LOCUS       RC220846                8160 bp    DNA     circular  SYN 
DEFINITION  PHKB (Myc-DDK-tagged)-Human phosphorylase kinase, beta (PHKB), transcript variant 1
ACCESSION   NM_000293
VERSION     NM_000293.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4317..4408 
                     /label="Myc-DDK"  
     misc_feature    4438..5023 
                     /label="PolyA signal"  
     misc_feature    4495..4514 
                     /label="Restriction Enzyme"  
     misc_feature    5299..5971 
                     /label="ColE1"  
     misc_feature    6401..7195 
                     /label="Kan/Neo"  
     misc_feature    7230..7532 
                     /label="SV40 ori"  
     misc_feature    7618..8095 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4299..4305 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4299 
                     /label="PHKB"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGGGCG GCGGGACTCA CGGCAGAAGT GAGCTGGAAG GTCTTGGAGC 
     1081 GAAGAGCTCG GACCAAGCGC TCAGGCTCAG TTTATGAACC TCTTAAAAGC ATTAATCTTC 
     1141 CAAGACCTGA TAATGAAACT CTCTGGGATA AGTTGGACCA TTATTACAGA ATTGTCAAGT 
     1201 CAACATTGCT GCTGTATCAA AGTCCAACTA CCGGTCTCTT TCCCACTAAA ACATGCGGTG 
     1261 GTGACCAGAA GGCCAAGATC CAGGACAGCC TATACTGCGC TGCTGGGGCC TGGGCTTTGG 
     1321 CTCTTGCATA CAGGCGAATT GATGATGACA AGGGAAGGAC CCATGAGCTG GAGCACTCAG 
     1381 CTATAAAATG CATGAGAGGA ATTCTCTACT GCTATATGCG TCAGGCCGAT AAGGTCCAGC 
     1441 AGTTTAAGCA GGATCCACGC CCAACAACAT GTCTTCACTC TGTTTTCAAT GTGCATACAG 
     1501 GAGATGAGTT GCTTTCCTAT GAGGAATATG GTCATCTTCA GATAAATGCA GTGTCACTTT 
     1561 ATCTCCTTTA CCTTGTGGAA ATGATTTCCT CAGGACTCCA GATTATCTAC AACACTGATG 
     1621 AGGTCTCTTT TATTCAAAAC CTTGTATTTT GTGTGGAAAG AGTTTACCGT GTGCCTGACT 
     1681 TTGGTGTCTG GGAAAGAGGA AGCAAATATA ATAATGGCAG CACAGAGCTA CATTCGAGCT 
     1741 CGGTTGGTTT AGCAAAAGCA GCTCTAGAAG CAATTAATGG ATTCAACCTT TTTGGCAACC 
     1801 AGGGCTGTTC GTGGTCAGTT ATATTTGTGG ATCTCGATGC TCACAATCGC AACAGGCAAA 
     1861 CTTTGTGCTC GCTGTTACCC AGAGAATCAA GATCACATAA TACAGATGCT GCCCTGCTCC 
     1921 CCTGCATCAG TTATCCTGCA TTTGCCCTGG ATGATGAAGT TCTTTTTAGC CAGACACTTG 
     1981 ATAAAGTGGT TAGAAAATTA AAAGGAAAAT ATGGATTTAA ACGTTTCTTG AGAGATGGGT 
     2041 ATAGAACATC ATTGGAAGAT CCCAACAGAT GCTACTACAA GCCAGCTGAA ATTAAGCTAT 
     2101 TTGATGGCAT TGAATGTGAA TTTCCCATAT TTTTCCTTTA TATGATGATT GATGGAGTTT 
     2161 TTAGAGGCAA TCCTAAGCAA GTACAGGAAT ATCAGGATCT TTTGACTCCA GTACTTCATC 
     2221 ATACCACAGA AGGATATCCT GTTGTACCAA AGTACTATTA TGTGCCAGCT GACTTTGTAG 
     2281 AATATGAAAA AAATAACCCT GGTAGTCAAA AACGATTTCC TAGCAACTGT GGCCGTGATG 
     2341 GAAAACTGTT TCTTTGGGGA CAAGCACTTT ATATCATCGC AAAACTCCTG GCTGATGAAC 
     2401 TTATTAGTCC TAAAGACATT GATCCTGTCC AGCGCTATGT CCCACTAAAG GATCAACGTA 
     2461 ACGTGAGCAT GAGGTTTTCC AATCAGGGCC CACTGGAAAA TGACTTGGTA GTTCATGTGG 
     2521 CACTTATAGC AGAAAGCCAA AGACTTCAAG TTTTTCTGAA CACATATGGT ATTCAAACTC 
     2581 AAACTCCTCA ACAAGTAGAA CCCATTCAGA TATGGCCTCA GCAGGAGCTT GTGAAAGCTT 
     2641 ATTTGCAGCT GGGTATCAAT GAAAAGTTAG GACTCTCTGG AAGGCCAGAC AGGCCCATTG 
     2701 GCTGCCTCGG GACATCAAAG ATTTATCGCA TTCTAGGAAA GACTGTGGTT TGTTACCCGA 
     2761 TTATTTTCGA CCTAAGTGAT TTCTACATGT CTCAGGATGT TTTCCTGCTG ATAGATGACA 
     2821 TAAAGAATGC GCTGCAGTTC ATTAAACAAT ATTGGAAAAT GCATGGACGT CCACTTTTCC 
     2881 TTGTTCTCAT CCGGGAAGAC AATATAAGAG GTAGCCGGTT CAACCCCATA TTAGATATGC 
     2941 TGGCAGCCCT TAAAAAAGGA ATAATTGGAG GAGTCAAAGT TCATGTGGAT CGTCTACAGA 
     3001 CACTAATATC TGGAGCTGTG GTAGAACAAC TTGATTTCCT ACGAATCAGT GACACAGAAG 
     3061 AGCTTCCAGA ATTTAAGAGT TTTGAGGAAC TAGAACCTCC CAAACATTCA AAAGTCAAAC 
     3121 GGCAAAGCAG CACCCCTAGT GCTCCTGAAC TGGGACAGCA GCCGGATGTC AACATTAGTG 
     3181 AATGGAAGGA CAAACCCACC CACGAAATTC TTCAAAAACT GAATGATTGC AGTTGTCTGG 
     3241 CTAGCCAAGC CATCCTGCTG GGTATACTGC TCAAAAGAGA AGGCCCCAAC TTCATCACAA 
     3301 AGGAAGGTAC CGTTTCTGAT CACATTGAGA GAGTCTATAG AAGAGCTGGC AGCCAAAAAC 
     3361 TTTGGTTGGC GGTGCGCTAC GGGGCTGCAT TTACCCAGAA ATTTTCTTCC TCTATAGCCC 
     3421 CACACATTAC TACTTTTCTG GTACATGGGA AACAGGTAAC TCTGGGTGCC TTTGGGCATG 
     3481 AAGAAGAAGT TATCTCTAAT CCTTTGTCTC CAAGAGTGAT TCAAAACATC ATCTATTATA 
     3541 AGTGTAACAC CCATGATGAG AGGGAAGCGG TCATTCAGCA AGAACTGGTC ATCCATATTG 
     3601 GCTGGATCAT CTCCAATAAC CCTGAGTTAT TCAGTGGCAT GCTGAAAATA CGAATCGGGT 
     3661 GGATCATCCA TGCCATGGAG TATGAACTTC AGATCCGTGG CGGAGACAAG CCAGCCTTGG 
     3721 ACTTGTATCA GCTGTCACCT AGTGAAGTTA AACAGCTTCT GCTGGATATT CTGCAGCCTC 
