LOCUS       RC220859                8172 bp    DNA     circular  SYN 
DEFINITION  TACC2 (Myc-DDK-tagged)-Human transforming, acidic coiled-coil containing protein 2 (TACC2), transcript variant 2
ACCESSION   NM_206861
VERSION     NM_206861.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4327..4418 
                     /label="Myc-DDK"  
     misc_feature    4448..5033 
                     /label="PolyA signal"  
     misc_feature    4505..4524 
                     /label="Restriction Enzyme"  
     misc_feature    5309..5981 
                     /label="ColE1"  
     misc_feature    6411..7205 
                     /label="Kan/Neo"  
     misc_feature    7240..7542 
                     /label="SV40 ori"  
     misc_feature    7628..8105 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4302..4309 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4302 
                     /label="TACC2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAATGAG AACAGCACCT CGGACAACCA GAGGACTTTA TCAGCTCAGA 
     1081 CTCCAAGGTC CGCGCAGCCA CCCGGGAACA GTCAGAATAT AAAAAGGAAG CAGCAGGACA 
     1141 CGCCCGGAAG CCCTGACCAC AGAGACGCGT CCAGTTCACC TGTGGCAGAT GATATCATCC 
     1201 AGCCCGCTGC CCCCGCAGAC CTGGAAAGCC CAACCTTAGC TGCCTCTTCC TACCACGGTG 
     1261 ATGTTGTTGG CCAGGTCTCT ACGGATCTGA TAGCCCAGAG GAGTTCCGAT TCTGAAGAGG 
     1321 CATTTGAGAC CCCGGAGTCA ACGACCCCTG TCAAAGCTCC GCCAGCTCCA CCCCCACCAC 
     1381 CCCCCGAAGT CATCCCAGAA CCCGAGGTCA GCACACAGCC ACCCCCGGAA GAACCAGGAT 
     1441 GTGGTTCTGA GACAGTCCCT GTCCCTGATG GCCCACGGAG CGACTCGGTG GAAGGAAGTC 
     1501 CCTTCCGTCC CCCGTCACAC TCCTTCTCTG CCGTCTTCGA TGAAGACAAG CCGATAGCCA 
     1561 GCAGTGGGAC TTACAACTTG GACTTTGACA ACATTGAGCT TGTGGATACC TTTCAGACCT 
     1621 TGGAGCCTCG TGCCTCAGAC GCTAAGAATC AGGAGGGCAA AGTGAACACA CGGAGGAAGT 
     1681 CCACGGATTC CGTCCCCATC TCTAAGTCTA CACTGTCCCG GTCGCTCAGC CTGCAAGCCA 
     1741 GTGACTTTGA TGGTGCTTCT TCCTCAGGCA ATCCCGAGGC CGTGGCCCTT GCCCCAGATG 
     1801 CATATAGCAC GGGTTCCAGC AGTGCTTCTA GTACCCTTAA GCGAACTAAA AAACCGAGGC 
     1861 CGCCTTCCTT AAAAAAGAAA CAGACCACCA AGAAACCCAC AGAGACCCCC CCAGTGAAGG 
     1921 AGACGCAACA GGAGCCAGAT GAAGAGAGCC TTGTCCCCAG TGGGGAGAAT CTAGCATCTG 
     1981 AGACGAAAAC GGAATCTGCC AAGACGGAAG GTCCTAGCCC AGCCTTATTG GAGGAGACGC 
     2041 CCCTTGAGCC CGCTGTGGGG CCCAAAGCTG CCTGCCCTCT GGACTCAGAG AGTGCAGAAG 
     2101 GGGTTGTCCC CCCGGCTTCT GGAGGTGGCA GAGTGCAGAA CTCACCCCCT GTCGGGAGGA 
     2161 AAACGCTGCC TCTTACCACG GCCCCGGAGG CAGGGGAGGT AACCCCATCG GATAGCGGGG 
     2221 GGCAAGAGGA CTCTCCAGCC AAAGGGCTCT CCGTAAGGCT GGAGTTTGAC TATTCTGAGG 
     2281 ACAAGAGTAG TTGGGACAAC CAGCAGGAAA ACCCCCCTCC TACCAAAAAG ATAGGCAAAA 
     2341 AGCCAGTTGC CAAAATGCCC CTGAGGAGGC CAAAGATGAA AAAGACACCC GAGAAACTTG 
     2401 ACAACACTCC TGCCTCACCT CCCAGATCCC CTGCTGAACC CAATGACATC CCCATTGCTA 
     2461 AAGGTACTTA CACCTTTGAT ATTGACAAGT GGGATGACCC CAATTTTAAC CCTTTTTCTT 
     2521 CCACCTCAAA AATGCAGGAG TCTCCCAAAC TGCCCCAACA ATCATACAAC TTTGACCCAG 
     2581 ACACCTGTGA TGAGTCCGTT GACCCCTTTA AGACATCCTC TAAGACCCCC AGCTCACCTT 
     2641 CTAAATCCCC AGCCTCCTTT GAGATCCCAG CCAGTGCTAT GGAAGCCAAT GGAGTGGACG 
     2701 GGGATGGGCT AAACAAGCCC GCCAAGAAGA AGAAGACGCC CCTAAAGACT GACACATTTA 
     2761 GGGTGAAAAA GTCGCCAAAA CGGTCTCCTC TCTCTGATCC ACCTTCCCAG GACCCCACCC 
     2821 CAGCTGCTAC ACCAGAAACA CCACCAGTGA TCTCTGCGGT GGTCCACGCC ACAGATGAGG 
     2881 AAAAGCTGGC GGTCACCAAC CAGAAGTGGA CGTGCATGAC AGTGGACCTA GAGGCTGACA 
     2941 AACAGGACTA CCCGCAGCCC TCGGACCTGT CCACCTTTGT AAACGAGACC AAATTCAGTT 
     3001 CACCCACTGA GGAGTTGGAT TACAGAAACT CCTATGAAAT TGAATATATG GAGAAAATTG 
     3061 GCTCCTCCTT ACCTCAGGAC GACGATGCCC CGAAGAAGCA GGCCTTGTAC CTTATGTTTG 
     3121 ACACTTCTCA GGAGAGCCCT GTCAAGTCAT CTCCCGTCCG CATGTCAGAG TCCCCGACGC 
     3181 CGTGTTCAGG GTCAAGTTTT GAAGAGACTG AAGCCCTTGT GAACACTGCT GCGAAAAACC 
     3241 AGCATCCTGT CCCACGAGGA CTGGCCCCTA ACCAAGAGTC ACACTTGCAG GTGCCAGAGA 
     3301 AATCCTCCCA GAAGGAGCTG GAGGCCATGG GCTTGGGCAC CCCTTCAGAA GCGATTGAAA 
     3361 TTACAGCTCC CGAGGGCTCC TTTGCCTCTG CTGACGCCCT CCTCAGCAGG CTAGCTCACC 
     3421 CCGTCTCTCT CTGTGGTGCA CTTGACTATC TGGAGCCCGA CTTAGCAGAA AAGAACCCCC 
     3481 CACTATTCGC TCAGAAACTC CAGGAGGAGT TAGAGTTTGC CATCATGCGG ATAGAAGCCC 
     3541 TGAAGCTGGC CAGGCAGATT GCTTTGGCTT CCCGCAGCCA CCAGGATGCC AAGAGAGAGG 
     3601 CTGCTCACCC AACAGACGTC TCCATCTCCA AAACAGCCTT GTACTCCCGC ATCGGGACCG 
     3661 CTGAGGTGGA GAAACCTGCA GGCCTTCTGT TCCAGCAGCC CGACCTGGAC TCTGCCCTCC 
     3721 AGATCGCCAG AGCAGAGATC ATAACCAAGG AGAGAGAGGT CTCAGAATGG AAAGATAAAT 
