LOCUS       RC220699                8904 bp    DNA     circular  SYN 
DEFINITION  ACIN1 (Myc-DDK-tagged)-Human apoptotic chromatin condensation inducer 1 (ACIN1), transcript variant 1
ACCESSION   NM_014977
VERSION     NM_014977.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5061..5152 
                     /label="Myc-DDK"  
     misc_feature    5182..5767 
                     /label="PolyA signal"  
     misc_feature    5239..5258 
                     /label="Restriction Enzyme"  
     misc_feature    6043..6715 
                     /label="ColE1"  
     misc_feature    7145..7939 
                     /label="Kan/Neo"  
     misc_feature    7974..8276 
                     /label="SV40 ori"  
     misc_feature    8362..8839 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5043..5049 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5043 
                     /label="ACIN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGAGACGG AAACATCCGA GGACATCCGG AGGAACCCGG GGAGTTCTGA 
     1081 GTGGTAATCG AGGGGTAGAG TATGGCAGTG GGCGGGGTCA TCTCGGTACG TTCGAAGGGC 
     1141 GTTGGCGGAA ATTACCGAAG ATGCCCGAAG CCGTCGGGAC GGACCCGAGT ACCTCACGCA 
     1201 AGATGGCGGA GCTGGAGGAG GTGACTCTGG ACGGGAAGCC TCTTCAGGCG CTGCGGGTGA 
     1261 CCGACCTGAA GGCCGCACTG GAGCAGCGAG GCCTAGCCAA GAGCGGGCAG AAGAGTGCCC 
     1321 TGGTCAAGCG GCTCAAAGGG GCTCTAATGC TAGAAAATTT ACAGAAACAC TCAACACCCC 
     1381 ATGCTGCATT CCAGCCAAAT TCCCAGATTG GTGAGGAAAT GAGCCAGAAC AGTTTCATAA 
     1441 AACAGTATCT GGAAAAGCAG CAGGAGCTAC TTAGGCAGCG TCTGGAACGT GAAGCTCGAG 
     1501 AAGCTGCAGA ACTTGAAGAA GCTTCAGCTG AGTCGGAGGA CGAGATGATC CATCCTGAGG 
     1561 GAGTGGCTTC CCTGCTGCCT CCTGACTTTC AGAGCAGCCT GGAGAGACCA GAGCTGGAGC 
     1621 TCAGCAGACA TTCGCCCAGA AAAAGCTCCT CAATTTCTGA AGAGAAAGGT GACTCTGATG 
     1681 ATGAGAAACC AAGGAAAGGA GAAAGACGAT CATCTAGGGT CAGACAGGCA AGAGCAGCTA 
     1741 AACTGTCTGA GGGCAGCCAA CCTGCTGAGG AGGAAGAGGA TCAAGAAACA CCTTCCAGAA 
     1801 ACCTAAGGGT CAGAGCAGAT CGAAATTTGA AAACAGAGGA GGAAGAAGAG GAGGAGGAGG 
     1861 AGGAGGAAGA AGATGATGAA GAAGAGGAAG GTGATGATGA GGGACAAAAA TCTAGGGAGG 
     1921 CACCAATCCT GAAAGAGTTT AAGGAAGAAG GGGAAGAGAT ACCTAGAGTA AAACCAGAGG 
     1981 AGATGATGGA TGAGAGACCC AAAACAAGAT CCCAGGAACA GGAGGTGTTA GAGAGAGGAG 
     2041 GGAGATTTAC AAGATCCCAG GAAGAGGCTA GAAAAAGTCA TCTGGCCAGA CAGCAGCAGG 
     2101 AGAAGGAAAT GAAAACAACA TCTCCCCTTG AGGAGGAAGA AAGAGAAATA AAATCTTCAC 
     2161 AAGGCTTAAA GGAAAAATCG AAGTCTCCTT CCCCTCCTCG ACTGACTGAA GATCGAAAGA 
     2221 AGGCCTCACT TGTAGCGCTG CCAGAGCAAA CTGCCAGCGA GGAGGAGACT CCTCCACCTT 
     2281 TACTAACAAA GGAAGCATCT TCTCCACCAC CTCATCCACA GCTCCATAGC GAAGAAGAAA 
     2341 TAGAGCCCAT GGAAGGCCCA GCCCCCCCTG TCCTCATTCA GTTATCTCCT CCTAATACAG 
     2401 ATGCTGACAC CAGGGAGCTA TTAGTATCTC AGCATACTGT CCAGTTGGTA GGAGGCCTGT 
     2461 CTCCTTTGTC AAGTCCTTCA GACACCAAAG CAGAATCTCC AGCAGAGAAA GTGCCAGAGG 
     2521 AGAGTGTCCT GCCTCTGGTT CAGAAAAGCA CACTGGCTGA CTACTCAGCC CAGAAGGATC 
     2581 TTGAACCTGA GTCAGACAGA TCTGCTCAGC CCCTCCCTCT AAAAATTGAG GAATTAGCAC 
     2641 TGGCCAAAGG AATCACTGAA GAATGTCTGA AACAGCCATC TTTGGAACAG AAGGAAGGCA 
     2701 GAAGAGCTTC TCATACCCTT CTCCCAAGCC ACAGATTGAA ACAGTCAGCT GATTCATCCT 
     2761 CTAGCCGGTC CTCCTCATCT TCCTCCTCCA GTTCTAGATC AAGATCTCGC TCTCCTGACA 
     2821 GTTCAGGTTC TCGGTCTCAT TCACCGCTCA GATCCAAGCA GAGAGATGTA GCCCAGGCAC 
