LOCUS       RC220617                9219 bp    DNA     circular  SYN 
DEFINITION  PTCH1 (Myc-DDK-tagged)-Human patched 1 (PTCH1), transcript variant 1a'
ACCESSION   NM_001083603
VERSION     NM_001083603.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5376..5467 
                     /label="Myc-DDK"  
     misc_feature    5497..6082 
                     /label="PolyA signal"  
     misc_feature    5554..5573 
                     /label="Restriction Enzyme"  
     misc_feature    6358..7030 
                     /label="ColE1"  
     misc_feature    7460..8254 
                     /label="Kan/Neo"  
     misc_feature    8289..8591 
                     /label="SV40 ori"  
     misc_feature    8677..9154 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5358..5364 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5358 
                     /label="PTCH1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACTGCTT AATAGAAACA GGCTTGTAAT TGTGAGTCCG CGCTGCACTC 
     1081 CGCCGAAAGC CTCCGGCGGC CCAGCGCGCC GGGGTTTTTA CACTTTCCGT TCCTTTTGTA 
     1141 AAGACGGAGG AGGAGGAGAA GAAGAAGAAG AAAACGGAGG AGAAGAAAAA GACGACAGGG 
     1201 GAGACAAAGA GACCCGCAGC GACAAGGGGA AGGCTACTGG CCGGAAAGCG CCGCTGTGGC 
     1261 TGAGAGCGAA GTTTCAGAGA CTCTTATTTA AACTGGGTTG TTACATTCAA AAAAACTGCG 
     1321 GCAAGTTCTT GGTTGTGGGC CTCCTCATAT TTGGGGCCTT CGCGGTGGGA TTAAAAGCAG 
     1381 CGAACCTCGA GACCAACGTG GAGGAGCTGT GGGTGGAAGT TGGAGGACGA GTAAGTCGTG 
     1441 AATTAAATTA TACTCGCCAG AAGATTGGAG AAGAGGCTAT GTTTAATCCT CAACTCATGA 
     1501 TACAGACCCC TAAAGAAGAA GGTGCTAATG TCCTGACCAC AGAAGCGCTC CTACAACACC 
     1561 TGGACTCGGC ACTCCAGGCC AGCCGTGTCC ATGTATACAT GTACAACAGG CAGTGGAAAT 
     1621 TGGAACATTT GTGTTACAAA TCAGGAGAGC TTATCACAGA AACAGGTTAC ATGGATCAGA 
     1681 TAATAGAATA TCTTTACCCT TGTTTGATTA TTACACCTTT GGACTGCTTC TGGGAAGGGG 
     1741 CGAAATTACA GTCTGGGACA GCATACCTCC TAGGTAAACC TCCTTTGCGG TGGACAAACT 
     1801 TCGACCCTTT GGAATTCCTG GAAGAGTTAA AGAAAATAAA CTATCAAGTG GACAGCTGGG 
     1861 AGGAAATGCT GAATAAGGCT GAGGTTGGTC ATGGTTACAT GGACCGCCCC TGCCTCAATC 
     1921 CGGCCGATCC AGACTGCCCC GCCACAGCCC CCAACAAAAA TTCAACCAAA CCTCTTGATA 
     1981 TGGCCCTTGT TTTGAATGGT GGATGTCATG GCTTATCCAG AAAGTATATG CACTGGCAGG 
     2041 AGGAGTTGAT TGTGGGTGGC ACAGTCAAGA ACAGCACTGG AAAACTCGTC AGCGCCCATG 
     2101 CCCTGCAGAC CATGTTCCAG TTAATGACTC CCAAGCAAAT GTACGAGCAC TTCAAGGGGT 
     2161 ACGAGTATGT CTCACACATC AACTGGAACG AGGACAAAGC GGCAGCCATC CTGGAGGCCT 
     2221 GGCAGAGGAC ATATGTGGAG GTGGTTCATC AGAGTGTCGC ACAGAACTCC ACTCAAAAGG 
     2281 TGCTTTCCTT CACCACCACG ACCCTGGACG ACATCCTGAA ATCCTTCTCT GACGTCAGTG 
     2341 TCATCCGCGT GGCCAGCGGC TACTTACTCA TGCTCGCCTA TGCCTGTCTA ACCATGCTGC 
     2401 GCTGGGACTG CTCCAAGTCC CAGGGTGCCG TGGGGCTGGC TGGCGTCCTG CTGGTTGCAC 
     2461 TGTCAGTGGC TGCAGGACTG GGCCTGTGCT CATTGATCGG AATTTCCTTT AACGCTGCAA 
     2521 CAACTCAGGT TTTGCCATTT CTCGCTCTTG GTGTTGGTGT GGATGATGTT TTTCTTCTGG 
     2581 CCCACGCCTT CAGTGAAACA GGACAGAATA AAAGAATCCC TTTTGAGGAC AGGACCGGGG 
     2641 AGTGCCTGAA GCGCACAGGA GCCAGCGTGG CCCTCACGTC CATCAGCAAT GTCACAGCCT 
     2701 TCTTCATGGC CGCGTTAATC CCAATTCCCG CTCTGCGGGC GTTCTCCCTC CAGGCAGCGG 
     2761 TAGTAGTGGT GTTCAATTTT GCCATGGTTC TGCTCATTTT TCCTGCAATT CTCAGCATGG 
     2821 ATTTATATCG ACGCGAGGAC AGGAGACTGG ATATTTTCTG CTGTTTTACA AGCCCCTGCG 
     2881 TCAGCAGAGT GATTCAGGTT GAACCTCAGG CCTACACCGA CACACACGAC AATACCCGCT 
     2941 ACAGCCCCCC ACCTCCCTAC AGCAGCCACA GCTTTGCCCA TGAAACGCAG ATTACCATGC 
