LOCUS       RC220826                8079 bp    DNA     circular  SYN 
DEFINITION  AGBL1 (Myc-DDK-tagged)-Human ATP/GTP binding protein-like 1 (AGBL1)
ACCESSION   NM_152336
VERSION     NM_152336.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4236..4327 
                     /label="Myc-DDK"  
     misc_feature    4357..4942 
                     /label="PolyA signal"  
     misc_feature    4414..4433 
                     /label="Restriction Enzyme"  
     misc_feature    5218..5890 
                     /label="ColE1"  
     misc_feature    6320..7114 
                     /label="Kan/Neo"  
     misc_feature    7149..7451 
                     /label="SV40 ori"  
     misc_feature    7537..8014 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4218..4224 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4218 
                     /label="AGBL1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATCAGCAAG GGTGGCAGTG AAGCTCTTCT GCAGACCCTG GTAGACACAG 
     1081 CGAGGACAGC TCCTCCAGAC TATGACATCC TCCTGCCTCT CTTCCGGCTG CTGGCCAAAG 
     1141 TTGGCCTAAG AGATAAAAAG ATTGGACGGA AGGCCCTAGA ATTGGAAGCA CTTGATGTGA 
     1201 CATTGATTCT GGCCAGGAAG AACCTATCCC ATGGCCAGAA TCTCCTCCAC TGTCTCTGGG 
     1261 CTCTGCGTGT GTTTGCCTCC AGTGTCTCCA TGGGAGCCAT GCTGGGAATT AATGGAGCCA 
     1321 TGGAACTGCT TTTCAAGGTT ATTACTCCTT ACACCCGAAA GCGCACCCAA GCAATCAGGG 
     1381 CAGCCACTGA AGTTTTGGCA GCATTGCTGA AATCCAAGTC GAACGGCCGC AGAGCAGTGA 
     1441 ACCGAGGCTA CGTCACCAGC CTGCTCGGGC TGCACCAGGA CTGGCACAGC CATGACACAG 
     1501 CCAACGCCTA CGTGCAGATC CGACGGGGCT TGCTGCTCTG CCTCAGGCAC ATTGCTGCCC 
     1561 TCCGGTCCGG CAGGGAGGCC TTCCTGGCAG CACAGGGCAT GGAGATCCTC TTCAGCACCA 
     1621 CACAGAACTG CCTGGATGAC AAGAGCATGG AGCCCGTCAT CTCTGTGGTG CTTCAGATCC 
     1681 TGAGGCAGTG CTACCCTACG AGTCCACTTC CCTTGGTCAC AGCCAGCAGT GCCTATGCCT 
     1741 TCCCGGTCCC CGGGTGCATC ACCACTGAAC CTCCACATGA TCTACCTGAA GAGGATTTTG 
     1801 AAGATGATGG CGATGATGAA GTGGACAAAG ACTCTGATAC TGAAGATGGG AAAGTGGAAG 
     1861 ATGATGACTT GGAAACAGAC GTGAACAAGC TGAGTTCCAA ACCTGGTCTT GACCGACCTG 
     1921 AAGAGGAACT GATGCAATAT GAGGTGATGT GTCTTGAGCT CTCCTATAGC TTTGAGGAAC 
     1981 TGCAGTCCAA ACTTGGAGAT GATTTGAACT CTGAAAAGAC TCAGTATGCC AATCACCACC 
     2041 ACATTCCAGC CGCTGCCTCC TCAAAACAGC ATTGCTACAG CAAGGACCAA AGCTCCTGTG 
     2101 GGCAAGAAAG AGAATATGCT GTCCAGACTT CCCTTCTGTG CAGGGTGAAG ACGGGAAGGT 
     2161 CCACTGTGCA TCTAGGCTCC AAAAAAAATC CTGGAGTGAA CCTGTACCAA AATGTGCAAT 
     2221 CCAATAGTCT CAGGAGAGAT TCTTCTGAAA GTGAAATCCC TGACATTCAG GCTTCCCCGA 
     2281 AAGCAGATGC CTGGGACGTA GATGCAATTT TCTGCCCAAG GATGAGTGCC TCCTTTTCTA 
     2341 ATTCCACTAG GACTAGAGAA GTTGTCAAAG TAATAGATAA GCTTCTGCAG ACACATCTGA 
     2401 AGCGTGTCCC TTTCCACGAT CCCTATCTTT ATATGGCCAA AGCCAGAAGA ACCAGCTCTG 
     2461 TGGTGGACTT CAAGATGATG GCATTTCCTG ATGTCTGGGG ACACTGTCCC CCTCCCACCA 
     2521 CCCAGCCTAT GTTGGAACGA AAATGTGGAG TCCAAAGGAT CAGGATATTT GAGGATATTC 
     2581 GGAGGCTCAT CCAGCCAAGT GATGTTATAA ATAAAGTTGT CTTCAGTTTA GATGAGCCTT 
     2641 GGCCTTTGCA AGACAATGCT TCCAATTGTT TACGGTTCTT CTCCAAATTT GAGTCAGGAA 
     2701 ATCTTCGCAA AGCCATCCAA GTGCGTGAGT TCGAGTATGA CTTGCTGGTC AACGCAGATG 
     2761 TGAATAGCAC CCAGCACCAG CAGTGGTTCT ATTTCAAAGT GAGCGGTATG CAGGCGGCCA 
     2821 TCCCTTACCA CTTCAACATC ATCAACTGTG AGAAGCCCAA CAGCCAGTTT AATTATGGGA 
     2881 TGCAGCCCAC CCTATATTCT GTGAAGGAGG CTCTTCTTGG CAAACCCACC TGGATAAGGA 
     2941 CAGGCCATGA AATATGTTAT TACAAAAATC ATTATCGCCA GAGTACAGCT GTTGCAGGCG 
     3001 GAGCATCTGG GAAGTGCTAC TATACCCTCA CCTTTGCTGT CACCTTCCCA CACAGTGAGG 
     3061 ATGTCTGCTA CCTGGCCTAC CACTATCCCT ATACCTACAC AGCCCTCATG ACTCATCTTG 
     3121 ACATCCTGGA AAAGAGTGTC AACCTCAAAG AGGTCTACTT CCGGCAAGAT GTTCTCTGCC 
     3181 AGACGCTGGG AGGGAATCCG TGTCCCTTGG TGACCATCAC GGCCATGCCT GAGTCCAACA 
     3241 GTGATGAGCA TCTAGAGCAG TTCCGACATC GTCCATATCA GGTGATCACT GCTCGAGTTC 
     3301 ATCCAGGAGA GAGCAATGCC AGTTGGGTGA TGAAGGGTAC CTTGGAGTTC CTGGTCAGCA 
     3361 GTGACCCTGT GGCTAGGCTC TTGAGGGAAA ACTTCATCTT CAAGATCATA CCCATGCTCA 
     3421 ACCCAGATGG TGTCATCAAC GGCAACCACA GATGCTCACT GAGCGGGGAA GATTTGAACA 
     3481 GACAATGGCT TTCTCCCAGT GCTCATCTGC AGCCAACCAT TTACCATGCC AAAGGCCTCC 
     3541 TCTACCACCT GAGCAGCATT GGCCGAAGTC CCGTGGTTTT CTGTGACTTC CATGGCCACT 
     3601 CCCAAAAGAA GAATGTGTTC CTTTATGGCT GTAGCATCAA GGAAACCTTG TGGCAAGCAG 
     3661 CATGCACTGT GGGCACATCT ACTATCCTAG AGGAGGTCAA CTACAGGACT CTTCCCAAAA 
