LOCUS       RC220643                8877 bp    DNA     circular  SYN 
DEFINITION  NPC1L1 (Myc-DDK-tagged)-Human NPC1 (Niemann-Pick disease, type C1, gene)-like 1 (NPC1L1), transcript variant 2
ACCESSION   NM_001101648
VERSION     NM_001101648.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5034..5125 
                     /label="Myc-DDK"  
     misc_feature    5155..5740 
                     /label="PolyA signal"  
     misc_feature    5212..5231 
                     /label="Restriction Enzyme"  
     misc_feature    6016..6688 
                     /label="ColE1"  
     misc_feature    7118..7912 
                     /label="Kan/Neo"  
     misc_feature    7947..8249 
                     /label="SV40 ori"  
     misc_feature    8335..8812 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5016..5022 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5016 
                     /label="NPC1L1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGGCC GGCCTGAGGG GCTGGCTGCT GTGGGCCCTG CTCCTGCGCT 
     1081 TGGCCCAGAG TGAGCCTTAC ACAACCATCC ACCAGCCTGG CTACTGCGCC TTCTATGACG 
     1141 AATGTGGGAA GAACCCAGAG CTGTCTGGAA GCCTCATGAC ACTCTCCAAC GTGTCCTGCC 
     1201 TGTCCAACAC GCCGGCCCGC AAGATCACAG GTGATCACCT GATCCTATTA CAGAAGATCT 
     1261 GCCCCCGCCT CTACACCGGC CCCAACACCC AAGCCTGCTG CTCCGCCAAG CAGCTGGTAT 
     1321 CACTGGAAGC GAGTCTGTCG ATCACCAAGG CCCTCCTCAC CCGCTGCCCA GCCTGCTCTG 
     1381 ACAATTTTGT GAACCTGCAC TGCCACAACA CGTGCAGCCC CAATCAGAGC CTCTTCATCA 
     1441 ATGTGACCCG CGTGGCCCAG CTAGGGGCTG GACAACTCCC AGCTGTGGTG GCCTATGAGG 
     1501 CCTTCTACCA GCATAGCTTT GCCGAGCAGA GCTATGACTC CTGCAGCCGT GTGCGCGTCC 
     1561 CTGCAGCTGC CACGCTGGCT GTGGGCACCA TGTGTGGCGT GTATGGCTCT GCCCTTTGCA 
     1621 ATGCCCAGCG CTGGCTCAAC TTCCAGGGAG ACACAGGCAA TGGTCTGGCC CCACTGGACA 
     1681 TCACCTTCCA CCTCTTGGAG CCTGGCCAGG CCGTGGGGAG TGGGATTCAG CCTCTGAATG 
     1741 AGGGGGTTGC ACGTTGCAAT GAGTCCCAAG GTGACGACGT GGCGACCTGC TCCTGCCAAG 
     1801 ACTGTGCTGC ATCCTGTCCT GCCATAGCCC GCCCCCAGGC CCTCGACTCC ACCTTCTACC 
     1861 TGGGCCAGAT GCCGGGCAGT CTGGTCCTCA TCATCATCCT CTGCTCTGTC TTCGCTGTGG 
     1921 TCACCATCCT GCTTGTGGGA TTCCGTGTGG CCCCCGCCAG GGACAAAAGC AAGATGGTGG 
     1981 ACCCCAAGAA GGGCACCAGC CTCTCTGACA AGCTCAGCTT CTCCACCCAC ACCCTCCTTG 
     2041 GCCAGTTCTT CCAGGGCTGG GGCACGTGGG TGGCTTCGTG GCCTCTGACC ATCTTGGTGC 
     2101 TATCTGTCAT CCCGGTGGTG GCCTTGGCAG CGGGCCTGGT CTTTACAGAA CTCACTACGG 
     2161 ACCCCGTGGA GCTGTGGTCG GCCCCCAACA GCCAAGCCCG GAGTGAGAAA GCTTTCCATG 
     2221 ACCAGCATTT CGGCCCCTTC TTCCGAACCA ACCAGGTGAT CCTGACGGCT CCTAACCGGT 
     2281 CCAGCTACAG GTATGACTCT CTGCTGCTGG GGCCCAAGAA CTTCAGCGGA ATCCTGGACC 
     2341 TGGACTTGCT GCTGGAGCTG CTAGAGCTGC AGGAGAGGCT GCGGCACCTC CAGGTATGGT 
     2401 CGCCCGAAGC ACAGCGCAAC ATCTCCCTGC AGGACATCTG CTACGCCCCC CTCAATCCGG 
     2461 ACAATACCAG TCTCTACGAC TGCTGCATCA ACAGCCTCCT GCAGTATTTC CAGAACAACC 
     2521 GCACGCTCCT GCTGCTCACA GCCAACCAGA CACTGATGGG GCAGACCTCC CAAGTCGACT 
     2581 GGAAGGACCA TTTTCTGTAC TGTGCCAATG CCCCGCTCAC CTTCAAGGAT GGCACAGCCC 
     2641 TGGCCCTGAG CTGCATGGCT GACTACGGGG CCCCTGTCTT CCCCTTCCTT GCCATTGGGG 
     2701 GGTACAAAGG AAAGGACTAT TCTGAGGCAG AGGCCCTGAT CATGACGTTC TCCCTCAACA 
     2761 ATTACCCTGC CGGGGACCCC CGTCTGGCCC AGGCCAAGCT GTGGGAGGAG GCCTTCTTAG 
