LOCUS       RC220801                8415 bp    DNA     circular  SYN 
DEFINITION  SMG7 (Myc-DDK-tagged)-Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 4
ACCESSION   NM_201569
VERSION     NM_201569.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4572..4663 
                     /label="Myc-DDK"  
     misc_feature    4693..5278 
                     /label="PolyA signal"  
     misc_feature    4750..4769 
                     /label="Restriction Enzyme"  
     misc_feature    5554..6226 
                     /label="ColE1"  
     misc_feature    6656..7450 
                     /label="Kan/Neo"  
     misc_feature    7485..7787 
                     /label="SV40 ori"  
     misc_feature    7873..8350 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4554..4560 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4554 
                     /label="SMG7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCCTGCAG AGCGCGCAGT ACCTCCGGCA GGCAGAAGTC CTGAAGGCTG 
     1081 ACATGACAGA TTCTAAGCTG GGTCCAGCTG AAGTCTGGAC ATCCAGGCAG GCTCTGCAGG 
     1141 ACCTGTACCA GAAAATGCTA GTTACCGATT TGGAATACGC TTTAGACAAG AAAGTAGAAC 
     1201 AGGATCTCTG GAATCACGCC TTTAAGAATC AGATCACAAC ACTGCAAGGC CAGGCAAAGA 
     1261 ATCGAGCAAA TCCGAATCGG AGTGAAGTTC AGGCAAACCT TTCTCTGTTC CTAGAGGCAG 
     1321 CTAGTGGCTT CTATACTCAG TTATTACAAG AACTGTGTAC AGTATTTAAT GTAGATTTAC 
     1381 CATGCCGTGT GAAGTCTTCC CAATTGGGAA TTATCAGCAA TAAACAGACG CATACCAGCG 
     1441 CCATAGTGAA GCCACAGTCT AGCTCCTGTT CCTATATCTG CCAGCACTGC CTCGTCCACC 
     1501 TTGGAGACAT TGCTCGATAC AGAAACCAGA CCAGCCAGGC AGAGTCCTAC TATAGGCATG 
     1561 CAGCTCAGCT TGTCCCCTCC AATGGTCAGC CTTATAATCA GTTGGCTATC TTAGCTTCTT 
     1621 CCAAAGGAGA CCATCTGACC ACAATTTTCT ACTACTGCAG AAGCATTGCT GTGAAGTTCC 
     1681 CTTTCCCAGC TGCCTCCACT AATCTGCAAA AAGCACTTTC TAAAGCACTG GAAAGCCGAG 
     1741 ATGAGGTGAA AACCAAGTGG GGTGTTTCTG ACTTCATCAA GGCCTTTATT AAATTCCACG 
     1801 GTCATGTGTA CCTGAGTAAG AGCTTGGAAA AGTTGAGCCC TCTTCGAGAG AAATTGGAAG 
     1861 AACAGTTTAA GAGGCTGCTA TTCCAAAAAG CTTTCAACTC TCAGCAGTTA GTTCATGTCA 
     1921 CTGTCATTAA CCTGTTTCAA CTTCATCACC TTCGTGACTT TAGCAATGAA ACCGAGCAGC 
     1981 ACACTTATAG CCAAGATGAG CAGCTATGTT GGACACAGTT GCTGGCCCTC TTTATGTCTT 
     2041 TTCTTGGCAT CCTGTGCAAG TGTCCTCTAC AGAATGAGTC TCAGGAGGAG TCCTACAATG 
     2101 CCTATCCTCT TCCAGCAGTC AAGGTCTCCA TGGACTGGCT AAGACTCAGA CCCAGGGTCT 
     2161 TTCAGGAGGC AGTGGTGGAT GAAAGACAGT ACATTTGGCC CTGGTTGATT TCTCTTCTGA 
     2221 ATAGTTTCCA TCCCCATGAA GAGGACCTCT CAAGTATTAG TGCGACACCA CTTCCAGAGG 
     2281 AGTTTGAATT ACAAGGATTT TTGGCATTGA GACCTTCTTT CAGGAACTTG GATTTTTCCA 
     2341 AAGGTCACCA GGGTATTACA GGGGACAAAG AAGGCCAGCA ACGACGAATA CGACAGCAAC 
     2401 GCTTGATCTC TATAGGCAAA TGGATTGCTG ATAATCAGCC AAGGCTGATT CAGTGTGAAA 
     2461 ATGAGGTAGG GAAATTGTTG TTTATCACAG AAATCCCAGA ATTAATACTG GAAGACCCCA 
     2521 GTGAAGCCAA AGAGAACCTC ATTCTGCAAG AAACATCTGT GATAGAGTCG CTGGCTGCAG 
     2581 ATGGGAGCCC AGGGCTAAAA TCAGTGCTAT CTACAAGCCG AAATTTAAGC AACAACTGTG 
     2641 ACACAGGAGA GAAGCCAGTG GTTACCTTCA AAGAAAACAT TAAGACACGA GAAGTGAACA 
     2701 GAGACCAAGG AAGAAGTTTT CCTCCCAAAG AGGTAAAATC CCAGACAGAA CTAAGAAAGA 
     2761 CTCCAGTGTC TGAAGCCAGA AAAACACCTG TAACTCAAAC CCCAACTCAA GCAAGTAACT 
     2821 CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC AGGCCAGGGT 
     2881 TTCCGCCCCC AACATATGTT ATCCCCCCGC CTGTGGCATT TTCTATGGGC TCAGGTTACA 
     2941 CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT CACTCTCCAG 
     3001 CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG CAACGGCCCT 
     3061 CTGGACCAGG GCCAATGAAC CAGGGACCTC AACAATCACA GCCACCTTCC CAGCAACCCC 
     3121 TTACATCTTT ACCAGCTCAG CCAACAGCAC AGTCTACAAG CCAGCTGCAG GTTCAAGCTC 
     3181 TAACTCAGCA ACAACAATCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA AGCCCGCCTC 
     3241 ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG TGGAATCCCC 
     3301 CTCAGGTTCA AGGCCCATTA GGGAAAATTA TGCCTGTGAA ACAGCCCTAC TACCTTCAGA 
     3361 CCCAAGACCC CATAAAACTG TTTGAGCCGT CATTGCAACC TCCTGTAATG CAGCAGCAGC 
     3421 CTCTAGAAAA AAAAATGAAG CCTTTTCCCA TGGAGCCATA TAACCATAAT CCCTCAGAAG 
     3481 TCAAGGTCCC AGAATTCTAC TGGGATTCTT CCTACAGCAT GGCTGATAAC AGATCTGTAA 
     3541 TGGCACAGCA AGCAAACATA GACCGCAGGG GCAAACGGTC ACCAGGAGTC TTCCGTCCAG 
     3601 AGCAGGATCC TGTACCCAGA ATGCCGTTTG AGGACCCCAA GAGCTCCCCT CTGCTTCCTC 
     3661 CGGACCTGTT AAAGAGTCTG GCTGCCTTGG AGGAAGAGGA AGAGCTGATT TTTTCTAACC 
     3721 CTCCTGATCT TTACCCGGCT CTGCTGGGGC CTCTCGCCTC TCTTCCTGGA CGAAGCCTTT 
     3781 TTAAATCCTT ATTGGAGAAG CCCTCAGAGC TCATGTCACA TTCATCCTCT TTCCTGTCCC 
     3841 TCACCGGATT CTCTCTCAAT CAGGAAAGAT ACCCAAATAA TAGTATGTTC AATGAGGTAT 
