LOCUS       RC220800                8091 bp    DNA     circular  SYN 
DEFINITION  DAAM2 (Myc-DDK-tagged)-Human dishevelled associated activator of morphogenesis 2 (DAAM2), transcript variant 2
ACCESSION   NM_015345
VERSION     NM_015345.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4246..4337 
                     /label="Myc-DDK"  
     misc_feature    4367..4952 
                     /label="PolyA signal"  
     misc_feature    4424..4443 
                     /label="Restriction Enzyme"  
     misc_feature    5228..5900 
                     /label="ColE1"  
     misc_feature    6330..7124 
                     /label="Kan/Neo"  
     misc_feature    7159..7461 
                     /label="SV40 ori"  
     misc_feature    7547..8024 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4221..4228 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4221 
                     /label="DAAM2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCCCCGC AAGAGGAGCC ACCATGGCCT GGGCTTCCTG TGCTGCTTCG 
     1081 GGGGCAGTGA CATCCCCGAA ATCAACCTCC GGGACAACCA CCCTCTGCAG TTCATGGAGT 
     1141 TCTCCAGCCC CATCCCGAAC GCAGAGGAGC TCAACATCCG CTTTGCAGAG CTGGTGGATG 
     1201 AATTGGATCT CACTGACAAA AACCGAGAGG CTATGTTTGC ACTGCCCCCT GAGAAGAAAT 
     1261 GGCAGATCTA CTGCAGCAAG AAGAAGGAGC AGGAGGACCC CAACAAGCTG GCAACCAGCT 
     1321 GGCCTGACTA TTACATCGAC CGCATCAATT CCATGGCTGC GATGCAGAGT CTGTACGCGT 
     1381 TTGATGAGGA GGAGACGGAG ATGAGGAACC AAGTCGTGGA AGACCTGAAG ACAGCCCTCC 
     1441 GGACACAGCC TATGAGGTTT GTGACCCGCT TCATTGAGCT GGAGGGCTTG ACCTGTCTGC 
     1501 TAAATTTCCT CCGGAGCATG GACCACGCCA CCTGTGAGAG CCGCATCCAC ACCTCACTCA 
     1561 TTGGCTGCAT CAAAGCATTG ATGAACAACT CCCAGGGGCG GGCACATGTG CTGGCACAGC 
     1621 CTGAGGCCAT TAGTACCATA GCCCAGAGCC TACGCACAGA GAACAGCAAG ACCAAGGTGG 
     1681 CTGTGCTGGA GATCCTGGGT GCTGTGTGCC TCGTGCCTGG TGGCCACAAG AAGGTGCTGC 
     1741 AGGCCATGCT GCACTACCAG GTGTATGCAG CAGAGCGAAC CCGCTTCCAG ACCCTGCTGA 
     1801 ACGAGCTAGA CCGAAGTCTG GGCCGGTACC GGGATGAAGT GAATCTGAAA ACAGCCATCA 
     1861 TGTCCTTCAT CAATGCTGTC CTCAATGCTG GAGCTGGAGA GGATAATCTG GAGTTCCGCC 
     1921 TACATCTACG GTATGAATTC CTGATGCTGG GTATACAGCC TGTGATTGAC AAGCTCCGGC 
     1981 AACATGAAAA TGCCATCCTG GACAAACATT TAGACTTCTT CGAGATGGTG CGGAATGAGG 
     2041 ATGACCTGGA GCTAGCCAGG AGGTTTGACA TGGTCCACAT CGACACCAAG AGTGCTTCCC 
     2101 AGATGTTTGA GTTGATCCAC AAGAAGCTGA AGTACACGGA GGCCTACCCC TGCCTGCTCT 
     2161 CTGTGCTGCA CCACTGCCTG CAGATGCCCT ACAAACGGAA CGGTGGCTAC TTCCAGCAGT 
     2221 GGCAGCTCCT GGACCGCATC CTCCAGCAGA TTGTCCTCCA GGATGAGCGG GGTGTGGACC 
     2281 CTGACCTGGC TCCCTTGGAG AACTTCAATG TCAAGAACAT CGTCAACATG CTCATCAACG 
     2341 AGAATGAAGT GAAACAGTGG CGAGACCAGG CAGAGAAGTT CCGGAAAGAA CACATGGAGC 
     2401 TTGTGAGCCG TCTGGAGAGG AAGGAGCGGG AATGCGAGAC AAAGACATTG GAGAAGGAAG 
     2461 AGATGATGCG GACGCTGAAC AAAATGAAGG ACAAGCTGGC CCGGGAGTCC CAGGAGCTGC 
     2521 GCCAGGCTCG GGGACAAGTG GCAGAGCTGG TAGCCCAGCT CAGTGAACTC TCAACAGGCC 
     2581 CTGTATCTTC CCCACCACCC CCTGGGGGCC CACTCACCTT GTCTTCCTCA ATGACAACCA 
     2641 ATGACCTGCC TCCACCCCCT CCTCCTCTGC CCTTTGCCTG TTGTCCCCCT CCCCCACCAC 
     2701 CACCCCTTCC TCCCGGGGGA CCCCCGACTC CCCCAGGTGC CCCACCTTGC CTCGGCATGG 
     2761 GCCTGCCCCT CCCTCAGGAC CCCTACCCCA GCAGTGACGT CCCACTCAGG AAAAAGCGTG 
     2821 TCCCCCAGCC TTCTCACCCA CTGAAGTCCT TCAACTGGGT GAAGCTGAAT GAGGAGCGTG 
     2881 TCCCTGGCAC CGTATGGAAT GAGATTGATG ACATGCAGGT ATTTCGGATC CTGGACCTAG 
     2941 AGGATTTTGA AAAGATGTTT TCAGCCTACC AGAGGCACCA GAAAGAGCTG GGCTCCACTG 
     3001 AAGACATCTA CCTGGCTTCC CGCAAGGTCA AAGAGCTGTC GGTCATTGAT GGCCGGAGGG 
     3061 CCCAAAACTG CATCATCCTT CTTTCCAAGT TGAAGCTTTC TAACGAGGAG ATCCGGCAGG 
     3121 CCATCTTGAA GATGGATGAG CAGGAGGACC TTGCTAAGGA CATGCTGGAG CAGCTCCTCA 
     3181 AGTTCATCCC AGAGAAGAGT GACATTGACC TCCTGGAGGA GCACAAGCAT GAAATTGAGC 
     3241 GGATGGCCCG TGCTGACCGC TTCCTCTATG AAATGAGCAG GATTGACCAC TACCAGCAGC 
     3301 GACTGCAAGC CCTCTTCTTC AAGAAGAAAT TCCAGGAGCG GCTGGCTGAG GCAAAGCCCA 
     3361 AAGTGGAAGC CATCCTGTTG GCCTCCCGGG AGCTGGTCCG CAGCAAGCGT CTTAGACAGA 
     3421 TGCTAGAGGT CATCCTAGCC ATAGGCAACT TCATGAACAA AGGGCAGCGT GGGGGCGCCT 
     3481 ACGGGTTCCG GGTGGCCAGC CTCAACAAGA TCGCTGACAC CAAGTCCAGC ATCGACAGAA 
     3541 ACATCTCTCT GCTCCATTAC CTGATCATGA TCCTGGAGAA GCATTTTCCT GATATTCTAA 
     3601 ACATGCCTTC AGAGCTGCAA CATCTTCCAG AAGCTGCCAA AGTCAACCTA GCAGAACTGG 
     3661 AGAAGGAGGT GGGCAACCTC AGGAGGGGCC TGAGAGCGGT GGAGGTGCTG GAGTATCAGA 
