 ori"  
     misc_feature    8926..9403 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5607..5613 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5607 
                     /label="SLIT2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGCGGCGTT GGCTGGCAGA TGCTGTCCCT GTCGCTGGGG TTAGTGCTGG 
     1081 CGATCCTGAA CAAGGTGGCA CCGCAGGCGT GCCCGGCGCA GTGCTCTTGC TCGGGCAGCA 
     1141 CAGTGGACTG TCACGGGCTG GCGCTGCGCA GCGTGCCCAG GAATATCCCC CGCAACACCG 
     1201 AGAGACTGGA TTTAAATGGA AATAACATCA CAAGAATTAC GAAGACAGAT TTTGCTGGTC 
     1261 TTAGACATCT AAGAGTTCTT CAGCTTATGG AGAATAAGAT TAGCACCATT GAAAGAGGAG 
     1321 CATTCCAGGA TCTTAAAGAA CTAGAGAGAC TGCGTTTAAA CAGAAATCAC CTTCAGCTGT 
     1381 TTCCTGAGTT GCTGTTTCTT GGGACTGCGA AGCTATACAG GCTTGATCTC AGTGAAAACC 
     1441 AAATTCAGGC AATCCCAAGG AAAGCTTTCC GTGGGGCAGT TGACATAAAA AATTTGCAAC 
     1501 TGGATTACAA CCAGATCAGC TGTATTGAAG ATGGGGCATT CAGGGCTCTC CGGGACCTGG 
     1561 AAGTGCTCAC TCTCAACAAT AACAACATTA CTAGACTTTC TGTGGCAAGT TTCAACCATA 
     1621 TGCCTAAACT TAGGACTTTT CGACTGCATT CAAACAACCT GTATTGTGAC TGCCACCTGG 
     1681 CCTGGCTCTC CGACTGGCTT CGCCAAAGGC CTCGGGTTGG TCTGTACACT CAGTGTATGG 
     1741 GCCCCTCCCA CCTGAGAGGC CATAATGTAG CCGAGGTTCA AAAACGAGAA TTTGTCTGCA 
     1801 GTGGTCACCA GTCATTTATG GCTCCTTCTT GTAGTGTTTT GCACTGCCCT GCCGCCTGTA 
     1861 CCTGTAGCAA CAATATCGTA GACTGTCGTG GGAAAGGTCT CACTGAGATC CCCACAAATC 
     1921 TTCCAGAGAC CATCACAGAA ATACGTTTGG AACAGAACAC AATCAAAGTC ATCCCTCCTG 
     1981 GAGCTTTCTC ACCATATAAA AAGCTTAGAC GAATTGACCT GAGCAATAAT CAGATCTCTG 
     2041 AACTTGCACC AGATGCTTTC CAAGGACTAC GCTCTCTGAA TTCACTTGTC CTCTATGGAA 
     2101 ATAAAATCAC AGAACTCCCC AAAAGTTTAT TTGAAGGACT GTTTTCCTTA CAGCTCCTAT 
     2161 TATTGAATGC CAACAAGATA AACTGCCTTC GGGTAGATGC TTTTCAGGAT CTCCACAACT 
     2221 TGAACCTTCT CTCCCTATAT GACAACAAGC TTCAGACCAT CGCCAAGGGG ACCTTTTCAC 
     2281 CTCTTCGGGC CATTCAAACT ATGCATTTGG CCCAGAACCC CTTTATTTGT GACTGCCATC 
     2341 TCAAGTGGCT AGCGGATTAT CTCCATACCA ACCCGATTGA GACCAGTGGT GCCCGTTGCA 
     2401 CCAGCCCCCG CCGCCTGGCA AACAAAAGAA TTGGACAGAT CAAAAGCAAG AAATTCCGTT 
     2461 GTTCAGCTAA AGAACAGTAT TTCATTCCAG GTACAGAAGA TTATCGATCA AAATTAAGTG 
     2521 GAGACTGCTT TGCGGATCTG GCTTGCCCTG AAAAGTGTCG CTGTGAAGGA ACCACAGTAG 
     2581 ATTGCTCTAA TCAAAAGCTC AACAAAATCC CGGAGCACAT TCCCCAGTAC ACTGCAGAGT 
     2641 TGCGTCTCAA TAATAATGAA TTTACCGTGT TGGAAGCCAC AGGAATCTTT AAGAAACTTC 
     2701 CTCAATTACG TAAAATAAAC TTTAGCAACA ATAAGATCAC AGATATTGAG GAGGGAGCAT 
     2761 TTGAAGGAGC ATCTGGTGTA AATGAAATAC TTCTTACGAG TAATCGTTTG GAAAATGTGC 
     2821 AGCATAAGAT GTTCAAGGGA TTGGAAAGCC TCAAAACTTT GATGTTGAGA AGCAATCGAA 
     2881 TAACCTGTGT GGGGAATGAC AGTTTCATAG GACTCAGTTC TGTGCGTTTG CTTTCTTTGT 
     2941 ATGATAATCA AATTACTACA GTTGCACCAG GGGCATTTGA TACTCTCCAT TCTTTATCTA 
     3001 CTCTAAACCT CTTGGCCAAT CCTTTTAACT GTAACTGCTA CCTGGCTTGG TTGGGAGAGT 
     3061 GGCTGAGAAA GAAGAGAATT GTCACGGGAA ATCCTAGATG TCAAAAACCA TACTTCCTGA 
     3121 AAGAAATACC CATCCAGGAT GTGGCCATTC AGGACTTCAC TTGTGATGAC GGAAATGATG 
     3181 ACAATAGTTG CTCCCCACTT TCTCGCTGTC CTACTGAATG TACTTGCTTG GATACAGTCG 
     3241 TCCGATGTAG CAACAAGGGT TTGAAGGTCT TGCCGAAAGG TATTCCAAGA GATGTCACAG 
     3301 AGTTGTATCT GGATGGAAAC CAATTTACAC TGGTTCCCAA GGAACTCTCC AACTACAAAC 
     3361 ATTTAACACT TATAGACTTA AGTAACAACA GAATAAGCAC GCTTTCTAAT CAGAGCTTCA 
     3421 GCAACATGAC CCAGCTCCTC ACCTTAATTC TTAGTTACAA CCGTCTGAGA TGTATTCCTC 
