LOCUS       RC220614                6975 bp    DNA     circular  SYN 
DEFINITION  ARHGAP22 (Myc-DDK-tagged)-Human Rho GTPase activating protein 22 (ARHGAP22)
ACCESSION   NM_021226
VERSION     NM_021226.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3132..3223 
                     /label="Myc-DDK"  
     misc_feature    3253..3838 
                     /label="PolyA signal"  
     misc_feature    3310..3329 
                     /label="Restriction Enzyme"  
     misc_feature    4114..4786 
                     /label="ColE1"  
     misc_feature    5216..6010 
                     /label="Kan/Neo"  
     misc_feature    6045..6347 
                     /label="SV40 ori"  
     misc_feature    6433..6910 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3114..3120 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3114 
                     /label="ARHGAP22"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGAGCCCA AAGATCAGGC AGGCCAGGAG GGCCCGCTCC AAAAGCCTAG 
     1081 TGATGGGGGA GCAGAGCCGG AGCCCTGGGC GGATGCCGTG CCCTCACAGG CTGGGCCCCG 
     1141 TGCTGAAGGC GGGCTGGCTG AAGAAGCAGA GGAGCATCAT GAAGAACTGG CAGCAGCGCT 
     1201 GGTTTGTGCT GCGTGGGGAT CAGCTTTTCT ACTACAAGGA CAAAGATGAG ATCAAGCCCC 
     1261 AGGGATTTAT TTCTCTACAA GGGACACAGG TGACTGAACT TCCTCCTGGC CCCGAGGACC 
     1321 CAGGGAAGCA CCTCTTTGAG ATCAGCCCAG GTGGTGCCGG GGAGCGGGAG AAGGTGCCGG 
     1381 CCAACCCCGA GGCGCTCCTG CTCATGGCCA GCTCCCAGCG TGACATGGAG GACTGGGTGC 
     1441 AGGCCATCCG CCGAGTCATC TGGGCCCCGC TGGGCGGAGG GATCTTTGGG CAGCGCCTAG 
     1501 AGGAAACAGT CCACCACGAG CGGAAGTATG GCCCCCGCCT GGCGCCCCTG CTGGTGGAGC 
     1561 AGTGTGTGGA CTTCATCCGG GAGCGCGGGC TCACTGAGGA GGGGCTGTTC CGCATGCCAG 
     1621 GCCAGGCCAA CCTGGTGAGG GACCTGCAGG ATTCCTTCGA CTGTGGGGAG AAGCCACTGT 
     1681 TTGACAGCAC AACAGACGTG CACACGGTGG CCTCCCTGCT GAAGCTGTAC CTGCGGGAGC 
     1741 TCCCCGAGCC CGTGGTCCCC TTCGCCAGGT ACGAGGACTT CCTCAGCTGC GCCCAGCTGC 
     1801 TCACCAAGGA CGAGGGGGAG GGCACTCTGG AGTTGGCTAA ACAAGTGAGC AACCTTCCTC 
     1861 AGGCAAATTA CAACCTGCTC AGATACATCT GCAAGTTTCT GGATGAAGTT CAGGCATACT 
     1921 CAAATGTCAA CAAGATGAGT GTCCAGAATC TGGCAACCGT TTTTGGACCT AACATTCTGC 
     1981 GGCCACAGGT AGAGGACCCA GTAACCATCA TGGAAGGCAC TTCCCTCGTC CAGCACCTGA 
     2041 TGACCGTCCT CATCCGCAAA CACAGCCAGC TCTTCACGGC ACCGGTCCCG GAAGGGCCCA 
     2101 CCTCCCCGCG CGGGGGCCTG CAATGCGCAG TGGGGTGGGG CTCCGAGGAG GTCACCAGGG 
     2161 ACAGCCAAGG AGAGCCCGGC GGCCCCGGCC TGCCCGCGCA CAGGACCTCT TCCCTGGACG 
     2221 GGGCGGCCGT GGCGGTGCTC TCCAGAACAG CCCCCACGGG GCCGGGGAGC CGGTGCAGCC 
     2281 CTGGGAAGAA GGTGCAGACC CTGCCCAGTT GGAAGTCCTC CTTCCGGCAG CCGAGGTCCC 
     2341 TATCGGGAAG CCCGAAGGGG GGCGGCTCAT CCCTGGAGGT GCCCATCATC TCCTCCGGCG 
     2401 GGAACTGGCT TATGAACGGG CTGTCCTCCC TGCGCGGACA CCGCCGGGCC TCGTCGGGAG 
     2461 ACCGGCTCAA GGACTCGGGC TCCGTGCAGA GACTCTCCAC CTACGACAAT GTGCCCGCGC 
     2521 CGGGCCTGGT CCCCGGCATA CCCAGCGTGG CCAGTATGGC GTGGTCCGGG GCCTCGTCCA 
     2581 GCGAGTCGTC GGTGGGGGGC TCACTCAGCA GCTGCACGGC CTGCCGCGCC AGCGACTCGT 
     2641 CTGCCCGCAG TTCCCTGCAC ACCGACTGGG CCCTGGAGCC CTCCCCGCTC CCCAGCAGCA 
     2701 GCGAGGACCC CAAGTCCCTG GACCTGGACC ACAGCATGGA CGAGGCGGGC GCGGGTGCCA 
     2761 GCAACAGCGA GCCCAGCGAG CCGGACAGCC CCACCCGGGA ACACGCGCGC CGCTCCGAGG 
     2821 CCTTACAGGG GCTGGTCACT GAGCTCAGGG CCGAGCTGTG CCGCCAGCGG ACTGAGTACG 
     2881 AGAGGAGTGT GAAAAGAATC GAAGAAGGGA GTGCTGACCT GAGAAAACGA ATGTCCCGGT 
     2941 TAGAAGAAGA ACTGGACCAG GAAAAGAAAA AATACATCAT GCTGGAAATA AAGCTGCGGA 
     3001 ACTCTGAACG GGCGCGGGAG GATGCGGAGA GGAGGAACCA GCTGTTGCAG AGGGAAATGG 
     3061 AGGAGTTTTT TTCGACCCTA GGAAGCTTGA CTGTTGGGGC AAAAGGTGCC AGGGCCCCAA 
     3121 AGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3181 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3241 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3301 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3361 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3421 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3481 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     3541 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     3601 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     3661 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     3721 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     3781 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     3841 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     3901 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     3961 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4021 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4081 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4141 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4201 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4261 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4321 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4381 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     4441 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     4501 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     4561 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     4621 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     4681 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     4741 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     4801 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     4861 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     4921 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     4981 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5041 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5101 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5161 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5221 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5281 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5341 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     5401 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     5461 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     5521 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     5581 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     5641 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     5701 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     5761 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     5821 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     5881 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     5941 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6001 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6061 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6121 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6181 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6241 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6301 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6361 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     6421 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     6481 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     6541 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     6601 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     6661 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     6721 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     6781 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     6841 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     6901 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     6961 TTTAACGCGA ATTTT
//