LOCUS       RC220606                8646 bp    DNA     circular  SYN 
DEFINITION  CADPS2 (Myc-DDK-tagged)-Human Ca++-dependent secretion activator 2 (CADPS2), transcript variant 2
ACCESSION   NM_001009571
VERSION     NM_001009571.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4803..4894 
                     /label="Myc-DDK"  
     misc_feature    4924..5509 
                     /label="PolyA signal"  
     misc_feature    4981..5000 
                     /label="Restriction Enzyme"  
     misc_feature    5785..6457 
                     /label="ColE1"  
     misc_feature    6887..7681 
                     /label="Kan/Neo"  
     misc_feature    7716..8018 
                     /label="SV40 ori"  
     misc_feature    8104..8581 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4785..4791 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4785 
                     /label="CADPS2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGGACCCG TCTTCCAGCG AAGAGGAGTC GGACGAGGGG CTGGAAGAGG 
     1081 AAAGCCGCGA TGTGCTGGTG GCAGCCGGCA GCTCGCAGCG AGCTCCTCCA GCCCCGACTC 
     1141 GGGAAGGGCG GCGGGACGCG CCGGGGCGCG CGGGCGGCGG CGGCGCGGCC AGATCTGTGA 
     1201 GCCCGAGCCC CTCTGTGCTC AGCGAGGGGC GAGACGAGCC CCAGCGGCAG CTGGACGATG 
     1261 AGCAGGAGCG GAGGATCCGC CTGCAGCTCT ACGTCTTCGT CGTGAGGTGC ATCGCGTACC 
     1321 CCTTCAACGC CAAGCAGCCC ACCGACATGG CCCGGAGGCA GCAGAAGCTT AACAAACAAC 
     1381 AGTTGCAGTT ACTGAAAGAA CGGTTCCAGG CCTTCCTCAA TGGGGAAACC CAAATTGTAG 
     1441 CTGACGAAGC ATTTTGCAAC GCAGTTCGGA GTTATTATGA GGTTTTTCTA AAGAGTGACC 
     1501 GAGTGGCCAG AATGGTACAG AGTGGAGGGT GTTCTGCTAA TGACTTCAGA GAAGTATTTA 
     1561 AGAAAAACAT AGAAAAACGT GTGCGGAGTT TGCCAGAAAT AGATGGCTTG AGCAAAGAGA 
     1621 CAGTGTTGAG CTCATGGATA GCCAAATATG ATGCCATTTA CAGAGGTGAA GAGGACTTGT 
     1681 GCAAACAGCC AAATAGAATG GCCCTAAGTG CAGTGTCTGA ACTTATTCTG AGCAAGGAAC 
     1741 AACTCTATGA AATGTTTCAG CAGATTCTGG GTATTAAAAA ACTGGAACAC CAGCTCCTTT 
     1801 ATAATGCATG TCAGCTGGAT AACGCAGATG AACAAGCAGC CCAGATCAGA AGGGAACTTG 
     1861 ATGGCCGGCT GCAATTGGCA GATAAAATGG CAAAGGAAAG AAAATTCCCC AAATTTATAG 
     1921 CAAAAGATAT GGAGAATATG TATATAGAAG AGTTGCGGTC TTCAGTGAAT TTGCTAATGG 
     1981 CCAATTTGGA AAGTCTTCCA GTTTCGAAAG GTGGTCCGGA ATTTAAATTA CAAAAATTAA 
     2041 AACGTTCACA GAACTCTGCA TTTTTGGACA TAGGAGATGA GAATGAGATT CAGCTGTCAA 
     2101 AGTCCGACGT GGTACTGTCA TTCACCTTAG AGATTGTCAT AATGGAAGTG CAAGGCCTGA 
     2161 AGTCAGTTGC TCCCAATCGA ATTGTTTACT GTACAATGGA AGTGGAAGGA GAAAAACTTC 
     2221 AGACAGACCA GGCCGAAGCC TCAAGGCCAC AATGGGGGAC TCAAGGAGAT TTCACCACCA 
     2281 CCCATCCTCG GCCTGTGGTC AAAGTGAAAC TCTTCACAGA AAGCACTGGA GTTCTGGCCC 
     2341 TGGAAGATAA AGAACTGGGA AGGGTGATAT TATACCCAAC TTCTAATAGC TCCAAATCAG 
     2401 CTGAATTACA CCGAATGGTA GTTCCAAAAA ATAGCCAGGA TTCTGACTTA AAAATCAAAC 
     2461 TGGCAGTGCG AATGGATAAA CCAGCACATA TGAAGCATAG TGGATATCTG TATGCCCTTG 
     2521 GACAGAAGGT TTGGAAAAGA TGGAAAAAAC GTTACTTTGT TCTAGTTCAG GTTAGCCAAT 
     2581 ATACCTTTGC TATGTGCAGT TATAGAGAAA AGAAGTCTGA ACCACAAGAA TTAATGCAGC 
     2641 TTGAAGGCTA TACTGTGGAT TATACCGATC CCCACCCAGG CCTTCAGGGT GGTTGTATGT 
     2701 TCTTTAATGC TGTTAAAGAA GGAGATACTG TAATCTTTGC CAGTGATGAT GAACAGGACA 
     2761 GAATATTATG GGTTCAAGCC ATGTATAGGG CCACAGGTCA ATCATATAAA CCAGTTCCTG 
     2821 CAATTCAAAC CCAGAAACTG AATCCTAAAG GAGGAACTCT CCATGCAGAT GCTCAGCTTT 
     2881 ATGCAGATCG TTTTCAGAAA CATGGTATGG ATGAGTTTAT TTCTGCAAAC CCCTGCAAGC 
     2941 TTGATCATGC CTTCCTTTTT AGAATACTCC AGAGGCAGAC TTTGGATCAC AGACTGAATG 
     3001 ATTCCTATTC TTGCTTGGGA TGGTTTAGCC CTGGCCAAGT CTTTGTGTTA GATGAGTACT 
     3061 GTGCCCGTTA TGGTGTGAGA GGCTGTCACA GACATCTCTG CTACCTTGCA GAACTGATGG 
     3121 AACATTCAGA AAATGGTGCT GTCATTGACC CTACCCTGCT CCATTACAGC TTTGCATTCT 
     3181 GTGCCTCTCA TGTGCACGGC AACAGGCCTG ATGGAATTGG GACTGTTTCA GTGGAAGAAA 
     3241 AAGAAAGATT TGAGGAGATA AAAGAGAGAC TCTCTTCCCT TTTAGAAAAT CAGATAAGCC 
     3301 ATTTCAGATA CTGTTTTCCC TTTGGACGAC CTGAAGGTGC TCTAAAAGCT ACACTTTCAT 
     3361 TACTTGAAAG GGTTTTAATG AAAGATATTG CCACTCCCAT ACCAGCAGAA GAGGTGAAGA 
     3421 AAGTGGTCAG AAAATGTCTC GAGAAAGCTG CCTTGATCAA TTACACTAGA CTCACAGAAT 
     3481 ATGCCAAAAT AGAAGAGACC ATGAACCAGG CATCTCCTGC TAGAAAGCTG GAAGAGATTC 
     3541 TTCATCTGGC AGAGCTCTGC ATAGAAGTCT TACAGCAGAA TGAAGAGCAT CATGCAGAGG 
     3601 CATTTGCCTG GTGGCCTGAT TTATTGGCTG AACATGCAGA GAAATTTTGG GCTTTATTTA 
     3661 CAGTGGATAT GGACACTGCA CTAGAGGCTC AACCGCAAGA CTCCTGGGAT AGTTTTCCTC 
     3721 TTTTCCAACT GCTTAATAAT TTCCTCCGAA ATGACACACT TTTGTGTAAT GGAAAATTTC 
     3781 ACAAACACTT GCAAGAAATC TTTGTACCCT TGGTTGTCCG CTATGTGGAT CTCATGGAGT 
     3841 CTTCCATCGC CCAGTCAATT CACAGAGGTT TTGAGCAGGA GACATGGCAG CCTGTCAACA 
     3901 ATGGCTCAGC AACATCAGAA GACCTTTTTT GGAAGCTTGA TGCACTGCAA ATGTTTGTCT 
     3961 TTGATCTGCA CTGGCCAGAA CAGGAATTTG CCCACCACTT AGAGCAAAGA CTTAAACTAA 