     3001 AGTCCACTGT CCAGCTCCGC ACGGAGTACG ACCCCCACAC GCACGTGTAC TACACCACCG 
     3061 CTGAGCCGCG CTCCGAGATC TCTGTGCAGC CCGTCACCGT GACACAGGAC ACCCTCAGCT 
     3121 GCCAGAGCCC AGAGAGCACC AGCTCCACAA GGGACCTGCT CTCCCAGTTC TCCGACTCCA 
     3181 GCCTCCACTG CCTCGAGCCC CCCTGTACGA AGTGGACACT CTCATCTTTT GCTGAGAAGC 
     3241 ACTATGCTCC TTTCCTCTTG AAACCAAAAG CCAAGGTAGT GGTGATCTTC CTTTTTCTGG 
     3301 GCTTGCTGGG GGTCAGCCTT TATGGCACCA CCCGAGTGAG AGACGGGCTG GACCTTACGG 
     3361 ACATTGTACC TCGGGAAACC AGAGAATATG ACTTTATTGC TGCACAATTC AAATACTTTT 
     3421 CTTTCTACAA CATGTATATA GTCACCCAGA AAGCAGACTA CCCGAATATC CAGCACTTAC 
     3481 TTTACGACCT ACACAGGAGT TTCAGTAACG TGAAGTATGT CATGTTGGAA GAAAACAAAC 
     3541 AGCTTCCCAA AATGTGGCTG CACTACTTCA GAGACTGGCT TCAGGGACTT CAGGATGCAT 
     3601 TTGACAGTGA CTGGGAAACC GGGAAAATCA TGCCAAACAA TTACAAGAAT GGATCAGACG 
     3661 ATGGAGTCCT TGCCTACAAA CTCCTGGTGC AAACCGGCAG CCGCGATAAG CCCATCGACA 
     3721 TCAGCCAGTT GACTAAACAG CGTCTGGTGG ATGCAGATGG CATCATTAAT CCCAGCGCTT 
     3781 TCTACATCTA CCTGACGGCT TGGGTCAGCA ACGACCCCGT CGCGTATGCT GCCTCCCAGG 
     3841 CCAACATCCG GCCACACCGA CCAGAATGGG TCCACGACAA AGCCGACTAC ATGCCTGAAA 
     3901 CAAGGCTGAG AATCCCGGCA GCAGAGCCCA TCGAGTATGC CCAGTTCCCT TTCTACCTCA 
     3961 ACGGCTTGCG GGACACCTCA GACTTTGTGG AGGCAATTGA AAAAGTAAGG ACCATCTGCA 
     4021 GCAACTATAC GAGCCTGGGG CTGTCCAGTT ACCCCAACGG CTACCCCTTC CTCTTCTGGG 
     4081 AGCAGTACAT CGGCCTCCGC CACTGGCTGC TGCTGTTCAT CAGCGTGGTG TTGGCCTGCA 
     4141 CATTCCTCGT GTGCGCTGTC TTCCTTCTGA ACCCCTGGAC GGCCGGGATC ATTGTGATGG 
     4201 TCCTGGCGCT GATGACGGTC GAGCTGTTCG GCATGATGGG CCTCATCGGA ATCAAGCTCA 
     4261 GTGCCGTGCC CGTGGTCATC CTGATCGCTT CTGTTGGCAT AGGAGTGGAG TTCACCGTTC 
     4321 ACGTTGCTTT GGCCTTTCTG ACGGCCATCG GCGACAAGAA CCGCAGGGCT GTGCTTGCCC 
     4381 TGGAGCACAT GTTTGCACCC GTCCTGGATG GCGCCGTGTC CACTCTGCTG GGAGTGCTGA 
     4441 TGCTGGCGGG ATCTGAGTTC GACTTCATTG TCAGGTATTT CTTTGCTGTG CTGGCGATCC 
     4501 TCACCATCCT CGGCGTTCTC AATGGGCTGG TTTTGCTTCC CGTGCTTTTG TCTTTCTTTG 
     4561 GACCATATCC TGAGGTGTCT CCAGCCAACG GCTTGAACCG CCTGCCCACA CCCTCCCCTG 
     4621 AGCCACCCCC CAGCGTGGTC CGCTTCGCCA TGCCGCCCGG CCACACGCAC AGCGGGTCTG 
     4681 ATTCCTCCGA CTCGGAGTAT AGTTCCCAGA CGACAGTGTC AGGCCTCAGC GAGGAGCTTC 
     4741 GGCACTACGA GGCCCAGCAG GGCGCGGGAG GCCCTGCCCA CCAAGTGATC GTGGAAGCCA 
     4801 CAGAAAACCC CGTCTTCGCC CACTCCACTG TGGTCCATCC CGAATCCAGG CATCACCCAC 
     4861 CCTCGAACCC GAGACAGCAG CCCCACCTGG ACTCAGGGTC CCTGCCTCCC GGACGGCAAG 
     4921 GCCAGCAGCC CCGCAGGGAC CCCCCCAGAG AAGGCTTGTG GCCACCCCCC TACAGACCGC 
     4981 GCAGAGACGC TTTTGAAATT TCTACTGAAG GGCATTCTGG CCCTAGCAAT AGGGCCCGCT 
     5041 GGGGCCCTCG CGGGGCCCGT TCTCACAACC CTCGGAACCC AGCGTCCACT GCCATGGGCA 
     5101 GCTCCGTGCC CGGCTACTGC CAGCCCATCA CCACTGTGAC GGCTTCTGCC TCCGTGACTG 
     5161 TCGCCGTGCA CCCGCCGCCT GTCCCTGGGC CTGGGCGGAA CCCCCGAGGG GGACTCTGCC 
     5221 CAGGCTACCC TGAGACTGAC CACGGCCTGT TTGAGGACCC CCACGTGCCT TTCCACGTCC 
     5281 GGTGTGAGAG GAGGGATTCG AAGGTGGAAG TCATTGAGCT GCAGGACGTG GAATGCGAGG 
     5341 AGAGGCCCCG GGGAAGCAGC TCCAACACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     5401 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5461 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5521 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5581 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5641 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5701 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5761 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5821 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5881 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5941 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     6001 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     6061 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     6121 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     6181 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     6241 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     6301 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6361 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6421 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6481 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6541 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6601 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6661 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6721 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6781 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6841 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6901 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6961 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     7021 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     7081 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     7141 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     7201 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     7261 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     7321 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     7381 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7441 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7501 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7561 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7621 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7681 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7741 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7801 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7861 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7921 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7981 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     8041 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     8101 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     8161 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     8221 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     8281 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     8341 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     8401 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8461 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8521 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8581 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8641 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8701 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8761 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8821 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8881 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8941 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     9001 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     9061 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     9121 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     9181 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//