     3901 ATGGGAAAAA CCTGACATCC AGCTCCAAAG CAGAACTCAG TCCCTCAATG GCCCCCCAGG 
     3961 AAACATCTCT GTATTCCCTT TTTGAAGGGA CTCCGTGGTC TCCATCACTT CCTGCCAGTT 
     4021 CAGATCATTC AACACCAGCC AGCCAGTCTC CTCATTCCTC TAACCCAAGC AGCCTACCCA 
     4081 GCTCTCCTCC AACACACAAC CATAATTCTG TTCCATTCTC CAATTTTGGA CCCATTGGGA 
     4141 CTCCAGATAA CAGGGATAGA AGGACTGCAG ATCGGTGGAA AACTGATAAG CCAGCCATGG 
     4201 GTGGGTTTGG CATTGATTAT CTCTCAGCAA CGTCATCCTC TGAGAGCAGT TGGCATCAGG 
     4261 CCAGCACTCC GAGTGGCACC TGGACAGGCC ATGGCCCTTC CATGGAGGAT TCCTCTGCTG 
     4321 TCCTCATGGA AAGCCTAAAG AAGCAACAGC ATGGGGTCCA GCAGTTGGGG CCCAAAAGAC 
     4381 AGTCTGAAGA GGAAGGAAGC AGCAGTATCT GCGTAGCCCA CAGAGGGCCC AGGCCCCTGC 
     4441 CCAGCTGCAG TCTCCCAGCC TCCACTTTCA GAGTGAAATT CAAGGCAGCA CGGACATGTG 
     4501 CCCATCAGGC ACAGAAGAAA ACACGACGTC GTCCATTTTG GAAGAGACGA AAGAAAGGAA 
     4561 AAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4621 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4681 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4741 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4801 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4861 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4921 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4981 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5041 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5101 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5161 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5221 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5281 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5341 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5401 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5461 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5521 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5581 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5641 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5701 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5761 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5821 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5881 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5941 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6001 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6061 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6121 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6181 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6241 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6301 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6361 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6421 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6481 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6541 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6601 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6661 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6721 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6781 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6841 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6901 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6961 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7021 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7081 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7141 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7201 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7261 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7321 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7381 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7441 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7501 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7561 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7621 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7681 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7741 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7801 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7861 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7921 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7981 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8041 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8101 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8161 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8221 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8281 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8341 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8401 TTTAACGCGA ATTTT
//