LOCUS       RC220759                8154 bp    DNA     circular  SYN 
DEFINITION  SMG7 (Myc-DDK-tagged)-Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 2
ACCESSION   NM_201568
VERSION     NM_201568.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4311..4402 
                     /label="Myc-DDK"  
     misc_feature    4432..5017 
                     /label="PolyA signal"  
     misc_feature    4489..4508 
                     /label="Restriction Enzyme"  
     misc_feature    5293..5965 
                     /label="ColE1"  
     misc_feature    6395..7189 
                     /label="Kan/Neo"  
     misc_feature    7224..7526 
                     /label="SV40 ori"  
     misc_feature    7612..8089 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4293..4299 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4293 
                     /label="SMG7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCCTGCAG AGCGCGCAGT ACCTCCGGCA GGCAGAAGTC CTGAAGGCTG 
     1081 ACATGACAGA TTCTAAGCTG GGTCCAGCTG AAGTCTGGAC ATCCAGGCAG GCTCTGCAGG 
     1141 ACCTGTACCA GAAAATGCTA GTTACCGATT TGGAATACGC TTTAGACAAG AAAGTAGAAC 
     1201 AGGATCTCTG GAATCACGCC TTTAAGAATC AGATCACAAC ACTGCAAGGC CAGGCAAAGA 
     1261 ATCGAGCAAA TCCGAATCGG AGTGAAGTTC AGGCAAACCT TTCTCTGTTC CTAGAGGCAG 
     1321 CTAGTGGCTT CTATACTCAG TTATTACAAG AACTGTGTAC AGTATTTAAT GTAGATTTAC 
     1381 CATGCCGTGT GAAGTCTTCC CAATTGGGAA TTATCAGCAA TAAACAGACG CATACCAGCG 
     1441 CCATAGTGAA GCCACAGTCT AGCTCCTGTT CCTATATCTG CCAGCACTGC CTCGTCCACC 
     1501 TTGGAGACAT TGCTCGATAC AGAAACCAGA CCAGCCAGGC AGAGTCCTAC TATAGGCATG 
     1561 CAGCTCAGCT TGTCCCCTCC AATGGTCAGC CTTATAATCA GTTGGCTATC TTAGCTTCTT 
     1621 CCAAAGGAGA CCATCTGACC ACAATTTTCT ACTACTGCAG AAGCATTGCT GTGAAGTTCC 
     1681 CTTTCCCAGC TGCCTCCACT AATCTGCAAA AAGCACTTTC TAAAGCACTG GAAAGCCGAG 
     1741 ATGAGGTGAA AACCAAGTGG GGTGTTTCTG ACTTCATCAA GGCCTTTATT AAATTCCACG 
     1801 GTCATGTGTA CCTGAGTAAG AGCTTGGAAA AGTTGAGCCC TCTTCGAGAG AAATTGGAAG 
     1861 AACAGTTTAA GAGGCTGCTA TTCCAAAAAG CTTTCAACTC TCAGCAGTTA GTTCATGTCA 
     1921 CTGTCATTAA CCTGTTTCAA CTTCATCACC TTCGTGACTT TAGCAATGAA ACCGAGCAGC 
     1981 ACACTTATAG CCAAGATGAG CAGCTATGTT GGACACAGTT GCTGGCCCTC TTTATGTCTT 
     2041 TTCTTGGCAT CCTGTGCAAG TGTCCTCTAC AGAATGAGTC TCAGGAGGAG TCCTACAATG 
     2101 CCTATCCTCT TCCAGCAGTC AAGGTCTCCA TGGACTGGCT AAGACTCAGA CCCAGGGTCT 
     2161 TTCAGGAGGC AGTGGTGGAT GAAAGACAGT ACATTTGGCC CTGGTTGATT TCTCTTCTGA 
     2221 ATAGTTTCCA TCCCCATGAA GAGGACCTCT CAAGTATTAG TGCGACACCA CTTCCAGAGG 
     2281 AGTTTGAATT ACAAGGATTT TTGGCATTGA GACCTTCTTT CAGGAACTTG GATTTTTCCA 
     2341 AAGGTCACCA GGGTATTACA GGGGACAAAG AAGGCCAGCA ACGACGAATA CGACAGCAAC 
     2401 GCTTGATCTC TATAGGCAAA TGGATTGCTG ATAATCAGCC AAGGCTGATT CAGTGTGAAA 
     2461 ATGAGGTAGG GAAATTGTTG TTTATCACAG AAATCCCAGA ATTAATACTG GAAGACCCCA 
     2521 GTGAAGCCAA AGAGAACCTC ATTCTGCAAG AAACATCTGT GATAGAGTCG CTGGCTGCAG 
     2581 ATGGGAGCCC AGGGCTAAAA TCAGTGCTAT CTACAAGCCG AAATTTAAGC AACAACTGTG 
     2641 ACACAGGAGA GAAGCCAGTG GTTACCTTCA AAGAAAACAT TAAGACACGA GAAGTGAACA 
     2701 GAGACCAAGG AAGAAGTTTT CCTCCCAAAG AGGTAAAATC CCAGACAGAA CTAAGAAAGA 
     2761 CTCCAGTGTC TGAAGCCAGA AAAACACCTG TAACTCAAAC CCCAACTCAA GCAAGTAACT 
     2821 CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC AGGCCAGGGT 
     2881 TTCCGCCCCC AACATATGTT ATCCCCCCGC CTGTGGCATT TTCTATGGGC TCAGGTTACA 
     2941 CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT CACTCTCCAG 
     3001 CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG CAACGGCCCT 
     3061 CTGGACCAGG GCCAATGAAC CAGGGACCTC AACAATCACA GCCACCTTCC CAGCAACCCC 
     3121 TTACATCTTT ACCAGCTCAG CCAACAGCAC AGTCTACAAG CCAGCTGCAG GTTCAAGCTC 
     3181 TAACTCAGCA ACAACAATCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA AGCCCGCCTC 
     3241 ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG TGGAATCCCC 
     3301 CTCAGGTTCA AGGCCCATTA GGGAAAATTA TGCCTGTGAA ACAGCCCTAC TACCTTCAGA 
     3361 CCCAAGACCC CATAAAACTG TTTGAGCCGT CATTGCAACC TCCTGTAATG CAGCAGCAGC 
     3421 CTCTAGAAAA AAAAATGAAG CCTTTTCCCA TGGAGCCATA TAACCATAAT CCCTCAGAAG 
     3481 TCAAGGTCCC AGAATTCTAC TGGGATTCTT CCTACAGCAT GGCTGATAAC AGATCTGTAA 
     3541 TGGCACAGCA AGCAAACATA GACCGCAGGG GCAAACGGTC ACCAGGAGTC TTCCGTCCAG 
     3601 AGCAGGATCC TGTACCCAGA ATGCCGTTTG AGAAATCCTT ATTGGAGAAG CCCTCAGAGC 
     3661 TCATGTCACA TTCATCCTCT TTCCTGTCCC TCACCGGATT CTCTCTCAAT CAGGAAAGAT 
     3721 ACCCAAATAA TAGTATGTTC AATGAGGTAT ATGGGAAAAA CCTGACATCC AGCTCCAAAG 