     3721 GGCGCCAGGT ACGGGAGCCC AGTGACAAGT TTGTCCCTGT CATGAGCGAC TTCATCACGG 
     3781 TGTCCAGCTT CAGCTTCTCC GAGCTGGAGG ACCAGCTAAA TGAGGCCAGG GACAAGTTCG 
     3841 CCAAGGCCTT GATGCACTTC GGGGAGCATG ACAGCAAGAT GCAGCCAGAC GAATTCTTTG 
     3901 GCATCTTTGA TACCTTCTTG CAGGCCTTCT CAGAGGCCCG GCAGGATCTA GAGGCCATGA 
     3961 GGAGGAGGAA GGAGGAGGAG GAGCGGCGGG CGCGCATGGA AGCCATGCTG AAGGAGCAGA 
     4021 GGGAACGTGA GCGGTGGCAG CGGCAGCGGA AGGTCCTGGC TGCAGGCAGC TCGCTGGAGG 
     4081 AGGGAGGAGA GTTCGATGAC CTGGTGTCGG CCCTGCGCTC TGGGGAGGTC TTCGACAAGG 
     4141 ACTTATGCAA GCTCAAGCGC AGCCGCAAGC GATCAGGGAG CCAGGCCCTG GAAGTTACCC 
     4201 GGGAGCGGGC AATAAACCGG CTAAATTATA GCGGACCGAC GCGTACGCGG CCGCTCGAGC 
     4261 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4321 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4381 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4441 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4501 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4561 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4621 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4681 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4741 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4801 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4861 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4921 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4981 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5041 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5101 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5161 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5221 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5281 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5341 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5401 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5461 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5521 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5581 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5641 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5701 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5761 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5821 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5881 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5941 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6001 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6061 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6121 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6181 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6241 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6301 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6361 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6421 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6481 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6541 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6601 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6661 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6721 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6781 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6841 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6901 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6961 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7021 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7081 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7141 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7201 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7261 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7321 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7381 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7441 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7501 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7561 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7621 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7681 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7741 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7801 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7861 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7921 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7981 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8041 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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