LOCUS       RC220700                7938 bp    DNA     circular  SYN 
DEFINITION  IDE (Myc-DDK-tagged)-Human insulin-degrading enzyme (IDE), transcript variant 1
ACCESSION   NM_004969
VERSION     NM_004969.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4095..4186 
                     /label="Myc-DDK"  
     misc_feature    4216..4801 
                     /label="PolyA signal"  
     misc_feature    4273..4292 
                     /label="Restriction Enzyme"  
     misc_feature    5077..5749 
                     /label="ColE1"  
     misc_feature    6179..6973 
                     /label="Kan/Neo"  
     misc_feature    7008..7310 
                     /label="SV40 ori"  
     misc_feature    7396..7873 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4077..4083 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4077 
                     /label="IDE"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGTACCGG CTAGCGTGGC TTCTGCACCC CGCACTGCCC AGCACCTTCC 
     1081 GCTCAGTCCT CGGCGCCCGC CTGCCGCCTC CGGAGCGCCT GTGTGGTTTC CAAAAAAAGA 
     1141 CTTACAGCAA AATGAATAAT CCAGCCATCA AGAGAATAGG AAATCACATT ACCAAGTCTC 
     1201 CTGAAGACAA GCGAGAATAT CGAGGGCTAG AGCTGGCCAA TGGTATCAAA GTACTTCTTA 
     1261 TGAGTGATCC CACCACGGAT AAGTCATCAG CAGCACTTGA TGTGCACATA GGTTCATTGT 
     1321 CGGATCCTCC AAATATTGCT GGCTTAAGTC ATTTTTGTGA ACATATGCTT TTTTTGGGAA 
     1381 CAAAGAAATA CCCTAAAGAA AATGAATACA GCCAGTTTCT CAGTGAGCAT GCAGGAAGTT 
     1441 CAAATGCCTT TACTAGTGGA GAGCATACCA ATTACTATTT TGATGTTTCT CATGAACACC 
     1501 TAGAAGGTGC CCTAGACAGG TTTGCACAGT TTTTTCTGTG CCCCTTGTTC GATGAAAGTT 
     1561 GCAAAGACAG AGAGGTGAAT GCAGTTGATT CAGAACATGA GAAGAATGTG ATGAATGATG 
     1621 CCTGGAGACT CTTTCAATTG GAAAAAGCTA CAGGGAATCC TAAACACCCC TTCAGTAAAT 
     1681 TTGGGACAGG TAACAAATAT ACTCTGGAGA CTAGACCAAA CCAAGAAGGC ATTGATGTAA 
     1741 GACAAGAGCT ACTGAAATTC CATTCTGCTT ACTATTCATC CAACTTAATG GCTGTTTGTG 
     1801 TTTTAGGTCG AGAATCTTTA GATGACTTGA CTAATCTGGT GGTAAAGTTA TTTTCTGAAG 
     1861 TAGAGAACAA AAATGTTCCA TTGCCAGAAT TTCCTGAACA CCCTTTCCAA GAAGAACATC 
     1921 TTAAACAACT TTACAAAATA GTACCCATTA AAGATATTAG GAATCTCTAT GTGACATTTC 
     1981 CCATACCTGA CCTTCAGAAA TACTACAAAT CAAATCCTGG TCATTATCTT GGTCATCTCA 
     2041 TTGGGCATGA AGGTCCTGGA AGTCTGTTAT CAGAACTTAA GTCAAAGGGC TGGGTTAATA 
     2101 CTCTTGTTGG TGGGCAGAAG GAAGGAGCCC GAGGTTTTAT GTTTTTTATC ATTAATGTGG 
     2161 ACTTGACCGA GGAAGGATTA TTACATGTTG AAGATATAAT TTTGCACATG TTTCAATACA 
     2221 TTCAGAAGTT ACGTGCAGAA GGACCTCAAG AATGGGTTTT CCAAGAGTGC AAGGACTTGA 
     2281 ATGCTGTTGC TTTTAGGTTT AAAGACAAAG AGAGGCCACG GGGCTATACA TCTAAGATTG 
     2341 CAGGAATATT GCATTATTAT CCCCTAGAAG AGGTGCTCAC AGCGGAATAT TTACTGGAAG 
     2401 AATTTAGACC TGACTTAATA GAGATGGTTC TCGATAAACT CAGACCAGAA AATGTCCGGG 
     2461 TTGCCATAGT TTCTAAATCT TTTGAAGGAA AAACTGATCG CACAGAAGAG TGGTATGGAA 
     2521 CCCAGTACAA ACAAGAAGCT ATACCGGATG AAGTCATCAA GAAATGGCAA AATGCTGACC 
     2581 TGAATGGGAA ATTTAAACTT CCTACAAAGA ATGAATTTAT TCCTACGAAT TTTGAGATTT 
     2641 TACCGTTAGA AAAAGAGGCG ACACCATACC CTGCTCTTAT TAAGGATACA GTCATGAGCA 
     2701 AACTTTGGTT CAAACAAGAT GATAAGAAAA AAAAGCCGAA GGCTTGTCTC AACTTTGAAT 
     2761 TTTTCAGCCC ATTTGCTTAT GTGGACCCCT TGCACTGTAA CATGGCCTAT TTGTACCTTG 
     2821 AGCTCCTCAA AGACTCACTC AACGAGTATG CATATGCAGC AGAGCTAGCA GGCTTGAGCT 
     2881 ATGATCTCCA AAATACCATC TATGGGATGT ATCTTTCAGT GAAAGGTTAC AATGACAAGC 
     2941 AGCCAATTTT ACTAAAGAAG ATTATTGAGA AAATGGCTAC CTTTGAGATT GATGAAAAAA 
     3001 GATTTGAAAT TATCAAAGAA GCATATATGC GATCTCTTAA CAATTTCCGG GCTGAACAGC 
     3061 CTCACCAGCA TGCCATGTAC TACCTCCGCT TGCTGATGAC TGAAGTGGCC TGGACTAAAG 
     3121 ATGAGTTAAA AGAAGCTCTG GATGATGTAA CCCTTCCTCG CCTTAAGGCC TTCATACCTC 
     3181 AGCTCCTGTC ACGGCTGCAC ATTGAAGCCC TTCTCCATGG AAACATAACA AAGCAGGCTG 
     3241 CATTAGGAAT TATGCAGATG GTTGAAGACA CCCTCATTGA ACATGCTCAT ACCAAACCTC 
     3301 TCCTTCCAAG TCAGCTGGTT CGGTATAGAG AAGTTCAGCT CCCTGACAGA GGATGGTTTG 
     3361 TTTATCAGCA GAGAAATGAA GTTCACAATA ACTGTGGCAT CGAGATATAC TACCAAACAG 
     3421 ACATGCAAAG CACCTCAGAG AATATGTTTC TGGAGCTCTT CTGTCAGATT ATCTCGGAAC 
     3481 CTTGCTTCAA CACCCTGCGC ACCAAGGAGC AGTTGGGCTA TATCGTCTTC AGCGGGCCAC 
     3541 GTCGAGCTAA TGGCATACAG AGCTTGAGAT TCATCATCCA GTCAGAAAAG CCACCTCACT 
     3601 ACCTAGAAAG CAGAGTGGAA GCTTTCTTAA TTACCATGGA AAAGTCCATA GAGGACATGA 