     3781 AACAGAATGG AAGATGTTGG CTGAACAGGC GTCAGATCGA TGGGTCTTTG AATAGAACTC 
     3841 CCACCGGGTT CTATGACCGA GTGTGGCAGA TTCTGGAGCG CACGCCCAAT GGGATCATTG 
     3901 TTGCTGGGAA GCATTTGCCT CAGCAACCAA CCCTGTCAGA TATGACCATG TATGAGATGA 
     3961 ATTTCTCTCT CCTTGTTGAA GACACGTTGG GAAATATTGA CCAGCCACAG TACAGACAGA 
     4021 TCGTTGTAGA GTTACTTATG GTTGTATCCA TTGTACTGGA AAGAAACCCC GAGCTAGAAT 
     4081 TTCAAGACAA AGTAGATCTA GACAGACTGG TCAAAGAAGC ATTTAATGAA TTTCAAAAAG 
     4141 ATCAGAGTCG GCTAAAGGAA ATTGAAAAAC AAGATGACAT GACTTCCTTT TACAACACTC 
     4201 CTCCCCTGGG AAAAAGAGGA ACATGCAGCT ATTTGACAAA GGCGGTGATG AATCTGCTGC 
     4261 TGGAAGGAGA AGTCAAGCCA AACAATGATG ACCCGTGTCT GATTAGCACG CGTACGCGGC 
     4321 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4381 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4441 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4501 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4561 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4621 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4681 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4741 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4801 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4861 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4921 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4981 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5041 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5101 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5161 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5221 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5281 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5341 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5401 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5461 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5521 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5581 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5641 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5701 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5761 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5821 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5881 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5941 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6001 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6061 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6121 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6181 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6241 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6301 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6361 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6421 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6481 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6541 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6601 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6661 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6721 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6781 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6841 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6901 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6961 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7021 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7081 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7141 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7201 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7261 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7321 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7381 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7441 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7501 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7561 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7621 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7681 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7741 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7801 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7861 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7921 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7981 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8041 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8101 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//