     3781 CAGAACTCAG TCCCTCAATG GCCCCCCAGG AAACATCTCT GTATTCCCTT TTTGAAGGGA 
     3841 CTCCGTGGTC TCCATCACTT CCTGCCAGTT CAGATCATTC AACACCAGCC AGCCAGTCTC 
     3901 CTCATTCCTC TAACCCAAGC AGCCTACCCA GCTCTCCTCC AACACACAAC CATAATTCTG 
     3961 TTCCATTCTC CAATTTTGGA CCCATTGGGA CTCCAGATAA CAGGGATAGA AGGACTGCAG 
     4021 ATCGGTGGAA AACTGATAAG CCAGCCATGG GTGGGTTTGG CATTGATTAT CTCTCAGCAA 
     4081 CGTCATCCTC TGAGAGCAGT TGGCATCAGG CCAGCACTCC GAGTGGCACC TGGACAGGCC 
     4141 ATGGCCCTTC CATGGAGGAT TCCTCTGCTG TCCTCATGGA AAGCCTAAAG TCTATCTGGT 
     4201 CCAGTTCCAT GATGCATCCT GGACCTTCTG CTCTGGAGCA GCTGTTAATG CAGCAGAAGC 
     4261 AGAAACAGCA ACGGGGACAA GGCACCATGA ACCCTCCACA CACGCGTACG CGGCCGCTCG 
     4321 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4381 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4441 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4501 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4561 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4621 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4681 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4741 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4801 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4861 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4921 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4981 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5041 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5101 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5161 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5221 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5281 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5341 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5401 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5461 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5521 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5581 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5641 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5701 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5761 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5821 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5881 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5941 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6001 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6061 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6121 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6181 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6241 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6301 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6361 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6421 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6481 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6541 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6601 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6661 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6721 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6781 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6841 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6901 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6961 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7021 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7081 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7141 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7201 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7261 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7321 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7381 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7441 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7501 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7561 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7621 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7681 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7741 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7801 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7861 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7921 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7981 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8041 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8101 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//