     3661 CAGAAGAGGC CTTCCAAAAA CACATTCAGG CATTAGCAAT TCGTCGACTA GACAAACCAA 
     3721 AGAAGCTATC TGCTGAGTGT GCTAAATACT GGGGAGAAAT CATCTCCCAG CAATATAATT 
     3781 TTGACAGAGA TAACACTGAG GTTGCATATT TAAAGACACT TACCAAGGAA GATATCATCA 
     3841 AATTCTACAA GGAAATGTTG GCAGTAGATG CTCCAAGGAG ACATAAGGTA TCCGTCCATG 
     3901 TTCTTGCCAG GGAAATGGAT TCTTGTCCTG TTGTTGGAGA GTTCCCATGT CAAAATGACA 
     3961 TAAATTTGTC ACAAGCACCA GCCTTGCCAC AACCTGAAGT GATTCAGAAC ATGACCGAAT 
     4021 TCAAGCGTGG TCTGCCACTG TTTCCCCTTG TGAAACCACA TATTAACTTC ATGGCTGCAA 
     4081 AACTCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4141 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4201 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4261 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4321 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4381 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4441 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4501 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4561 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4621 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4681 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4741 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4801 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4861 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4921 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4981 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5041 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5101 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5161 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5221 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5281 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5341 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5401 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5461 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5521 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5581 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5641 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5701 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5761 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5821 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5881 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5941 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6001 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6061 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6121 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6181 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6241 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6301 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6361 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6421 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6481 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6541 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6601 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6661 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6721 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6781 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6841 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6901 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6961 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7021 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7081 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7141 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7201 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7261 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7321 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7381 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7441 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7501 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7561 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7621 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7681 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7741 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7801 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7861 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7921 AAATTTAACG CGAATTTT
//