     3481 CTCGCACCTT TGATGGATTA AAGTCTCTTC GATTACTTTC TCTACATGGA AATGACATTT 
     3541 CTGTTGTGCC TGAAGGTGCT TTCAATGATC TTTCTGCATT ATCACATCTA GCAATTGGAG 
     3601 CCAACCCTCT TTACTGTGAT TGTAACATGC AGTGGTTATC CGACTGGGTG AAGTCGGAAT 
     3661 ATAAGGAGCC TGGAATTGCT CGTTGTGCTG GTCCTGGAGA AATGGCAGAT AAACTTTTAC 
     3721 TCACAACTCC CTCCAAAAAA TTTACCTGTC AAGGTCCTGT GGATGTCAAT ATTCTAGCTA 
     3781 AGTGTAACCC CTGCCTATCA AATCCGTGTA AAAATGATGG CACATGTAAT AGTGATCCAG 
     3841 TTGACTTTTA CCGATGCACC TGTCCATATG GTTTCAAGGG GCAGGACTGT GATGTCCCAA 
     3901 TTCATGCCTG CATCAGTAAC CCATGTAAAC ATGGAGGAAC TTGCCACTTA AAGGAAGGAG 
     3961 AAGAAGATGG ATTCTGGTGT ATTTGTGCTG ATGGATTTGA AGGAGAAAAT TGTGAAGTCA 
     4021 ACGTTGATGA TTGTGAAGAT AATGACTGTG AAAATAATTC TACATGTGTC GATGGCATTA 
     4081 ATAACTACAC ATGCCTTTGC CCACCTGAGT ATACAGGTGA GTTGTGTGAG GTGAAGCTGG 
     4141 ACTTCTGTGC CCAGGACCTG AACCCCTGCC AGCACGATTC AAAGTGCATC CTAACTCCAA 
     4201 AGGGATTCAA ATGTGACTGC ACACCAGGGT ACGTAGGTGA ACACTGCGAC ATCGATTTTG 
     4261 ACGACTGCCA AGACAACAAG TGTAAAAACG GAGCCCACTG CACAGATGCA GTGAACGGCT 
     4321 ATACGTGCAT ATGCCCCGAA GGTTACAGTG GCTTGTTCTG TGAGTTTTCT CCACCCATGG 
     4381 TCCTCCCTCG TACCAGCCCC TGTGATAATT TTGATTGTCA GAATGGAGCT CAGTGTATCG 
     4441 TCAGAATAAA TGAGCCAATA TGTCAGTGTT TGCCTGGCTA TCAGGGAGAA AAGTGTGAAA 
     4501 AATTGGTTAG TGTGAATTTT ATAAACAAAG AGTCTTATCT TCAGATTCCT TCAGCCAAGG 
     4561 TTCGGCCTCA GACGAACATA ACACTTCAGA TTGCCACAGA TGAAGACAGC GGAATCCTCC 
     4621 TGTATAAGGG TGACAAAGAC CATATCGCGG TAGAACTCTA TCGGGGGCGT GTTCGTGCCA 
     4681 GCTATGACAC CGGCTCTCAT CCAGCTTCTG CCATTTACAG TGTGGAGACA ATCAATGATG 
     4741 GAAACTTCCA CATTGTGGAA CTACTTGCCT TGGATCAGAG TCTCTCTTTG TCCGTGGATG 
     4801 GTGGGAACCC CAAAATCATC ACTAACTTGT CAAAGCAGTC CACTCTGAAT TTTGACTCTC 
     4861 CACTCTATGT AGGAGGCATG CCAGGGAAGA GTAACGTGGC ATCTCTGCGC CAGGCCCCTG 
     4921 GGCAGAACGG AACCAGCTTC CACGGCTGCA TCCGGAACCT TTACATCAAC AGTGAGCTGC 
     4981 AGGACTTCCA GAAGGTGCCG ATGCAAACAG GCATTTTGCC TGGCTGTGAG CCATGCCACA 
     5041 AGAAGGTGTG TGCCCATGGC ACATGCCAGC CCAGCAGCCA GGCAGGCTTC ACCTGCGAGT 
     5101 GCCAGGAAGG ATGGATGGGG CCCCTCTGTG ACCAACGGAC CAATGACCCT TGCCTTGGAA 
     5161 ATAAATGCGT ACATGGCACC TGCTTGCCCA TCAATGCGTT CTCCTACAGC TGTAAGTGCT 
     5221 TGGAGGGCCA TGGAGGTGTC CTCTGTGATG AAGAGGAGGA TCTGTTTAAC CCATGCCAGG 
     5281 CGATCAAGTG CAAGCATGGG AAGTGCAGGC TTTCAGGTCT GGGGCAGCCC TACTGTGAAT 
     5341 GCAGCAGTGG ATACACGGGG GACAGCTGTG ATCGAGAAAT CTCTTGTCGA GGGGAAAGGA 
     5401 TAAGAGATTA TTACCAAAAG CAGCAGGGCT ATGCTGCTTG CCAAACAACC AAGAAGGTGT 
     5461 CCCGATTAGA GTGCAGAGGT GGGTGTGCAG GAGGGCAGTG CTGTGGACCG CTGAGGAGCA 
     5521 AGCGGCGGAA ATACTCTTTC GAATGCACTG ACGGCTCCTC CTTTGTGGAC GAGGTTGAGA 
     5581 AAGTGGTGAA GTGCGGCTGT ACGAGGTGTG TGTCCACGCG TACGCGGCCG CTCGAGCAGA 
     5641 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5701 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5761 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5821 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5881 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5941 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     6001 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     6061 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     6121 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     6181 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     6241 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     6301 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     6361 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     6421 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     6481 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     6541 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     6601 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6661 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6721 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6781 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6841 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6901 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6961 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     7021 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     7081 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     7141 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     7201 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     7261 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     7321 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     7381 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     7441 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     7501 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     7561 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     7621 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7681 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7741 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7801 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7861 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7921 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7981 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     8041 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     8101 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     8161 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     8221 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     8281 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     8341 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     8401 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     8461 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     8521 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     8581 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     8641 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8701 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8761 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8821 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8881 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8941 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     9001 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     9061 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     9121 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     9181 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     9241 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     9301 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     9361 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     9421 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//