LOCUS       RC220658                7020 bp    DNA     circular  SYN 
DEFINITION  SP3 (Myc-DDK-tagged)-Human Sp3 transcription factor (SP3), transcript variant 2
ACCESSION   NM_001017371
VERSION     NM_001017371.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3177..3268 
                     /label="Myc-DDK"  
     misc_feature    3298..3883 
                     /label="PolyA signal"  
     misc_feature    3355..3374 
                     /label="Restriction Enzyme"  
     misc_feature    4159..4831 
                     /label="ColE1"  
     misc_feature    5261..6055 
                     /label="Kan/Neo"  
     misc_feature    6090..6392 
                     /label="SV40 ori"  
     misc_feature    6478..6955 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3159..3165 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3159 
                     /label="SP3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCCGCCA TCGCCGGGCG ACGACGAGGA GGAGGCGGCC GCCGCAGCCG 
     1081 GGGCCCCCGC CGCCGCCGGA GCGACAGGTG ATTTGGCTTC TGCACAGTTA GGAGGAGCAC 
     1141 CAAACCGATG GGAGGTTTTG TCAGCCACAC CTACAACTAT AAAAGATGAA GCTGGTAATC 
     1201 TAGTCCAGAT TCCAAGTGCT GCTACTTCAA GTGGGCAGTA TGTTCTTCCC CTTCAGAATT 
     1261 TGCAGAATCA ACAAATATTT TCCGTTGCAC CAGGATCAGA TTCATCAAAT GGTGCAGTGT 
     1321 CCAGTGTTCA ATATCAAGTG ATACCACAGA TCCAGTCAGC AGATGGTCAG CAGGTTCAAA 
     1381 TTGGTTTCAC AGGCTCTTCA GATAATGGGG GTATAAATCA AGAAAGCAGT CAAATTCAGA 
     1441 TCATTCCTGG CTCTAATCAA ACCTTACTTG CCTCTGGAAC ACCTTCTGCT AACATCCAGA 
     1501 ATCTCATACC ACAGACTGGT CAAGTCCAGG TTCAGGGAGT TGCAATTGGT GGTTCATCTT 
     1561 TTCCTGGTCA AACCCAAGTA GTTGCTAATG TGCCTCTTGG TCTGCCAGGA AATATTACGT 
     1621 TTGTACCAAT CAATAGTGTC GATCTAGATT CTTTGGGACT CTCGGGCAGT TCTCAGACAA 
     1681 TGACTGCAGG CATTAATGCC GACGGACATT TGATAAACAC AGGACAAGCT ATGGATAGTT 
     1741 CAGACAATTC AGAAAGGACT GGTGAGCGGG TTTCTCCTGA TATTAATGAA ACTAATACTG 
     1801 ATACAGATTT ATTTGTGCCA ACATCCTCTT CATCACAGTT GCCTGTTACG ATAGATAGTA 
     1861 CAGGTATATT ACAACAAAAC ACAAATAGCT TGACTACATC TAGTGGGCAG GTTCATTCTT 
     1921 CAGATCTTCA GGGAAATTAT ATCCAGTCGC CTGTTTCTGA AGAGACACAG GCACAGAATA 
     1981 TTCAGGTTTC TACAGCACAG CCTGTTGTAC AGCATCTACA ACTTCAAGAG TCTCAGCAGC 
     2041 CAACCAGTCA AGCCCAAATT GTGCAAGGTA TTACACCACA GACAATCCAT GGTGTGCAAG 
     2101 CCAGTGGTCA AAATATATCA CAACAGGCTT TGCAAAATCT TCAGTTGCAG CTGAATCCTG 
     2161 GAACCTTTTT AATTCAGGCA CAGACAGTGA CCCCTTCTGG ACAGGTAACT TGGCAAACGT 
     2221 TTCAAGTACA AGGGGTCCAG AACTTGCAGA ATTTGCAAAT ACAGAATACT GCTGCCCAAC 
     2281 AAATAACTTT GACGCCTGTT CAAACCCTCA CACTTGGTCA AGTTGCGGCA GGTGGAGCCT 
     2341 TCACTTCAAC TCCAGTTAGT CTAAGCACTG GTCAGTTGCC AAATCTACAA ACAGTTACAG 
     2401 TGAACTCTAT AGATTCTGCT GGTATACAGC TACATCCAGG AGAGAATGCT GACAGTCCTG 
     2461 CAGATATTAG GATCAAGGAA GAAGAACCTG ATCCTGAAGA GTGGCAGCTC AGTGGTGATT 
     2521 CTACCTTGAA TACCAATGAC CTAACACACT TAAGAGTACA GGTGGTAGAT GAAGAAGGGG 
     2581 ACCAACAACA TCAAGAAGGA AAAAGACTTC GGAGGGTAGC TTGCACCTGT CCCAACTGTA 
     2641 AAGAAGGTGG TGGAAGAGGT ACCAATCTTG GGAAAAAGAA GCAACACATT TGTCATATAC 
     2701 CAGGATGTGG TAAAGTCTAT GGGAAGACCT CACATCTGAG AGCTCATCTG CGTTGGCATT 
     2761 CTGGAGAACG CCCTTTTGTT TGTAACTGGA TGTACTGTGG TAAAAGATTT ACTCGAAGTG 
     2821 ATGAATTACA GAGGCACAGA AGAACACATA CAGGTGAGAA GAAATTTGTT TGTCCAGAAT 
     2881 GTTCAAAACG CTTTATGAGA AGTGACCACC TTGCCAAACA TATTAAAACA CACCAGAATA 
     2941 AAAAAGGTAT TCACTCTAGC AGTACAGTGC TGGCATCTGT GGAAGCTGCG CGAGATGATA 
     3001 CTTTGATTAC TGCAGGAGGA ACAACGCTTA TCCTTGCAAA TATTCAACAA GGTTCTGTTT 
     3061 CAGGGATAGG AACTGTTAAT ACTTCCGCCA CCAGCAATCA AGATATCCTT ACCAACACTG 
     3121 AAATACCTTT ACAGCTTGTC ACAGTTTCTG GAAATGAGAC AATGGAGACG CGTACGCGGC 
     3181 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3241 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3301 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3361 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3421 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     3481 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     3541 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     3601 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     3661 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     3721 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     3781 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     3841 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     3901 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     3961 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4021 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4081 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4141 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4201 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4261 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4321 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4381 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     4441 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     4501 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     4561 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     4621 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     4681 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     4741 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     4801 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     4861 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     4921 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     4981 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5041 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5101 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5161 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5221 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5281 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5341 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     5401 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     5461 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     5521 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     5581 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     5641 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     5701 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     5761 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     5821 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     5881 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     5941 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6001 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6061 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6121 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6181 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6241 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6301 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6361 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     6421 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     6481 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     6541 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     6601 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     6661 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     6721 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     6781 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     6841 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     6901 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     6961 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//