LOCUS       RC220705                7845 bp    DNA     circular  SYN 
DEFINITION  SCUBE1 (Myc-DDK-tagged)-Human signal peptide, CUB domain, EGF-like 1 (SCUBE1)
ACCESSION   NM_173050
VERSION     NM_173050.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4002..4093 
                     /label="Myc-DDK"  
     misc_feature    4123..4708 
                     /label="PolyA signal"  
     misc_feature    4180..4199 
                     /label="Restriction Enzyme"  
     misc_feature    4984..5656 
                     /label="ColE1"  
     misc_feature    6086..6880 
                     /label="Kan/Neo"  
     misc_feature    6915..7217 
                     /label="SV40 ori"  
     misc_feature    7303..7780 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3984..3990 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3984 
                     /label="SCUBE1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGCGGCG GCCGTGCGCT GGCACTTGTG CGTGCTGCTG GCCCTGGGCA 
     1081 CACGCGGGCG GCTGGCCGGG GGCAGCGGGC TCCCAGGGTC AGTCGACGTG GATGAGTGCT 
     1141 CAGAGGGCAC AGATGACTGC CACATCGATG CCATCTGTCA GAACACGCCC AAGTCCTACA 
     1201 AATGCCTCTG CAAGCCAGGC TACAAGGGGG AAGGCAAGCA GTGTGAAGAC ATTGACGAGT 
     1261 GTGAGAATGA CTACTACAAT GGGGGCTGTG TCCACGAGTG CATCAACATC CCGGGGAACT 
     1321 ACAGGTGTAC CTGCTTTGAT GGCTTCATGC TGGCACACGA TGGACACAAC TGCCTGGATG 
     1381 TGGACGAGTG TCAGGACAAT AATGGTGGCT GCCAGCAGAT CTGCGTCAAT GCCATGGGCA 
     1441 GCTACGAGTG TCAGTGCCAC AGTGGCTTCT TCCTTAGTGA CAACCAGCAT ACCTGCATCC 
     1501 ACCGCTCCAA TGAGGGTATG AACTGCATGA ACAAAGACCA TGGCTGTGCC CACATCTGCC 
     1561 GGGAGACGCC CAAAGGTGGG GTGGCCTGCG ACTGCAGGCC CGGCTTTGAC CTTGCCCAAA 
     1621 ACCAGAAGGA CTGCACACTA ACCTGTAATT ATGGAAACGG AGGCTGCCAG CACAGCTGTG 
     1681 AGGACACAGA CACAGGCCCC ACGTGTGGTT GCCACCAGAA GTACGCCCTC CACTCAGACG 
     1741 GTCGCACGTG CATCGAGACG TGCGCAGTCA ATAACGGAGG CTGCGACCGG ACATGCAAGG 
     1801 ACACAGCCAC TGGCGTGCGA TGCAGCTGCC CCGTTGGATT CACACTGCAG CCGGACGGGA 
     1861 AGACATGCAA AGACATCAAC GAGTGCCTGG TCAACAACGG AGGCTGCGAC CACTTCTGCC 
     1921 GCAACACCGT GGGCAGCTTC GAGTGCGGCT GCCGGAAGGG CTACAAGCTG CTCACCGACG 
     1981 AGCGCACCTG CCAGGACATC GACGAGTGCT CCTTCGAGCG GACCTGTGAC CACATCTGCA 
     2041 TCAACTCCCC GGGCAGCTTC CAGTGCCTGT GTCACCGCGG CTACATCCTC TACGGGACAA 
     2101 CCCACTGCGG AGATGTGGAC GAGTGCAGCA TGAGCAACGG GAGCTGTGAC CAGGGCTGCG 
     2161 TCAACACCAA GGGCAGCTAC GAGTGCGTCT GTCCCCCGGG GAGGCGGCTC CACTGGAACG 
     2221 GGAAGGATTG CGTGGAGACA GGCAAGTGTC TTTCTCGCGC CAAGACCTCC CCCCGGGCCC 
     2281 AGCTGTCCTG CAGCAAGGCA GGCGGTGTGG AGAGCTGCTT CCTTTCCTGC CCGGCTCACA 
     2341 CACTCTTCGT GCCAGACTCG GAAAATAGCT ACGTCCTGAG CTGCGGAGTT CCAGGGCCGC 
     2401 AGGGCAAGGC GCTGCAGAAA CGCAACGGCA CCAGCTCTGG CCTCGGGCCC AGCTGCTCAG 
     2461 ATGCCCCCAC CACCCCCATC AAACAGAAGG CCCGCTTCAA GATCCGAGAT GCCAAGTGCC 
     2521 ACCTCCGGCC CCACAGCCAG GCACGAGCAA AGGAGACCGC CAGGCAGCCG CTGCTGGACC 
     2581 ACTGCCATGT GACTTTCGTG ACCCTCAAGT GTGACTCCTC CAAGAAGAGG CGCCGTGGCC 
     2641 GCAAGTCCCC ATCCAAGGAG GTGTCCCACA TCACAGCAGA GTTTGAGATC GAGACAAAGA 
     2701 TGGAAGAGGC CTCAGACACA TGCGAAGCGG ACTGCTTGCG GAAGCGAGCA GAACAGAGCC 
     2761 TGCAGGCCGC CATCAAGACC CTGCGCAAGT CCATCGGCCG GCAGCAGTTC TATGTCCAGG 
     2821 TCTCAGGCAC TGAGTACGAG GTAGCCCAGA GGCCAGCCAA GGCGCTGGAG GGGCAGGGGG 
     2881 CATGTGGCGC AGGCCAGGTG CTACAGGACA GCAAATGCGT TGCCTGTGGG CCTGGCACCC 
     2941 ACTTCGGTGG TGAGCTCGGC CAGTGTGTGT CATGTATGCC AGGAACATAC CAGGACATGG 
     3001 AAGGCCAGCT CAGTTGCACA CCGTGCCCCA GCAGCGACGG GCTTGGTCTG CCTGGTGCCC 
     3061 GCAACGTGTC GGAATGTGGA GGCCAGTGTT CTCCAGGCTT CTTCTCGGCC GATGGCTTCA 
     3121 AGCCCTGCCA GGCCTGCCCC GTGGGCACGT ACCAGCCTGA GCCCGGGCGC ACCGGCTGCT 
     3181 TCCCCTGTGG AGGGGGTTTG CTCACCAAAC ACGAAGGCAC CACCTCCTTC CAGGACTGCG 
     3241 AGGCTAAAGT GCACTGCTCC CCCGGCCACC ACTACAACAC CACCACCCAC CGCTGCATCC 
     3301 GCTGCCCCGT CGGCACCTAC CAGCCCGAGT TTGGCCAGAA CCACTGCATC ACCTGTCCGG 
     3361 GCAACACCAG CACAGACTTC GATGGCTCCA CCAACGTCAC ACACTGCAAA AACCAGCACT 
     3421 GCGGCGGCGA GCTTGGTGAC TACACCGGCT ACATCGAGTC CCCCAACTAC CCTGGCGACT 
     3481 ACCCAGCCAA CGCTGAATGC GTCTGGCACA TCGCACCTCC CCCAAAGCGC AGGATCCTCA 
     3541 TCGTGGTCCC TGAGATCTTC CTGCCCATCG AGGATGAGTG CGGCGATGTT CTGGTCATGA 
