LOCUS       RC220707                8574 bp    DNA     circular  SYN 
DEFINITION  CFH (Myc-DDK-tagged)-Human complement factor H (CFH), transcript variant 1
ACCESSION   NM_000186
VERSION     NM_000186.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4731..4822 
                     /label="Myc-DDK"  
     misc_feature    4852..5437 
                     /label="PolyA signal"  
     misc_feature    4909..4928 
                     /label="Restriction Enzyme"  
     misc_feature    5713..6385 
                     /label="ColE1"  
     misc_feature    6815..7609 
                     /label="Kan/Neo"  
     misc_feature    7644..7946 
                     /label="SV40 ori"  
     misc_feature    8032..8509 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4713..4719 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4713 
                     /label="CFH"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGACTTCTA GCAAAGATTA TTTGCCTTAT GTTATGGGCT ATTTGTGTAG 
     1081 CAGAAGATTG CAATGAACTT CCTCCAAGAA GAAATACAGA AATTCTGACA GGTTCCTGGT 
     1141 CTGACCAAAC ATATCCAGAA GGCACCCAGG CTATCTATAA ATGCCGCCCT GGATATAGAT 
     1201 CTCTTGGAAA TGTAATAATG GTATGCAGGA AGGGAGAATG GGTTGCTCTT AATCCATTAA 
     1261 GGAAATGTCA GAAAAGGCCC TGTGGACATC CTGGAGATAC TCCTTTTGGT ACTTTTACCC 
     1321 TTACAGGAGG AAATGTGTTT GAATATGGTG TAAAAGCTGT GTATACATGT AATGAGGGGT 
     1381 ATCAATTGCT AGGTGAGATT AATTACCGTG AATGTGACAC AGATGGATGG ACCAATGATA 
     1441 TTCCTATATG TGAAGTTGTG AAGTGTTTAC CAGTGACAGC ACCAGAGAAT GGAAAAATTG 
     1501 TCAGTAGTGC AATGGAACCA GATCGGGAAT ACCATTTTGG ACAAGCAGTA CGGTTTGTAT 
     1561 GTAACTCAGG CTACAAGATT GAAGGAGATG AAGAAATGCA TTGTTCAGAC GATGGTTTTT 
     1621 GGAGTAAAGA GAAACCAAAG TGTGTGGAAA TTTCATGCAA ATCCCCAGAT GTTATAAATG 
     1681 GATCTCCTAT ATCTCAGAAG ATTATTTATA AGGAGAATGA ACGATTTCAA TATAAATGTA 
     1741 ACATGGGTTA TGAATACAGT GAAAGAGGAG ATGCTGTATG CACTGAATCT GGATGGCGTC 
     1801 CGTTGCCTTC ATGTGAAGAA AAATCATGTG ATAATCCTTA TATTCCAAAT GGTGACTACT 
     1861 CACCTTTAAG GATTAAACAC AGAACTGGAG ATGAAATCAC GTACCAGTGT AGAAATGGTT 
     1921 TTTATCCTGC AACCCGGGGA AATACAGCAA AATGCACAAG TACTGGCTGG ATACCTGCTC 
     1981 CGAGATGTAC CTTGAAACCT TGTGATTATC CAGACATTAA ACATGGAGGT CTATATCATG 
     2041 AGAATATGCG TAGACCATAC TTTCCAGTAG CTGTAGGAAA ATATTACTCC TATTACTGTG 
     2101 ATGAACATTT TGAGACTCCG TCAGGAAGTT ACTGGGATCA CATTCATTGC ACACAAGATG 
     2161 GATGGTCGCC AGCAGTACCA TGCCTCAGAA AATGTTATTT TCCTTATTTG GAAAATGGAT 
     2221 ATAATCAAAA TCATGGAAGA AAGTTTGTAC AGGGTAAATC TATAGACGTT GCCTGCCATC 
     2281 CTGGCTACGC TCTTCCAAAA GCGCAGACCA CAGTTACATG TATGGAGAAT GGCTGGTCTC 
     2341 CTACTCCCAG ATGCATCCGT GTCAAAACAT GTTCCAAATC AAGTATAGAT ATTGAGAATG 
     2401 GGTTTATTTC TGAATCTCAG TATACATATG CCTTAAAAGA AAAAGCGAAA TATCAATGCA 
     2461 AACTAGGATA TGTAACAGCA GATGGTGAAA CATCAGGATC AATTACATGT GGGAAAGATG 
     2521 GATGGTCAGC TCAACCCACG TGCATTAAAT CTTGTGATAT CCCAGTATTT ATGAATGCCA 
     2581 GAACTAAAAA TGACTTCACA TGGTTTAAGC TGAATGACAC ATTGGACTAT GAATGCCATG 
     2641 ATGGTTATGA AAGCAATACT GGAAGCACCA CTGGTTCCAT AGTGTGTGGT TACAATGGTT 
     2701 GGTCTGATTT ACCCATATGT TATGAAAGAG AATGCGAACT TCCTAAAATA GATGTACACT 
     2761 TAGTTCCTGA TCGCAAGAAA GACCAGTATA AAGTTGGAGA GGTGTTGAAA TTCTCCTGCA 
     2821 AACCAGGATT TACAATAGTT GGACCTAATT CCGTTCAGTG CTACCACTTT GGATTGTCTC 
     2881 CTGACCTCCC AATATGTAAA GAGCAAGTAC AATCATGTGG TCCACCTCCT GAACTCCTCA 
     2941 ATGGGAATGT TAAGGAAAAA ACGAAAGAAG AATATGGACA CAGTGAAGTG GTGGAATATT 
     3001 ATTGCAATCC TAGATTTCTA ATGAAGGGAC CTAATAAAAT TCAATGTGTT GATGGAGAGT 
     3061 GGACAACTTT ACCAGTGTGT ATTGTGGAGG AGAGTACCTG TGGAGATATA CCTGAACTTG 
     3121 AACATGGCTG GGCCCAGCTT TCTTCCCCTC CTTATTACTA TGGAGATTCA GTGGAATTCA 
     3181 ATTGCTCAGA ATCATTTACA ATGATTGGAC ACAGATCAAT TACGTGTATT CATGGAGTAT 
     3241 GGACCCAACT TCCCCAGTGT GTGGCAATAG ATAAACTTAA GAAGTGCAAA TCATCAAATT 
     3301 TAATTATACT TGAGGAACAT TTAAAAAACA AGAAGGAATT CGATCATAAT TCTAACATAA 
     3361 GGTACAGATG TAGAGGAAAA GAAGGATGGA TACACACAGT CTGCATAAAT GGAAGATGGG 
     3421 ATCCAGAAGT GAACTGCTCA ATGGCACAAA TACAATTATG CCCACCTCCA CCTCAGATTC 
     3481 CCAATTCTCA CAATATGACA ACCACACTGA ATTATCGGGA TGGAGAAAAA GTATCTGTTC 
     3541 TTTGCCAAGA AAATTATCTA ATTCAGGAAG GAGAAGAAAT TACATGCAAA GATGGAAGAT 
     3601 GGCAGTCAAT ACCACTCTGT GTTGAAAAAA TTCCATGTTC ACAACCACCT CAGATAGAAC 
     3661 ACGGAACCAT TAATTCATCC AGGTCTTCAC AAGAAAGTTA TGCACATGGG ACTAAATTGA 
     3721 GTTATACTTG TGAGGGTGGT TTCAGGATAT CTGAAGAAAA TGAAACAACA TGCTACATGG 
     3781 GAAAATGGAG TTCTCCACCT CAGTGTGAAG GCCTTCCTTG TAAATCTCCA CCTGAGATTT 
     3841 CTCATGGTGT TGTAGCTCAC ATGTCAGACA GTTATCAGTA TGGAGAAGAA GTTACGTACA 
     3901 AATGTTTTGA AGGTTTTGGA ATTGATGGGC CTGCAATTGC AAAATGCTTA GGAGAAAAAT 