     4021 TGGCCAGTGA TATGCTAGAG GCCTGTGTCA AAAGAACAAG AACTGCATTT GAACTCAAGC 
     4081 TACAAAAGGC AAGCAAAACA ACTGACTTGC GCATTCCAGC TTCCGTTTGC ACTATGTTTA 
     4141 ATGTATTAGT CGATGCCAAA AAGCAAAGCA CCAAACTCTG TGCCCTGGAT GGAGGACAAG 
     4201 AGTTTGGTAG TCAATGGCAA CAGTACCATT CAAAAATAGA TGATCTGATC GACAACAGTG 
     4261 TAAAAGAAAT CATTTCACTG TTAGTTTCAA AGTTTGTTTC AGTGTTGGAA GGCGTGTTGT 
     4321 CTAAGCTGTC AAGGTATGAT GAAGGCACTT TCTTTTCATC CATTCTGTCA TTCACTGTGA 
     4381 AAGCAGCTGC AAAATATGTT GATGTTCCAA AACCAGGAAT GGATCTGGCA GACACCTATA 
     4441 TTATGTTTGT TCGGCAAAAC CAAGATATTC TTCGAGAAAA GGTCAATGAG GAAATGTATA 
     4501 TAGAAAAGTT ATTTGATCAA TGGTACAGCA GTTCCATGAA AGTCATTTGC GTGTGGTTGA 
     4561 CTGATAGATT AGACCTCCAA CTCCATATTT ACCAGCTGAA GACGCTCATC AAGATTGTGA 
     4621 AGAAAACCTA CAGGGACTTT CGATTGCAGG GTGTGTTGGA AGGAACACTG AACAGTAAGA 
     4681 CTTATGATAC TGTGCACAGA CGTTTAACAG TAGAGGAGGC CACAGCCTCT GTTTCAGAAG 
     4741 GAGGAGGACT TCAGGGCATT ACTATGAAAG ACAGTGACGA AGAAGAAGAA GGCACGCGTA 
     4801 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4861 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4921 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4981 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5041 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5101 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5161 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5221 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5281 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5341 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5401 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5461 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5521 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5581 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5641 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5701 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5761 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5821 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5881 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5941 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6001 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6061 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6121 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6181 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6241 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6301 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6361 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6421 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6481 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6541 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6601 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6661 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6721 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6781 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6841 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6901 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6961 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7021 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7081 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7141 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7201 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7261 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7321 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7381 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7441 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7501 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7561 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7621 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7681 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7741 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7801 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7861 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7921 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7981 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8041 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8101 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8161 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8221 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8281 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8341 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8401 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8461 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8521 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8581 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8641 AATTTT
//