     3601 GGAAGAGTGC CTCTCCCACG TCCATCACCA CCTATGAGAC CTGCCAGACC TACGAGAGGC 
     3661 CCATCGCCTT CACCTCCCGC TCCCGCAAGC TCTGGATCCA GTTCAAATCC AATGAAGGCA 
     3721 ACAGCGGCAA AGGCTTCCAA GTGCCCTATG TCACCTACGA TGAGGACTAC CAGCAACTCA 
     3781 TAGAGGACAT CGTGCGCGAT GGGCGCCTGT ACGCCTCGGA GAACCACCAG GAAATTTTGA 
     3841 AAGACAAGAA GCTGATCAAG GCCCTCTTCG ACGTGCTGGC GCATCCCCAG AACTACTTCA 
     3901 AGTACACAGC CCAGGAATCC AAGGAGATGT TCCCACGGTC CTTCATCAAA CTGCTGCGCT 
     3961 CCAAAGTGTC TCGGTTCCTG CGGCCCTACA AAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4021 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4081 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4141 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4201 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4261 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4321 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4381 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4441 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4501 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4561 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4621 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4681 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4741 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4801 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4861 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4921 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4981 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5041 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5101 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5161 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5221 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5281 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5341 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5401 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5461 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5521 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5581 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5641 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5701 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5761 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5821 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5881 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5941 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6001 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6061 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6121 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6181 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6241 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6301 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6361 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6421 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6481 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6541 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6601 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6661 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6721 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6781 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6841 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6901 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6961 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7021 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7081 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7141 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7201 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7261 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7321 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7381 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7441 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7501 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7561 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7621 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7681 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7741 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7801 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//