     2881 GTACTCATGC CAACCCTCGT GGTAGACCCA AGATGGGCTC CAGATCAACA TCAGAGTCCA 
     2941 GATCAAGGTC ACGTTCACGT TCTCGTTCAG CATCAAGCAA CAGCAGAAAA TCTCTGAGCC 
     3001 CTGGAGTCTC CAGGGACAGC AGCACCAGCT ATACTGAAAC CAAAGATCCC TCTTCTGGTC 
     3061 AGGAGGTTGC AACTCCACCA GTGCCACAAC TGCAGGTCTG TGAGCCAAAG GAGAGGACTT 
     3121 CCACCTCCTC ATCCTCTGTC CAAGCAAGGC GTCTGAGTCA GCCTGAATCA GCTGAAAAGC 
     3181 ATGTGACCCA GAGGTTACAG CCTGAGCGGG GGAGCCCAAA GAAGTGTGAA GCTGAAGAGG 
     3241 CAGAGCCACC AGCTGCCACA CAGCCCCAAA CCTCAGAGAC TCAGACCTCT CATCTGCCAG 
     3301 AATCAGAAAG AATTCATCAC ACTGTTGAGG AGAAGGAGGA AGTGACCATG GACACAAGTG 
     3361 AAAACAGACC TGAAAATGAT GTTCCAGAAC CTCCCATGCC TATTGCAGAC CAAGTCAGCA 
     3421 ATGATGACCG CCCGGAGGGC AGTGTTGAAG ATGAGGAGAA GAAAGAGAGC TCGCTGCCCA 
     3481 AATCATTCAA GAGGAAGATC TCCGTTGTCT CAGCTACCAA GGGGGTGCCA GCTGGAAACA 
     3541 GTGACACAGA GGGGGGCCAG CCTGGTCGGA AACGACGCTG GGGAGCCAGC ACAGCCACCA 
     3601 CACAGAAGAA ACCTTCCATC AGTATCACCA CTGAATCACT AAAGAGCCTC ATCCCCGACA 
     3661 TCAAACCCCT GGCGGGGCAG GAGGCTGTTG TGGATCTTCA TGCTGATGAC TCTCGCATCT 
     3721 CTGAGGATGA GACAGAGCGT AATGGCGATG ATGGGACCCA TGACAAGGGG CTGAAAATAT 
     3781 GCCGGACAGT CACTCAGGTA GTACCTGCAG AGGGCCAGGA GAATGGGCAG AGGGAAGAAG 
     3841 AGGAAGAAGA GAAGGAACCT GAAGCAGAAC CTCCTGTACC TCCCCAGGTG TCAGTAGAGG 
     3901 TGGCCTTGCC CCCACCTGCA GAGCATGAAG TAAAGAAAGT GACTTTAGGA GATACCTTAA 
     3961 CTCGACGTTC CATTAGCCAG CAGAAGTCCG GAGTTTCCAT TACCATTGAT GACCCAGTCC 
     4021 GAACTGCCCA GGTGCCCTCC CCACCCCGGG GCAAGATTAG CAACATTGTC CATATCTCCA 
     4081 ATTTGGTCCG TCCTTTCACT TTAGGCCAGC TAAAGGAGTT GTTGGGGCGC ACAGGAACCT 
     4141 TGGTGGAAGA GGCCTTCTGG ATTGACAAGA TCAAATCTCA TTGCTTTGTA ACGTACTCAA 
     4201 CAGTAGAGGA AGCTGTTGCC ACCCGCACAG CTCTGCACGG GGTCAAATGG CCCCAGTCCA 
     4261 ATCCCAAATT CCTTTGTGCT GACTATGCCG AGCAAGATGA GCTGGATTAT CACCGAGGCC 
     4321 TCTTGGTGGA CCGTCCCTCT GAAACTAAGA CAGAGGAGCA GGGAATACCA CGGCCCCTGC 
     4381 ACCCCCCACC CCCACCCCCG GTCCAGCCAC CACAGCACCC CCGGGCAGAG CAGCGGGAGC 
     4441 AGGAACGGGC AGTGCGGGAA CAGTGGGCAG AACGGGAACG GGAAATGGAG CGGCGGGAGC 
     4501 GGACTCGATC AGAGCGTGAA TGGGATCGGG ACAAAGTTCG AGAAGGGCCC CGTTCCCGAT 
     4561 CAAGGTCCCG TGACCGCCGC CGCAAGGAAC GTGCGAAGTC TAAAGAAAAG AAGAGTGAGA 
     4621 AGAAAGAGAA AGCCCAGGAG GAACCACCTG CCAAGCTGCT GGATGACCTT TTCCGAAAGA 
     4681 CCAAGGCAGC TCCCTGCATC TATTGGCTCC CACTGACTGA CAGCCAGATC GTTCAGAAAG 
     4741 AGGCAGAGCG GGCCGAACGG GCCAAGGAGC GGGAGAAGCG GCGAAAGGAG CAAGAAGAAG 
     4801 AAGAGCAAAA GGAGCGGGAG AAGGAAGCCG AGCGGGAACG GAACCGACAG CTGGAGCGAG 
     4861 AGAAACGTCG GGAGCACAGT CGGGAGAGGG ACAGGGAGAG AGAGAGAGAA AGGGAGCGGG 
     4921 ACAGGGGGGA CCGAGATCGG GATAGGGAAA GGGACCGAGA ACGAGGCAGG GAAAGGGATC 
     4981 GCAGGGACAC CAAGCGCCAC AGCAGAAGCC GGAGTCGGAG CACACCTGTG CGGGACCGGG 
     5041 GTGGGCGCCG CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5101 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5161 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5221 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5281 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5341 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5401 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5461 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5521 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5581 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5641 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5701 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5761 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5821 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5881 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5941 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6001 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6061 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6121 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6181 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6241 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6301 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6361 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6421 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6481 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6541 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6601 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6661 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6721 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6781 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6841 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6901 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6961 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7021 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7081 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7141 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7201 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7261 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7321 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7381 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7441 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7501 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7561 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7621 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7681 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7741 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7801 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7861 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7921 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7981 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8041 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8101 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8161 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8221 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8281 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8341 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8401 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8461 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8521 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8581 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8641 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8701 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8761 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8821 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8881 TAACAAAAAT TTAACGCGAA TTTT
//