     3781 ATGAAGAAAG CAGGCGGGAA GTGATGGAAA TGAGGAAAAT AGTGGCCGAG TATGAGAAGA 
     3841 CCATCGCTCA GATGATAGAG GACGAACAGA GAGAGAAGTC AGTCTCCCAC CAGACGGTGC 
     3901 AGCAGCTGGT TCTGGAGAAG GAGCAAGCCC TGGCCGACCT GAACTCCGTG GAGAAGTCTC 
     3961 TGGCCGACCT CTTCAGAAGA TATGAGAAGA TGAAGGAGGT CCTAGAAGGC TTCCGCAAGA 
     4021 ATGAAGAGGT GTTGAAGAGA TGTGCGCAGG AGTACCTGTC CCGGGTGAAG AAGGAGGAGC 
     4081 AGAGGTACCA GGCCCTGAAG GTGCACGCGG AGGAGAAACT GGACAGGGCC AATGCTGAGA 
     4141 TTGCTCAGGT TCGAGGCAAG GCCCAGCAGG AGCAAGCCGC CCACCAGGCC AGCCTGCGGA 
     4201 AGGAGCAGCT GCGAGTGGAC GCCCTGGAAA GGACGCTGGA GCAGAAGAAT AAAGAAATAG 
     4261 AAGAACTCAC CAAGATTTGT GACGAACTGA TTGCCAAAAT GGGGAAAAGC AGCGGACCGA 
     4321 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4381 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4441 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4501 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4561 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4621 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4681 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4741 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4801 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4861 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4921 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4981 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5041 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5101 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5161 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5221 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5281 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5341 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5401 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5461 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5521 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5581 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5641 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5701 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5761 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5821 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5881 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5941 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6001 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6061 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6121 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6181 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6241 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6301 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6361 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6421 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6481 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6541 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6601 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6661 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6721 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6781 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6841 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6901 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6961 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7021 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7081 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7141 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7201 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7261 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7321 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7381 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7441 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7501 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7561 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7621 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7681 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7741 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7801 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7861 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7921 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7981 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8041 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8101 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8161 AACGCGAATT TT
//