     2821 AGGAAATGCG AGCCTTCCAG CGTCGGATGG CTGGCATGTT CCAGGTCACG TTCATGGCTG 
     2881 AGCGCTCTCT GGAAGACGAG ATCAATCGCA CCACAGCTGA AGACCTGCCC ATCTTTGCCA 
     2941 CCAGCTACAT TGTCATATTC CTGTACATCT CTCTGGCCCT GGGCAGCTAT TCCAGCTGGA 
     3001 GCCGAGTGAT GGTGGACTCC AAGGCCACGC TGGGCCTCGG CGGGGTGGCC GTGGTCCTGG 
     3061 GAGCAGTCAT GGCTGCCATG GGCTTCTTCT CCTACTTGGG TATCCGCTCC TCCCTGGTCA 
     3121 TCCTGCAAGT GGTTCCTTTC CTGGTGCTGT CCGTGGGGGC TGATAACATC TTCATCTTTG 
     3181 TTCTCGAGTA CCAGAGGCTG CCCCGGAGGC CTGGGGAGCC ACGAGAGGTC CACATTGGGC 
     3241 GAGCCCTAGG CAGGGTGGCT CCCAGCATGC TGTTGTGCAG CCTCTCTGAG GCCATCTGCT 
     3301 TCTTCCTAGG GGCCCTGACC CCCATGCCAG CTGTGCGGAC CTTTGCCCTG ACCTCTGGCC 
     3361 TTGCAGTGAT CCTTGACTTC CTCCTGCAGA TGTCAGCCTT TGTGGCCCTG CTCTCCCTGG 
     3421 ACAGCAAGAG GCAGGAGGCC TCCCGGTTGG ACGTCTGCTG CTGTGTCAAG CCCCAGGAGC 
     3481 TGCCCCCGCC TGGCCAGGGA GAGGGGCTCC TGCTTGGCTT CTTCCAAAAG GCTTATGCCC 
     3541 CCTTCCTGCT GCACTGGATC ACTCGAGGTG TTGTGCTGCT GCTGTTTCTC GCCCTGTTCG 
     3601 GAGTGAGCCT CTACTCCATG TGCCACATCA GCGTGGGACT GGACCAGGAG CTGGCCCTGC 
     3661 CCAAGGACTC GTACCTGCTT GACTATTTCC TCTTTCTGAA CCGCTACTTC GAGGTGGGGG 
     3721 CCCCGGTGTA CTTTGTTACC ACCTTGGGCT ACAACTTCTC CAGCGAGGCT GGGATGAATG 
     3781 CCATCTGCTC CAGTGCAGGC TGCAACAACT TCTCCTTCAC CCAGAAGATC CAGTATGCCA 
     3841 CAGAGTTCCC TGAGCAGTCT TACCTGGCCA TCCCTGCCTC CTCCTGGGTG GATGACTTCA 
     3901 TTGACTGGCT GACCCCGTCC TCCTGCTGCC GCCTTTATAT ATCTGGCCCC AATAAGGACA 
     3961 AGTTCTGCCC CTCGACCGTC AACTCTCTGA ACTGCCTAAA GAACTGCATG AGCATCACGA 
     4021 TGGGCTCTGT GAGGCCCTCG GTGGAGCAGT TCCATAAGTA TCTTCCCTGG TTCCTGAACG 
     4081 ACCGGCCCAA CATCAAATGT CCCAAAGGCG GCCTGGCAGC ATACAGCACC TCTGTGAACT 
     4141 TGACTTCAGA TGGCCAGGTT TTAGCCTCCA GGTTCATGGC CTATCACAAG CCCCTGAAAA 
     4201 ACTCACAGGA TTACACAGAA GCTCTGCGGG CAGCTCGAGA GCTGGCAGCC AACATCACTG 
     4261 CTGACCTGCG GAAAGTGCCT GGAACAGACC CGGCTTTTGA GGTCTTCCCC TACACGATCA 
     4321 CCAATGTGTT TTATGAGCAG TACCTGACCA TCCTCCCTGA GGGGCTCTTC ATGCTCAGCC 
     4381 TCTGCCTTGT GCCCACCTTC GCTGTCTCCT GCCTCCTGCT GGGCCTGGAC CTGCGCTCCG 
     4441 GCCTCCTCAA CCTGCTCTCC ATTGTCATGA TCCTCGTGGA CACTGTCGGC TTCATGGCCC 
     4501 TGTGGGGCAT CAGTTACAAT GCTGTGTCCC TCATCAACCT GGTCTCGGCG GTGGGCATGT 
     4561 CTGTGGAGTT TGTGTCCCAC ATTACCCGCT CCTTTGCCAT CAGCACCAAG CCCACCTGGC 
     4621 TGGAGAGGGC CAAAGAGGCC ACCATCTCTA TGGGAAGTGC GGTGTTTGCA GGTGTGGCCA 
     4681 TGACCAACCT GCCTGGCATC CTTGTCCTGG GCCTCGCCAA GGCCCAGCTC ATTCAGATCT 
     4741 TCTTCTTCCG CCTCAACCTC CTGATCACTC TGCTGGGCCT GCTGCATGGC TTGGTCTTCC 
     4801 TGCCCGTCAT CCTCAGCTAT GTGGGGCCTG ACGTCAACCC GGCTCTGGCA CTGGAGCAGA 
     4861 AGCGGGCTGA GGAGGCGGTG GCAGCAGTCA TGGTGGCCTC TTGCCCAAAT CACCCCTCCC 
     4921 GAGTCTCCAC AGCTGACAAC ATCTATGTCA ACCACAGCTT TGAAGGTTCT ATCAAAGGTG 
     4981 CTGGTGCCAT CAGCAACTTC TTGCCCAACA ATGGGCGGCA GTTCACGCGT ACGCGGCCGC 
     5041 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5101 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5161 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5221 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5281 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5341 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5401 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5461 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5521 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5581 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5641 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5701 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5761 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5821 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5881 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5941 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6001 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6061 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6121 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6181 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6241 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6301 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6361 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6421 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6481 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6541 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6601 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6661 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6721 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6781 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6841 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6901 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6961 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7021 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7081 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7141 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7201 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7261 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7321 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7381 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7441 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7501 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7561 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7621 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7681 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7741 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7801 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7861 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7921 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7981 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8041 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8101 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8161 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8221 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8281 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8341 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8401 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8461 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8521 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8581 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8641 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8701 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8761 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8821 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//