     3721 TCCTTGATAA GCTAGCACCA GCATTCACAA TGAGCAGCTG CAGCTTTCTC GTGGAGAAAT 
     3781 CTCGAGCTTC CACGGCCCGG GTGGTGGTGT GGAGAGAGAT GGGGGTGTCC AGAAGCTACA 
     3841 CCATGGAAAG CAGCTACTGT GGCTGCAACC AGGGCCCTTA TCAGTGTACA CAGAGGCTGT 
     3901 TGGAGAGGAC AAAGAATGAA AGGGCTCACC CAGTGGATGG CCTTCAGGGT CTACAGTTTG 
     3961 GTACCAGAGA ACTGGAGGAG ATGGGAGCCA TGTTCTGTTT GGGCCTCCTC ATCCTGGAGC 
     4021 TCAAATCTGC CAGCTGCAGC CATCAGCTCC TGGCTCAAGC TGCAACTCTG CTGAGTGCTG 
     4081 AGGAGGACGC TCTGGACCAG CACCTCCAAC GATTAAAATC ATCCAATTTC CTGCCAAAGC 
     4141 ATATTTGGTT TGCTTACCAC TTTTTTGCCA TTACAAACTT TTTCAAGATG AACCTTCTTC 
     4201 TGCATGTCTC CCCAGTTTGT GACACTACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4261 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4321 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4381 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4441 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4501 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4561 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4621 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4681 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4741 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4801 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4861 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4921 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4981 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5041 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5101 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5161 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5221 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5281 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5341 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5401 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5461 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5521 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5581 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5641 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5701 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5761 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5821 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5881 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5941 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6001 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6061 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6121 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6181 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6241 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6301 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6361 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6421 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6481 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6541 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6601 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6661 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6721 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6781 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6841 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6901 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6961 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7021 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7081 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7141 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7201 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7261 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7321 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7381 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7441 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7501 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7561 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7621 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7681 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7741 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7801 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7861 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7921 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7981 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8041 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//