     3961 GGTCTCACCC TCCATCATGC ATAAAAACAG ATTGTCTCAG TTTACCTAGC TTTGAAAATG 
     4021 CCATACCCAT GGGAGAGAAG AAGGATGTGT ATAAGGCGGG TGAGCAAGTG ACTTACACTT 
     4081 GTGCAACATA TTACAAAATG GATGGAGCCA GTAATGTAAC ATGCATTAAT AGCAGATGGA 
     4141 CAGGAAGGCC AACATGCAGA GACACCTCCT GTGTGAATCC GCCCACAGTA CAAAATGCTT 
     4201 ATATAGTGTC GAGACAGATG AGTAAATATC CATCTGGTGA GAGAGTACGT TATCAATGTA 
     4261 GGAGCCCTTA TGAAATGTTT GGGGATGAAG AAGTGATGTG TTTAAATGGA AACTGGACGG 
     4321 AACCACCTCA ATGCAAAGAT TCTACAGGAA AATGTGGGCC CCCTCCACCT ATTGACAATG 
     4381 GGGACATTAC TTCATTCCCG TTGTCAGTAT ATGCTCCAGC TTCATCAGTT GAGTACCAAT 
     4441 GCCAGAACTT GTATCAACTT GAGGGTAACA AGCGAATAAC ATGTAGAAAT GGACAATGGT 
     4501 CAGAACCACC AAAATGCTTA CATCCGTGTG TAATATCCCG AGAAATTATG GAAAATTATA 
     4561 ACATAGCATT AAGGTGGACA GCCAAACAGA AGCTTTATTC GAGAACAGGT GAATCAGTTG 
     4621 AATTTGTGTG TAAACGGGGA TATCGTCTTT CATCACGTTC TCACACATTG CGAACAACAT 
     4681 GTTGGGATGG GAAACTGGAG TATCCAACTT GTGCAAAAAG AACGCGTACG CGGCCGCTCG 
     4741 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4801 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4861 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4921 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4981 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5041 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5101 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5161 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5221 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5281 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5341 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5401 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5461 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5521 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5581 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5641 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5701 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5761 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5821 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5881 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5941 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6001 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6061 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6121 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6181 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6241 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6301 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6361 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6421 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6481 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6541 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6601 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6661 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6721 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6781 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6841 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6901 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6961 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7021 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7081 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7141 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7201 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7261 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7321 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7381 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7441 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7501 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7561 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7621 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7681 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7741 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7801 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7861 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7921 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7981 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8041 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8101 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8161 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8221 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8281 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8341 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8401 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8461 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8521 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//